TY  - JOUR
AU  - Aleksić-Kovačević, Sanja
AU  - Kukolj, Vladimir
AU  - Kureljušić, Branislav
AU  - Marinković, Darko
AU  - Knežević, Đ.
AU  - Ignjatović, I.
AU  - Jovanović, M.
AU  - Knežević, Milijana
AU  - Gledić, Dušan
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/686
AB  - Hepatic stellate cells (HSCs) play a significant role in hepatic fibrogenesis. In the following study we described the distribution of cells that express alpha-smooth muscle actin (α-SMA) and desmin in the cat liver with various degrees of fibrosis, as well as the significance of hepatic stellate cells and portal myofibroblasts in the genesis of fibrosis in cats with polycistic kidney disease. Liver samples from 15 necropsied Persian cats were examined microscopically, using H&E and Masson-trichrom methods and immunohistology for α-SMA and desmin. Liver fibrosis was confirmed in cats with terminal stage of chronic cholangiohepatitis and it was characterized by connective tissue septa which divide the liver parenchyma into irregular lobuli. Inflammation in the cat liver is connected with the activation of periductal myofibroblasts. The intensity of immunopositivity of perisinusoidal HSCs to α-SMA and desmin varied depending on the degree of fibrosis and was the strongest in livers of cats with cirrhosis.
AB  - U ovom radu je opisana distribucija ćelija koje eksprimiraju α-glatkomišićni aktin (α-SMA) i dezmin u jetri mačaka sa različitim stadijumom fibroze, kao i značaj ovih ćelija u razvoju fibroze, kod mačaka sa policističnim bubrezima. Mikroskopski su ispitani uzorci jetre od 15 obdukovanih persijskih mačaka, korišćenjem HE i Mason-trihromnog bojenja i imunohistohemijski na α-SMA i dezmin. Fibroza jetre potvrđena je kod mačaka sa terminalnim stadijumom hroničnog holangiohepatitisa i odlikovala se prisustvom vezivno-tkivnih septi koje dele parenhim na nejednake lobuluse. Inflamacija u jetri mačaka u vezi je sa aktivacijom periduktalnih miofibroblasta. Intenzitet imunohistohemijske reakcije perisinusoidnih HSCs na α-SMA i dezmin razlikovala se od stepena fibroze i bio je najjači u jetri mačaka sa cirozom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD)
T1  - Uloga hepatičnih zvezdastih ćelija (HSCs) u razvoju fibroze jetre mačaka sa policističnim bubrezima (PKD)
VL  - 60
IS  - 4
SP  - 391
EP  - 400
DO  - 10.2298/AVB1004391A
ER  - 

@article{
author = "Aleksić-Kovačević, Sanja and Kukolj, Vladimir and Kureljušić, Branislav and Marinković, Darko and Knežević, Đ. and Ignjatović, I. and Jovanović, M. and Knežević, Milijana and Gledić, Dušan",
year = "2010",
abstract = "Hepatic stellate cells (HSCs) play a significant role in hepatic fibrogenesis. In the following study we described the distribution of cells that express alpha-smooth muscle actin (α-SMA) and desmin in the cat liver with various degrees of fibrosis, as well as the significance of hepatic stellate cells and portal myofibroblasts in the genesis of fibrosis in cats with polycistic kidney disease. Liver samples from 15 necropsied Persian cats were examined microscopically, using H&E and Masson-trichrom methods and immunohistology for α-SMA and desmin. Liver fibrosis was confirmed in cats with terminal stage of chronic cholangiohepatitis and it was characterized by connective tissue septa which divide the liver parenchyma into irregular lobuli. Inflammation in the cat liver is connected with the activation of periductal myofibroblasts. The intensity of immunopositivity of perisinusoidal HSCs to α-SMA and desmin varied depending on the degree of fibrosis and was the strongest in livers of cats with cirrhosis., U ovom radu je opisana distribucija ćelija koje eksprimiraju α-glatkomišićni aktin (α-SMA) i dezmin u jetri mačaka sa različitim stadijumom fibroze, kao i značaj ovih ćelija u razvoju fibroze, kod mačaka sa policističnim bubrezima. Mikroskopski su ispitani uzorci jetre od 15 obdukovanih persijskih mačaka, korišćenjem HE i Mason-trihromnog bojenja i imunohistohemijski na α-SMA i dezmin. Fibroza jetre potvrđena je kod mačaka sa terminalnim stadijumom hroničnog holangiohepatitisa i odlikovala se prisustvom vezivno-tkivnih septi koje dele parenhim na nejednake lobuluse. Inflamacija u jetri mačaka u vezi je sa aktivacijom periduktalnih miofibroblasta. Intenzitet imunohistohemijske reakcije perisinusoidnih HSCs na α-SMA i dezmin razlikovala se od stepena fibroze i bio je najjači u jetri mačaka sa cirozom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD), Uloga hepatičnih zvezdastih ćelija (HSCs) u razvoju fibroze jetre mačaka sa policističnim bubrezima (PKD)",
volume = "60",
number = "4",
pages = "391-400",
doi = "10.2298/AVB1004391A"
}

Aleksić-Kovačević, S., Kukolj, V., Kureljušić, B., Marinković, D., Knežević, Đ., Ignjatović, I., Jovanović, M., Knežević, M.,& Gledić, D.. (2010). Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 391-400.
https://doi.org/10.2298/AVB1004391A

Aleksić-Kovačević S, Kukolj V, Kureljušić B, Marinković D, Knežević Đ, Ignjatović I, Jovanović M, Knežević M, Gledić D. Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD). in Acta Veterinaria-Beograd. 2010;60(4):391-400.
doi:10.2298/AVB1004391A .

Aleksić-Kovačević, Sanja, Kukolj, Vladimir, Kureljušić, Branislav, Marinković, Darko, Knežević, Đ., Ignjatović, I., Jovanović, M., Knežević, Milijana, Gledić, Dušan, "Role of hepatic stellate cells (HSCs) in the development of hepatic fibrosis in cats with polycystic kidney disease (PKD)" in Acta Veterinaria-Beograd, 60, no. 4 (2010):391-400,
https://doi.org/10.2298/AVB1004391A . .

TY  - JOUR
AU  - Becskei, Zsolt
AU  - Aleksić-Kovačević, Sanja
AU  - Rusvai, Miklos
AU  - Balka, Gyula
AU  - Jakab, Csaba
AU  - Petrović, Tamaš
AU  - Knežević, Milijana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/736
AB  - The lymphatic organs of 50 pigs from a total of eight farms located at different sites in the epizootiological region of North Backa County were studied to obtain data on the prevalence of circoviral infections in Serbia. All of the pigs examined had clinical signs suggestive of postweaning multisystemic wasting syndrome (PMWS). All pigs underwent necropsy and tissue samples were taken for histopathological, immunohistochemical (IHC) and PCR analysis. The presence of porcine circovirus 2 (PCV2) was established by PCR analysis in the organs of the pigs tested. The most frequent histopathological lesions of lymphoid tissue linked with the presence of positive immunostaining for PCV2 Cap antigen confirmed the existence of PMWS in all farms tested in North Backa County. Using PCR, histopathological and IHC techniques, the presence of PMWS was proved in the Republic of Serbia. During necropsy, generalised enlargement of the lymph nodes was evident. The most common histopathological finding was lymphocyte depletion in the follicular and perifollicular areas of lymph nodes. Infiltration by macrophages was also recorded. By IHC analysis, the cytoplasm of macrophages was shown to contain a large amount of the ORF2-coded Cap antigen of PCV2. Lymphocyte depletion and large numbers of macrophages were recorded in the tonsils, spleen, intestinal lymphatic tissue, Peyers patches and ileocaecal valve. The presence of typical granulomatous lesions with multinuclear giant cells (MGCs) was also recorded in the lymphatic tissue. Cap antigen was shown to be present in macrophages and less often in lymphocytes.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome
VL  - 58
IS  - 4
SP  - 483
EP  - 498
DO  - 10.1556/AVet.58.2010.4.9
ER  - 

@article{
author = "Becskei, Zsolt and Aleksić-Kovačević, Sanja and Rusvai, Miklos and Balka, Gyula and Jakab, Csaba and Petrović, Tamaš and Knežević, Milijana",
year = "2010",
abstract = "The lymphatic organs of 50 pigs from a total of eight farms located at different sites in the epizootiological region of North Backa County were studied to obtain data on the prevalence of circoviral infections in Serbia. All of the pigs examined had clinical signs suggestive of postweaning multisystemic wasting syndrome (PMWS). All pigs underwent necropsy and tissue samples were taken for histopathological, immunohistochemical (IHC) and PCR analysis. The presence of porcine circovirus 2 (PCV2) was established by PCR analysis in the organs of the pigs tested. The most frequent histopathological lesions of lymphoid tissue linked with the presence of positive immunostaining for PCV2 Cap antigen confirmed the existence of PMWS in all farms tested in North Backa County. Using PCR, histopathological and IHC techniques, the presence of PMWS was proved in the Republic of Serbia. During necropsy, generalised enlargement of the lymph nodes was evident. The most common histopathological finding was lymphocyte depletion in the follicular and perifollicular areas of lymph nodes. Infiltration by macrophages was also recorded. By IHC analysis, the cytoplasm of macrophages was shown to contain a large amount of the ORF2-coded Cap antigen of PCV2. Lymphocyte depletion and large numbers of macrophages were recorded in the tonsils, spleen, intestinal lymphatic tissue, Peyers patches and ileocaecal valve. The presence of typical granulomatous lesions with multinuclear giant cells (MGCs) was also recorded in the lymphatic tissue. Cap antigen was shown to be present in macrophages and less often in lymphocytes.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome",
volume = "58",
number = "4",
pages = "483-498",
doi = "10.1556/AVet.58.2010.4.9"
}

Becskei, Z., Aleksić-Kovačević, S., Rusvai, M., Balka, G., Jakab, C., Petrović, T.,& Knežević, M.. (2010). Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 58(4), 483-498.
https://doi.org/10.1556/AVet.58.2010.4.9

Becskei Z, Aleksić-Kovačević S, Rusvai M, Balka G, Jakab C, Petrović T, Knežević M. Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome. in Acta Veterinaria Hungarica. 2010;58(4):483-498.
doi:10.1556/AVet.58.2010.4.9 .

Becskei, Zsolt, Aleksić-Kovačević, Sanja, Rusvai, Miklos, Balka, Gyula, Jakab, Csaba, Petrović, Tamaš, Knežević, Milijana, "Distribution of porcine circovirus 2 cap antigen in the lymphoid tissue of pigs affected by postweaning multisystemic wasting syndrome" in Acta Veterinaria Hungarica, 58, no. 4 (2010):483-498,
https://doi.org/10.1556/AVet.58.2010.4.9 . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Stevanović, Jelka
AU  - Stevanov-Pavlović, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Knežević, Milijana
AU  - Mijačević, Zora
AU  - Božić, Tatjana
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/659
AB  - Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved.
AB  - Lokalna i sistemska zapaljenjska reakcija predstavljaju kompleksne procese koji uključuju sintezu proteina akutne faze sa mnogobrojnim funkcijama u regulaciji samog zapaljenskog procesa. Cilj ovog rada je bio da se definiše lokalni i sistemski odgovor proteina akutne faze, koji je podstaknut prirodnom subkliničkom infekcijom mlečne žlezde sa Staph. aureus kod mlečnih krava, ali sa različitim brojem inficiranih četvrti. Mlečne krave (n=30) u sredini laktacionog perioda su podeljene u tri grupe. U prvoj grupi su bile krave sa bakteriološki negativnim uzorcima mleka (BN grupa), u drugoj krave sa jednom četvrti vimena inficiranom sa Staph. aureus (SaQ1), dok su u trećoj grupi bile krave sa dve četvrti vimena inficiranim sa Staph. aureus (SaQ2). Uzorke mleka smo analizirali na sledeće pokazatelje zapaljenja: serum amiloid A (SAA) i broj somatskih ćelija (BSĆ). U uzorcima krvnog seruma su ispitivani SAA, haptoglobin (Hp), ceruloplazmin (Cp) i koncentracija albumina. Takođe je urađena kompletna krvna slika. BSĆ i SAA su bili najniži u mleku iz BN grupe i najviši u SaQ2 grupi. U uzorcima krvnog seruma SAA, Hp, Cp i koncentracija albumina bili su značajno viši samo u SaQ2 grupi u poređenju sa BN grupom. Broj leukocita kao i koncentracija haptoglobina su bili u fiziološkim granicama kod sve tri grupe krava. Ovi rezultati ukazuju da krave koje imaju subkliničku infekciju sa Staph. aureus u dve četvrti vimena imaju i jače izraženi sistemski odgovor proteina akutne faze nego krave kojima je inficirana samo jedna četvrt vimena čime je pokazano da opseg povrede tkiva ima uticaj na koncentraciju proteina akutne faze u serumu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Acute phase protein response in cows with staphylococcus aureus subclinical mastitis
T1  - Reakcija proteina akutne faze kod krava sa subkliničkim mastitisom izazvanim staphilococcus aureus-om
VL  - 60
IS  - 2-3
SP  - 205
EP  - 216
DO  - 10.2298/AVB1003205K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Stevanović, Jelka and Stevanov-Pavlović, Marija and Debeljak-Martačić, Jasmina and Knežević, Milijana and Mijačević, Zora and Božić, Tatjana",
year = "2010",
abstract = "Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved., Lokalna i sistemska zapaljenjska reakcija predstavljaju kompleksne procese koji uključuju sintezu proteina akutne faze sa mnogobrojnim funkcijama u regulaciji samog zapaljenskog procesa. Cilj ovog rada je bio da se definiše lokalni i sistemski odgovor proteina akutne faze, koji je podstaknut prirodnom subkliničkom infekcijom mlečne žlezde sa Staph. aureus kod mlečnih krava, ali sa različitim brojem inficiranih četvrti. Mlečne krave (n=30) u sredini laktacionog perioda su podeljene u tri grupe. U prvoj grupi su bile krave sa bakteriološki negativnim uzorcima mleka (BN grupa), u drugoj krave sa jednom četvrti vimena inficiranom sa Staph. aureus (SaQ1), dok su u trećoj grupi bile krave sa dve četvrti vimena inficiranim sa Staph. aureus (SaQ2). Uzorke mleka smo analizirali na sledeće pokazatelje zapaljenja: serum amiloid A (SAA) i broj somatskih ćelija (BSĆ). U uzorcima krvnog seruma su ispitivani SAA, haptoglobin (Hp), ceruloplazmin (Cp) i koncentracija albumina. Takođe je urađena kompletna krvna slika. BSĆ i SAA su bili najniži u mleku iz BN grupe i najviši u SaQ2 grupi. U uzorcima krvnog seruma SAA, Hp, Cp i koncentracija albumina bili su značajno viši samo u SaQ2 grupi u poređenju sa BN grupom. Broj leukocita kao i koncentracija haptoglobina su bili u fiziološkim granicama kod sve tri grupe krava. Ovi rezultati ukazuju da krave koje imaju subkliničku infekciju sa Staph. aureus u dve četvrti vimena imaju i jače izraženi sistemski odgovor proteina akutne faze nego krave kojima je inficirana samo jedna četvrt vimena čime je pokazano da opseg povrede tkiva ima uticaj na koncentraciju proteina akutne faze u serumu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Acute phase protein response in cows with staphylococcus aureus subclinical mastitis, Reakcija proteina akutne faze kod krava sa subkliničkim mastitisom izazvanim staphilococcus aureus-om",
volume = "60",
number = "2-3",
pages = "205-216",
doi = "10.2298/AVB1003205K"
}

Kovačević-Filipović, M., Stevanović, J., Stevanov-Pavlović, M., Debeljak-Martačić, J., Knežević, M., Mijačević, Z.,& Božić, T.. (2010). Acute phase protein response in cows with staphylococcus aureus subclinical mastitis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 205-216.
https://doi.org/10.2298/AVB1003205K

Kovačević-Filipović M, Stevanović J, Stevanov-Pavlović M, Debeljak-Martačić J, Knežević M, Mijačević Z, Božić T. Acute phase protein response in cows with staphylococcus aureus subclinical mastitis. in Acta Veterinaria-Beograd. 2010;60(2-3):205-216.
doi:10.2298/AVB1003205K .

Kovačević-Filipović, Milica, Stevanović, Jelka, Stevanov-Pavlović, Marija, Debeljak-Martačić, Jasmina, Knežević, Milijana, Mijačević, Zora, Božić, Tatjana, "Acute phase protein response in cows with staphylococcus aureus subclinical mastitis" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):205-216,
https://doi.org/10.2298/AVB1003205K . .

TY  - JOUR
AU  - Marinković, Darko
AU  - Aleksić-Kovačević, Sanja
AU  - Krstić, Vanja
AU  - Knežević, Milijana
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/610
AB  - Cranial mesenteric arteries of 18 sacrificed necropsied horses of both sexes and different age groups were described in this paper. After macroscopic examination tissue samples for pathohistological examinations were routinely processed and stained with hematoxylin eosine (HE), Weigert van Gieson and Periodic-Acid-Schiff (PAS) staining. Immunohistochemical staining was performed on selected sections using avidin-biotin-peroxidase complex technique for ásmooth muscle actin (α-SMA). Enlarged, thickened cranial mesenteric arteries with a hardelastic consistency and narrowed lumen, were macroscopically evident in all examined sick horses. Live larvae of Strongylus vulgaris, situated free in the lumen, or attached to the intima of the blood vessel or incorporated in the thrombus were noted macroscopically in 37.5% examined sick horses. Inflammatory and fibrous changes were noticed and were present in the intima, media and adventitia of the blood vessel. The inflammatory infiltrate in the intima of the cranial mesenteric artery consisted of eosinophils, macrophages, plasma cells, lymphocytes and rare multinucleated giant cells and was situated mostly close to the Strongylus vulgaris larvae. Beside inflammatory changes, intimal fibrosis and extension, characterized by increased proliferation of α-SMA positive cells, was notable. Lesions of the internal elastic lamina consequently led to an inflammatory and fibrotic reaction in the tunica media. Fibrosis of the media characterized by the presence of connective tissue cells and fibers, as well as smoothmuscle cells, was present in 93.75% examined sick horses. Inflammation and fibrosis were mildest in the adventitia. Lesions of vasa vasorum were present in 81.25% examined sick animals were characterized by fibrosis, obliteration, perivascular cellular infiltration, mostly with eosinophils and findings of intimal bodies. All described changes are characteristic for cranial mesenteric artery verminous arteritis.
AB  - U ovom radu su ispitane kranijalne mezeneterijalne arterije 18 obdukovanih žrtvovanih konja različitog pola i starosti. Arterije su makroskopski pregledane i uzimani su tkivni isečci za patohistološka ispitivanja. Materijal za histološka ispitivanja fiksiran je u 10% puferizovanom formalinu i posle uobičajene procedure ukalupljen u parafinske blokove. Patohistološki preparati bojeni su hematoksilinom i eozinom (HE), Vajgert van Gison metodom i PAS metodom. Odabrani isečci imunohistohemijski su bojeni avidin-biotin-peroksidaza tehnikom za α-smooth muscle actin (α-SMA). Makroskopski, kranijalne mezenterijalne arterije svih ispitanih konja bile su uvećane, zadebljale, čvrsto-elastične konzistencije i suženog lumena. Žive larve Strongylus vulgaris slobodne u lumenu ili pričvršćene za intimu krvnog suda ili inkorporisane u trombnu masu uočene su makroskopski kod 37,5% obolelih konja. Mikroskopski su uočene promene inflamatornog do fibroznog karaktera i bile su zastupljene u intimi, mediji i adventiciji krvnog suda. Inflamatorni infiltrat u intimi su činili eozinofilni granulociti, makrofage, plazma ćelije, limfociti i višejedarne džinovske ćelije i ovaj infiltrat bio je prisutan uglavnom u blizini larve Strongylus vulgaris. Pored inflamatornih promena uočeno je zadebljavanje sa fibrozom intime koja se karakterisala proliferacijom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija. Lezije na unutrašnjoj elastičnoj lamini posledično su dovodile do inflamacije i fibroze tunike medije. Fibroza medije se karakterisala prisustvom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija i bila je prisutna kod 93,75% obolelih konja. Inflamacija i fibroza su najslabije bile izražene u adventiciji, a lezije vasa vasorum su bile prisutne kod 81,25% pregledanih obolelih životinja i karakterisale su se fibrozom, obliteracijom, perivaskularnom ćelijskom infiltracijom, uglavnom sa eozinofilima, kao i nalazom intimalnih tela. Sve opisane promene su karakteristične za verminozni arteritis kranijalne mezenterijalne arterije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological findings in the cranial mesenteric artery of horses with verminous arteritis
T1  - Morfološki nalaz na kranijalnim mezenterijalnim arterijama konja sa verminoznim arteritisom
VL  - 59
IS  - 2-3
SP  - 231
EP  - 241
DO  - 10.2298/AVB0903231M
ER  - 

@article{
author = "Marinković, Darko and Aleksić-Kovačević, Sanja and Krstić, Vanja and Knežević, Milijana",
year = "2009",
abstract = "Cranial mesenteric arteries of 18 sacrificed necropsied horses of both sexes and different age groups were described in this paper. After macroscopic examination tissue samples for pathohistological examinations were routinely processed and stained with hematoxylin eosine (HE), Weigert van Gieson and Periodic-Acid-Schiff (PAS) staining. Immunohistochemical staining was performed on selected sections using avidin-biotin-peroxidase complex technique for ásmooth muscle actin (α-SMA). Enlarged, thickened cranial mesenteric arteries with a hardelastic consistency and narrowed lumen, were macroscopically evident in all examined sick horses. Live larvae of Strongylus vulgaris, situated free in the lumen, or attached to the intima of the blood vessel or incorporated in the thrombus were noted macroscopically in 37.5% examined sick horses. Inflammatory and fibrous changes were noticed and were present in the intima, media and adventitia of the blood vessel. The inflammatory infiltrate in the intima of the cranial mesenteric artery consisted of eosinophils, macrophages, plasma cells, lymphocytes and rare multinucleated giant cells and was situated mostly close to the Strongylus vulgaris larvae. Beside inflammatory changes, intimal fibrosis and extension, characterized by increased proliferation of α-SMA positive cells, was notable. Lesions of the internal elastic lamina consequently led to an inflammatory and fibrotic reaction in the tunica media. Fibrosis of the media characterized by the presence of connective tissue cells and fibers, as well as smoothmuscle cells, was present in 93.75% examined sick horses. Inflammation and fibrosis were mildest in the adventitia. Lesions of vasa vasorum were present in 81.25% examined sick animals were characterized by fibrosis, obliteration, perivascular cellular infiltration, mostly with eosinophils and findings of intimal bodies. All described changes are characteristic for cranial mesenteric artery verminous arteritis., U ovom radu su ispitane kranijalne mezeneterijalne arterije 18 obdukovanih žrtvovanih konja različitog pola i starosti. Arterije su makroskopski pregledane i uzimani su tkivni isečci za patohistološka ispitivanja. Materijal za histološka ispitivanja fiksiran je u 10% puferizovanom formalinu i posle uobičajene procedure ukalupljen u parafinske blokove. Patohistološki preparati bojeni su hematoksilinom i eozinom (HE), Vajgert van Gison metodom i PAS metodom. Odabrani isečci imunohistohemijski su bojeni avidin-biotin-peroksidaza tehnikom za α-smooth muscle actin (α-SMA). Makroskopski, kranijalne mezenterijalne arterije svih ispitanih konja bile su uvećane, zadebljale, čvrsto-elastične konzistencije i suženog lumena. Žive larve Strongylus vulgaris slobodne u lumenu ili pričvršćene za intimu krvnog suda ili inkorporisane u trombnu masu uočene su makroskopski kod 37,5% obolelih konja. Mikroskopski su uočene promene inflamatornog do fibroznog karaktera i bile su zastupljene u intimi, mediji i adventiciji krvnog suda. Inflamatorni infiltrat u intimi su činili eozinofilni granulociti, makrofage, plazma ćelije, limfociti i višejedarne džinovske ćelije i ovaj infiltrat bio je prisutan uglavnom u blizini larve Strongylus vulgaris. Pored inflamatornih promena uočeno je zadebljavanje sa fibrozom intime koja se karakterisala proliferacijom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija. Lezije na unutrašnjoj elastičnoj lamini posledično su dovodile do inflamacije i fibroze tunike medije. Fibroza medije se karakterisala prisustvom vezivno-tkivnih ćelija i vlakana, kao i glatko-mišićnih ćelija i bila je prisutna kod 93,75% obolelih konja. Inflamacija i fibroza su najslabije bile izražene u adventiciji, a lezije vasa vasorum su bile prisutne kod 81,25% pregledanih obolelih životinja i karakterisale su se fibrozom, obliteracijom, perivaskularnom ćelijskom infiltracijom, uglavnom sa eozinofilima, kao i nalazom intimalnih tela. Sve opisane promene su karakteristične za verminozni arteritis kranijalne mezenterijalne arterije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological findings in the cranial mesenteric artery of horses with verminous arteritis, Morfološki nalaz na kranijalnim mezenterijalnim arterijama konja sa verminoznim arteritisom",
volume = "59",
number = "2-3",
pages = "231-241",
doi = "10.2298/AVB0903231M"
}

Marinković, D., Aleksić-Kovačević, S., Krstić, V.,& Knežević, M.. (2009). Morphological findings in the cranial mesenteric artery of horses with verminous arteritis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 231-241.
https://doi.org/10.2298/AVB0903231M

Marinković D, Aleksić-Kovačević S, Krstić V, Knežević M. Morphological findings in the cranial mesenteric artery of horses with verminous arteritis. in Acta Veterinaria-Beograd. 2009;59(2-3):231-241.
doi:10.2298/AVB0903231M .

Marinković, Darko, Aleksić-Kovačević, Sanja, Krstić, Vanja, Knežević, Milijana, "Morphological findings in the cranial mesenteric artery of horses with verminous arteritis" in Acta Veterinaria-Beograd, 59, no. 2-3 (2009):231-241,
https://doi.org/10.2298/AVB0903231M . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Aleksić-Kovačević, Sanja
AU  - Knežević, Milijana
AU  - Dimitrijević, Sanda
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/639
AB  - In order to detect and establish the expression of the CD57 lymphocyte population in the caecum and caecal tonsils in broilers, immunohistochemical studies of tissue samples of broilers experimentally infected with E. tenella at 21 days of age were carried out. Immunohistochemical investigations were performed with the aid of the avidin-biotin technique (LSAB), by using mouse monoclonal anti- CD57 antibodies. Increased immunoreactivity of the lamina epithelialis, mucosal lamina propria and caecal submucosa was detected as early as two days after infection, increasing at its highest at the end of the fourth day after infection. On the fifth, sixth and seventh day after infection the degree of expression of CD57 lymphocytes in the caecal mucosa of experimental birds decreases. The immunoreactivity of the tissue of the stroma of the mucosal and submucosal lamina propria of the caecal tonsils was most intensive the fourth, sixth and seventh day after infection. A discrete immunoreactivity decrease was present during the fifth day after infection. The so established CD57 lymphocyte population activity in the caecum and caecal tonsils substantiates the existence of NK cytotoxic activity of intraepithelial lymphocytes, as well as the role of these cells in the intestinal mucosa defense mechanisms against intracellular microorganisms.
AB  - U cilju detekcije i utvrđivanja ekspresije CD57 populacije limfocita u cekumu i cekalnim tonzilama pilića, izvedeno je imunohistohemijsko ispitivanje tkivnih isečaka kod jedinki koje su 21. dana starosti eksperimentalno inficirane protozoom E. tenella. Ova ispitivanja su izvedena avidin-biotin tehnikom (LSAB), korišćenjem monoklonskih mišijih anti-CD57 antitela. Pojačana imunoreaktivnost laminae epithelialis, laminae propriae mukoze i submukoze cekuma zapažena je već drugog dana posle infekcije, sa najvećim intenzitetom krajem četvrtog dana. Petog, šestog i sedmog dana posle infekcije, stepen ekspresije CD57 limfocita u mukozi cekuma eksperimentalnih životinja se smanjivao. Imunoreaktivnost tkiva cekalnih tonzila najintenzivnija je četvrtog, šestog i sedmog dana posle infekcije, u stromi laminae propriae mukoze i submukozi, uz postojanje blagog pada u petom danu posle infekcije. Dokazana imunoreaktivnost CD57 populacije limfocita u cekumima i cekalnim tonzilama, potvrđuje NK ćelijsku citotoksičnu aktivnost populacije intraepitelnih limfocita i ukazuje na važnu ulogu ovih ćelija u organizaciji odbrane crevne mukoze protiv intracelularnih mikroorganizama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - CD 57 expression on lymphocytes present in the caecum and caecal tonsils in broilers infected with Eimeria tenella
T1  - Ekspresija CD57 na limfocitima cekuma i cekalnih tonzila brojlera inficiranih sa Eimeria tenella
VL  - 59
IS  - 4
SP  - 371
EP  - 380
DO  - 10.2298/AVB0904371I
ER  - 

@article{
author = "Ilić, Tamara and Aleksić-Kovačević, Sanja and Knežević, Milijana and Dimitrijević, Sanda",
year = "2009",
abstract = "In order to detect and establish the expression of the CD57 lymphocyte population in the caecum and caecal tonsils in broilers, immunohistochemical studies of tissue samples of broilers experimentally infected with E. tenella at 21 days of age were carried out. Immunohistochemical investigations were performed with the aid of the avidin-biotin technique (LSAB), by using mouse monoclonal anti- CD57 antibodies. Increased immunoreactivity of the lamina epithelialis, mucosal lamina propria and caecal submucosa was detected as early as two days after infection, increasing at its highest at the end of the fourth day after infection. On the fifth, sixth and seventh day after infection the degree of expression of CD57 lymphocytes in the caecal mucosa of experimental birds decreases. The immunoreactivity of the tissue of the stroma of the mucosal and submucosal lamina propria of the caecal tonsils was most intensive the fourth, sixth and seventh day after infection. A discrete immunoreactivity decrease was present during the fifth day after infection. The so established CD57 lymphocyte population activity in the caecum and caecal tonsils substantiates the existence of NK cytotoxic activity of intraepithelial lymphocytes, as well as the role of these cells in the intestinal mucosa defense mechanisms against intracellular microorganisms., U cilju detekcije i utvrđivanja ekspresije CD57 populacije limfocita u cekumu i cekalnim tonzilama pilića, izvedeno je imunohistohemijsko ispitivanje tkivnih isečaka kod jedinki koje su 21. dana starosti eksperimentalno inficirane protozoom E. tenella. Ova ispitivanja su izvedena avidin-biotin tehnikom (LSAB), korišćenjem monoklonskih mišijih anti-CD57 antitela. Pojačana imunoreaktivnost laminae epithelialis, laminae propriae mukoze i submukoze cekuma zapažena je već drugog dana posle infekcije, sa najvećim intenzitetom krajem četvrtog dana. Petog, šestog i sedmog dana posle infekcije, stepen ekspresije CD57 limfocita u mukozi cekuma eksperimentalnih životinja se smanjivao. Imunoreaktivnost tkiva cekalnih tonzila najintenzivnija je četvrtog, šestog i sedmog dana posle infekcije, u stromi laminae propriae mukoze i submukozi, uz postojanje blagog pada u petom danu posle infekcije. Dokazana imunoreaktivnost CD57 populacije limfocita u cekumima i cekalnim tonzilama, potvrđuje NK ćelijsku citotoksičnu aktivnost populacije intraepitelnih limfocita i ukazuje na važnu ulogu ovih ćelija u organizaciji odbrane crevne mukoze protiv intracelularnih mikroorganizama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "CD 57 expression on lymphocytes present in the caecum and caecal tonsils in broilers infected with Eimeria tenella, Ekspresija CD57 na limfocitima cekuma i cekalnih tonzila brojlera inficiranih sa Eimeria tenella",
volume = "59",
number = "4",
pages = "371-380",
doi = "10.2298/AVB0904371I"
}

Ilić, T., Aleksić-Kovačević, S., Knežević, M.,& Dimitrijević, S.. (2009). CD 57 expression on lymphocytes present in the caecum and caecal tonsils in broilers infected with Eimeria tenella. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 371-380.
https://doi.org/10.2298/AVB0904371I

Ilić T, Aleksić-Kovačević S, Knežević M, Dimitrijević S. CD 57 expression on lymphocytes present in the caecum and caecal tonsils in broilers infected with Eimeria tenella. in Acta Veterinaria-Beograd. 2009;59(4):371-380.
doi:10.2298/AVB0904371I .

Ilić, Tamara, Aleksić-Kovačević, Sanja, Knežević, Milijana, Dimitrijević, Sanda, "CD 57 expression on lymphocytes present in the caecum and caecal tonsils in broilers infected with Eimeria tenella" in Acta Veterinaria-Beograd, 59, no. 4 (2009):371-380,
https://doi.org/10.2298/AVB0904371I . .

TY  - JOUR
AU  - Pejović, N.
AU  - Velhner, Maja
AU  - Polaček, Vladimir
AU  - Aleksić-Kovačević, Sanja
AU  - Marinković, Darko
AU  - Knežević, Milijana
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/478
AB  - In this paper are described the macroscopic, microscopic and immunophenotypic characteristics of tumors in Marek’s disease (MD). Liver, kidneys, lungs, heart, proventriclus, ovary and nerves of sciatic plexus collected from 20 chickens aged 8 to 24 weeks were examined. Lymphoproliferative enlargement in various organs of focal and diffuse character was found. The affected organs were enlarged, compact and very brittle, irregular in shape, grayish-red or grayish-white and of fatty consistency. Most often characteristic changes were in the spleen, liver, proventriculus and ovary that usually had a compact or ribbed, fatty cauliflower like formation, different in size. On histological examination proliferation of small and medium size lymphocytes, lymphoblast cells, Marek’s disease cells and activated reticulum cells were found. Tumor proliferates predominantly consisted of lymphoblasts and had all the characteristics of a lymphoma. Immunohistochemical analysis revealed that lymphoma cells in MD are of CD3 + phenotype. In liver proliferates numerous CD79+ cells were found perivasculary, which is an interesting finding demanding further research.
AB  - U ovom radu su opisane makroskopske, mikroskopske i imunofenotipske karakteristike tumora kod Marekove bolesti (MB). Ispitivanjem su bili obuhvaćeni jetra, bubreg, pluća, srce, žlezdani želudac, jajnik i nervi ishijadičnog pleksusa dvadeset pilića starosti između8i24 nedelje. U ispitivanom materijalu ustanovljeno je limfoproliferativno bujanje u različitim organima, fokalnog ili difuznog karaktera, pri čemu su zahvaćeni organi znatno povećani, kompaktni i veoma krti, često nepravilnog oblika, sivocrveni ili sivobeli, slaninastog izgleda. Najčešće i najkarakterističnije su bili promenjeni slezina, jetra, žlezdani želudac, jajnici koji obično predstavljaju kompaktne ili izbrazdane tvorevine poput karfiola, slaninastog izgleda i različite veličine. Histološki, u organima se nalaze proliferati malih i srednjih limfocita, zatim limfoblasta, ćelija Marekove bolesti i aktivisane retikulumske ćelije. Tumorski proliferati su predominantno sastavljeni od limfoblasta i imaju sve karakteristike limfoma. Imunohistohemijskom analizom je ustanovljeno da su ćelije limfoma kod MB predominantno T ćelijskog tipa, CD3 fenotipa. U proliferatu u jetri su nađene i brojne CD79 pozitivne ćelije lokalizovane perivaskularno, što predstavlja interesantan nalaz i zahteva dalja ispitivanja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological and immunohistochemical examination of tumor cells in Marek’s disease
T1  - Morfološko i imunocitohemijsko ispitivanje tumorskih ćelija kod Marekove bolesti
VL  - 57
IS  - 1
SP  - 27
EP  - 35
DO  - 10.2298/AVB0701027P
ER  - 

@article{
author = "Pejović, N. and Velhner, Maja and Polaček, Vladimir and Aleksić-Kovačević, Sanja and Marinković, Darko and Knežević, Milijana",
year = "2007",
abstract = "In this paper are described the macroscopic, microscopic and immunophenotypic characteristics of tumors in Marek’s disease (MD). Liver, kidneys, lungs, heart, proventriclus, ovary and nerves of sciatic plexus collected from 20 chickens aged 8 to 24 weeks were examined. Lymphoproliferative enlargement in various organs of focal and diffuse character was found. The affected organs were enlarged, compact and very brittle, irregular in shape, grayish-red or grayish-white and of fatty consistency. Most often characteristic changes were in the spleen, liver, proventriculus and ovary that usually had a compact or ribbed, fatty cauliflower like formation, different in size. On histological examination proliferation of small and medium size lymphocytes, lymphoblast cells, Marek’s disease cells and activated reticulum cells were found. Tumor proliferates predominantly consisted of lymphoblasts and had all the characteristics of a lymphoma. Immunohistochemical analysis revealed that lymphoma cells in MD are of CD3 + phenotype. In liver proliferates numerous CD79+ cells were found perivasculary, which is an interesting finding demanding further research., U ovom radu su opisane makroskopske, mikroskopske i imunofenotipske karakteristike tumora kod Marekove bolesti (MB). Ispitivanjem su bili obuhvaćeni jetra, bubreg, pluća, srce, žlezdani želudac, jajnik i nervi ishijadičnog pleksusa dvadeset pilića starosti između8i24 nedelje. U ispitivanom materijalu ustanovljeno je limfoproliferativno bujanje u različitim organima, fokalnog ili difuznog karaktera, pri čemu su zahvaćeni organi znatno povećani, kompaktni i veoma krti, često nepravilnog oblika, sivocrveni ili sivobeli, slaninastog izgleda. Najčešće i najkarakterističnije su bili promenjeni slezina, jetra, žlezdani želudac, jajnici koji obično predstavljaju kompaktne ili izbrazdane tvorevine poput karfiola, slaninastog izgleda i različite veličine. Histološki, u organima se nalaze proliferati malih i srednjih limfocita, zatim limfoblasta, ćelija Marekove bolesti i aktivisane retikulumske ćelije. Tumorski proliferati su predominantno sastavljeni od limfoblasta i imaju sve karakteristike limfoma. Imunohistohemijskom analizom je ustanovljeno da su ćelije limfoma kod MB predominantno T ćelijskog tipa, CD3 fenotipa. U proliferatu u jetri su nađene i brojne CD79 pozitivne ćelije lokalizovane perivaskularno, što predstavlja interesantan nalaz i zahteva dalja ispitivanja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological and immunohistochemical examination of tumor cells in Marek’s disease, Morfološko i imunocitohemijsko ispitivanje tumorskih ćelija kod Marekove bolesti",
volume = "57",
number = "1",
pages = "27-35",
doi = "10.2298/AVB0701027P"
}

Pejović, N., Velhner, M., Polaček, V., Aleksić-Kovačević, S., Marinković, D.,& Knežević, M.. (2007). Morphological and immunohistochemical examination of tumor cells in Marek’s disease. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(1), 27-35.
https://doi.org/10.2298/AVB0701027P

Pejović N, Velhner M, Polaček V, Aleksić-Kovačević S, Marinković D, Knežević M. Morphological and immunohistochemical examination of tumor cells in Marek’s disease. in Acta Veterinaria-Beograd. 2007;57(1):27-35.
doi:10.2298/AVB0701027P .

Pejović, N., Velhner, Maja, Polaček, Vladimir, Aleksić-Kovačević, Sanja, Marinković, Darko, Knežević, Milijana, "Morphological and immunohistochemical examination of tumor cells in Marek’s disease" in Acta Veterinaria-Beograd, 57, no. 1 (2007):27-35,
https://doi.org/10.2298/AVB0701027P . .

TY  - CHAP
AU  - Knežević, Milijana
AU  - Aleksić-Kovačević, Sanja
AU  - Aleksić, Zoran
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/442
AB  - Esophagogastric ulcer is an independent disease in swine that is characterized by ulcerous autodigestion of the cutaneous mucosa, which does not exhibit a tendency to recover, but, on the contrary, a tendency toward severe hemorrhaging, with a predominantly lethal outcome. Since it develops in the part of the stomach that is morphologically and functionally different from other glandular mucosa, it was questioned earlier whether it could be a peptic ulcer based on its nature. Spontaneous ulcers, usually of the stomach, commonly occur in many domestic animals. Some of these lesions are chronic and they may occur in either the glandular or squamous-lined regions of the stomach. As with the human disease, the pathogenesis in domestic animals is multifactorial, poorly understood, and variable between and within species. Environmental stress and dietary factors are very important in the ulcer disease in swine. It has been shown that the Helicobacter spp. is strongly associated with naturally occurring ulcer and preulcer lesions of the pars esophagea in swine, which raises the possibility that Helicobacter spp. is an important factor in the pathogenesis of these lesions. The dynamics of the development of esophagogastric ulcers imply hyperplastic lesions (parakeratosis and hyperkeratosis), keratolysis, erosions, peptic necrosis, and the development of ulcers with all the characteristics of peptic ulcerations in other localities. In addition, K6 is expressed in association with the mucosal changes. The pattern of the intermediate filaments of keratin suggests that epithelial proliferation, which leads to visible hyperkeratosis, constitutes the essence of gastric ulcers in swine.
PB  - Elsevier Academic Press Inc, San Diego
T2  - International Review of Cytology - A Survey of Cell Biology, Vol 260
T1  - Cell proliferation in pathogenesis of esophagogastric lesions in pigs
VL  - 260
SP  - 1
EP  - 34
DO  - 10.1016/S0074-7696(06)60001-6
ER  - 

@inbook{
author = "Knežević, Milijana and Aleksić-Kovačević, Sanja and Aleksić, Zoran",
year = "2007",
abstract = "Esophagogastric ulcer is an independent disease in swine that is characterized by ulcerous autodigestion of the cutaneous mucosa, which does not exhibit a tendency to recover, but, on the contrary, a tendency toward severe hemorrhaging, with a predominantly lethal outcome. Since it develops in the part of the stomach that is morphologically and functionally different from other glandular mucosa, it was questioned earlier whether it could be a peptic ulcer based on its nature. Spontaneous ulcers, usually of the stomach, commonly occur in many domestic animals. Some of these lesions are chronic and they may occur in either the glandular or squamous-lined regions of the stomach. As with the human disease, the pathogenesis in domestic animals is multifactorial, poorly understood, and variable between and within species. Environmental stress and dietary factors are very important in the ulcer disease in swine. It has been shown that the Helicobacter spp. is strongly associated with naturally occurring ulcer and preulcer lesions of the pars esophagea in swine, which raises the possibility that Helicobacter spp. is an important factor in the pathogenesis of these lesions. The dynamics of the development of esophagogastric ulcers imply hyperplastic lesions (parakeratosis and hyperkeratosis), keratolysis, erosions, peptic necrosis, and the development of ulcers with all the characteristics of peptic ulcerations in other localities. In addition, K6 is expressed in association with the mucosal changes. The pattern of the intermediate filaments of keratin suggests that epithelial proliferation, which leads to visible hyperkeratosis, constitutes the essence of gastric ulcers in swine.",
publisher = "Elsevier Academic Press Inc, San Diego",
journal = "International Review of Cytology - A Survey of Cell Biology, Vol 260",
booktitle = "Cell proliferation in pathogenesis of esophagogastric lesions in pigs",
volume = "260",
pages = "1-34",
doi = "10.1016/S0074-7696(06)60001-6"
}

Knežević, M., Aleksić-Kovačević, S.,& Aleksić, Z.. (2007). Cell proliferation in pathogenesis of esophagogastric lesions in pigs. in International Review of Cytology - A Survey of Cell Biology, Vol 260
Elsevier Academic Press Inc, San Diego., 260, 1-34.
https://doi.org/10.1016/S0074-7696(06)60001-6

Knežević M, Aleksić-Kovačević S, Aleksić Z. Cell proliferation in pathogenesis of esophagogastric lesions in pigs. in International Review of Cytology - A Survey of Cell Biology, Vol 260. 2007;260:1-34.
doi:10.1016/S0074-7696(06)60001-6 .

Knežević, Milijana, Aleksić-Kovačević, Sanja, Aleksić, Zoran, "Cell proliferation in pathogenesis of esophagogastric lesions in pigs" in International Review of Cytology - A Survey of Cell Biology, Vol 260, 260 (2007):1-34,
https://doi.org/10.1016/S0074-7696(06)60001-6 . .

TY  - JOUR
AU  - Aleksić-Kovačević, Sanja
AU  - Kukolj, Vladimir
AU  - Marinković, Darko
AU  - Knežević, Milijana
PY  - 2005
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/319
AB  - Out of the total number of canine tumors examined at the Department of pathology based on biopsy and section materials over the last five years skin tumors and mammary gland tumors were proved to be the two largest groups diagnosed in 33.4% and 36% of cases, respectively. Cutaneous tumors were diagnosed in 211 cases, i.e., in 123 (58.3%) male dogs and 88 (41.7%) bitches, of different breeds, on average 7 years old. Among the total number of 211 skin tumors, 32 types of cutaneous neoplasms were diagnosed, with epithelial and melanocytic tumors being the most predominant, followed by hematopoietic tumors and mesenchymal tumors of the skin and soft tissues. In our bioptic and section materials, epithelial and melanocytic skin tumors were the most frequent -123 cases (58.75%) with four subgroups within the group tumors with adnexal differentiation - 66 cases (53.23% of all epithelial and melanocytic skin tumors), epidermal tumors - 25 cases (20.16% of all epithelial and melanocytic skin tumors), tumors without squamous or adnexal differentiation -16 cases (12.90% of all epithelial and melanocytic skin tumors) and finally melanocytic tumors -14 cases (11.29% of all epithelial and melanocytic skin tumors). The age of dogs with epithelial and melanocytic cutaneous tumors ranged between 5 months to 15 years. The fact that the number of skin tumors diagnosed in 1999 was doubled in 2002 is of particular importance, which may be, the result of increasingly high interest of clinicians for biopsy-based diagnosis.
AB  - Od ukupnog broja tumora pasa dijagnostikovanih iz biopsijskog i sekcionog materijala, na Katedri za patologiju u poslednjih pet godina, najčešći su bili tumori kože i tumori mlečne žlezde - u 33.4% odnosno 36 % slučajeva. Tumori kože dijagnostikovani su kod 211 pasa -123 mužjaka (58,3%) i 88 ženki (41,7%), različitih rasa i prosečne starosti oko 7 godina.Od ukupnog broja tumora kože dijagnostikovana su 32 tipa kutanih neoplazmi, sa dominantno prisutnim epitelnim i melanocitnim tumorima, zatim slede intradermalne hematopoezne neoplazme i mezenhimski tumori kože i mekih tkiva. U našem biopsijskom i sekcionom materijalu u okviru najbrojnijih, epitelnih i melanocitnih tumora dijagnostikovanih u 123 (53,23%) slučaja, nalaze se četiri grupe: tumori sa adneksalnom diferencijacijom u 66 slučajeva (53,23%) epidermalni tumori - 25 slučajeva (20,16%), tumori sa skvamoznom i adneksalnom diferencijacijom 16 slučajeva (12,90%) i konačno melanocitni tumori u 14 slučajeva (11,29%) svih epitelnih i melanocitnih tumora. Starost pasa sa epitelnim i melancitnim tumorima je u intervalu od 5 meseci do 15 godina. Činjenica da je broj tumora kože dijagnostikovanih u 1999. godini udvostručen u u 2002., od posebnog je značaja, a između ostalog može ukazivati i na sve veće interesovanje kliničara za biopsijske dijagnoze.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Retrospective study of canine epithelial and melanocytic tumors
T1  - Retrospektivna analiza epitelnih i melanocitnih tumora kože pasa
VL  - 55
IS  - 4
SP  - 319
EP  - 326
DO  - 10.2298/AVB0504319A
ER  - 

@article{
author = "Aleksić-Kovačević, Sanja and Kukolj, Vladimir and Marinković, Darko and Knežević, Milijana",
year = "2005",
abstract = "Out of the total number of canine tumors examined at the Department of pathology based on biopsy and section materials over the last five years skin tumors and mammary gland tumors were proved to be the two largest groups diagnosed in 33.4% and 36% of cases, respectively. Cutaneous tumors were diagnosed in 211 cases, i.e., in 123 (58.3%) male dogs and 88 (41.7%) bitches, of different breeds, on average 7 years old. Among the total number of 211 skin tumors, 32 types of cutaneous neoplasms were diagnosed, with epithelial and melanocytic tumors being the most predominant, followed by hematopoietic tumors and mesenchymal tumors of the skin and soft tissues. In our bioptic and section materials, epithelial and melanocytic skin tumors were the most frequent -123 cases (58.75%) with four subgroups within the group tumors with adnexal differentiation - 66 cases (53.23% of all epithelial and melanocytic skin tumors), epidermal tumors - 25 cases (20.16% of all epithelial and melanocytic skin tumors), tumors without squamous or adnexal differentiation -16 cases (12.90% of all epithelial and melanocytic skin tumors) and finally melanocytic tumors -14 cases (11.29% of all epithelial and melanocytic skin tumors). The age of dogs with epithelial and melanocytic cutaneous tumors ranged between 5 months to 15 years. The fact that the number of skin tumors diagnosed in 1999 was doubled in 2002 is of particular importance, which may be, the result of increasingly high interest of clinicians for biopsy-based diagnosis., Od ukupnog broja tumora pasa dijagnostikovanih iz biopsijskog i sekcionog materijala, na Katedri za patologiju u poslednjih pet godina, najčešći su bili tumori kože i tumori mlečne žlezde - u 33.4% odnosno 36 % slučajeva. Tumori kože dijagnostikovani su kod 211 pasa -123 mužjaka (58,3%) i 88 ženki (41,7%), različitih rasa i prosečne starosti oko 7 godina.Od ukupnog broja tumora kože dijagnostikovana su 32 tipa kutanih neoplazmi, sa dominantno prisutnim epitelnim i melanocitnim tumorima, zatim slede intradermalne hematopoezne neoplazme i mezenhimski tumori kože i mekih tkiva. U našem biopsijskom i sekcionom materijalu u okviru najbrojnijih, epitelnih i melanocitnih tumora dijagnostikovanih u 123 (53,23%) slučaja, nalaze se četiri grupe: tumori sa adneksalnom diferencijacijom u 66 slučajeva (53,23%) epidermalni tumori - 25 slučajeva (20,16%), tumori sa skvamoznom i adneksalnom diferencijacijom 16 slučajeva (12,90%) i konačno melanocitni tumori u 14 slučajeva (11,29%) svih epitelnih i melanocitnih tumora. Starost pasa sa epitelnim i melancitnim tumorima je u intervalu od 5 meseci do 15 godina. Činjenica da je broj tumora kože dijagnostikovanih u 1999. godini udvostručen u u 2002., od posebnog je značaja, a između ostalog može ukazivati i na sve veće interesovanje kliničara za biopsijske dijagnoze.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Retrospective study of canine epithelial and melanocytic tumors, Retrospektivna analiza epitelnih i melanocitnih tumora kože pasa",
volume = "55",
number = "4",
pages = "319-326",
doi = "10.2298/AVB0504319A"
}

Aleksić-Kovačević, S., Kukolj, V., Marinković, D.,& Knežević, M.. (2005). Retrospective study of canine epithelial and melanocytic tumors. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 55(4), 319-326.
https://doi.org/10.2298/AVB0504319A

Aleksić-Kovačević S, Kukolj V, Marinković D, Knežević M. Retrospective study of canine epithelial and melanocytic tumors. in Acta Veterinaria-Beograd. 2005;55(4):319-326.
doi:10.2298/AVB0504319A .

Aleksić-Kovačević, Sanja, Kukolj, Vladimir, Marinković, Darko, Knežević, Milijana, "Retrospective study of canine epithelial and melanocytic tumors" in Acta Veterinaria-Beograd, 55, no. 4 (2005):319-326,
https://doi.org/10.2298/AVB0504319A . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Knežević, Milijana
AU  - Aleksić-Kovačević, Sanja
AU  - Nešić, Vladimir
AU  - Dimitrijević, Sanda
PY  - 2004
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/273
AB  - The aim of this investigation was to detect and examine the distribution of the CD3-T lymphocyte cell population of the Bursa Fabricius in chickens experimentally infected with Eimeria tenella. Slices of archived samples of Bursa were examined using the of direct peroxidase (DP) method. CD3-T lymphocytes were detected with human anti-CD3 antibody. They were localized in the cortico-medullary layer of Bursa follicles in uninfected chickens. The immunoreactivity of bursal tissue was directly correlated with the stage of development of E. tenella, with the highest intensity in the epithelium of the folds and in the layers of follicles between 3 and 5 days after the infection, predominantly in the cortico-medullary layer of the follicles. These results demonstrate the existence of an early cellular immunological response to infection with E. tenella.
AB  - Ispitivanja u ovom radu su imala za cilj detekciju i utvrđivanje distribucije CD3-T limfocitne populacije u Fabricijusovoj burzi pilića veštački inficiranih sa E. tenella. Za izvođenje imunohistohemijskih ispitivanja korišćeni su parafinski isečci burzi, koji su nakon standardne procedure obrađeni metodom direktne peroksidaze (DP). CD3-T limfocitna populacija detektovana humanim CD3 antitelom, lokalizovana je u kortiko-medularnom sloju folikula burze, kod neinficiranih pilića. Imunoreaktivnost tkiva burze u direktnoj je zavisnosti od stadijuma razvoja E. tenella, a najintenzivnija je u epitelu plike i slojevima folikula između 3. i 5. dana posle infekcije, sa dominacijom u kortiko-medularnom sloju folikula. Rezultati dobijeni u okviru navedenih ispitivanja dokazuju postojanje ranog imunološkog odgovora na infekciju prouzrokovanu sa E. tenella, koji je celularnog karaktera.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella
T1  - Imunohistohemijska ispitivanja fabricijusove burze pilića eksperimentalno inficiranih sa Eimeria tenella
VL  - 54
IS  - 5-6
SP  - 411
EP  - 417
DO  - 10.2298/AVB0406411I
ER  - 

@article{
author = "Ilić, Tamara and Knežević, Milijana and Aleksić-Kovačević, Sanja and Nešić, Vladimir and Dimitrijević, Sanda",
year = "2004",
abstract = "The aim of this investigation was to detect and examine the distribution of the CD3-T lymphocyte cell population of the Bursa Fabricius in chickens experimentally infected with Eimeria tenella. Slices of archived samples of Bursa were examined using the of direct peroxidase (DP) method. CD3-T lymphocytes were detected with human anti-CD3 antibody. They were localized in the cortico-medullary layer of Bursa follicles in uninfected chickens. The immunoreactivity of bursal tissue was directly correlated with the stage of development of E. tenella, with the highest intensity in the epithelium of the folds and in the layers of follicles between 3 and 5 days after the infection, predominantly in the cortico-medullary layer of the follicles. These results demonstrate the existence of an early cellular immunological response to infection with E. tenella., Ispitivanja u ovom radu su imala za cilj detekciju i utvrđivanje distribucije CD3-T limfocitne populacije u Fabricijusovoj burzi pilića veštački inficiranih sa E. tenella. Za izvođenje imunohistohemijskih ispitivanja korišćeni su parafinski isečci burzi, koji su nakon standardne procedure obrađeni metodom direktne peroksidaze (DP). CD3-T limfocitna populacija detektovana humanim CD3 antitelom, lokalizovana je u kortiko-medularnom sloju folikula burze, kod neinficiranih pilića. Imunoreaktivnost tkiva burze u direktnoj je zavisnosti od stadijuma razvoja E. tenella, a najintenzivnija je u epitelu plike i slojevima folikula između 3. i 5. dana posle infekcije, sa dominacijom u kortiko-medularnom sloju folikula. Rezultati dobijeni u okviru navedenih ispitivanja dokazuju postojanje ranog imunološkog odgovora na infekciju prouzrokovanu sa E. tenella, koji je celularnog karaktera.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella, Imunohistohemijska ispitivanja fabricijusove burze pilića eksperimentalno inficiranih sa Eimeria tenella",
volume = "54",
number = "5-6",
pages = "411-417",
doi = "10.2298/AVB0406411I"
}

Ilić, T., Knežević, M., Aleksić-Kovačević, S., Nešić, V.,& Dimitrijević, S.. (2004). Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(5-6), 411-417.
https://doi.org/10.2298/AVB0406411I

Ilić T, Knežević M, Aleksić-Kovačević S, Nešić V, Dimitrijević S. Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella. in Acta Veterinaria-Beograd. 2004;54(5-6):411-417.
doi:10.2298/AVB0406411I .

Ilić, Tamara, Knežević, Milijana, Aleksić-Kovačević, Sanja, Nešić, Vladimir, Dimitrijević, Sanda, "Immunohistochemical investigation of the Bursa of Fabricius in chickens experimentally infected by eimeria tenella" in Acta Veterinaria-Beograd, 54, no. 5-6 (2004):411-417,
https://doi.org/10.2298/AVB0406411I . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Knežević, Milijana
AU  - Dimitrijević, Sanda
AU  - Nešić, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/259
AB  - An immunohistochemical study of caecum tissue samples was performed on chickens that had been infected with E. tenella when 14 days old. Using antibodies of rabbits against T-CD3 lymphocytes (DAKO EPOS N0U 0026) and applying the direct peroxidase method, CD3 positive lymphocytes in caeca were marked. This lymphocyte subpopulation, was localized in the lamina epithelial mucosa of the caecum. Increased immunoreactivity in the lamina epithelial mucosa of the caecum and stroma of the lamina propria mucosa, was observed already on the first day after infection with E. tenella, with the greatest intensity occuring between the third and fifth day. These findings demonstrate the existence of an early immunological response to infection caused by E. tenella, that is cell mediated, which is one of its most important characteristics.
AB  - Imunohistohemijska ispitivanja tkivnih isečaka cekuma, izvedena su kod pilića koji su 14. dana starosti inficirani sa E. tenella. Korišćenjem komercijalnih kit-antitela kunića protiv T-CD3 limfocita i primenom metode direktne peroksidaze (DP), obeleženi su CD3 pozitivni limfociti u cekumima. Navedena limfocitna subpopulacija detektovana CD3 antitelom, lokalizovana je u lamina epithelialis mucosa cekuma. Pojačana imunoreaktivnost u lamina epithelialis mucosa cekuma i strome lamina propria mucosa, zapažena je već prvog dana posle infekcije, sa najvećim intenzitetom između 3. i 5. dana. Ovakvi nalazi dokazuju postojanje ranog imunološkog odgovora na infekciju prouzrokovanu sa E. tenella, koji je ćelijskog karaktera, što predstavlja jedno od njegovih najvažnijih obeležja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Study of the distribution of cd3-t lymphocytes in caeca of chickens experimentally infected with eimeria tenella
T1  - Ispitivanja distribucije CD3-T limfocita u cekumima pilića eksperimentalno inficiranih sa Eimeria tenella
VL  - 53
IS  - 5-6
SP  - 385
EP  - 392
DO  - 10.2298/AVB0306385I
ER  - 

@article{
author = "Ilić, Tamara and Knežević, Milijana and Dimitrijević, Sanda and Nešić, Vladimir and Aleksić-Kovačević, Sanja",
year = "2003",
abstract = "An immunohistochemical study of caecum tissue samples was performed on chickens that had been infected with E. tenella when 14 days old. Using antibodies of rabbits against T-CD3 lymphocytes (DAKO EPOS N0U 0026) and applying the direct peroxidase method, CD3 positive lymphocytes in caeca were marked. This lymphocyte subpopulation, was localized in the lamina epithelial mucosa of the caecum. Increased immunoreactivity in the lamina epithelial mucosa of the caecum and stroma of the lamina propria mucosa, was observed already on the first day after infection with E. tenella, with the greatest intensity occuring between the third and fifth day. These findings demonstrate the existence of an early immunological response to infection caused by E. tenella, that is cell mediated, which is one of its most important characteristics., Imunohistohemijska ispitivanja tkivnih isečaka cekuma, izvedena su kod pilića koji su 14. dana starosti inficirani sa E. tenella. Korišćenjem komercijalnih kit-antitela kunića protiv T-CD3 limfocita i primenom metode direktne peroksidaze (DP), obeleženi su CD3 pozitivni limfociti u cekumima. Navedena limfocitna subpopulacija detektovana CD3 antitelom, lokalizovana je u lamina epithelialis mucosa cekuma. Pojačana imunoreaktivnost u lamina epithelialis mucosa cekuma i strome lamina propria mucosa, zapažena je već prvog dana posle infekcije, sa najvećim intenzitetom između 3. i 5. dana. Ovakvi nalazi dokazuju postojanje ranog imunološkog odgovora na infekciju prouzrokovanu sa E. tenella, koji je ćelijskog karaktera, što predstavlja jedno od njegovih najvažnijih obeležja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Study of the distribution of cd3-t lymphocytes in caeca of chickens experimentally infected with eimeria tenella, Ispitivanja distribucije CD3-T limfocita u cekumima pilića eksperimentalno inficiranih sa Eimeria tenella",
volume = "53",
number = "5-6",
pages = "385-392",
doi = "10.2298/AVB0306385I"
}

Ilić, T., Knežević, M., Dimitrijević, S., Nešić, V.,& Aleksić-Kovačević, S.. (2003). Study of the distribution of cd3-t lymphocytes in caeca of chickens experimentally infected with eimeria tenella. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 385-392.
https://doi.org/10.2298/AVB0306385I

Ilić T, Knežević M, Dimitrijević S, Nešić V, Aleksić-Kovačević S. Study of the distribution of cd3-t lymphocytes in caeca of chickens experimentally infected with eimeria tenella. in Acta Veterinaria-Beograd. 2003;53(5-6):385-392.
doi:10.2298/AVB0306385I .

Ilić, Tamara, Knežević, Milijana, Dimitrijević, Sanda, Nešić, Vladimir, Aleksić-Kovačević, Sanja, "Study of the distribution of cd3-t lymphocytes in caeca of chickens experimentally infected with eimeria tenella" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):385-392,
https://doi.org/10.2298/AVB0306385I . .

TY  - JOUR
AU  - Nešić, Vladimir
AU  - Aleksić, Zoran
AU  - Dimitrijević, Sanda
AU  - Knežević, Milijana
AU  - Ilić, Tamara
AU  - Resanović, Radmila
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/263
AB  - In order to investigate cecal coccidiosis in broilers fed zeolite mixtures two experiments were performed. The first was carried out in vitro, where the concentration of anticoccidial medicine not adsorbed by zeolites was determined by a spectrophotometric method. In the second experiment 100 one-day old broilers were divided into two groups that were fed with mixed feed containing the anticoccidial (salinomycin-Na) with or without a zeolite additive. Two to 7 days after artificial infection with an Eimeria tenella culture, the birds were killed and pathoanatomical, pathohistological and coprological investigations carried out. The results showed that the intensity of infection was more pronounced in animals that received salinomycin-Na and zeolite in their feed than in those that received no zeolite. Both the experiment in vitro as well as the coprological investigation showed that bonding of salinomycin-Na by zeolite occurred leaving a smaller amount of anticoccidial, that was not able to provide the anticipated protective effect.
AB  - U cilju ispitivanja cekalne kokcidioze brojlera u uslovima ishrane smešama sa zeolitom izvedena su dva ogleda. Prvi ogled je izveden u "in vitro", uslovima, gde je spektrofotometrijskom metodom određivana koncentracija antikokcidijala koji nije adsorbovan od strane zeolita. Drugi ogled je izveden na 100 jednodnevnih brojlera podeljenih u dve grupe, koji su hranjeni krmnom smešom sa dodatkom antikokcidijala i zeolita u zavisnosti od ogledne grupe. Nakon veštačke infekcije kulturom E. tenella, vršena su patoanatomska, patohistološka i koprološka ispitivanja žrtvovanih jedinki od drugog do sedmog dana posle infekcije. Rezultati ispitivanja su pokazali da je intenzitet promena bio izraženiji kod jedinki ogledne grupe koje su u hrani dobijale salinomycin-Na i zeolit u odnosu na jedinke koje su dobijale samo salinomycin-Na. I rezultati "in vitro" ogleda i koprološkog ispitivanja su pokazali da je došlo do vezivanja salinomycin-Na od strane zeolita, usled čega je na raspolaganju ostala manja količina antikokcidijala, koja nije bila u stanju da pruži očekivani zaštitni efekat.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The influence of a diet of mixed feed containing zeolite on the course of cecal coccidiosis in broilers
T1  - Uticaj ishrane smešama sa zeolitom na tok cekalne kokcidioze brojlera
VL  - 53
IS  - 5-6
SP  - 377
EP  - 384
DO  - 10.2298/AVB0306377N
ER  - 

@article{
author = "Nešić, Vladimir and Aleksić, Zoran and Dimitrijević, Sanda and Knežević, Milijana and Ilić, Tamara and Resanović, Radmila",
year = "2003",
abstract = "In order to investigate cecal coccidiosis in broilers fed zeolite mixtures two experiments were performed. The first was carried out in vitro, where the concentration of anticoccidial medicine not adsorbed by zeolites was determined by a spectrophotometric method. In the second experiment 100 one-day old broilers were divided into two groups that were fed with mixed feed containing the anticoccidial (salinomycin-Na) with or without a zeolite additive. Two to 7 days after artificial infection with an Eimeria tenella culture, the birds were killed and pathoanatomical, pathohistological and coprological investigations carried out. The results showed that the intensity of infection was more pronounced in animals that received salinomycin-Na and zeolite in their feed than in those that received no zeolite. Both the experiment in vitro as well as the coprological investigation showed that bonding of salinomycin-Na by zeolite occurred leaving a smaller amount of anticoccidial, that was not able to provide the anticipated protective effect., U cilju ispitivanja cekalne kokcidioze brojlera u uslovima ishrane smešama sa zeolitom izvedena su dva ogleda. Prvi ogled je izveden u "in vitro", uslovima, gde je spektrofotometrijskom metodom određivana koncentracija antikokcidijala koji nije adsorbovan od strane zeolita. Drugi ogled je izveden na 100 jednodnevnih brojlera podeljenih u dve grupe, koji su hranjeni krmnom smešom sa dodatkom antikokcidijala i zeolita u zavisnosti od ogledne grupe. Nakon veštačke infekcije kulturom E. tenella, vršena su patoanatomska, patohistološka i koprološka ispitivanja žrtvovanih jedinki od drugog do sedmog dana posle infekcije. Rezultati ispitivanja su pokazali da je intenzitet promena bio izraženiji kod jedinki ogledne grupe koje su u hrani dobijale salinomycin-Na i zeolit u odnosu na jedinke koje su dobijale samo salinomycin-Na. I rezultati "in vitro" ogleda i koprološkog ispitivanja su pokazali da je došlo do vezivanja salinomycin-Na od strane zeolita, usled čega je na raspolaganju ostala manja količina antikokcidijala, koja nije bila u stanju da pruži očekivani zaštitni efekat.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The influence of a diet of mixed feed containing zeolite on the course of cecal coccidiosis in broilers, Uticaj ishrane smešama sa zeolitom na tok cekalne kokcidioze brojlera",
volume = "53",
number = "5-6",
pages = "377-384",
doi = "10.2298/AVB0306377N"
}

Nešić, V., Aleksić, Z., Dimitrijević, S., Knežević, M., Ilić, T.,& Resanović, R.. (2003). The influence of a diet of mixed feed containing zeolite on the course of cecal coccidiosis in broilers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 377-384.
https://doi.org/10.2298/AVB0306377N

Nešić V, Aleksić Z, Dimitrijević S, Knežević M, Ilić T, Resanović R. The influence of a diet of mixed feed containing zeolite on the course of cecal coccidiosis in broilers. in Acta Veterinaria-Beograd. 2003;53(5-6):377-384.
doi:10.2298/AVB0306377N .

Nešić, Vladimir, Aleksić, Zoran, Dimitrijević, Sanda, Knežević, Milijana, Ilić, Tamara, Resanović, Radmila, "The influence of a diet of mixed feed containing zeolite on the course of cecal coccidiosis in broilers" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):377-384,
https://doi.org/10.2298/AVB0306377N . .

TY  - JOUR
AU  - Božić, Tatjana
AU  - Stevanović, Jelka
AU  - Kovačević, Milica
AU  - Jović, Slavoljub
AU  - Lukić, Sanja
AU  - Petakov, Marijana
AU  - Borozan, Sunčica
AU  - Mijačević, Zora
AU  - Knežević, Milijana
AU  - Bulajić, Snežana
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/236
AB  - The influence of toluene exposure on some biochemical and hematological parameters was investigated in adult female Wistar rats. The animals were subjected to intraperitoneal administration of toluene diluted in propylene glycol and the diluent alone for 3, 7 and 11 consecutive days at the same time intervals. The effects of toluene and propylene glycol were evaluated biochemically by determining the plasma concentrations of total proteins albumin and ceruloplasmin (Cp) together with erythrocyte malondialdehyde (MDA), and hematological indices from peripheral blood and bone marrow. The biochemical acute phase response was manifested by an elevated Cp concentration in all experimental animals. The markedly enhanced MDA concentration and statistically significant decrease in albumin level in toluene treated rats, indicated damage, to blood vessel endothelia. Alterations of leukocytes of peripheral blood and bone marrow (BM) granulocytic-monocytic progenitor cells were typical of an inflammatory response, with stimulation of granulo-monocytopoiesis. Therefore, it can be assumed that both toluene and propylene glycol mediated sterile peritonitis and oxidative stress injury, with changes intensified by toluene action.
AB  - U ovom ogledu je ispitivan efekat toluena na biohemijske i hematološke parametre odraslih ženki Wistar pacova. Životinjama je intraperitonealno davan rastvor toluena u propilen glikolu, kao i propilen glikol, jednom dnevno u trajanju od 3, 7 i 11 dana. Efekti davanih rastvora odredjivani su biohemijskim i hematološkim ispitivanjima plazme, krvi i punktata kostne srži. U plazmi pacova određivani su koncentracije ukupnih proteina, albumina kao i koncentracija eritrocitnog malondialdehida (RBC MDA). Biohemijske promene manifestovale su se hipoalbuminemijom, porastom koncentracije ceruloplazmina i visokom koncentracijom RBC MDA. Ovi nalazi ukazuju na oštećenje endotela krvnih sudova praćeno odgovorom akutne faze. Promene u broju leukocita periferne krvi kao i povećanje broja granulocitno-monocitnih (CFU-GM) progenitora kostne srži takođe su ukazali na tipičan proinflamatorni odgovor. Naši rezultati potvrđuju da toluen izaziva oksidativni stres i sterilni akutni peritonitis. Promene izazvane slobodnim radikalima kiseonika utiču i na odgovor progenitora kostne srži, pojavu koja do sada nije bila opisana.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Toluene mediated oxidative stress and granulo-monocytopoiesis
T1  - Toluenom posredovani oksidativni stres i granulo-monocitopoeza
VL  - 53
IS  - 4
SP  - 201
EP  - 210
DO  - 10.2298/AVB0304201B
ER  - 

@article{
author = "Božić, Tatjana and Stevanović, Jelka and Kovačević, Milica and Jović, Slavoljub and Lukić, Sanja and Petakov, Marijana and Borozan, Sunčica and Mijačević, Zora and Knežević, Milijana and Bulajić, Snežana",
year = "2003",
abstract = "The influence of toluene exposure on some biochemical and hematological parameters was investigated in adult female Wistar rats. The animals were subjected to intraperitoneal administration of toluene diluted in propylene glycol and the diluent alone for 3, 7 and 11 consecutive days at the same time intervals. The effects of toluene and propylene glycol were evaluated biochemically by determining the plasma concentrations of total proteins albumin and ceruloplasmin (Cp) together with erythrocyte malondialdehyde (MDA), and hematological indices from peripheral blood and bone marrow. The biochemical acute phase response was manifested by an elevated Cp concentration in all experimental animals. The markedly enhanced MDA concentration and statistically significant decrease in albumin level in toluene treated rats, indicated damage, to blood vessel endothelia. Alterations of leukocytes of peripheral blood and bone marrow (BM) granulocytic-monocytic progenitor cells were typical of an inflammatory response, with stimulation of granulo-monocytopoiesis. Therefore, it can be assumed that both toluene and propylene glycol mediated sterile peritonitis and oxidative stress injury, with changes intensified by toluene action., U ovom ogledu je ispitivan efekat toluena na biohemijske i hematološke parametre odraslih ženki Wistar pacova. Životinjama je intraperitonealno davan rastvor toluena u propilen glikolu, kao i propilen glikol, jednom dnevno u trajanju od 3, 7 i 11 dana. Efekti davanih rastvora odredjivani su biohemijskim i hematološkim ispitivanjima plazme, krvi i punktata kostne srži. U plazmi pacova određivani su koncentracije ukupnih proteina, albumina kao i koncentracija eritrocitnog malondialdehida (RBC MDA). Biohemijske promene manifestovale su se hipoalbuminemijom, porastom koncentracije ceruloplazmina i visokom koncentracijom RBC MDA. Ovi nalazi ukazuju na oštećenje endotela krvnih sudova praćeno odgovorom akutne faze. Promene u broju leukocita periferne krvi kao i povećanje broja granulocitno-monocitnih (CFU-GM) progenitora kostne srži takođe su ukazali na tipičan proinflamatorni odgovor. Naši rezultati potvrđuju da toluen izaziva oksidativni stres i sterilni akutni peritonitis. Promene izazvane slobodnim radikalima kiseonika utiču i na odgovor progenitora kostne srži, pojavu koja do sada nije bila opisana.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Toluene mediated oxidative stress and granulo-monocytopoiesis, Toluenom posredovani oksidativni stres i granulo-monocitopoeza",
volume = "53",
number = "4",
pages = "201-210",
doi = "10.2298/AVB0304201B"
}

Božić, T., Stevanović, J., Kovačević, M., Jović, S., Lukić, S., Petakov, M., Borozan, S., Mijačević, Z., Knežević, M.,& Bulajić, S.. (2003). Toluene mediated oxidative stress and granulo-monocytopoiesis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(4), 201-210.
https://doi.org/10.2298/AVB0304201B

Božić T, Stevanović J, Kovačević M, Jović S, Lukić S, Petakov M, Borozan S, Mijačević Z, Knežević M, Bulajić S. Toluene mediated oxidative stress and granulo-monocytopoiesis. in Acta Veterinaria-Beograd. 2003;53(4):201-210.
doi:10.2298/AVB0304201B .

Božić, Tatjana, Stevanović, Jelka, Kovačević, Milica, Jović, Slavoljub, Lukić, Sanja, Petakov, Marijana, Borozan, Sunčica, Mijačević, Zora, Knežević, Milijana, Bulajić, Snežana, "Toluene mediated oxidative stress and granulo-monocytopoiesis" in Acta Veterinaria-Beograd, 53, no. 4 (2003):201-210,
https://doi.org/10.2298/AVB0304201B . .

TY  - JOUR
AU  - Ilić, Tamara
AU  - Knežević, Milijana
AU  - Aleksić-Kovačević, Sanja
AU  - Dimitrijević, Sanda
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/244
AB  - It is important to study the immunological response of chicken to infections caused by Eimeria spp. because control of this disease is a specific problem in conditions of intensive poultry production. The details of the protective mechanisms which are activated in the course of a coccidial infection have still not been satisfactorily explained, but it is known that cellmediated immunity plays a dominant role in defending the host from this agent. Intraepithelial lymphocytes (IEL) play a very important role in the local defense of intestinal mucosa from coccidial invasion. A certain number of IEL in chicken CD3 are positive and can be detected at the earliest on day 6 after hatching. Poultry IEL are mostly T-lymphocytes, most of which on their surface carry receptors for CD8 antigen, and since they are cytotoxic lymphocytes, they have an important role in resistance to secondary infection.
AB  - Izučavanje imunskog odgovora pilića na infekciju prouzrokovanu Eimeria spp značajno je zbog toga, što suzbijanje ovog oboljenja predstavlja specifičan problem u uslovima intenzivne živinarske proizvodnje. Dosadašnji rezultati u razvoju imunoprofilakse, kao i sumnja u dostupnost novih antikokcidijala utiču na intenziviranje istraživanja u oblasti suzbijanja kokcidioze imunološkim metodama. Njihovo potpunije poznavanje može da doprinese uspešnijem iskorenjivanju bolesti, koja prati ekonomski značajnu intenzivnu proizvodnju mesa i jaja živine. Detalji zaštitnih mehanizama, koji su se aktivirali u toku kokcidijalne infekcije, još uvek nisu u potpunosti objašnjeni, ali se zna da imunitet ćelijski posredovan ima dominantnu ulogu u odbrani domaćina od ovog uzročnika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Immunological response of chicken to coccidial infection
T1  - Imunski odgovor pilića na kokcidijalnu infekciju
VL  - 57
IS  - 7-8
SP  - 501
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_244
ER  - 

@article{
author = "Ilić, Tamara and Knežević, Milijana and Aleksić-Kovačević, Sanja and Dimitrijević, Sanda",
year = "2003",
abstract = "It is important to study the immunological response of chicken to infections caused by Eimeria spp. because control of this disease is a specific problem in conditions of intensive poultry production. The details of the protective mechanisms which are activated in the course of a coccidial infection have still not been satisfactorily explained, but it is known that cellmediated immunity plays a dominant role in defending the host from this agent. Intraepithelial lymphocytes (IEL) play a very important role in the local defense of intestinal mucosa from coccidial invasion. A certain number of IEL in chicken CD3 are positive and can be detected at the earliest on day 6 after hatching. Poultry IEL are mostly T-lymphocytes, most of which on their surface carry receptors for CD8 antigen, and since they are cytotoxic lymphocytes, they have an important role in resistance to secondary infection., Izučavanje imunskog odgovora pilića na infekciju prouzrokovanu Eimeria spp značajno je zbog toga, što suzbijanje ovog oboljenja predstavlja specifičan problem u uslovima intenzivne živinarske proizvodnje. Dosadašnji rezultati u razvoju imunoprofilakse, kao i sumnja u dostupnost novih antikokcidijala utiču na intenziviranje istraživanja u oblasti suzbijanja kokcidioze imunološkim metodama. Njihovo potpunije poznavanje može da doprinese uspešnijem iskorenjivanju bolesti, koja prati ekonomski značajnu intenzivnu proizvodnju mesa i jaja živine. Detalji zaštitnih mehanizama, koji su se aktivirali u toku kokcidijalne infekcije, još uvek nisu u potpunosti objašnjeni, ali se zna da imunitet ćelijski posredovan ima dominantnu ulogu u odbrani domaćina od ovog uzročnika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Immunological response of chicken to coccidial infection, Imunski odgovor pilića na kokcidijalnu infekciju",
volume = "57",
number = "7-8",
pages = "501-504",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_244"
}

Ilić, T., Knežević, M., Aleksić-Kovačević, S.,& Dimitrijević, S.. (2003). Immunological response of chicken to coccidial infection. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(7-8), 501-504.
https://hdl.handle.net/21.15107/rcub_veterinar_244

Ilić T, Knežević M, Aleksić-Kovačević S, Dimitrijević S. Immunological response of chicken to coccidial infection. in Veterinarski Glasnik. 2003;57(7-8):501-504.
https://hdl.handle.net/21.15107/rcub_veterinar_244 .

Ilić, Tamara, Knežević, Milijana, Aleksić-Kovačević, Sanja, Dimitrijević, Sanda, "Immunological response of chicken to coccidial infection" in Veterinarski Glasnik, 57, no. 7-8 (2003):501-504,
https://hdl.handle.net/21.15107/rcub_veterinar_244 .

TY  - JOUR
AU  - Šefer, Dragan
AU  - Knežević, Milijana
AU  - Sinovec, Snežana M.
AU  - Sinovec, Zlatan
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/233
AB  - The 42-day long trial was performed on a total of 48 Hybro broilers divided into four groups. After a 14 day pre-experimental period, the experimental groups were treated daily with T-2 toxin at 0.02 mg/kg BW for 7, 14 or 21 days respectively and a mixture of ethanol and physiological saline was administered to the control group of birds. Liver samples were taken after the period of toxin administration and the remaining birds from the control and experimental groups were normally fed and watered without toxin application until the end of the trial when liver samples were taken again. Histogenetic development of dystrophic and dyscyclic changes were found in the livers of the treated broilers. Morphological alterations of the liver were diverse in character, intensity and spread depending on the duration of exposure of broilers to the adverse effects of T-2 toxin, as well as the total quantity of toxin applied. The degree of the reparation varied depending on the duration of exposure to toxin, as well as the resting period. Evident reversible processes were found after short term exposure followed by a long resting period, while regeneration processes were not pronounced after chronic poisoning followed by a short resting period.
AB  - Ogled je izveden na 48 Hybro brojlera podeljenih u četiri grupe Eksperimentalne grupe su svakodnevno tretirane T-2 toksinom u količini od 0.02 mg/kg TM tokom 7, 14 ili 21 dana, dok je brojlerima kontrolne grupe aplikovana smeša etanola i fiziološkog rastvora. Uzorci jetre za patohistološka ispitivanja uzeti su nakon završetka tretmana, a preostali brojleri su hranjeni i pojeni bez aplikacije toksina do 42. dana kada su uzorci jetre ponovo uzeti. U jetri tretiranih brojlera utvrđen je histogenetski razvoj distrofičnih i discikličnih promena. Morfološke alteracije jetre razlikovale su se po karakteru, intenzitetu i raširenosti u zavisnosti od dužine ekspozicije brojlera, kao i od ukupne količine aplikovanog toksina. Stepen sanacionih procesa zavisio je od dužine ekspozicije brojlera, kao i od vremena oporavka. Reparacioni procesi su izraženi nakon kratkotrajnog delovanja T-2 toksina praćenog dugim periodom oporavka, dok se nakon hroničnog trovanja i kratkog vremena oporavka ne uočavaju regeneracioni procesi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pathomorphological alterations and degree of reparatory processes in the liver of broilers treated with t-2 toxin
T1  - Patohistološke promene i stepen sanacionih procesa u jetri brojlera tretiranih T-2 toksinom
VL  - 53
IS  - 1
SP  - 27
EP  - 34
DO  - 10.2298/AVB0301027S
ER  - 

@article{
author = "Šefer, Dragan and Knežević, Milijana and Sinovec, Snežana M. and Sinovec, Zlatan",
year = "2003",
abstract = "The 42-day long trial was performed on a total of 48 Hybro broilers divided into four groups. After a 14 day pre-experimental period, the experimental groups were treated daily with T-2 toxin at 0.02 mg/kg BW for 7, 14 or 21 days respectively and a mixture of ethanol and physiological saline was administered to the control group of birds. Liver samples were taken after the period of toxin administration and the remaining birds from the control and experimental groups were normally fed and watered without toxin application until the end of the trial when liver samples were taken again. Histogenetic development of dystrophic and dyscyclic changes were found in the livers of the treated broilers. Morphological alterations of the liver were diverse in character, intensity and spread depending on the duration of exposure of broilers to the adverse effects of T-2 toxin, as well as the total quantity of toxin applied. The degree of the reparation varied depending on the duration of exposure to toxin, as well as the resting period. Evident reversible processes were found after short term exposure followed by a long resting period, while regeneration processes were not pronounced after chronic poisoning followed by a short resting period., Ogled je izveden na 48 Hybro brojlera podeljenih u četiri grupe Eksperimentalne grupe su svakodnevno tretirane T-2 toksinom u količini od 0.02 mg/kg TM tokom 7, 14 ili 21 dana, dok je brojlerima kontrolne grupe aplikovana smeša etanola i fiziološkog rastvora. Uzorci jetre za patohistološka ispitivanja uzeti su nakon završetka tretmana, a preostali brojleri su hranjeni i pojeni bez aplikacije toksina do 42. dana kada su uzorci jetre ponovo uzeti. U jetri tretiranih brojlera utvrđen je histogenetski razvoj distrofičnih i discikličnih promena. Morfološke alteracije jetre razlikovale su se po karakteru, intenzitetu i raširenosti u zavisnosti od dužine ekspozicije brojlera, kao i od ukupne količine aplikovanog toksina. Stepen sanacionih procesa zavisio je od dužine ekspozicije brojlera, kao i od vremena oporavka. Reparacioni procesi su izraženi nakon kratkotrajnog delovanja T-2 toksina praćenog dugim periodom oporavka, dok se nakon hroničnog trovanja i kratkog vremena oporavka ne uočavaju regeneracioni procesi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pathomorphological alterations and degree of reparatory processes in the liver of broilers treated with t-2 toxin, Patohistološke promene i stepen sanacionih procesa u jetri brojlera tretiranih T-2 toksinom",
volume = "53",
number = "1",
pages = "27-34",
doi = "10.2298/AVB0301027S"
}

Šefer, D., Knežević, M., Sinovec, S. M.,& Sinovec, Z.. (2003). Pathomorphological alterations and degree of reparatory processes in the liver of broilers treated with t-2 toxin. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(1), 27-34.
https://doi.org/10.2298/AVB0301027S

Šefer D, Knežević M, Sinovec SM, Sinovec Z. Pathomorphological alterations and degree of reparatory processes in the liver of broilers treated with t-2 toxin. in Acta Veterinaria-Beograd. 2003;53(1):27-34.
doi:10.2298/AVB0301027S .

Šefer, Dragan, Knežević, Milijana, Sinovec, Snežana M., Sinovec, Zlatan, "Pathomorphological alterations and degree of reparatory processes in the liver of broilers treated with t-2 toxin" in Acta Veterinaria-Beograd, 53, no. 1 (2003):27-34,
https://doi.org/10.2298/AVB0301027S . .

TY  - JOUR
AU  - Jelesijević, Tomislav
AU  - Jovanović, Milijan
AU  - Knežević, Milijana
AU  - Aleksić-Kovačević, Sanja
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/264
AB  - In this retrospective study, quantitative and qualitative analyses of argyrophil nucleolar organizer regions (AgNORs) in 54 malignant and 18 benign canine mammary gland tumors were made. Statistical analysis showed a significant difference in the mean number of AgNORs per cell between benign and malignant tumors (p lt 0.01). There was no significant difference in the mean number of AgNORs per cell between complex carcinomas, simple carcinomas and carcinomas in mixed tumors (p>0.05), as well as between adenomas and benign mixed tumors (p>0.05). Six different patterns of AgNOR distribution were observed. Types I, II, III and in only two cases, type IV, were observed in benign tumors, while all six types were observed in malignant tumors. The predominant types in malignant tumors were III, IV, V and VI. Cell types II, III and IV were predominant in malignant tumors with 5.5-7 AgNORs per cell, while cell types V and VI were dominant in tumors with more than 7 AgNORs per cell.
AB  - U ovoj retrospektivnoj studiji izvršena je kvalitativna i kvantitativna analiza arigrofilnih organizatora nukleolusa (AgNORs), 54 maligna i 18 benignih tumora mlečne žlezde pasa. Uočene su statistički značajne razlike u prosečnom broju AgNORs po ćeliji između benignih i malignih tumora (p lt 0.01). Nisu dokazane statistički značajne razlike u prosečnom broju AgNORs po ćeliji između kompleksnih karcinoma, prostih karcinoma i karcinoma u mešovitom tumoru (p>0.05). Zapaženo je šest različitih tipova distribucije AgNORs. Tipovi I, II, III i samo u dva slučaja tip IV su uočeni kod benignih tumora, dok su kod malignih tumora uočeno svih šest tipova. Dominantni tipovi kod malignih tumora su III, IV, V and VI. Ćelije tipa II, III i IV dominiraju kod tumora kji imaju prosečno 5.5-7 AgNORs po ćeliji, dok tipovi V i VI su dominantni kod tumora koji imaju prosečno više od 7 AgNORs po ćeliji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Quantitative and qualitative analysis of Ag-NOR in benign and malignant canine mammary gland tumors
T1  - Kvantitativna i kvalitativna analiza Ag-NOR kod benignih i malignih tumora mlečne žlezde kuja
VL  - 53
IS  - 5-6
SP  - 353
EP  - 360
DO  - 10.2298/AVB0306353J
ER  - 

@article{
author = "Jelesijević, Tomislav and Jovanović, Milijan and Knežević, Milijana and Aleksić-Kovačević, Sanja",
year = "2003",
abstract = "In this retrospective study, quantitative and qualitative analyses of argyrophil nucleolar organizer regions (AgNORs) in 54 malignant and 18 benign canine mammary gland tumors were made. Statistical analysis showed a significant difference in the mean number of AgNORs per cell between benign and malignant tumors (p lt 0.01). There was no significant difference in the mean number of AgNORs per cell between complex carcinomas, simple carcinomas and carcinomas in mixed tumors (p>0.05), as well as between adenomas and benign mixed tumors (p>0.05). Six different patterns of AgNOR distribution were observed. Types I, II, III and in only two cases, type IV, were observed in benign tumors, while all six types were observed in malignant tumors. The predominant types in malignant tumors were III, IV, V and VI. Cell types II, III and IV were predominant in malignant tumors with 5.5-7 AgNORs per cell, while cell types V and VI were dominant in tumors with more than 7 AgNORs per cell., U ovoj retrospektivnoj studiji izvršena je kvalitativna i kvantitativna analiza arigrofilnih organizatora nukleolusa (AgNORs), 54 maligna i 18 benignih tumora mlečne žlezde pasa. Uočene su statistički značajne razlike u prosečnom broju AgNORs po ćeliji između benignih i malignih tumora (p lt 0.01). Nisu dokazane statistički značajne razlike u prosečnom broju AgNORs po ćeliji između kompleksnih karcinoma, prostih karcinoma i karcinoma u mešovitom tumoru (p>0.05). Zapaženo je šest različitih tipova distribucije AgNORs. Tipovi I, II, III i samo u dva slučaja tip IV su uočeni kod benignih tumora, dok su kod malignih tumora uočeno svih šest tipova. Dominantni tipovi kod malignih tumora su III, IV, V and VI. Ćelije tipa II, III i IV dominiraju kod tumora kji imaju prosečno 5.5-7 AgNORs po ćeliji, dok tipovi V i VI su dominantni kod tumora koji imaju prosečno više od 7 AgNORs po ćeliji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Quantitative and qualitative analysis of Ag-NOR in benign and malignant canine mammary gland tumors, Kvantitativna i kvalitativna analiza Ag-NOR kod benignih i malignih tumora mlečne žlezde kuja",
volume = "53",
number = "5-6",
pages = "353-360",
doi = "10.2298/AVB0306353J"
}

Jelesijević, T., Jovanović, M., Knežević, M.,& Aleksić-Kovačević, S.. (2003). Quantitative and qualitative analysis of Ag-NOR in benign and malignant canine mammary gland tumors. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 353-360.
https://doi.org/10.2298/AVB0306353J

Jelesijević T, Jovanović M, Knežević M, Aleksić-Kovačević S. Quantitative and qualitative analysis of Ag-NOR in benign and malignant canine mammary gland tumors. in Acta Veterinaria-Beograd. 2003;53(5-6):353-360.
doi:10.2298/AVB0306353J .

Jelesijević, Tomislav, Jovanović, Milijan, Knežević, Milijana, Aleksić-Kovačević, Sanja, "Quantitative and qualitative analysis of Ag-NOR in benign and malignant canine mammary gland tumors" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):353-360,
https://doi.org/10.2298/AVB0306353J . .

TY  - JOUR
AU  - Aleksić-Kovačević, Sanja
AU  - Knežević, Milijana
AU  - Jelesijević, Tomislav
AU  - Jovanović, Milijan
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/232
AB  - TSE represents a group of neurodegenerative diseases in animals and humans characterized by progressive tendency, long incubation period and fatal ending. Animal diseases are scrapie in sheep, transmissive mink encephalopathy (TME), chronic wasting disease of deer (CWD), bovine spongiform encephalopathy (BSE), feline spongiform encephalopathy (FSE) and spongiform encephalopathy's in some zoo animals. In humans, five diseases are known: Creucfeldt-Jakob disease (CJD), Gerstmann-Straussler-Scheinker syndrome (GSS), kuru, fatal family insomnia, and new variant of CJD (vCJD). Basic pathogenic component is specific form of PrPsc fibrilar protein. There are no commercial diagnostic tests for PrPsc detection in live animals available at the moment, so the pathogen has to be confirmed by pathohystological, immunohystochemical and electron-microscopy examinations on tissue samples of the nervous, rarely lymphatic, system. In the recent years, in the field of routine confirmatory diagnostic, immunoblotting method - Priantes takes the first place. A routine, fast diagnostic test ELISA, has also been used (Biorad and Enfer). All the three methods mentioned above have been validated as 100% specific and are accepted by the European Community for diagnostic and control of transmissive encephalopathies.
AB  - Transmisivne spongiformne encefalopatije predstavljaju grupu neurodegenerativnih oboljenja životinja i ljudi sa dugom inkubacijom progresivnim tokom i fatalnim ishodom. Ovde spadaju oboljenja životinja kao što je skrepi kod ovaca, prenosiva encefalopatija nerčeva, bolest kroničnog slabljenja jelena, spongiformna encefalopatija goveda, spongiformna encefalopatija mačaka i spongiformne encefalopatije kod nekih životinja u zoološkim vrtovima. Kod ljudi je poznato pet različitih oboljenja kao što su Creutzfeldt-Jakobova bolest, Gerstmann-Straussler-Scheinkerov sindrom, kuru fatalna familijarna insomnia, i nova varijanta CJD. Osnovna komponenta uzročnika svih navedenih bolesti je specifična forma PrPK flbrilarnog proteina. Danas još uvek nema dostupnih komercijalnih dijagnostičkih testova za detekciju PrPsc na živim životinjama, pa se agens dokazuje patohistološkim, imunohistohemijskim i elektronmikroskopskim ispitivanjima na uzorcima tkiva nervnog, a rede limfatičnog sistema. Poslednjih godina u rutinskoj i konfirmativnoj dijagnostici neprikosnovena je imunobloting metoda - Priantes. Od brzih testova u rutinskoj dijagnostici koriste se još ELISA (Biorad i Enfer). Sve tri navedene metode su vrednovane kao 100% specifične i prihvaćene su od strane Evropske zajednice za dijagnostiku i kontrolu transmisivnih encefalopatija.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Transmissive spongiform encephalopathies (TSE) diagnostic methods
T1  - Metode dijagnostike transmisivnih spongiformnih encefalopatija - TSE
VL  - 44
IS  - 3-4
SP  - 145
EP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_232
ER  - 

@article{
author = "Aleksić-Kovačević, Sanja and Knežević, Milijana and Jelesijević, Tomislav and Jovanović, Milijan",
year = "2003",
abstract = "TSE represents a group of neurodegenerative diseases in animals and humans characterized by progressive tendency, long incubation period and fatal ending. Animal diseases are scrapie in sheep, transmissive mink encephalopathy (TME), chronic wasting disease of deer (CWD), bovine spongiform encephalopathy (BSE), feline spongiform encephalopathy (FSE) and spongiform encephalopathy's in some zoo animals. In humans, five diseases are known: Creucfeldt-Jakob disease (CJD), Gerstmann-Straussler-Scheinker syndrome (GSS), kuru, fatal family insomnia, and new variant of CJD (vCJD). Basic pathogenic component is specific form of PrPsc fibrilar protein. There are no commercial diagnostic tests for PrPsc detection in live animals available at the moment, so the pathogen has to be confirmed by pathohystological, immunohystochemical and electron-microscopy examinations on tissue samples of the nervous, rarely lymphatic, system. In the recent years, in the field of routine confirmatory diagnostic, immunoblotting method - Priantes takes the first place. A routine, fast diagnostic test ELISA, has also been used (Biorad and Enfer). All the three methods mentioned above have been validated as 100% specific and are accepted by the European Community for diagnostic and control of transmissive encephalopathies., Transmisivne spongiformne encefalopatije predstavljaju grupu neurodegenerativnih oboljenja životinja i ljudi sa dugom inkubacijom progresivnim tokom i fatalnim ishodom. Ovde spadaju oboljenja životinja kao što je skrepi kod ovaca, prenosiva encefalopatija nerčeva, bolest kroničnog slabljenja jelena, spongiformna encefalopatija goveda, spongiformna encefalopatija mačaka i spongiformne encefalopatije kod nekih životinja u zoološkim vrtovima. Kod ljudi je poznato pet različitih oboljenja kao što su Creutzfeldt-Jakobova bolest, Gerstmann-Straussler-Scheinkerov sindrom, kuru fatalna familijarna insomnia, i nova varijanta CJD. Osnovna komponenta uzročnika svih navedenih bolesti je specifična forma PrPK flbrilarnog proteina. Danas još uvek nema dostupnih komercijalnih dijagnostičkih testova za detekciju PrPsc na živim životinjama, pa se agens dokazuje patohistološkim, imunohistohemijskim i elektronmikroskopskim ispitivanjima na uzorcima tkiva nervnog, a rede limfatičnog sistema. Poslednjih godina u rutinskoj i konfirmativnoj dijagnostici neprikosnovena je imunobloting metoda - Priantes. Od brzih testova u rutinskoj dijagnostici koriste se još ELISA (Biorad i Enfer). Sve tri navedene metode su vrednovane kao 100% specifične i prihvaćene su od strane Evropske zajednice za dijagnostiku i kontrolu transmisivnih encefalopatija.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Transmissive spongiform encephalopathies (TSE) diagnostic methods, Metode dijagnostike transmisivnih spongiformnih encefalopatija - TSE",
volume = "44",
number = "3-4",
pages = "145-148",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_232"
}

Aleksić-Kovačević, S., Knežević, M., Jelesijević, T.,& Jovanović, M.. (2003). Transmissive spongiform encephalopathies (TSE) diagnostic methods. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 44(3-4), 145-148.
https://hdl.handle.net/21.15107/rcub_veterinar_232

Aleksić-Kovačević S, Knežević M, Jelesijević T, Jovanović M. Transmissive spongiform encephalopathies (TSE) diagnostic methods. in Tehnologija mesa. 2003;44(3-4):145-148.
https://hdl.handle.net/21.15107/rcub_veterinar_232 .

Aleksić-Kovačević, Sanja, Knežević, Milijana, Jelesijević, Tomislav, Jovanović, Milijan, "Transmissive spongiform encephalopathies (TSE) diagnostic methods" in Tehnologija mesa, 44, no. 3-4 (2003):145-148,
https://hdl.handle.net/21.15107/rcub_veterinar_232 .