@conference{
author = "Ćupić Miladinović, Dejana and Peković, Sanja and Dacić, Sanja and Filipović, Isidora and Životić, Maja and Đorđević, Milena and Blagojević, Miloš and Nešić, Ivana and Borozan, Sunčica",
year = "2019",
abstract = "The aim of this study was to investigate the influence of vitamin B1 (thiamine) following thepathohystologycalchanges in the hippocampus, cerebellum and cerebral cortex of Japanese quail (Coturnix japanica) treated with chlorpyrifos.In this study we also assessed the antioxidative activity of thiamine in the brain by monitoring the nitrite concentration(NO2-), parameter of oxidative/nitrosative stress and activities of agents of cellular detoxification such as glutathione(GSH) and glutathione S-transferase(GST).
The study was conducted onforty male Japanese quails (2 controls and 2 experimental groups, n= 10), 3-4 weeks old. One controlgroup was treated only with vitamin B1, while the second one receivedpurecorn oil.CPFdissolved in corn oil was administeredto quails by gavage for 7 consecutive days at dose of 3 mg/kg BW while another groups wastreated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration for 7 consecutive days.
Our studies have shown that CPF has ledto increase in the concentration of NO2-,but after thiamine treatment there has been a decrease. Also CPF has led to small changes in GSH and GST levels, while groups treated with vitamin B1showedsignificantly (p< 0.0001)increased activity of these parameteres, proving very important role of thiamine in the detoxification and elimination of pesticides.
In hippocampus groups that received CPF showed signs of edema with numerous damaged neurons, especially in pyramidal layer, while in groups that received vitamin B1 along with CPF, pathological changes were similar, but less prominent.In cerebellum groups that received CPF showed large number of degenerated Purkinje cells, while with vitamin B1 the reduction of degenerated neurons is present.Cerebral cortex showed degeneration with pycnotic nuclei of many neurons, edema and congestion in groups which received CPF and also similar changes were found after application of B1.
Overall these results confirm that CPF causes oxidative stress and degenerative changes, but also support the hypothesis that thiamine belongs to the group of "antistress vitamins".",
publisher = "Beograd: Društvo za neuronauke Srbije",
journal = "FENS - Regional meeting, Belgrade, July 10-13 2019",
title = "What happens after application of thiamine in the brain of Japanese quailstreated with chlorpyrifos",
pages = "486-486",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2640"
}
