TY  - JOUR
AU  - Horyanto, Darwin
AU  - Bajagai, Yadav S.
AU  - Kayal, Advait
AU  - von Hellens, Juhani
AU  - Chen, Xiaojing
AU  - Van, Thi Thu Hao
AU  - Radovanović, Anita
AU  - Stanley, Dragana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3700
AB  - The supplementation of antimicrobial growth promoters (AGPs) has been banned in many
countries because of the emergence of antimicrobial-resistant pathogens in poultry products and
the environment. Probiotics have been broadly studied and demonstrated as a promising AGP
substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product
on broiler production performance and gut microbial profile in a dexamethasone-induced leaky
gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into
four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented
with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing
a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)).
The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota
were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved
production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on
microbiota was observed in other gut sections.
PB  - MDPI
T2  - Microorganisms
T1  - Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model
VL  - 12
IS  - 2
SP  - 419
DO  - 10.3390/microorganisms12020419
ER  - 

@article{
author = "Horyanto, Darwin and Bajagai, Yadav S. and Kayal, Advait and von Hellens, Juhani and Chen, Xiaojing and Van, Thi Thu Hao and Radovanović, Anita and Stanley, Dragana",
year = "2024",
abstract = "The supplementation of antimicrobial growth promoters (AGPs) has been banned in many
countries because of the emergence of antimicrobial-resistant pathogens in poultry products and
the environment. Probiotics have been broadly studied and demonstrated as a promising AGP
substitute. Our study is centred on the effects of a multi-strain Bacillus-based probiotic product
on broiler production performance and gut microbial profile in a dexamethasone-induced leaky
gut challenge. Two hundred and fifty-six broiler chicks were hatched and randomly assigned into
four groups (wheat-soybean meal basal diet (BD) = non-supplemented control (C), BD supplemented
with dexamethasone in week 4 (CD), BD containing a probiotic from day one (P), and BD containing
a probiotic from day one and supplemented with dexamethasone during challenge week 4 (PD)).
The production performance and caecal, gizzard, jejunal lumen and jejunal mucosa swab microbiota
were studied by 16S rRNA gene sequencing. The Bacillus probiotic product significantly improved
production performance and altered caecal gut microbiota (p ≤ 0.05), but no significant impact on
microbiota was observed in other gut sections.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model",
volume = "12",
number = "2",
pages = "419",
doi = "10.3390/microorganisms12020419"
}

Horyanto, D., Bajagai, Y. S., Kayal, A., von Hellens, J., Chen, X., Van, T. T. H., Radovanović, A.,& Stanley, D.. (2024). Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model. in Microorganisms
MDPI., 12(2), 419.
https://doi.org/10.3390/microorganisms12020419

Horyanto D, Bajagai YS, Kayal A, von Hellens J, Chen X, Van TTH, Radovanović A, Stanley D. Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model. in Microorganisms. 2024;12(2):419.
doi:10.3390/microorganisms12020419 .

Horyanto, Darwin, Bajagai, Yadav S., Kayal, Advait, von Hellens, Juhani, Chen, Xiaojing, Van, Thi Thu Hao, Radovanović, Anita, Stanley, Dragana, "Bacillus amyloliquefaciens probiotics mix supplementation in a broiler leaky gut model" in Microorganisms, 12, no. 2 (2024):419,
https://doi.org/10.3390/microorganisms12020419 . .

TY  - JOUR
AU  - Kayal, Advait
AU  - Stanley, Dragana
AU  - Radovanović, Anita
AU  - Horyanto, Darwin
AU  - Thi Thu, Thi Thu
AU  - Bajagai, Yadav S.
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2795
AB  - The concept of designer microbiota in chicken is focused on early exposure of the hatchlings
to pathogen-free microbiota inoculum, limiting the early access to harmful and pathogenic
microorganisms, thus promoting colonisation of the gut with beneficial and natural poultry microbiota.
In this study, we controlled colonisation of the intestine in broiler chickens in a large-scale
industrial setting via at-hatch administration of a commercial product containing a highly diverse
microbiota originating from the chicken caecum. The treatment significantly transformed the microbiota
membership in the crop, proventriculus, jejunum and caecum and significantly altered the
taxa abundance in the jejunum, jejunum mucosa, and caecum estimated using PERMANOVA and
unweighted and weighted UniFrac distances, respectively. The treatment also improved the growth
rate in chickens with no significant alteration in feed conversion ratio. A comparison of inoculum
product microbiota structure revealed that the inoculum had the highest Shannon diversity index
compared to all investigated gut sections, and the number of Observed Species second only to the
caecal community. PCoA plots using weighted or unweighted UniFrac placed the inoculum samples
together with the samples from the caecal origin.
PB  - MDPI
T2  - Animals
T1  - Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System
VL  - 12
SP  - 3296
DO  - 10.3390/ani12233296
ER  - 

@article{
author = "Kayal, Advait and Stanley, Dragana and Radovanović, Anita and Horyanto, Darwin and Thi Thu, Thi Thu and Bajagai, Yadav S.",
year = "2022",
abstract = "The concept of designer microbiota in chicken is focused on early exposure of the hatchlings
to pathogen-free microbiota inoculum, limiting the early access to harmful and pathogenic
microorganisms, thus promoting colonisation of the gut with beneficial and natural poultry microbiota.
In this study, we controlled colonisation of the intestine in broiler chickens in a large-scale
industrial setting via at-hatch administration of a commercial product containing a highly diverse
microbiota originating from the chicken caecum. The treatment significantly transformed the microbiota
membership in the crop, proventriculus, jejunum and caecum and significantly altered the
taxa abundance in the jejunum, jejunum mucosa, and caecum estimated using PERMANOVA and
unweighted and weighted UniFrac distances, respectively. The treatment also improved the growth
rate in chickens with no significant alteration in feed conversion ratio. A comparison of inoculum
product microbiota structure revealed that the inoculum had the highest Shannon diversity index
compared to all investigated gut sections, and the number of Observed Species second only to the
caecal community. PCoA plots using weighted or unweighted UniFrac placed the inoculum samples
together with the samples from the caecal origin.",
publisher = "MDPI",
journal = "Animals",
title = "Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System",
volume = "12",
pages = "3296",
doi = "10.3390/ani12233296"
}

Kayal, A., Stanley, D., Radovanović, A., Horyanto, D., Thi Thu, T. T.,& Bajagai, Y. S.. (2022). Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System. in Animals
MDPI., 12, 3296.
https://doi.org/10.3390/ani12233296

Kayal A, Stanley D, Radovanović A, Horyanto D, Thi Thu TT, Bajagai YS. Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System. in Animals. 2022;12:3296.
doi:10.3390/ani12233296 .

Kayal, Advait, Stanley, Dragana, Radovanović, Anita, Horyanto, Darwin, Thi Thu, Thi Thu, Bajagai, Yadav S., "Controlled Intestinal Microbiota Colonisation in Broilers under the Industrial Production System" in Animals, 12 (2022):3296,
https://doi.org/10.3390/ani12233296 . .