@conference{
author = "Delić-Vujanović, Biljana and Tanasić, Ljiljana and Savić Radovanović, Radoslava",
year = "2021",
abstract = "The genus Pseudomonas is a heterogeneous and ecologically important group of
microorganisms, widely distributed in milk, water and the environment. They are
classified as opportunistic pathogenic microorganisms for humans, animals and
plants. The aim of this study was to examine the proteolytic, lipolytic activity and
biofilm formation in isolates derived from milk. The material was pooled samples of
raw milk from which isolates of Pseudomonas species (n=60) were isolated.
Proteolytic ability was tested on nutrient agar with casein and lipolytic ability on
Tributyrin agar. The ability of Pseudomonas species isolates to produce biofilm was
tested in vitro by the Crystal Violet (CV) method on microtiter plates. In all 60
isolates of Pseudomonas spp. proteolytic and lipolytic activity from milk has been
demonstrated. Isolates of Pseudomonas spp. originating from milk created a biofilm.
Of the 60 isolates, 54 (90%) produced a biofilm of varying intensity, while 6 (10%)
isolates did not produce a biofilm at all. The largest number, 42 (70%) of isolates of
Pseudomonas spp. can be classified into medium-adherent isolates, 10 (16,67%)
isolates as weakly-adherent and only 2 (3,33%) isolates as strongly-adherent isolates.
Isolates of Pseudomonas spp. from milk are good producers of biofilm,
proteolytes and lipolytes so that they can cause rotten milk, and their finding is a
consequence of contamination and poor hygiene conditions during the process of
obtaining milk.",
publisher = "University PIM, Banja Luka, Republic of Srpska, B&H",
journal = "X International Conference on Social and Technological Development",
title = "Characteristics of pseudomonas spp. from raw milk",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2312"
}
