TY  - CONF
AU  - Krstić, Milena
AU  - Santibanez, Juan Francisco
AU  - Poljarević, Jelena
AU  - Nikolić, Stefan
AU  - Grguric-Šipka, Sanja R.
AU  - Borozan, Sunčica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2797
AB  - U cilju pronalaženja adekvatne terapije u lecenju maligniteta kompleksi rutenijuma
pokazali su zavidan potencijal. Kompleksi Ru(II) sa N-alkilfenotiazinima,
hlorpromazinom, trifluoperazinom i tioridazinom, korišceni su u ispitivanju mogucih
puteva apoptoze u K562 celijama. Ispitivana je spektrofotometrijski ekstracelularna LDH,
ekspresija COX-2, t-JNK, p-JNK i b-aktina imunohemijski nakon SDS elektroforeze.
Kompleks Ru(II) sa trifluoperazinom u koncentraciji od 10 μM smanjuje ekspresiju t-JNK,
inhibira COX-2 oko 42%, znacajno povecava kolicinu ekstracelularne LDH u odnosu na
netretirane K562 celije i time potvrdjuje apoptozu ovih celija
AB  - Ruthenium complexes are of significant interest in the treatment of malignancies. Ru(II)
complexes with N-alkylphenothiazines (chlorpromazine, trifluoperazine, and thioridazine)
were used in the study of possible apoptosis pathways in K562 cells.
Spectrophotometrically extracellular LDH was quantified and immunochemical expression
of COX-2, t-JNK, p-JNK and b-actin after SDS electrophoresis was determined. The
Ru(II) complex with trifluoperazine at a concentration of 10 μM reduced t-JNK
expression, inhibited COX-2 by about 42%, significantly increased the amount of
extracellular LDH compared to the untreated K562 cells and thus confirmed apoptosis.
PB  - Beograd : Razvojno-istraživački centar grafičkog inženjerstva TMF
C3  - 58. savetovanje Srpskog hemijskog društva, Beograd, 9 - 10. jun 2022.
T1  - Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije
T1  - Influence of Ru(II) complex on possible pathways of apoptosis in
K562 leukemia cells
SP  - 89
EP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2797
ER  - 

@conference{
author = "Krstić, Milena and Santibanez, Juan Francisco and Poljarević, Jelena and Nikolić, Stefan and Grguric-Šipka, Sanja R. and Borozan, Sunčica",
year = "2022",
abstract = "U cilju pronalaženja adekvatne terapije u lecenju maligniteta kompleksi rutenijuma
pokazali su zavidan potencijal. Kompleksi Ru(II) sa N-alkilfenotiazinima,
hlorpromazinom, trifluoperazinom i tioridazinom, korišceni su u ispitivanju mogucih
puteva apoptoze u K562 celijama. Ispitivana je spektrofotometrijski ekstracelularna LDH,
ekspresija COX-2, t-JNK, p-JNK i b-aktina imunohemijski nakon SDS elektroforeze.
Kompleks Ru(II) sa trifluoperazinom u koncentraciji od 10 μM smanjuje ekspresiju t-JNK,
inhibira COX-2 oko 42%, znacajno povecava kolicinu ekstracelularne LDH u odnosu na
netretirane K562 celije i time potvrdjuje apoptozu ovih celija, Ruthenium complexes are of significant interest in the treatment of malignancies. Ru(II)
complexes with N-alkylphenothiazines (chlorpromazine, trifluoperazine, and thioridazine)
were used in the study of possible apoptosis pathways in K562 cells.
Spectrophotometrically extracellular LDH was quantified and immunochemical expression
of COX-2, t-JNK, p-JNK and b-actin after SDS electrophoresis was determined. The
Ru(II) complex with trifluoperazine at a concentration of 10 μM reduced t-JNK
expression, inhibited COX-2 by about 42%, significantly increased the amount of
extracellular LDH compared to the untreated K562 cells and thus confirmed apoptosis.",
publisher = "Beograd : Razvojno-istraživački centar grafičkog inženjerstva TMF",
journal = "58. savetovanje Srpskog hemijskog društva, Beograd, 9 - 10. jun 2022.",
title = "Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije, Influence of Ru(II) complex on possible pathways of apoptosis in
K562 leukemia cells",
pages = "89-89",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2797"
}

Krstić, M., Santibanez, J. F., Poljarević, J., Nikolić, S., Grguric-Šipka, S. R.,& Borozan, S.. (2022). Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije. in 58. savetovanje Srpskog hemijskog društva, Beograd, 9 - 10. jun 2022.
Beograd : Razvojno-istraživački centar grafičkog inženjerstva TMF., 89-89.
https://hdl.handle.net/21.15107/rcub_veterinar_2797

Krstić M, Santibanez JF, Poljarević J, Nikolić S, Grguric-Šipka SR, Borozan S. Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije. in 58. savetovanje Srpskog hemijskog društva, Beograd, 9 - 10. jun 2022.. 2022;:89-89.
https://hdl.handle.net/21.15107/rcub_veterinar_2797 .

Krstić, Milena, Santibanez, Juan Francisco, Poljarević, Jelena, Nikolić, Stefan, Grguric-Šipka, Sanja R., Borozan, Sunčica, "Uticaj kompleksa Ru(II) na moguce puteve apoptoze u K562 celijama leukemije" in 58. savetovanje Srpskog hemijskog društva, Beograd, 9 - 10. jun 2022. (2022):89-89,
https://hdl.handle.net/21.15107/rcub_veterinar_2797 .

TY  - CONF
AU  - Petrović, Tamara
AU  - Gligorijević, Nevenka
AU  - Belaj, Ferdinand
AU  - Grgurić-Šipka, Sanja
AU  - Nikolić, Stefan
AU  - Krstić, Milena
AU  - Poljarević, Jelena
AU  - Mihajlović-Lalić, Ljiljana E.
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2798
AB  - Rhenium complexes merit particular attention in the area of metallodrug design due to rhenium’s broad spectrum of oxidation states and consequently, the possibility to design compounds of a great structural diversity. Thus, the synthesis, chemical characterization and antitumor activity in vitro of the three Re(V) complexes is described. Novel compounds were obtained via reaction of [ReOCl3(PPh3)2] with corresponding ligands (pyridine-2-carboxylic acid, 3-methylpyridine-2-carboxylic acid and 6-methylpyridine- 2-carboxylic acid) in acetonitrile at 78 °C for 3h. The complexes were fully characterized using NMR, IR, MS and elemental analysis. Their octahedral geometry with bidentate N^O ligand was confirmed by X-ray diffraction analysis. Antiproliferative effect was determined by MTT assay and only the complex with pyridine-2-carboxylic acid (1) showed dose-dependent cytotoxic potential, particularly toward triple-negative breast adenocarcinoma cells MDA-MB-231 with IC50 68.90 ±
1.73 μM and pancreatic adenocarcinoma cells PANC-1 with IC50 69.8 ± 2.3 μM. Drug combination studies in PANC-1 cells with 1 and Verapamil hydrochloride (VRP) showed slight arrest of cell cycle in the S phase and also it increase its antiproliferative potential to IC50 51.4 ± 2.8 μM. Part of the research included a depletion of the glutathione (GSH) level by L-buthionine-sulfoximine (L-BSO) at sub-toxic concentrations (100 μM) in PANC-1 cells which caused an increase of activity of 1 to the IC50 57.67 ± 6.51 μM.
C3  - Osterreichische Chemietage, Wienna, Austria, September 20-22, 2022
T1  - Oxorhenium(V) complexes in the drug combination study
SP  - 90
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2798
ER  - 

@conference{
author = "Petrović, Tamara and Gligorijević, Nevenka and Belaj, Ferdinand and Grgurić-Šipka, Sanja and Nikolić, Stefan and Krstić, Milena and Poljarević, Jelena and Mihajlović-Lalić, Ljiljana E.",
year = "2022",
abstract = "Rhenium complexes merit particular attention in the area of metallodrug design due to rhenium’s broad spectrum of oxidation states and consequently, the possibility to design compounds of a great structural diversity. Thus, the synthesis, chemical characterization and antitumor activity in vitro of the three Re(V) complexes is described. Novel compounds were obtained via reaction of [ReOCl3(PPh3)2] with corresponding ligands (pyridine-2-carboxylic acid, 3-methylpyridine-2-carboxylic acid and 6-methylpyridine- 2-carboxylic acid) in acetonitrile at 78 °C for 3h. The complexes were fully characterized using NMR, IR, MS and elemental analysis. Their octahedral geometry with bidentate N^O ligand was confirmed by X-ray diffraction analysis. Antiproliferative effect was determined by MTT assay and only the complex with pyridine-2-carboxylic acid (1) showed dose-dependent cytotoxic potential, particularly toward triple-negative breast adenocarcinoma cells MDA-MB-231 with IC50 68.90 ±
1.73 μM and pancreatic adenocarcinoma cells PANC-1 with IC50 69.8 ± 2.3 μM. Drug combination studies in PANC-1 cells with 1 and Verapamil hydrochloride (VRP) showed slight arrest of cell cycle in the S phase and also it increase its antiproliferative potential to IC50 51.4 ± 2.8 μM. Part of the research included a depletion of the glutathione (GSH) level by L-buthionine-sulfoximine (L-BSO) at sub-toxic concentrations (100 μM) in PANC-1 cells which caused an increase of activity of 1 to the IC50 57.67 ± 6.51 μM.",
journal = "Osterreichische Chemietage, Wienna, Austria, September 20-22, 2022",
title = "Oxorhenium(V) complexes in the drug combination study",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2798"
}

Petrović, T., Gligorijević, N., Belaj, F., Grgurić-Šipka, S., Nikolić, S., Krstić, M., Poljarević, J.,& Mihajlović-Lalić, L. E.. (2022). Oxorhenium(V) complexes in the drug combination study. in Osterreichische Chemietage, Wienna, Austria, September 20-22, 2022, 90-90.
https://hdl.handle.net/21.15107/rcub_veterinar_2798

Petrović T, Gligorijević N, Belaj F, Grgurić-Šipka S, Nikolić S, Krstić M, Poljarević J, Mihajlović-Lalić LE. Oxorhenium(V) complexes in the drug combination study. in Osterreichische Chemietage, Wienna, Austria, September 20-22, 2022. 2022;:90-90.
https://hdl.handle.net/21.15107/rcub_veterinar_2798 .

Petrović, Tamara, Gligorijević, Nevenka, Belaj, Ferdinand, Grgurić-Šipka, Sanja, Nikolić, Stefan, Krstić, Milena, Poljarević, Jelena, Mihajlović-Lalić, Ljiljana E., "Oxorhenium(V) complexes in the drug combination study" in Osterreichische Chemietage, Wienna, Austria, September 20-22, 2022 (2022):90-90,
https://hdl.handle.net/21.15107/rcub_veterinar_2798 .

TY  - CONF
AU  - Poljarević, Jelena
AU  - Tadić, Ana
AU  - Krstić, Milena
AU  - Mihajlović-Lalić, Ljiljana
AU  - Savić, Aleksandar
AU  - Nikolić, Stefan
AU  - Kajzergerber, Marijana
AU  - Ranđelović, Sandra
AU  - Grgurić-Šipka, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3798
AB  - U ovom radu sintetisano je četiri nova arenska kompleksa Ru(II) sa dva nesteroidna
antiimflamatorna leka, indometacin i mefenaminskom kiselininom. Kompleksi su sintetisani
polazeći od metanolnog rastvora polaznog Ru(II) polusendvič jedinjenja i baznog metanolnog rastvora odgovarajućeg liganda. Strukture novih kompleksa određene su NMR i IC
spektroskopijom, kao i masenom spektrometrijom. Pokazano je da se ligandi koordinuju
monodentatno preko karboksilnih kiseonika. Citotoksična aktivnost liganada i kompleksa
ispitana je na tri ćelijske linije humanog kancera i zdravih humanih fibroblastnih ćelija, korišćenjem MTT metode. IC50 vrednosti dobijene na ćelijama leukemije K562 bile su uporedive sa onim za cisplatinu (10.3 µM (cisplatina), 11.9 µM (kompleks 1) i 13.2 µM (kompleks 3)). Korišćenjem protočne citometrije pokazano je da ovi kompleksi pri IC50 koncentracijama zaustavljaju ćelijski ciklus u S fazi. Merenje produkcije reaktivnih kiseoničnih
DCFH-DA metodom potvrdilo je njihov potencijal da povećavaju ROS više od cisplatine.
AB  - Two non-steroidal antiinflammatory drugs, indomethacin and mefenamic acid, were
coordinated to Ru(II)-arenes to give four new complexes. These four complexes were
synthesized by mixing methanolic solution of starting half-sandwich Ru complexes with base
solution of ligand in methanol. Structures of complexes were determinated by NMR, IR
spectroscopy and mass spectrometry. It was shown that they coordinates monodentatly via
carboxylic oxygen. The cytotoxic activity of the ligands and ruthenium complexes was tested
in three human cancer cell lines and non-tumour human fetal lung fibroblast cells by MTT
assay. The IC50 values obtained in leukemia K562 cells, were comparable to cisplatin (10.3
µM (CDDP), 11.9 µM (1) and 13.2 µM (3)). Flow cytometric analysis of these conplexes,
showed that at IC50 concentrations, they arrested cell cycle progression in the S phase.
Measurement of reactive oxygen species (ROS) production, by DCFH-DA staining, confirmed
their potential to increase ROS even more than cisplatin.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 55. Savetovanje Srpskog hemijskog društva, Novi Sad, 8 - 9. jun 2018
T1  - Novi rutenijum–arenski kompleksi sa antiinflamatornim lekovima
T1  - Novel ruthenium–arene complexes with antiinflamatory drugs
SP  - 44
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3798
ER  - 

@conference{
author = "Poljarević, Jelena and Tadić, Ana and Krstić, Milena and Mihajlović-Lalić, Ljiljana and Savić, Aleksandar and Nikolić, Stefan and Kajzergerber, Marijana and Ranđelović, Sandra and Grgurić-Šipka, Sanja",
year = "2018",
abstract = "U ovom radu sintetisano je četiri nova arenska kompleksa Ru(II) sa dva nesteroidna
antiimflamatorna leka, indometacin i mefenaminskom kiselininom. Kompleksi su sintetisani
polazeći od metanolnog rastvora polaznog Ru(II) polusendvič jedinjenja i baznog metanolnog rastvora odgovarajućeg liganda. Strukture novih kompleksa određene su NMR i IC
spektroskopijom, kao i masenom spektrometrijom. Pokazano je da se ligandi koordinuju
monodentatno preko karboksilnih kiseonika. Citotoksična aktivnost liganada i kompleksa
ispitana je na tri ćelijske linije humanog kancera i zdravih humanih fibroblastnih ćelija, korišćenjem MTT metode. IC50 vrednosti dobijene na ćelijama leukemije K562 bile su uporedive sa onim za cisplatinu (10.3 µM (cisplatina), 11.9 µM (kompleks 1) i 13.2 µM (kompleks 3)). Korišćenjem protočne citometrije pokazano je da ovi kompleksi pri IC50 koncentracijama zaustavljaju ćelijski ciklus u S fazi. Merenje produkcije reaktivnih kiseoničnih
DCFH-DA metodom potvrdilo je njihov potencijal da povećavaju ROS više od cisplatine., Two non-steroidal antiinflammatory drugs, indomethacin and mefenamic acid, were
coordinated to Ru(II)-arenes to give four new complexes. These four complexes were
synthesized by mixing methanolic solution of starting half-sandwich Ru complexes with base
solution of ligand in methanol. Structures of complexes were determinated by NMR, IR
spectroscopy and mass spectrometry. It was shown that they coordinates monodentatly via
carboxylic oxygen. The cytotoxic activity of the ligands and ruthenium complexes was tested
in three human cancer cell lines and non-tumour human fetal lung fibroblast cells by MTT
assay. The IC50 values obtained in leukemia K562 cells, were comparable to cisplatin (10.3
µM (CDDP), 11.9 µM (1) and 13.2 µM (3)). Flow cytometric analysis of these conplexes,
showed that at IC50 concentrations, they arrested cell cycle progression in the S phase.
Measurement of reactive oxygen species (ROS) production, by DCFH-DA staining, confirmed
their potential to increase ROS even more than cisplatin.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "55. Savetovanje Srpskog hemijskog društva, Novi Sad, 8 - 9. jun 2018",
title = "Novi rutenijum–arenski kompleksi sa antiinflamatornim lekovima, Novel ruthenium–arene complexes with antiinflamatory drugs",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3798"
}

Poljarević, J., Tadić, A., Krstić, M., Mihajlović-Lalić, L., Savić, A., Nikolić, S., Kajzergerber, M., Ranđelović, S.,& Grgurić-Šipka, S.. (2018). Novi rutenijum–arenski kompleksi sa antiinflamatornim lekovima. in 55. Savetovanje Srpskog hemijskog društva, Novi Sad, 8 - 9. jun 2018
Beograd : Srpsko hemijsko društvo., 44-44.
https://hdl.handle.net/21.15107/rcub_veterinar_3798

Poljarević J, Tadić A, Krstić M, Mihajlović-Lalić L, Savić A, Nikolić S, Kajzergerber M, Ranđelović S, Grgurić-Šipka S. Novi rutenijum–arenski kompleksi sa antiinflamatornim lekovima. in 55. Savetovanje Srpskog hemijskog društva, Novi Sad, 8 - 9. jun 2018. 2018;:44-44.
https://hdl.handle.net/21.15107/rcub_veterinar_3798 .

Poljarević, Jelena, Tadić, Ana, Krstić, Milena, Mihajlović-Lalić, Ljiljana, Savić, Aleksandar, Nikolić, Stefan, Kajzergerber, Marijana, Ranđelović, Sandra, Grgurić-Šipka, Sanja, "Novi rutenijum–arenski kompleksi sa antiinflamatornim lekovima" in 55. Savetovanje Srpskog hemijskog društva, Novi Sad, 8 - 9. jun 2018 (2018):44-44,
https://hdl.handle.net/21.15107/rcub_veterinar_3798 .