TY  - CONF
AU  - Polović, N.
AU  - Bergman, T.
AU  - Milčić-Matić, Natalija
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/737
AB  - Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - a source of dog allergens
VL  - 65
IS  - 92
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1464
ER  - 

@conference{
author = "Polović, N. and Bergman, T. and Milčić-Matić, Natalija and Gronlund, H. and van Hage, M.",
year = "2010",
abstract = "Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - a source of dog allergens",
volume = "65",
number = "92",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1464"
}

Polović, N., Bergman, T., Milčić-Matić, N., Gronlund, H.,& van Hage, M.. (2010). Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 65(92), 105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464

Polović N, Bergman T, Milčić-Matić N, Gronlund H, van Hage M. Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2010;65(92):105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464 .

Polović, N., Bergman, T., Milčić-Matić, Natalija, Gronlund, H., van Hage, M., "Dog saliva - a source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 65, no. 92 (2010):105-105,
https://hdl.handle.net/21.15107/rcub_cherry_1464 .