TY  - JOUR
AU  - Yu, Sung Joon
AU  - Morris, Andrew
AU  - Kayal, Advait
AU  - Milošević, Ivan
AU  - Hao Van, Thi Thu
AU  - Bajagai, Yadav Sharma
AU  - Stanley, Dragana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3596
AB  - This research investigates the effects of phytogenic feed additives (PFAs) on the growth performance, gut microbial community,
and microbial metabolic functions in weaned piglets via a combined 16S rRNA gene amplicon and shotgun metagenomics
approach. A controlled trial was conducted using 200 pigs to highlight the significant influence of PFAs on gut microbiota
dynamics. Notably, the treatment group revealed an increased gut microbiota diversity, as measured with the Shannon and
Simpson indices. The increase in diversity is accompanied by an increase in beneficial bacterial taxa, such as Roseburia,
Faecalibacterium, and Prevotella, and a decline in potential pathogens like Clostridium sensu stricto 1 and Campylobacter.
Shotgun sequencing at the species level confirmed these findings. This modification in microbial profile was coupled with
an altered profile of microbial metabolic pathways, suggesting a reconfiguration of microbial function under PFA influence.
Significant shifts in overall microbial community structure by week 8 demonstrate PFA treatment’s temporal impact.
Histomorphological examination unveiled improved gut structure in PFA-treated piglets. The results of this study indicate
that the use of PFAs as dietary supplements can be an effective strategy, augmenting gut microbiota diversity, reshaping
microbial function, enhancing gut structure, and optimising intestinal health of weaned piglets providing valuable implications
for swine production.
Key points
• PFAs significantly diversify the gut microbiota in weaned piglets, aiding balance.
• Changes in gut structure due to PFAs indicate improved resistance to weaning stress.
• PFAs show potential to ease weaning stress, offering a substitute for antibiotics in piglet diets.
PB  - Springer
T2  - Applied Microbiology and Biotechnology
T1  - Pioneering gut health improvements in piglets with phytogenic feed additives
VL  - 108
SP  - 142
DO  - 10.1007/s00253-023-12925-2
ER  - 

@article{
author = "Yu, Sung Joon and Morris, Andrew and Kayal, Advait and Milošević, Ivan and Hao Van, Thi Thu and Bajagai, Yadav Sharma and Stanley, Dragana",
year = "2024",
abstract = "This research investigates the effects of phytogenic feed additives (PFAs) on the growth performance, gut microbial community,
and microbial metabolic functions in weaned piglets via a combined 16S rRNA gene amplicon and shotgun metagenomics
approach. A controlled trial was conducted using 200 pigs to highlight the significant influence of PFAs on gut microbiota
dynamics. Notably, the treatment group revealed an increased gut microbiota diversity, as measured with the Shannon and
Simpson indices. The increase in diversity is accompanied by an increase in beneficial bacterial taxa, such as Roseburia,
Faecalibacterium, and Prevotella, and a decline in potential pathogens like Clostridium sensu stricto 1 and Campylobacter.
Shotgun sequencing at the species level confirmed these findings. This modification in microbial profile was coupled with
an altered profile of microbial metabolic pathways, suggesting a reconfiguration of microbial function under PFA influence.
Significant shifts in overall microbial community structure by week 8 demonstrate PFA treatment’s temporal impact.
Histomorphological examination unveiled improved gut structure in PFA-treated piglets. The results of this study indicate
that the use of PFAs as dietary supplements can be an effective strategy, augmenting gut microbiota diversity, reshaping
microbial function, enhancing gut structure, and optimising intestinal health of weaned piglets providing valuable implications
for swine production.
Key points
• PFAs significantly diversify the gut microbiota in weaned piglets, aiding balance.
• Changes in gut structure due to PFAs indicate improved resistance to weaning stress.
• PFAs show potential to ease weaning stress, offering a substitute for antibiotics in piglet diets.",
publisher = "Springer",
journal = "Applied Microbiology and Biotechnology",
title = "Pioneering gut health improvements in piglets with phytogenic feed additives",
volume = "108",
pages = "142",
doi = "10.1007/s00253-023-12925-2"
}

Yu, S. J., Morris, A., Kayal, A., Milošević, I., Hao Van, T. T., Bajagai, Y. S.,& Stanley, D.. (2024). Pioneering gut health improvements in piglets with phytogenic feed additives. in Applied Microbiology and Biotechnology
Springer., 108, 142.
https://doi.org/10.1007/s00253-023-12925-2

Yu SJ, Morris A, Kayal A, Milošević I, Hao Van TT, Bajagai YS, Stanley D. Pioneering gut health improvements in piglets with phytogenic feed additives. in Applied Microbiology and Biotechnology. 2024;108:142.
doi:10.1007/s00253-023-12925-2 .

Yu, Sung Joon, Morris, Andrew, Kayal, Advait, Milošević, Ivan, Hao Van, Thi Thu, Bajagai, Yadav Sharma, Stanley, Dragana, "Pioneering gut health improvements in piglets with phytogenic feed additives" in Applied Microbiology and Biotechnology, 108 (2024):142,
https://doi.org/10.1007/s00253-023-12925-2 . .

TY  - JOUR
AU  - Bauer, Benjamin W.
AU  - Radovanović, Anita
AU  - Willson, Nicky-Lee
AU  - Bajagai, Yadav Sharma
AU  - Thi, Thu Hao Van
AU  - Moore, Robert J.
AU  - Stanley, Dragana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1763
AB  - Prophylactic use of antibiotics in poultry diets has been identified as a problematic practice because of its potential to exacerbate the spread of antibiotic resistance to human pathogens. A range of countries have opted to completely ban the use of antibiotics in animal feed. The animal production industries are looking for alternative ways to effectively control pathogens while providing the performance benefits previously secured by antibiotics in feed. Here, we present evidence that oregano (Origanum vulgare) could be a potential alternative for pathogen control in the poultry industry. Broiler diets were supplemented with oregano powder (0%, 0.5%, 1%, and 2%) for six weeks. The capacity for pathogen control was estimated by microbiota profiling of the jejunum, ileum, and caecum content, and in the faeces, by 16S rRNA gene amplicon sequencing. The concentrations of short-chain fatty acids in the caecal content were also measured, as were villus/crypt parameters in the ileum. There were no differences among treatments in weight gain, feed intake, or the concentration of short-chain fatty acids. The height, width, and the surface area of villi in the ileum were not influenced by oregano addition. However, 1% and 2% of oregano produced a significant increase in the villus height to crypt depth ratio. There were no visible histopathological changes in the liver in control and treated groups. Although oregano had no significant effect on overall microbial diversity and gross composition, some specific genera, like Proteus, Klebsiella and Staphylococcus, which include known pathogens, were reduced in relative abundance by oregano treatment. Bifidobacterium, recognized as a beneficial and probiotic genus, was also suppressed by the oregano treatment.
PB  - Elsevier Sci Ltd, Oxford
T2  - Heliyon
T1  - Oregano: A potential prophylactic treatment for the intestinal microbiota
VL  - 5
IS  - 10
SP  - e02625
DO  - 10.1016/j.heliyon.2019.e02625
ER  - 

@article{
author = "Bauer, Benjamin W. and Radovanović, Anita and Willson, Nicky-Lee and Bajagai, Yadav Sharma and Thi, Thu Hao Van and Moore, Robert J. and Stanley, Dragana",
year = "2019",
abstract = "Prophylactic use of antibiotics in poultry diets has been identified as a problematic practice because of its potential to exacerbate the spread of antibiotic resistance to human pathogens. A range of countries have opted to completely ban the use of antibiotics in animal feed. The animal production industries are looking for alternative ways to effectively control pathogens while providing the performance benefits previously secured by antibiotics in feed. Here, we present evidence that oregano (Origanum vulgare) could be a potential alternative for pathogen control in the poultry industry. Broiler diets were supplemented with oregano powder (0%, 0.5%, 1%, and 2%) for six weeks. The capacity for pathogen control was estimated by microbiota profiling of the jejunum, ileum, and caecum content, and in the faeces, by 16S rRNA gene amplicon sequencing. The concentrations of short-chain fatty acids in the caecal content were also measured, as were villus/crypt parameters in the ileum. There were no differences among treatments in weight gain, feed intake, or the concentration of short-chain fatty acids. The height, width, and the surface area of villi in the ileum were not influenced by oregano addition. However, 1% and 2% of oregano produced a significant increase in the villus height to crypt depth ratio. There were no visible histopathological changes in the liver in control and treated groups. Although oregano had no significant effect on overall microbial diversity and gross composition, some specific genera, like Proteus, Klebsiella and Staphylococcus, which include known pathogens, were reduced in relative abundance by oregano treatment. Bifidobacterium, recognized as a beneficial and probiotic genus, was also suppressed by the oregano treatment.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Heliyon",
title = "Oregano: A potential prophylactic treatment for the intestinal microbiota",
volume = "5",
number = "10",
pages = "e02625",
doi = "10.1016/j.heliyon.2019.e02625"
}

Bauer, B. W., Radovanović, A., Willson, N., Bajagai, Y. S., Thi, T. H. V., Moore, R. J.,& Stanley, D.. (2019). Oregano: A potential prophylactic treatment for the intestinal microbiota. in Heliyon
Elsevier Sci Ltd, Oxford., 5(10), e02625.
https://doi.org/10.1016/j.heliyon.2019.e02625

Bauer BW, Radovanović A, Willson N, Bajagai YS, Thi THV, Moore RJ, Stanley D. Oregano: A potential prophylactic treatment for the intestinal microbiota. in Heliyon. 2019;5(10):e02625.
doi:10.1016/j.heliyon.2019.e02625 .

Bauer, Benjamin W., Radovanović, Anita, Willson, Nicky-Lee, Bajagai, Yadav Sharma, Thi, Thu Hao Van, Moore, Robert J., Stanley, Dragana, "Oregano: A potential prophylactic treatment for the intestinal microbiota" in Heliyon, 5, no. 10 (2019):e02625,
https://doi.org/10.1016/j.heliyon.2019.e02625 . .