TY  - JOUR
AU  - Pérez-Ramírez, Elisa
AU  - Cano-Gómez, Cristina
AU  - Llorente, Francisco
AU  - Adžić, Bojan
AU  - Al Ameer, Maisa
AU  - Djadjovski, Igor
AU  - Hage, Jeanne El
AU  - Mellouli, Fatiha El
AU  - Goletic, Teufik
AU  - Hovsepyan, Hermine
AU  - Karayel-Hacioglu, Ilke
AU  - Maksimović-Zorić, Jelena
AU  - Mejri, Selma
AU  - Sadaoui, Hassiba
AU  - Salem, Sayed Hassan
AU  - Sherifi, Kurtesh
AU  - Toklikishvili, Natela
AU  - Vodica, Ani
AU  - Monaco, Federica
AU  - Brun, Alejandro
AU  - Jiménez-Clavero, Miguel Ángel
AU  - Fernández-Pinero, Jovita
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1986
AB  - Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.
PB  - Public Library of Science
T2  - PLoS ONE
T1  - External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions
VL  - 15
DO  - 10.1371/journal.pone.0239478
ER  - 

@article{
author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Adžić, Bojan and Al Ameer, Maisa and Djadjovski, Igor and Hage, Jeanne El and Mellouli, Fatiha El and Goletic, Teufik and Hovsepyan, Hermine and Karayel-Hacioglu, Ilke and Maksimović-Zorić, Jelena and Mejri, Selma and Sadaoui, Hassiba and Salem, Sayed Hassan and Sherifi, Kurtesh and Toklikishvili, Natela and Vodica, Ani and Monaco, Federica and Brun, Alejandro and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita",
year = "2020",
abstract = "Rift Valley fever (RVF) is an arboviral zoonosis that primarily affects ruminants but can also cause illness in humans. The increasing impact of RVF in Africa and Middle East and the risk of expansion to other areas such as Europe, where competent mosquitos are already established, require the implementation of efficient surveillance programs in animal populations. For that, it is pivotal to regularly assess the performance of existing diagnostic tests and to evaluate the capacity of veterinary labs of endemic and non-endemic countries to detect the infection in an accurate and timely manner. In this context, the animal virology network of the MediLabSecure project organized between October 2016 and March 2017 an external quality assessment (EQA) to evaluate the RVF diagnostic capacities of beneficiary veterinary labs. This EQA was conceived as the last step of a training curriculum that included 2 diagnostic workshops that were organized by INIA-CISA (Spain) in 2015 and 2016. Seventeen veterinary diagnostic labs from 17 countries in the Mediterranean and Black Sea regions participated in this EQA. The exercise consisted of two panels of samples for molecular and serological detection of the virus. The laboratories were also provided with positive controls and all the kits and reagents necessary to perform the recommended diagnostic techniques. All the labs were able to apply the different protocols and to provide the results on time. The performance was good in the molecular panel with 70.6% of participants reporting 100% correct results, and excellent in the serological panel with 100% correct results reported by 94.1% of the labs. This EQA provided a good overview of the RVFV diagnostic capacities of the involved labs and demonstrated that most of them were able to correctly identify the virus genome and antibodies in different animal samples.",
publisher = "Public Library of Science",
journal = "PLoS ONE",
title = "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions",
volume = "15",
doi = "10.1371/journal.pone.0239478"
}

Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Adžić, B., Al Ameer, M., Djadjovski, I., Hage, J. E., Mellouli, F. E., Goletic, T., Hovsepyan, H., Karayel-Hacioglu, I., Maksimović-Zorić, J., Mejri, S., Sadaoui, H., Salem, S. H., Sherifi, K., Toklikishvili, N., Vodica, A., Monaco, F., Brun, A., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE
Public Library of Science., 15.
https://doi.org/10.1371/journal.pone.0239478

Pérez-Ramírez E, Cano-Gómez C, Llorente F, Adžić B, Al Ameer M, Djadjovski I, Hage JE, Mellouli FE, Goletic T, Hovsepyan H, Karayel-Hacioglu I, Maksimović-Zorić J, Mejri S, Sadaoui H, Salem SH, Sherifi K, Toklikishvili N, Vodica A, Monaco F, Brun A, Jiménez-Clavero MÁ, Fernández-Pinero J. External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions. in PLoS ONE. 2020;15.
doi:10.1371/journal.pone.0239478 .

Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Adžić, Bojan, Al Ameer, Maisa, Djadjovski, Igor, Hage, Jeanne El, Mellouli, Fatiha El, Goletic, Teufik, Hovsepyan, Hermine, Karayel-Hacioglu, Ilke, Maksimović-Zorić, Jelena, Mejri, Selma, Sadaoui, Hassiba, Salem, Sayed Hassan, Sherifi, Kurtesh, Toklikishvili, Natela, Vodica, Ani, Monaco, Federica, Brun, Alejandro, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "External quality assessment of Rift Valley fever diagnosis in 17 veterinary laboratories of the Mediterranean and Black Sea regions" in PLoS ONE, 15 (2020),
https://doi.org/10.1371/journal.pone.0239478 . .