TY  - JOUR
AU  - van Hage, M.
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/986
PB  - Wiley, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Diversity of allergens contained in dog saliva REPLY
VL  - 68
IS  - 11
SP  - 1485
EP  - 1486
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_986
ER  - 

@article{
author = "van Hage, M. and Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H.",
year = "2013",
publisher = "Wiley, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Diversity of allergens contained in dog saliva REPLY",
volume = "68",
number = "11",
pages = "1485-1486",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_986"
}

van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Gronlund, H.. (2013). Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley, Hoboken., 68(11), 1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986

van Hage M, Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H. Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(11):1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986 .

van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., "Diversity of allergens contained in dog saliva REPLY" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 11 (2013):1485-1486,
https://hdl.handle.net/21.15107/rcub_veterinar_986 .

TY  - JOUR
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1026
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - an important source of dog allergens
VL  - 68
IS  - 5
SP  - 585
EP  - 592
DO  - 10.1111/all.12130
ER  - 

@article{
author = "Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H. and van Hage, M.",
year = "2013",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
number = "5",
pages = "585-592",
doi = "10.1111/all.12130"
}

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 68(5), 585-592.
https://doi.org/10.1111/all.12130

Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(5):585-592.
doi:10.1111/all.12130 .

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., van Hage, M., "Dog saliva - an important source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 5 (2013):585-592,
https://doi.org/10.1111/all.12130 . .