TY  - JOUR
AU  - Savić, Dejana
AU  - Ristanović, Elizabeta
AU  - Miljković Selimović, Biljana
AU  - Radaković, Sonja
AU  - Jošić, Dragana
AU  - Lepšanović, Zorica
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2403
AB  - Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain.
AB  - Bacillus cereus se najčešće unosi hranom i usled prisustva toksina može izazvati povraćanje i dijareu. Sistemska infekcija je moguća. Cilj je bio da se utvrdi prisustvo gena za enterotoksine (hblA, entFM) i emetični toksin (cer) i istražiti genetsku heterogenost izolata B. cereus iz uzoraka hrane, životne sredine i stolice pacijenata. Identifi kacija B. cereus je urađena upotrebom selektivne hranljive podloge, klasičnih biohemijskih testova i lančane reakcije polimeraze (PCR). Detekcija gena je rađena PCR-om. Tipizacija je urađena pomoću nasumičnog umnožavanja polimorfne DNA (RAPD). EntFM gen je bio prisutan u svakom uzorku stolice i hrane, a u 28/30 izolata iz okoline. HblA gen je bio prisutan u 29/30 izolata stolice, 23/30 izolata hrane i u 24/30 izolata iz okoline. Cer gen je bio prisutan u svim izolatima stolice, 28/30 izolata hrane i 25/30 izolata iz okoline. RAPD rezultati pokazuju visoku heterogenost među izolatima iz svake grupe. Zbirni dendrogram dobijen od odabranih izolata iz sve tri ispitane grupe, pokazuje formiranje dva klastera sa međusobnom razlikom od 53%. Detekcija gena koji kodiraju prisustvo toksina u svim izolatima B. cereus, ukazuje da su ove bakterije potencijalno patogene i predstavljaju ozbiljnu pretnju po ljudsko zdravlje. Prisustvo izolata iz sve tri grupe u istom klasteru ukazuje na postojanje sličnih sojeva iz različitih izvora, što se dovodi u direktnu vezu sa kretanjem sojeva u prirodi kroz lanac ishrane.
T2  - Acta Veterinaria-Beograd
T1  - Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia
T1  - Prisustvo gena za enerotoksine i emetični toksin i genetička raznolikost bakterije bacillus cereus izolovane iz hrane, okoline i kliničkih uzoraka u Srbiji
VL  - 70
IS  - 2
SP  - 182
EP  - 193
DO  - 10.2478/acve-2020-0013
ER  - 

@article{
author = "Savić, Dejana and Ristanović, Elizabeta and Miljković Selimović, Biljana and Radaković, Sonja and Jošić, Dragana and Lepšanović, Zorica",
year = "2020",
abstract = "Bacillus cereus, usually ingested by food, can cause two types of disease due to the presence of toxins: vomiting and diarrhea syndrome. Systemic infections can also occur. The aim was to detect genes for enterotoxins (hblA, entFM) and emetic toxin (cer) and to investigate the genetic heterogeneity of B. cereus isolates from food, environment and human stool. Identification of B. cereus was performed by means of selective medium, classical biochemical test and polymerase chain reaction (PCR). Toxin genes were detected by PCR. Typing was performed by random amplified polymorphic DNA (RAPD). EntFM gene was present in all stool and food samples and in 28/30 environmental isolates. HblA gene was present in 29/30 stool, 23/30 food and 24/30 environmental isolates. Cer gene was present in 30/30 stool, 28/30 food and 25/30 environmental isolates. The RAPD results show high heterogeneity among the isolates from each group. In the cumulative dendrogram, representative isolates from all three groups formed two clusters with a difference of 53%. The detection of toxin genes in all B. cereus isolates indicated these bacteria as potentially pathogenic and a serious threat for human health. The presence of isolates from all three groups in the same cluster suggests the existence of similar strains in the environment, food and patients, which is in line with the circulation of strains in nature through the food chain., Bacillus cereus se najčešće unosi hranom i usled prisustva toksina može izazvati povraćanje i dijareu. Sistemska infekcija je moguća. Cilj je bio da se utvrdi prisustvo gena za enterotoksine (hblA, entFM) i emetični toksin (cer) i istražiti genetsku heterogenost izolata B. cereus iz uzoraka hrane, životne sredine i stolice pacijenata. Identifi kacija B. cereus je urađena upotrebom selektivne hranljive podloge, klasičnih biohemijskih testova i lančane reakcije polimeraze (PCR). Detekcija gena je rađena PCR-om. Tipizacija je urađena pomoću nasumičnog umnožavanja polimorfne DNA (RAPD). EntFM gen je bio prisutan u svakom uzorku stolice i hrane, a u 28/30 izolata iz okoline. HblA gen je bio prisutan u 29/30 izolata stolice, 23/30 izolata hrane i u 24/30 izolata iz okoline. Cer gen je bio prisutan u svim izolatima stolice, 28/30 izolata hrane i 25/30 izolata iz okoline. RAPD rezultati pokazuju visoku heterogenost među izolatima iz svake grupe. Zbirni dendrogram dobijen od odabranih izolata iz sve tri ispitane grupe, pokazuje formiranje dva klastera sa međusobnom razlikom od 53%. Detekcija gena koji kodiraju prisustvo toksina u svim izolatima B. cereus, ukazuje da su ove bakterije potencijalno patogene i predstavljaju ozbiljnu pretnju po ljudsko zdravlje. Prisustvo izolata iz sve tri grupe u istom klasteru ukazuje na postojanje sličnih sojeva iz različitih izvora, što se dovodi u direktnu vezu sa kretanjem sojeva u prirodi kroz lanac ishrane.",
journal = "Acta Veterinaria-Beograd",
title = "Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia, Prisustvo gena za enerotoksine i emetični toksin i genetička raznolikost bakterije bacillus cereus izolovane iz hrane, okoline i kliničkih uzoraka u Srbiji",
volume = "70",
number = "2",
pages = "182-193",
doi = "10.2478/acve-2020-0013"
}

Savić, D., Ristanović, E., Miljković Selimović, B., Radaković, S., Jošić, D.,& Lepšanović, Z.. (2020). Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia. in Acta Veterinaria-Beograd, 70(2), 182-193.
https://doi.org/10.2478/acve-2020-0013

Savić D, Ristanović E, Miljković Selimović B, Radaković S, Jošić D, Lepšanović Z. Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia. in Acta Veterinaria-Beograd. 2020;70(2):182-193.
doi:10.2478/acve-2020-0013 .

Savić, Dejana, Ristanović, Elizabeta, Miljković Selimović, Biljana, Radaković, Sonja, Jošić, Dragana, Lepšanović, Zorica, "Enterotoxin and emetic toxin genes profiles and genetic diversity of bacillus cereus isolated from food, environmental and clinical samples in Serbia" in Acta Veterinaria-Beograd, 70, no. 2 (2020):182-193,
https://doi.org/10.2478/acve-2020-0013 . .

TY  - JOUR
AU  - Lepsanović, Zorica
AU  - Đorđević, Vesna
AU  - Lakićević, Brankica
AU  - Savić, Dejana
AU  - Velebit, Branko
AU  - Josić, Dragana
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1659
AB  - Bacillus cereus, a ubiquitous human foodborne pathogen, can persist in different environment, including dry conditions. In this study, we explored the genetic diversity of B. cereus isolates from infant milk formulas. Out of 83 samples, B. cereus was confirmed in 26 (31.3%). Isolates were resistant to penicillin G and ampicillin (100%), trimethoprim-sulfamethoxazole (84.6%), and erythromycin (3.8%). Ent, hbl, and cer genes were detected in 21 (80.8%), 2 (7.7%), and 11 (42.3%) isolates, respectively. Randomly amplified polymorphic DNA (RAPD) analysis, using set of three primers, provided an overall genetic distance of 95% between B. cereus isolates. A group of seven emetic strains and another two enterotoxic strains were indistinguishable by RAPD, revealing their high clonality. In conclusion, we detected a high proportion of pathogenic B. cereus isolates in infant milk formulas. The majority of isolates harboring both cer and ent genes showed high clonality, pointing food processing environment as probable contamination source. Practical applicationsDetection of Bacillus cereus in powdered infant milk formulas, the prevalence of emetic and diarrhegenic genes, and especially the simultaneous presence of both types of genes in the same isolate, confirm the importance of B. cereus as foodborne pathogen. The consumption of contaminated formula may cause serious health problems in babies, which emphasizes the need to improve B. cereus monitoring and characterization. The clonal relationship between strains, revealed by RAPD analysis, may point to the source of infection and the corresponding prevention measures to improve food safety. Moreover, the identical RAPD profile of cer positive strains obtained with AG15 primer enables the application of this analysis in screening for emetic B. cereus isolates.
PB  - Wiley, Hoboken
T2  - Journal of Food Safety
T1  - Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas
VL  - 38
IS  - 4
SP  - e12474
DO  - 10.1111/jfs.12474
ER  - 

@article{
author = "Lepsanović, Zorica and Đorđević, Vesna and Lakićević, Brankica and Savić, Dejana and Velebit, Branko and Josić, Dragana and Karabasil, Nedjeljko",
year = "2018",
abstract = "Bacillus cereus, a ubiquitous human foodborne pathogen, can persist in different environment, including dry conditions. In this study, we explored the genetic diversity of B. cereus isolates from infant milk formulas. Out of 83 samples, B. cereus was confirmed in 26 (31.3%). Isolates were resistant to penicillin G and ampicillin (100%), trimethoprim-sulfamethoxazole (84.6%), and erythromycin (3.8%). Ent, hbl, and cer genes were detected in 21 (80.8%), 2 (7.7%), and 11 (42.3%) isolates, respectively. Randomly amplified polymorphic DNA (RAPD) analysis, using set of three primers, provided an overall genetic distance of 95% between B. cereus isolates. A group of seven emetic strains and another two enterotoxic strains were indistinguishable by RAPD, revealing their high clonality. In conclusion, we detected a high proportion of pathogenic B. cereus isolates in infant milk formulas. The majority of isolates harboring both cer and ent genes showed high clonality, pointing food processing environment as probable contamination source. Practical applicationsDetection of Bacillus cereus in powdered infant milk formulas, the prevalence of emetic and diarrhegenic genes, and especially the simultaneous presence of both types of genes in the same isolate, confirm the importance of B. cereus as foodborne pathogen. The consumption of contaminated formula may cause serious health problems in babies, which emphasizes the need to improve B. cereus monitoring and characterization. The clonal relationship between strains, revealed by RAPD analysis, may point to the source of infection and the corresponding prevention measures to improve food safety. Moreover, the identical RAPD profile of cer positive strains obtained with AG15 primer enables the application of this analysis in screening for emetic B. cereus isolates.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Safety",
title = "Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas",
volume = "38",
number = "4",
pages = "e12474",
doi = "10.1111/jfs.12474"
}

Lepsanović, Z., Đorđević, V., Lakićević, B., Savić, D., Velebit, B., Josić, D.,& Karabasil, N.. (2018). Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas. in Journal of Food Safety
Wiley, Hoboken., 38(4), e12474.
https://doi.org/10.1111/jfs.12474

Lepsanović Z, Đorđević V, Lakićević B, Savić D, Velebit B, Josić D, Karabasil N. Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas. in Journal of Food Safety. 2018;38(4):e12474.
doi:10.1111/jfs.12474 .

Lepsanović, Zorica, Đorđević, Vesna, Lakićević, Brankica, Savić, Dejana, Velebit, Branko, Josić, Dragana, Karabasil, Nedjeljko, "Detection of toxin genes and randomly amplified polymorphic DNA typing of Bacillus cereus isolates from infant milk formulas" in Journal of Food Safety, 38, no. 4 (2018):e12474,
https://doi.org/10.1111/jfs.12474 . .