Ašanin, Ružica

Link to this page

Authority KeyName Variants
a26b38d4-fcd4-4999-8d1e-13d11a7703b6
  • Ašanin, Ružica (2)
Projects
No records found.

Author's Bibliography

Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs

Stanojković, Aleksandar; Ašanin, Ružica; Ašanin, Jelena; Palić, Ksenija; Stanojković, Aleksandra; Žutić, Jadranka

(Fakultet veterinarske medicine, Beograd, Srbija, 2011)

TY  - JOUR
AU  - Stanojković, Aleksandar
AU  - Ašanin, Ružica
AU  - Ašanin, Jelena
AU  - Palić, Ksenija
AU  - Stanojković, Aleksandra
AU  - Žutić, Jadranka
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1931
AB  - Cilj ovog rada je bilo ispitivanje prisustva i zastupljenosti streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od zdravih, zaklanih i uginulih svinja različitih kategorija sa nekoliko farmi i klanica u Republici Srbiji. Ukupno je ispitano 226 uzoraka briseva tonzila i nosa od klinički zdrave prasadi u tovu, briseva tonzila prasadi starosti 5-20 dana, delova nazofaringealnih tonzila tovljenika zaklanih na klanici, delova nazofaringealnih tonzila prasadi zaklanih na klanici, briseva sa trupova zaklanih svinja na klanici, briseva sa no`eva za evisceraciju organa na klanici, kao i briseva pluća, trbušne duplje i organa naglo uginule prasadi. Ispitivanje prisustva navedenih vrsta bakterija vršeno je primenom standardnih mikrobioloških metoda. Za identifikaciju izolovanih vrsta bakterija korišćeni su komercijalni biohemijski testovi, a za utvrđivanje serotipske pripadnosti izolovanih sojeva Streptococcus suis specifični serumi za kapsularne antigene. Ustanovljeno je da najveći broj izolovanih sojeva pripada rodu Streptococcus, a u daleko manjem broju izolovane bakterije su pripadale rodovima: Enterococcus (6) (10,4%), Aerococcus (3) (6,2%), Lactococcus (2) (4,2%) i Globicatella (2) (4,2%). Od navede nog broja Streptococcus vrsta najveći broj je pripadao vrsti Streptococcus suis (23) (64%). U brisevima nazofaringealnih tonzila utvrđeno je prisustvo i ostalih á hemolitičkih streptokoka: Streptococcus sanguinis (13,8%), Streptococcus salivarius (5,6%), Streptococcus mitis (5,6%), Streptococcus parasanguinis (2,7%) i Streptococcus oralis (2,7%). Takođe, u manjem procentu izolovana je i vrsta Streptococcus bovis (5,6%). Najveći procenat izolovanih bakterija iz roda Enterococcus pripadao je vrsti Enterococcus faecalis (80%), dok je vrsti Enterococcus faecium pripadao znatno manji procenat izolata (20%). Od ostalih takozvanih streptokokama sličnih vrsta bakterija izolovane su: Aerococcus viridans, Globicatella sanguinis i Lactococcus lactis ssp. cremoris.
AB  - The aim of this investigation was to establish the presence and prevalence of streptococci, enterococci and streptococci-like bacteria in various materials originating from healthy, slaughtered and dead pigs belonging to different categories from several farms and slaughterhouses in the Republic of Serbia. The total number of investigated samples comprised 226 swabs of tonsils and noses from clinically healthy breeders, swabs of tonsils from piglets 5-20 days old, parts of nasopharyngeal tonsils from breeders slaughtered in a slaughterhouse, parts of nasopharyngeal tonsils from piglets slaughtered in a slaughterhouse, swabs of slaughtered pig carcasses from a slaughterhouse, swabs from knives for evisceration in a slaughterhouse, as well as swabs of lungs, abdominal cavity and organs from piglets which died suddenly. The standard microbiological methods were used for investigations of the presence of the listed microorganisms. Commercial biochemical tests were used for the identification of the isolated bacteria and specific sera for capsular antigenes were used for serological determination of the isolated S. suis strains. It was established that the great majority of the isolated strains belonged to the genus Streptococcus (36) (75%), and the minority of the strains belonged to the following genera: Enterococcus (6) (10.4%), Aerococcus (3) (6.2%), Lactococcus (2) (4.2%) and Globicatella (2) (4.2%). The great majority of Streptococcus species belonged to S. suis. The presence of other á haemolytic streptococci was established in the swabs of nasopharyngeal tonsils: Streptococcus sanguinis (13.8%), Streptococcus salivarius (5.6%), Streptococcus mitis (5.6%), Streptococcus parasanguinis (2.7%) and Streptococcus oralis (2.7%). Also, S. bovis was isolated in a smaller percentage (5.6%). The greatest number of isolated bacteria from the genus Enterococcus belonged to Enterococcus faecalis (80%), while the minority of isolated strains belonged to Enterococcus faecium (20%). The following from the streptococci-like bacteria were isolated: Aerococcus viridans, Globicatella sanguinis and Lactococcus lactis ssp.cremoris.
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs
T1  - Ispitivanje prisustva alfa hemolitičkih streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od svinja
VL  - 65
IS  - 3-4
SP  - 203
EP  - 213
DO  - 10.2298/VETGL1104203S
ER  - 
@article{
author = "Stanojković, Aleksandar and Ašanin, Ružica and Ašanin, Jelena and Palić, Ksenija and Stanojković, Aleksandra and Žutić, Jadranka",
year = "2011",
abstract = "Cilj ovog rada je bilo ispitivanje prisustva i zastupljenosti streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od zdravih, zaklanih i uginulih svinja različitih kategorija sa nekoliko farmi i klanica u Republici Srbiji. Ukupno je ispitano 226 uzoraka briseva tonzila i nosa od klinički zdrave prasadi u tovu, briseva tonzila prasadi starosti 5-20 dana, delova nazofaringealnih tonzila tovljenika zaklanih na klanici, delova nazofaringealnih tonzila prasadi zaklanih na klanici, briseva sa trupova zaklanih svinja na klanici, briseva sa no`eva za evisceraciju organa na klanici, kao i briseva pluća, trbušne duplje i organa naglo uginule prasadi. Ispitivanje prisustva navedenih vrsta bakterija vršeno je primenom standardnih mikrobioloških metoda. Za identifikaciju izolovanih vrsta bakterija korišćeni su komercijalni biohemijski testovi, a za utvrđivanje serotipske pripadnosti izolovanih sojeva Streptococcus suis specifični serumi za kapsularne antigene. Ustanovljeno je da najveći broj izolovanih sojeva pripada rodu Streptococcus, a u daleko manjem broju izolovane bakterije su pripadale rodovima: Enterococcus (6) (10,4%), Aerococcus (3) (6,2%), Lactococcus (2) (4,2%) i Globicatella (2) (4,2%). Od navede nog broja Streptococcus vrsta najveći broj je pripadao vrsti Streptococcus suis (23) (64%). U brisevima nazofaringealnih tonzila utvrđeno je prisustvo i ostalih á hemolitičkih streptokoka: Streptococcus sanguinis (13,8%), Streptococcus salivarius (5,6%), Streptococcus mitis (5,6%), Streptococcus parasanguinis (2,7%) i Streptococcus oralis (2,7%). Takođe, u manjem procentu izolovana je i vrsta Streptococcus bovis (5,6%). Najveći procenat izolovanih bakterija iz roda Enterococcus pripadao je vrsti Enterococcus faecalis (80%), dok je vrsti Enterococcus faecium pripadao znatno manji procenat izolata (20%). Od ostalih takozvanih streptokokama sličnih vrsta bakterija izolovane su: Aerococcus viridans, Globicatella sanguinis i Lactococcus lactis ssp. cremoris., The aim of this investigation was to establish the presence and prevalence of streptococci, enterococci and streptococci-like bacteria in various materials originating from healthy, slaughtered and dead pigs belonging to different categories from several farms and slaughterhouses in the Republic of Serbia. The total number of investigated samples comprised 226 swabs of tonsils and noses from clinically healthy breeders, swabs of tonsils from piglets 5-20 days old, parts of nasopharyngeal tonsils from breeders slaughtered in a slaughterhouse, parts of nasopharyngeal tonsils from piglets slaughtered in a slaughterhouse, swabs of slaughtered pig carcasses from a slaughterhouse, swabs from knives for evisceration in a slaughterhouse, as well as swabs of lungs, abdominal cavity and organs from piglets which died suddenly. The standard microbiological methods were used for investigations of the presence of the listed microorganisms. Commercial biochemical tests were used for the identification of the isolated bacteria and specific sera for capsular antigenes were used for serological determination of the isolated S. suis strains. It was established that the great majority of the isolated strains belonged to the genus Streptococcus (36) (75%), and the minority of the strains belonged to the following genera: Enterococcus (6) (10.4%), Aerococcus (3) (6.2%), Lactococcus (2) (4.2%) and Globicatella (2) (4.2%). The great majority of Streptococcus species belonged to S. suis. The presence of other á haemolytic streptococci was established in the swabs of nasopharyngeal tonsils: Streptococcus sanguinis (13.8%), Streptococcus salivarius (5.6%), Streptococcus mitis (5.6%), Streptococcus parasanguinis (2.7%) and Streptococcus oralis (2.7%). Also, S. bovis was isolated in a smaller percentage (5.6%). The greatest number of isolated bacteria from the genus Enterococcus belonged to Enterococcus faecalis (80%), while the minority of isolated strains belonged to Enterococcus faecium (20%). The following from the streptococci-like bacteria were isolated: Aerococcus viridans, Globicatella sanguinis and Lactococcus lactis ssp.cremoris.",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs, Ispitivanje prisustva alfa hemolitičkih streptokoka, enterokoka i streptokokama sličnih bakterija u različitim materijalima poreklom od svinja",
volume = "65",
number = "3-4",
pages = "203-213",
doi = "10.2298/VETGL1104203S"
}
Stanojković, A., Ašanin, R., Ašanin, J., Palić, K., Stanojković, A.,& Žutić, J.. (2011). Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 65(3-4), 203-213.
https://doi.org/10.2298/VETGL1104203S
Stanojković A, Ašanin R, Ašanin J, Palić K, Stanojković A, Žutić J. Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs. in Veterinarski Glasnik. 2011;65(3-4):203-213.
doi:10.2298/VETGL1104203S .
Stanojković, Aleksandar, Ašanin, Ružica, Ašanin, Jelena, Palić, Ksenija, Stanojković, Aleksandra, Žutić, Jadranka, "Investigation of presence of α haemolytic streptococci, enterococci and streptococci-like bacteria in different materials originating from pigs" in Veterinarski Glasnik, 65, no. 3-4 (2011):203-213,
https://doi.org/10.2298/VETGL1104203S . .

Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia

Tambur, Zoran; Ašanin, Ružica; Stojanov, Igor; Medenica, Ivica

(Fakultet veterinarske medicine, Beograd, Srbija, 2008)

TY  - JOUR
AU  - Tambur, Zoran
AU  - Ašanin, Ružica
AU  - Stojanov, Igor
AU  - Medenica, Ivica
PY  - 2008
UR  - https://doaj.org/article/c767a470c6c640268bd7308a7c979954
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1953
AB  - Examinations were carried out during the period from January 2006 until March 2007 on a total of 449 samples of the cecum of broilers and the cecum and the colon of pigs. These samples included 251 samples originating from broilers and 198 samples of pig cecums and colons. All the listed samples were obtained by scraping the surface of these parts of the digestive system of broilers and pigs. At the site of sampling, the diluted material was sown on a medium (Karmali agar), in order to get individual colonies. After sowing, the bases were placed in anaerobic jars in which microaerophilic conditions were achieved using Campy Pak, BBL bags. On arrival at the laboratory, the jars containing the sown bases were placed in a thermostat, at a temperature of 42oC for 48 hours for the purpose of incubation. Following incubation, the grown colonies were examined macroscopically, and then microscopic preparations were made from them, which were stained with 2% carbol fuchsin after drying and fixation. Those isolates which were in the form of a comma, the letter S, or gull's wings in the microscopic preparations were considered Campylobacter species (Figures 1 and 2). The isolated thermophilic campylobacteria were identified using conventional and commercial biochemical tests API Campy, manufactured by Bio Mérieux, France. With the application of these microbiological methods, thermophilic Campylobacter species were isolated from 203 (80.88%) of the 251 samples of broiler cecums. Furthermore, thermophilic campylobacteria were isolated from 153 (77.27%) of the 198 samples from the cecum and colon of pigs taken within these investigations. The obtained results indicate that there is a somewhat greater prevalence of these bacteria among the broilers. However, such a high percentage of both broilers and pigs colonized by thermophilic Campylobacter species could pose a serious problem, in particular when it is known that infections of humans caused by the consumption of insufficiently thermally processed meat of broilers or pigs are not infrequent.
AB  - Ispitivanjima obavljenim u periodu od januara 2006. godine do marta 2007. godine ukupno je pregledano 449 uzoraka cekuma brojlera i cekuma i kolona svinja. Od navedenog broja 251 uzorak je poticao od brojlera, a 198 uzoraka iz cekuma i kolona svinja. Svi navedeni uzorci dobijeni su struganjem površine navedenih delova digestivnog sistema brojlera i svinja. Materijal je na mestu uzorkovanja zasejavan sa razređenjem na podlogu (Karmali agar), radi dobijanja pojedinačnih kolonija. Nakon zasejavanja podloge su odlagane u lonce za anaerobe, u kojima su mikroaerofilni uslovi postignuti primenom Campy Pak, BBL kesica. Lonci sa zasejanim podlogama po dopremanju u laboratoriju stavljani su u termostat na temperaturu od 42oC tokom 48 časova radi inkubiranja. Nakon inkubacije izrasle kolonije su pregledane makroskopski, a zatim su od njih pripremani mikroskopski preparati koji su nakon sušenja i fiksiranja bojeni 2% karbol fuksinom. Oni izolati koji su na mikroskopskim preparatima imali oblik zareza, latinskog slova S ili galebovih krila smatrani su Campylobacter vrstama (slika 1 i 2). Identifikacija izolovanih termofilnih kampilobakterija vršena je primenom klasičnih i komercijalnih biohemijskih testova API Campy, proizvođača Bio Mérieux, Francuska. Primenom navedenih mikrobioloških metoda od 251. uzorka iz cekuma brojlera iz 203 (80,88%) izolovane su termofilne Campylobacter vrste. Takođe, od 198 uzoraka iz cekuma i kolona svinja koji su bili obuhvaćeni ispitivanjem termofilne kampilobakterije su izolovane iz 153 (77,27%). Dobijeni rezultati ukazuju na nešto veću prevalenciju ovih bakterija kod brojlera. Međutim, ovako visok procenat kolonizovanih, kako brojlera, tako i svinja termofilnim Campylobacter vrstama može predstavljati ozbiljan problem, posebno ako se zna da infekcije ljudi izazvane konzumiranjem nedovoljno termički obrađenog mesa brojlera i svinja nisu tako retke.
PB  - Fakultet veterinarske medicine, Beograd, Srbija
T2  - Veterinarski Glasnik
T1  - Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia
T1  - Prisustvo termofilnih Campylobacter vrsta kod brojlera i svinja na nekim klanicama u republici Srbiji
VL  - 62
IS  - 1-2
SP  - 77
EP  - 83
DO  - 10.2298/VETGL0802077T
ER  - 
@article{
author = "Tambur, Zoran and Ašanin, Ružica and Stojanov, Igor and Medenica, Ivica",
year = "2008",
abstract = "Examinations were carried out during the period from January 2006 until March 2007 on a total of 449 samples of the cecum of broilers and the cecum and the colon of pigs. These samples included 251 samples originating from broilers and 198 samples of pig cecums and colons. All the listed samples were obtained by scraping the surface of these parts of the digestive system of broilers and pigs. At the site of sampling, the diluted material was sown on a medium (Karmali agar), in order to get individual colonies. After sowing, the bases were placed in anaerobic jars in which microaerophilic conditions were achieved using Campy Pak, BBL bags. On arrival at the laboratory, the jars containing the sown bases were placed in a thermostat, at a temperature of 42oC for 48 hours for the purpose of incubation. Following incubation, the grown colonies were examined macroscopically, and then microscopic preparations were made from them, which were stained with 2% carbol fuchsin after drying and fixation. Those isolates which were in the form of a comma, the letter S, or gull's wings in the microscopic preparations were considered Campylobacter species (Figures 1 and 2). The isolated thermophilic campylobacteria were identified using conventional and commercial biochemical tests API Campy, manufactured by Bio Mérieux, France. With the application of these microbiological methods, thermophilic Campylobacter species were isolated from 203 (80.88%) of the 251 samples of broiler cecums. Furthermore, thermophilic campylobacteria were isolated from 153 (77.27%) of the 198 samples from the cecum and colon of pigs taken within these investigations. The obtained results indicate that there is a somewhat greater prevalence of these bacteria among the broilers. However, such a high percentage of both broilers and pigs colonized by thermophilic Campylobacter species could pose a serious problem, in particular when it is known that infections of humans caused by the consumption of insufficiently thermally processed meat of broilers or pigs are not infrequent., Ispitivanjima obavljenim u periodu od januara 2006. godine do marta 2007. godine ukupno je pregledano 449 uzoraka cekuma brojlera i cekuma i kolona svinja. Od navedenog broja 251 uzorak je poticao od brojlera, a 198 uzoraka iz cekuma i kolona svinja. Svi navedeni uzorci dobijeni su struganjem površine navedenih delova digestivnog sistema brojlera i svinja. Materijal je na mestu uzorkovanja zasejavan sa razređenjem na podlogu (Karmali agar), radi dobijanja pojedinačnih kolonija. Nakon zasejavanja podloge su odlagane u lonce za anaerobe, u kojima su mikroaerofilni uslovi postignuti primenom Campy Pak, BBL kesica. Lonci sa zasejanim podlogama po dopremanju u laboratoriju stavljani su u termostat na temperaturu od 42oC tokom 48 časova radi inkubiranja. Nakon inkubacije izrasle kolonije su pregledane makroskopski, a zatim su od njih pripremani mikroskopski preparati koji su nakon sušenja i fiksiranja bojeni 2% karbol fuksinom. Oni izolati koji su na mikroskopskim preparatima imali oblik zareza, latinskog slova S ili galebovih krila smatrani su Campylobacter vrstama (slika 1 i 2). Identifikacija izolovanih termofilnih kampilobakterija vršena je primenom klasičnih i komercijalnih biohemijskih testova API Campy, proizvođača Bio Mérieux, Francuska. Primenom navedenih mikrobioloških metoda od 251. uzorka iz cekuma brojlera iz 203 (80,88%) izolovane su termofilne Campylobacter vrste. Takođe, od 198 uzoraka iz cekuma i kolona svinja koji su bili obuhvaćeni ispitivanjem termofilne kampilobakterije su izolovane iz 153 (77,27%). Dobijeni rezultati ukazuju na nešto veću prevalenciju ovih bakterija kod brojlera. Međutim, ovako visok procenat kolonizovanih, kako brojlera, tako i svinja termofilnim Campylobacter vrstama može predstavljati ozbiljan problem, posebno ako se zna da infekcije ljudi izazvane konzumiranjem nedovoljno termički obrađenog mesa brojlera i svinja nisu tako retke.",
publisher = "Fakultet veterinarske medicine, Beograd, Srbija",
journal = "Veterinarski Glasnik",
title = "Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia, Prisustvo termofilnih Campylobacter vrsta kod brojlera i svinja na nekim klanicama u republici Srbiji",
volume = "62",
number = "1-2",
pages = "77-83",
doi = "10.2298/VETGL0802077T"
}
Tambur, Z., Ašanin, R., Stojanov, I.,& Medenica, I.. (2008). Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia. in Veterinarski Glasnik
Fakultet veterinarske medicine, Beograd, Srbija., 62(1-2), 77-83.
https://doi.org/10.2298/VETGL0802077T
Tambur Z, Ašanin R, Stojanov I, Medenica I. Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia. in Veterinarski Glasnik. 2008;62(1-2):77-83.
doi:10.2298/VETGL0802077T .
Tambur, Zoran, Ašanin, Ružica, Stojanov, Igor, Medenica, Ivica, "Presence of thermophilic Campylobacter species in Broilers and pigs at certain abattoirs in Republic of Serbia" in Veterinarski Glasnik, 62, no. 1-2 (2008):77-83,
https://doi.org/10.2298/VETGL0802077T . .