TY  - JOUR
AU  - Kim, W.K.
AU  - Karabasil, Nedjeljko
AU  - Bulajić, Snežana
AU  - Dunkley, K.D.
AU  - Callaway, T.R.
AU  - Poole, T.L.
AU  - Ricke, S.C.
AU  - Anderson, Robin C.
AU  - Nisbet, David J.
PY  - 2005
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/342
AB  - The objective of the study was to determine the frequency of spontaneous acquisition of resistance to select antibiotics by Salmonella Typhimurium (ST) when grown in glucose amended continuous flow culture at slow (D = 0.025 h -1) or fast (D = 0.27 h-1) dilution rates. The bacterium was grown in LB minimal medium (pH 6.25) containing no antibiotics. Upon achieving steady state, samples were plated to tryptic soy agar (TSA) alone or supplemented (per ml) with 2 and 16 μg oxytetracycline, 4 and 16 μg tetracycline, 2 and 64 μg kanamycin, and 0.25 and 2 μg enrofloxacin. Regardless of growth rate, CFU of resistant ST from the TSA containing antibiotics was less than 2 × 101 except for 2 μg kanamycin and 0.25 μg enrofloxacin treatments (higher than 1 × 109 and 4 × 107 CFU of resistant ST for trials 1 and 2, respectively). Frequency of recovering resistant ST from the TSA containing the higher antibiotic concentrations was less than 1 in 109 for all antibiotics, but was higher on the media containing 2 μg kanamycin and 0.25 μg enrofloxacin at both slow and fast growth rates. In general, minimal susceptibility differences were detected for isolates from slow and fast dilution rates.
PB  - Taylor and Francis Inc.
T2  - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
T1  - Comparison of spontaneous antibiotic resistance frequency of Salmonella typhimurium growth in glucose amended continuous culture at slow and fast dilution rates
VL  - 40
IS  - 3
SP  - 475
EP  - 484
DO  - 10.1081/PFC-200047598
ER  - 

@article{
author = "Kim, W.K. and Karabasil, Nedjeljko and Bulajić, Snežana and Dunkley, K.D. and Callaway, T.R. and Poole, T.L. and Ricke, S.C. and Anderson, Robin C. and Nisbet, David J.",
year = "2005",
abstract = "The objective of the study was to determine the frequency of spontaneous acquisition of resistance to select antibiotics by Salmonella Typhimurium (ST) when grown in glucose amended continuous flow culture at slow (D = 0.025 h -1) or fast (D = 0.27 h-1) dilution rates. The bacterium was grown in LB minimal medium (pH 6.25) containing no antibiotics. Upon achieving steady state, samples were plated to tryptic soy agar (TSA) alone or supplemented (per ml) with 2 and 16 μg oxytetracycline, 4 and 16 μg tetracycline, 2 and 64 μg kanamycin, and 0.25 and 2 μg enrofloxacin. Regardless of growth rate, CFU of resistant ST from the TSA containing antibiotics was less than 2 × 101 except for 2 μg kanamycin and 0.25 μg enrofloxacin treatments (higher than 1 × 109 and 4 × 107 CFU of resistant ST for trials 1 and 2, respectively). Frequency of recovering resistant ST from the TSA containing the higher antibiotic concentrations was less than 1 in 109 for all antibiotics, but was higher on the media containing 2 μg kanamycin and 0.25 μg enrofloxacin at both slow and fast growth rates. In general, minimal susceptibility differences were detected for isolates from slow and fast dilution rates.",
publisher = "Taylor and Francis Inc.",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural",
title = "Comparison of spontaneous antibiotic resistance frequency of Salmonella typhimurium growth in glucose amended continuous culture at slow and fast dilution rates",
volume = "40",
number = "3",
pages = "475-484",
doi = "10.1081/PFC-200047598"
}

Kim, W.K., Karabasil, N., Bulajić, S., Dunkley, K.D., Callaway, T.R., Poole, T.L., Ricke, S.C., Anderson, R. C.,& Nisbet, D. J.. (2005). Comparison of spontaneous antibiotic resistance frequency of Salmonella typhimurium growth in glucose amended continuous culture at slow and fast dilution rates. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
Taylor and Francis Inc.., 40(3), 475-484.
https://doi.org/10.1081/PFC-200047598

Kim W, Karabasil N, Bulajić S, Dunkley K, Callaway T, Poole T, Ricke S, Anderson RC, Nisbet DJ. Comparison of spontaneous antibiotic resistance frequency of Salmonella typhimurium growth in glucose amended continuous culture at slow and fast dilution rates. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural. 2005;40(3):475-484.
doi:10.1081/PFC-200047598 .

Kim, W.K., Karabasil, Nedjeljko, Bulajić, Snežana, Dunkley, K.D., Callaway, T.R., Poole, T.L., Ricke, S.C., Anderson, Robin C., Nisbet, David J., "Comparison of spontaneous antibiotic resistance frequency of Salmonella typhimurium growth in glucose amended continuous culture at slow and fast dilution rates" in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 40, no. 3 (2005):475-484,
https://doi.org/10.1081/PFC-200047598 . .