TY  - JOUR
AU  - Debeljak-Martačić, Jasmina
AU  - Francuski, Jelena
AU  - Lužajić, Tijana
AU  - Vuković, Nemanja
AU  - Mojsilović, Slavko
AU  - Drndarević, Neda
AU  - Petakov, Marijana
AU  - Glibetić, Marija
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Todorović, Vera
AU  - Kovačević-Filipović, Milica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1145
AB  - Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies.
AB  - Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Characterization of deciduous teeth stem cells isolated from crown dental pulp
T1  - Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece
VL  - 71
IS  - 8
SP  - 735
EP  - 741
DO  - 10.2298/VSP1408735D
ER  - 

@article{
author = "Debeljak-Martačić, Jasmina and Francuski, Jelena and Lužajić, Tijana and Vuković, Nemanja and Mojsilović, Slavko and Drndarević, Neda and Petakov, Marijana and Glibetić, Marija and Marković, Danica and Radovanović, Anita and Todorović, Vera and Kovačević-Filipović, Milica",
year = "2014",
abstract = "Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies., Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Characterization of deciduous teeth stem cells isolated from crown dental pulp, Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece",
volume = "71",
number = "8",
pages = "735-741",
doi = "10.2298/VSP1408735D"
}

Debeljak-Martačić, J., Francuski, J., Lužajić, T., Vuković, N., Mojsilović, S., Drndarević, N., Petakov, M., Glibetić, M., Marković, D., Radovanović, A., Todorović, V.,& Kovačević-Filipović, M.. (2014). Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(8), 735-741.
https://doi.org/10.2298/VSP1408735D

Debeljak-Martačić J, Francuski J, Lužajić T, Vuković N, Mojsilović S, Drndarević N, Petakov M, Glibetić M, Marković D, Radovanović A, Todorović V, Kovačević-Filipović M. Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled. 2014;71(8):735-741.
doi:10.2298/VSP1408735D .

Debeljak-Martačić, Jasmina, Francuski, Jelena, Lužajić, Tijana, Vuković, Nemanja, Mojsilović, Slavko, Drndarević, Neda, Petakov, Marijana, Glibetić, Marija, Marković, Danica, Radovanović, Anita, Todorović, Vera, Kovačević-Filipović, Milica, "Characterization of deciduous teeth stem cells isolated from crown dental pulp" in Vojnosanitetski pregled, 71, no. 8 (2014):735-741,
https://doi.org/10.2298/VSP1408735D . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Okić, Ivana
AU  - Petrićević, Tanja
AU  - Mojsilović, Slavko
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Milenković, P.
AU  - Petakov, Marijana
AU  - Radovanović, Anita
AU  - Božić, Tatjana
AU  - Ivanović, Z.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/661
AB  - Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia.
AB  - Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cultivation of hamster bone marrow haematopoietic stem and progenitor cells
T1  - Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka
VL  - 60
IS  - 1
SP  - 3
EP  - 14
DO  - 10.2298/AVB1001003K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Okić, Ivana and Petrićević, Tanja and Mojsilović, Slavko and Krstić, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Milenković, P. and Petakov, Marijana and Radovanović, Anita and Božić, Tatjana and Ivanović, Z.",
year = "2010",
abstract = "Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia., Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells, Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka",
volume = "60",
number = "1",
pages = "3-14",
doi = "10.2298/AVB1001003K"
}

Kovačević-Filipović, M., Okić, I., Petrićević, T., Mojsilović, S., Krstić, A., Jovčić, G., Bugarski, D., Milenković, P., Petakov, M., Radovanović, A., Božić, T.,& Ivanović, Z.. (2010). Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1), 3-14.
https://doi.org/10.2298/AVB1001003K

Kovačević-Filipović M, Okić I, Petrićević T, Mojsilović S, Krstić A, Jovčić G, Bugarski D, Milenković P, Petakov M, Radovanović A, Božić T, Ivanović Z. Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd. 2010;60(1):3-14.
doi:10.2298/AVB1001003K .

Kovačević-Filipović, Milica, Okić, Ivana, Petrićević, Tanja, Mojsilović, Slavko, Krstić, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Milenković, P., Petakov, Marijana, Radovanović, Anita, Božić, Tatjana, Ivanović, Z., "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells" in Acta Veterinaria-Beograd, 60, no. 1 (2010):3-14,
https://doi.org/10.2298/AVB1001003K . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Petakov, Marijana
AU  - Hermitte, Francis
AU  - Debeissat, Christelle
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Biligarski, Dijana
AU  - Lafarge, Xavier
AU  - Milenković, Pavle
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/458
AB  - Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.
PB  - Wiley-Liss, Hoboken
T2  - Journal of Cellular Physiology
T1  - Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
VL  - 212
IS  - 1
SP  - 68
EP  - 75
DO  - 10.1002/jcp.21003
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Petakov, Marijana and Hermitte, Francis and Debeissat, Christelle and Krstić, Aleksandra and Jovčić, Gordana and Biligarski, Dijana and Lafarge, Xavier and Milenković, Pavle and Praloran, Vincent and Ivanović, Zoran",
year = "2007",
abstract = "Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.",
publisher = "Wiley-Liss, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)",
volume = "212",
number = "1",
pages = "68-75",
doi = "10.1002/jcp.21003"
}

Kovačević-Filipović, M., Petakov, M., Hermitte, F., Debeissat, C., Krstić, A., Jovčić, G., Biligarski, D., Lafarge, X., Milenković, P., Praloran, V.,& Ivanović, Z.. (2007). Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology
Wiley-Liss, Hoboken., 212(1), 68-75.
https://doi.org/10.1002/jcp.21003

Kovačević-Filipović M, Petakov M, Hermitte F, Debeissat C, Krstić A, Jovčić G, Biligarski D, Lafarge X, Milenković P, Praloran V, Ivanović Z. Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology. 2007;212(1):68-75.
doi:10.1002/jcp.21003 .

Kovačević-Filipović, Milica, Petakov, Marijana, Hermitte, Francis, Debeissat, Christelle, Krstić, Aleksandra, Jovčić, Gordana, Biligarski, Dijana, Lafarge, Xavier, Milenković, Pavle, Praloran, Vincent, Ivanović, Zoran, "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)" in Journal of Cellular Physiology, 212, no. 1 (2007):68-75,
https://doi.org/10.1002/jcp.21003 . .

TY  - JOUR
AU  - Ilić, Vesna
AU  - Petakov, Marijana
AU  - Stojanović, Nevenka
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Grbović, Tatjana
AU  - Božić, Tatjana
AU  - Kovačević-Filipović, Milica
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/385
AB  - Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment.
AB  - Određivanje statusa gvožđa u organizmu jedinke posle primene određenog preparata ovog mikroelementa moguće je utvrditi određivanjem njegove koncentracije u serumu i zasićenja transferina (Trf) gvožđem. U kliničkoj i eksperimentalnoj praksi složeno određivanje koncentracije Trf zamenjeno je jednostavnim određ ivanjem ukupnog serumskog kapaciteta za vezivanje gvožđa (TIBC). Cilj ovog rada je bio da se na modelu porasta serumskog Fe po i.m. aplikaciji Fe-dextrana novorođenoj prasadi, utvrdi odnos TIBC i Trf po aplikaciji ovog mikroelementa, kako bi se utvrdilo da li visoke doze gvožđa u serumu utiču na vrednost TIBC kao mere za određivanje koncentracije Trf. Vrednosti za serumsko Fe, TIBC i Trf poređene su između grupe životinja koja je odmah po rođenju dobila 150 mg Fe-dextrana i kontrolne grupe u kojoj životoinje nisu tretirane Fe-dextranom. Krv je uzorkovana pre aplikacije Fe-dextrana, drugog, osmog i dvanaestog dana po aplikaciji preparata gvožđa. Koncentracija gvožđa u serumu i TIBC su određivani standarnim kliničkim biohemijskim analizama, dok je koncenracija Trf određena denzitometrijom elektroforetskih traka. Dobijeni rezultati ukazuju da bez obzira na primenu preparata Fe, TIBC nije adekvatna mera za Trf kod novorođene prasadi u prva tri dana po rođenju. Osmog dana života prasadi, utvrđen je visoki stepen korelacije ova dva parametra kod životinja koje nisu bile tretirane, dok je kod tretiranih jedinki taj stepen korelacije postignut dvanaestog dana. Na osnovu izloženih rezultata se može zaključiti da se kod novorođene prasadi TIBC može koristiti kao mera za Trf tek 12 dana nakon i.m. tretmana Fe-dextranom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran
T1  - Odnos između totalnog kapaciteta za vezivanje gvožđa i koncentracije transferina kod novorođene prasadi tretirane gvožđe-dekstranom
VL  - 56
IS  - 2-3
SP  - 235
EP  - 242
DO  - 10.2298/AVB0603235I
ER  - 

@article{
author = "Ilić, Vesna and Petakov, Marijana and Stojanović, Nevenka and Jovčić, Gordana and Bugarski, Diana and Grbović, Tatjana and Božić, Tatjana and Kovačević-Filipović, Milica",
year = "2006",
abstract = "Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment., Određivanje statusa gvožđa u organizmu jedinke posle primene određenog preparata ovog mikroelementa moguće je utvrditi određivanjem njegove koncentracije u serumu i zasićenja transferina (Trf) gvožđem. U kliničkoj i eksperimentalnoj praksi složeno određivanje koncentracije Trf zamenjeno je jednostavnim određ ivanjem ukupnog serumskog kapaciteta za vezivanje gvožđa (TIBC). Cilj ovog rada je bio da se na modelu porasta serumskog Fe po i.m. aplikaciji Fe-dextrana novorođenoj prasadi, utvrdi odnos TIBC i Trf po aplikaciji ovog mikroelementa, kako bi se utvrdilo da li visoke doze gvožđa u serumu utiču na vrednost TIBC kao mere za određivanje koncentracije Trf. Vrednosti za serumsko Fe, TIBC i Trf poređene su između grupe životinja koja je odmah po rođenju dobila 150 mg Fe-dextrana i kontrolne grupe u kojoj životoinje nisu tretirane Fe-dextranom. Krv je uzorkovana pre aplikacije Fe-dextrana, drugog, osmog i dvanaestog dana po aplikaciji preparata gvožđa. Koncentracija gvožđa u serumu i TIBC su određivani standarnim kliničkim biohemijskim analizama, dok je koncenracija Trf određena denzitometrijom elektroforetskih traka. Dobijeni rezultati ukazuju da bez obzira na primenu preparata Fe, TIBC nije adekvatna mera za Trf kod novorođene prasadi u prva tri dana po rođenju. Osmog dana života prasadi, utvrđen je visoki stepen korelacije ova dva parametra kod životinja koje nisu bile tretirane, dok je kod tretiranih jedinki taj stepen korelacije postignut dvanaestog dana. Na osnovu izloženih rezultata se može zaključiti da se kod novorođene prasadi TIBC može koristiti kao mera za Trf tek 12 dana nakon i.m. tretmana Fe-dextranom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran, Odnos između totalnog kapaciteta za vezivanje gvožđa i koncentracije transferina kod novorođene prasadi tretirane gvožđe-dekstranom",
volume = "56",
number = "2-3",
pages = "235-242",
doi = "10.2298/AVB0603235I"
}

Ilić, V., Petakov, M., Stojanović, N., Jovčić, G., Bugarski, D., Grbović, T., Božić, T.,& Kovačević-Filipović, M.. (2006). Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 235-242.
https://doi.org/10.2298/AVB0603235I

Ilić V, Petakov M, Stojanović N, Jovčić G, Bugarski D, Grbović T, Božić T, Kovačević-Filipović M. Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran. in Acta Veterinaria-Beograd. 2006;56(2-3):235-242.
doi:10.2298/AVB0603235I .

Ilić, Vesna, Petakov, Marijana, Stojanović, Nevenka, Jovčić, Gordana, Bugarski, Diana, Grbović, Tatjana, Božić, Tatjana, Kovačević-Filipović, Milica, "Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):235-242,
https://doi.org/10.2298/AVB0603235I . .

TY  - JOUR
AU  - Bugarski, Diana
AU  - Jovčić, Gordana
AU  - Katić-Radivojević, Sofija
AU  - Petakov, Marijana
AU  - Krstić, Aleksandra
AU  - Stojanović, Nevenka
AU  - Milenković, Pavle
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/431
AB  - Pinworm parasites commonly infect laboratory mice with high prevalence even in well-managed animal colonies. Although often considered as irrelevant, these parasites if undetected may significantly interfere with the experimental settings and alter the interpretation of final results. There are a few reports documenting the effects of pinworms on research and the effects of pinworms on the host hematopoiesis have not yet been investigated. In this study we examined the changes within various hematopoietic cell lineages in the bone marrow, spleen, peripheral blood and peritoneal space during naturally acquired Syphacia obvelata infection in inbred CBA mice. The data obtained showed significant hematopoietic alterations, characterized by increased myelopoiesis and erythropoiesis in S. obvelata-infected animals. In order to additionally evaluate if this pinworm infection modifies hematopoietic cells reactivity, we examined the effect of murine interleukin-17, T cell-derived cytokine implicated in the regulation of hematopoiesis and inflammation, on the growth of bone marrow progenitor cells and demonstrated that bone marrow myeloid and erythroid progenitors from S. obvelata-infected mice displayed altered sensitivity to IL-17 when compared to non-infected controls. Taken together the alterations presented pointed out that this rodent pinworm is an important environmental agent that might significantly modify the hosts hematopoietic response, and therefore interfere with the experimental settings and alter the interpretation of the final results. However, the results obtained also contributed new data concerning the activity of IL-17 on bone marrow hematopoietic cells, supporting our previous reports that depending on physiological/pathological status of the organism IL-17 exerts differential effects on the growth of progenitor-cells.
PB  - Elsevier Ireland Ltd, Clare
T2  - Parasitology International
T1  - Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata-infected mice
VL  - 55
IS  - 2
SP  - 91
EP  - 97
DO  - 10.1016/j.parint.2005.10.005
ER  - 

@article{
author = "Bugarski, Diana and Jovčić, Gordana and Katić-Radivojević, Sofija and Petakov, Marijana and Krstić, Aleksandra and Stojanović, Nevenka and Milenković, Pavle",
year = "2006",
abstract = "Pinworm parasites commonly infect laboratory mice with high prevalence even in well-managed animal colonies. Although often considered as irrelevant, these parasites if undetected may significantly interfere with the experimental settings and alter the interpretation of final results. There are a few reports documenting the effects of pinworms on research and the effects of pinworms on the host hematopoiesis have not yet been investigated. In this study we examined the changes within various hematopoietic cell lineages in the bone marrow, spleen, peripheral blood and peritoneal space during naturally acquired Syphacia obvelata infection in inbred CBA mice. The data obtained showed significant hematopoietic alterations, characterized by increased myelopoiesis and erythropoiesis in S. obvelata-infected animals. In order to additionally evaluate if this pinworm infection modifies hematopoietic cells reactivity, we examined the effect of murine interleukin-17, T cell-derived cytokine implicated in the regulation of hematopoiesis and inflammation, on the growth of bone marrow progenitor cells and demonstrated that bone marrow myeloid and erythroid progenitors from S. obvelata-infected mice displayed altered sensitivity to IL-17 when compared to non-infected controls. Taken together the alterations presented pointed out that this rodent pinworm is an important environmental agent that might significantly modify the hosts hematopoietic response, and therefore interfere with the experimental settings and alter the interpretation of the final results. However, the results obtained also contributed new data concerning the activity of IL-17 on bone marrow hematopoietic cells, supporting our previous reports that depending on physiological/pathological status of the organism IL-17 exerts differential effects on the growth of progenitor-cells.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Parasitology International",
title = "Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata-infected mice",
volume = "55",
number = "2",
pages = "91-97",
doi = "10.1016/j.parint.2005.10.005"
}

Bugarski, D., Jovčić, G., Katić-Radivojević, S., Petakov, M., Krstić, A., Stojanović, N.,& Milenković, P.. (2006). Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata-infected mice. in Parasitology International
Elsevier Ireland Ltd, Clare., 55(2), 91-97.
https://doi.org/10.1016/j.parint.2005.10.005

Bugarski D, Jovčić G, Katić-Radivojević S, Petakov M, Krstić A, Stojanović N, Milenković P. Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata-infected mice. in Parasitology International. 2006;55(2):91-97.
doi:10.1016/j.parint.2005.10.005 .

Bugarski, Diana, Jovčić, Gordana, Katić-Radivojević, Sofija, Petakov, Marijana, Krstić, Aleksandra, Stojanović, Nevenka, Milenković, Pavle, "Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata-infected mice" in Parasitology International, 55, no. 2 (2006):91-97,
https://doi.org/10.1016/j.parint.2005.10.005 . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Stevanović, Jelka
AU  - Božić, Tatjana
AU  - Petakov, Marijana
PY  - 2004
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/276
AB  - The potential toxic effect of toluene on erythropoiesis was investigated during the enhanced erythropoietic activity provoked by application of propylene glycol (known as a mild hemolytic agent) in adult female Wistar rats. The animals were treated daily for 3, 7 or 11 days, with an intraperitoneal dose of toluene dissolved in propylene glycol (T+PG) or propylene glycol (PG) alone. The effects of T+PG and PG on some hematological parameters in peripheral blood and bone marrow were evaluated at the time of sacrifice. The number of red blood cells (RBC), reticulocytes (Rt) hematocrit values (PCV), and hemoglobin concentration (Hb) were determined in peripheral blood samples using standard laboratory procedures. Total bone marrow nucleated cells (TBMNC) were counted and the myelogram was analyzed as well. The number of early bone marrow erythroid progenitor cells-Burst Forming Unit-Erythroid (BFU-E) was assessed using a colony forming assay on methylcellulose. Toluene dissolved in PG induced a decrease in RBC number PCV and Hb concentration and an increase within the bone marrow BFU-E and precursor erythroid cell compartments, as well as the percentage of peripheral blood Rt, indicating enhanced erythropoietic activity. Very similar changes occurred when PG was administered alone. Therefore, it can be assumed that short-term application of a low dose of toluene (3 mg/kg) dissolved in PG did not have a toxic effect during PG induced enhanced erythropoietic activity.
AB  - U ovom radu su prikazani rezultati ispitivanja mogućeg toksičnog uticaja toluena na eritropoezu tokom pojačane eritropoetske aktivnosti, izazvane aplikacijom blagog hemolitičkog agensa propilen glikola, kod odraslih ženki pacova Wistar soja. Eksperimentalnim životinjama je jedanput dnevno, tokom 3 7 i 11 dana, intraperitonealno aplikovan toluen rastvoren u propilen glikolu (T+PG), odnosno samo propilen glikol (PG). Efekti T+PG i PG na hematološke parametre periferne krvi i kostne srži su određivani u vreme žrtvovanja životinja. Broj eritrocita, retikulocita, hematokritska vrednost i koncentracija hemoglobina su određivani u perifernoj krvi standardnim laboratorijskim procedurama. U kostnoj srži je određivan ukupan broj ćelija sa jedrom i ispitan odnos ćelija prekursora mijeloidne i eritroidne loze. Broj ranih opredeljenih matičnih ćelija za eritrocitopoezu (BFU-E) određen je in vitro, na osnovu broja kolonija formiranih iz ćelija kostne srži na polučvrstoj podlozi od metil celuloze. Toluen rastvoren u propilen glikolu je u perifernoj krvi izazvao pad broja eritrocita, pad hematokritske vrednosti smanjenje koncentracije hemoglobina, a doveo je do porasta udela retikulocita. U kostnoj srži je došlo do porasta broja BFU-E, kao i eritrocitnih prekursora, što ukazuje na pojačanu eritropoetsku aktivnost. Ispitivani parametri se nisu značajno razlikovali prilikom aplikacije samog propilen glikola u odnosu na vrednosti parametara dobijenih prilikom aplikacije toluena rastvorenog u propilen glikolu. Zbog toga možemo da pretpostavimo da kratkotrajna aplikacija niske doze toluena (3 mg/kg) nema toksičan efekat na eritropoezu tokom pojačane eritropoetske aktivnosti izazvane aplikacijom propilen glikola.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of intraperitoneal application of toluene dissolved in propylene glycol on erythropoiesis in Wistar rats
T1  - Uticaj intraperitonealne aplikacije toluena rastvorenog u propilen glikolu na eritropoezu pacova wistar soja
VL  - 54
IS  - 5-6
SP  - 357
EP  - 367
DO  - 10.2298/AVB0406357K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Stevanović, Jelka and Božić, Tatjana and Petakov, Marijana",
year = "2004",
abstract = "The potential toxic effect of toluene on erythropoiesis was investigated during the enhanced erythropoietic activity provoked by application of propylene glycol (known as a mild hemolytic agent) in adult female Wistar rats. The animals were treated daily for 3, 7 or 11 days, with an intraperitoneal dose of toluene dissolved in propylene glycol (T+PG) or propylene glycol (PG) alone. The effects of T+PG and PG on some hematological parameters in peripheral blood and bone marrow were evaluated at the time of sacrifice. The number of red blood cells (RBC), reticulocytes (Rt) hematocrit values (PCV), and hemoglobin concentration (Hb) were determined in peripheral blood samples using standard laboratory procedures. Total bone marrow nucleated cells (TBMNC) were counted and the myelogram was analyzed as well. The number of early bone marrow erythroid progenitor cells-Burst Forming Unit-Erythroid (BFU-E) was assessed using a colony forming assay on methylcellulose. Toluene dissolved in PG induced a decrease in RBC number PCV and Hb concentration and an increase within the bone marrow BFU-E and precursor erythroid cell compartments, as well as the percentage of peripheral blood Rt, indicating enhanced erythropoietic activity. Very similar changes occurred when PG was administered alone. Therefore, it can be assumed that short-term application of a low dose of toluene (3 mg/kg) dissolved in PG did not have a toxic effect during PG induced enhanced erythropoietic activity., U ovom radu su prikazani rezultati ispitivanja mogućeg toksičnog uticaja toluena na eritropoezu tokom pojačane eritropoetske aktivnosti, izazvane aplikacijom blagog hemolitičkog agensa propilen glikola, kod odraslih ženki pacova Wistar soja. Eksperimentalnim životinjama je jedanput dnevno, tokom 3 7 i 11 dana, intraperitonealno aplikovan toluen rastvoren u propilen glikolu (T+PG), odnosno samo propilen glikol (PG). Efekti T+PG i PG na hematološke parametre periferne krvi i kostne srži su određivani u vreme žrtvovanja životinja. Broj eritrocita, retikulocita, hematokritska vrednost i koncentracija hemoglobina su određivani u perifernoj krvi standardnim laboratorijskim procedurama. U kostnoj srži je određivan ukupan broj ćelija sa jedrom i ispitan odnos ćelija prekursora mijeloidne i eritroidne loze. Broj ranih opredeljenih matičnih ćelija za eritrocitopoezu (BFU-E) određen je in vitro, na osnovu broja kolonija formiranih iz ćelija kostne srži na polučvrstoj podlozi od metil celuloze. Toluen rastvoren u propilen glikolu je u perifernoj krvi izazvao pad broja eritrocita, pad hematokritske vrednosti smanjenje koncentracije hemoglobina, a doveo je do porasta udela retikulocita. U kostnoj srži je došlo do porasta broja BFU-E, kao i eritrocitnih prekursora, što ukazuje na pojačanu eritropoetsku aktivnost. Ispitivani parametri se nisu značajno razlikovali prilikom aplikacije samog propilen glikola u odnosu na vrednosti parametara dobijenih prilikom aplikacije toluena rastvorenog u propilen glikolu. Zbog toga možemo da pretpostavimo da kratkotrajna aplikacija niske doze toluena (3 mg/kg) nema toksičan efekat na eritropoezu tokom pojačane eritropoetske aktivnosti izazvane aplikacijom propilen glikola.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of intraperitoneal application of toluene dissolved in propylene glycol on erythropoiesis in Wistar rats, Uticaj intraperitonealne aplikacije toluena rastvorenog u propilen glikolu na eritropoezu pacova wistar soja",
volume = "54",
number = "5-6",
pages = "357-367",
doi = "10.2298/AVB0406357K"
}

Kovačević-Filipović, M., Stevanović, J., Božić, T.,& Petakov, M.. (2004). Effects of intraperitoneal application of toluene dissolved in propylene glycol on erythropoiesis in Wistar rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 54(5-6), 357-367.
https://doi.org/10.2298/AVB0406357K

Kovačević-Filipović M, Stevanović J, Božić T, Petakov M. Effects of intraperitoneal application of toluene dissolved in propylene glycol on erythropoiesis in Wistar rats. in Acta Veterinaria-Beograd. 2004;54(5-6):357-367.
doi:10.2298/AVB0406357K .

Kovačević-Filipović, Milica, Stevanović, Jelka, Božić, Tatjana, Petakov, Marijana, "Effects of intraperitoneal application of toluene dissolved in propylene glycol on erythropoiesis in Wistar rats" in Acta Veterinaria-Beograd, 54, no. 5-6 (2004):357-367,
https://doi.org/10.2298/AVB0406357K . .

TY  - JOUR
AU  - Božić, Tatjana
AU  - Stevanović, Jelka
AU  - Kovačević, Milica
AU  - Jović, Slavoljub
AU  - Lukić, Sanja
AU  - Petakov, Marijana
AU  - Borozan, Sunčica
AU  - Mijačević, Zora
AU  - Knežević, Milijana
AU  - Bulajić, Snežana
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/236
AB  - The influence of toluene exposure on some biochemical and hematological parameters was investigated in adult female Wistar rats. The animals were subjected to intraperitoneal administration of toluene diluted in propylene glycol and the diluent alone for 3, 7 and 11 consecutive days at the same time intervals. The effects of toluene and propylene glycol were evaluated biochemically by determining the plasma concentrations of total proteins albumin and ceruloplasmin (Cp) together with erythrocyte malondialdehyde (MDA), and hematological indices from peripheral blood and bone marrow. The biochemical acute phase response was manifested by an elevated Cp concentration in all experimental animals. The markedly enhanced MDA concentration and statistically significant decrease in albumin level in toluene treated rats, indicated damage, to blood vessel endothelia. Alterations of leukocytes of peripheral blood and bone marrow (BM) granulocytic-monocytic progenitor cells were typical of an inflammatory response, with stimulation of granulo-monocytopoiesis. Therefore, it can be assumed that both toluene and propylene glycol mediated sterile peritonitis and oxidative stress injury, with changes intensified by toluene action.
AB  - U ovom ogledu je ispitivan efekat toluena na biohemijske i hematološke parametre odraslih ženki Wistar pacova. Životinjama je intraperitonealno davan rastvor toluena u propilen glikolu, kao i propilen glikol, jednom dnevno u trajanju od 3, 7 i 11 dana. Efekti davanih rastvora odredjivani su biohemijskim i hematološkim ispitivanjima plazme, krvi i punktata kostne srži. U plazmi pacova određivani su koncentracije ukupnih proteina, albumina kao i koncentracija eritrocitnog malondialdehida (RBC MDA). Biohemijske promene manifestovale su se hipoalbuminemijom, porastom koncentracije ceruloplazmina i visokom koncentracijom RBC MDA. Ovi nalazi ukazuju na oštećenje endotela krvnih sudova praćeno odgovorom akutne faze. Promene u broju leukocita periferne krvi kao i povećanje broja granulocitno-monocitnih (CFU-GM) progenitora kostne srži takođe su ukazali na tipičan proinflamatorni odgovor. Naši rezultati potvrđuju da toluen izaziva oksidativni stres i sterilni akutni peritonitis. Promene izazvane slobodnim radikalima kiseonika utiču i na odgovor progenitora kostne srži, pojavu koja do sada nije bila opisana.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Toluene mediated oxidative stress and granulo-monocytopoiesis
T1  - Toluenom posredovani oksidativni stres i granulo-monocitopoeza
VL  - 53
IS  - 4
SP  - 201
EP  - 210
DO  - 10.2298/AVB0304201B
ER  - 

@article{
author = "Božić, Tatjana and Stevanović, Jelka and Kovačević, Milica and Jović, Slavoljub and Lukić, Sanja and Petakov, Marijana and Borozan, Sunčica and Mijačević, Zora and Knežević, Milijana and Bulajić, Snežana",
year = "2003",
abstract = "The influence of toluene exposure on some biochemical and hematological parameters was investigated in adult female Wistar rats. The animals were subjected to intraperitoneal administration of toluene diluted in propylene glycol and the diluent alone for 3, 7 and 11 consecutive days at the same time intervals. The effects of toluene and propylene glycol were evaluated biochemically by determining the plasma concentrations of total proteins albumin and ceruloplasmin (Cp) together with erythrocyte malondialdehyde (MDA), and hematological indices from peripheral blood and bone marrow. The biochemical acute phase response was manifested by an elevated Cp concentration in all experimental animals. The markedly enhanced MDA concentration and statistically significant decrease in albumin level in toluene treated rats, indicated damage, to blood vessel endothelia. Alterations of leukocytes of peripheral blood and bone marrow (BM) granulocytic-monocytic progenitor cells were typical of an inflammatory response, with stimulation of granulo-monocytopoiesis. Therefore, it can be assumed that both toluene and propylene glycol mediated sterile peritonitis and oxidative stress injury, with changes intensified by toluene action., U ovom ogledu je ispitivan efekat toluena na biohemijske i hematološke parametre odraslih ženki Wistar pacova. Životinjama je intraperitonealno davan rastvor toluena u propilen glikolu, kao i propilen glikol, jednom dnevno u trajanju od 3, 7 i 11 dana. Efekti davanih rastvora odredjivani su biohemijskim i hematološkim ispitivanjima plazme, krvi i punktata kostne srži. U plazmi pacova određivani su koncentracije ukupnih proteina, albumina kao i koncentracija eritrocitnog malondialdehida (RBC MDA). Biohemijske promene manifestovale su se hipoalbuminemijom, porastom koncentracije ceruloplazmina i visokom koncentracijom RBC MDA. Ovi nalazi ukazuju na oštećenje endotela krvnih sudova praćeno odgovorom akutne faze. Promene u broju leukocita periferne krvi kao i povećanje broja granulocitno-monocitnih (CFU-GM) progenitora kostne srži takođe su ukazali na tipičan proinflamatorni odgovor. Naši rezultati potvrđuju da toluen izaziva oksidativni stres i sterilni akutni peritonitis. Promene izazvane slobodnim radikalima kiseonika utiču i na odgovor progenitora kostne srži, pojavu koja do sada nije bila opisana.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Toluene mediated oxidative stress and granulo-monocytopoiesis, Toluenom posredovani oksidativni stres i granulo-monocitopoeza",
volume = "53",
number = "4",
pages = "201-210",
doi = "10.2298/AVB0304201B"
}

Božić, T., Stevanović, J., Kovačević, M., Jović, S., Lukić, S., Petakov, M., Borozan, S., Mijačević, Z., Knežević, M.,& Bulajić, S.. (2003). Toluene mediated oxidative stress and granulo-monocytopoiesis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(4), 201-210.
https://doi.org/10.2298/AVB0304201B

Božić T, Stevanović J, Kovačević M, Jović S, Lukić S, Petakov M, Borozan S, Mijačević Z, Knežević M, Bulajić S. Toluene mediated oxidative stress and granulo-monocytopoiesis. in Acta Veterinaria-Beograd. 2003;53(4):201-210.
doi:10.2298/AVB0304201B .

Božić, Tatjana, Stevanović, Jelka, Kovačević, Milica, Jović, Slavoljub, Lukić, Sanja, Petakov, Marijana, Borozan, Sunčica, Mijačević, Zora, Knežević, Milijana, Bulajić, Snežana, "Toluene mediated oxidative stress and granulo-monocytopoiesis" in Acta Veterinaria-Beograd, 53, no. 4 (2003):201-210,
https://doi.org/10.2298/AVB0304201B . .

TY  - JOUR
AU  - Kovačević, Milica
AU  - Božić, Tatjana
AU  - Jovčić, Gordana
AU  - Petakov, Marijana
AU  - Bugarski, Diana
AU  - Stanković, Jelena
AU  - Ivanović, Z.
PY  - 2001
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/169
AB  - The pig is widely used as a large animal model for biomedical research and could be an interesting experimental model for studies of the hematopoietic system and its response in physiological and pathological conditions. With the intention of using the pig as a large animal model in hematopoietic research, a clonal assay in methylcellulose was developed and standardized for detection of committed progenitors of the granulocyte-macrophage lineage from adult pig bone marrow and peripheral blood. Progenitor cells were stimulated to proliferate and differentiate in vitro by adding pig leukocyte conditioned medium (LCM) as a source of homologous growth factors. The number of CFU-GM (Colony Forming Unit -Granulocyte-Macrophage) directly depended on the concentration of LCM. The proliferative rate of CFU-GM progenitor cells was determined by the cytosine arabinoside suicide technique. The percentage of bone marrow and peripheral blood CFU-GM cells in S phase of the cell cycle was 34.7% and 22.2%, respectively. The data obtained regarding the number and characteristics of pig bone marrow and peripheral blood CFU-GM confirmed that the organization of the pig CFU-GM progenitor cell compartment is similar and comparable to that in miniature swine, other animal species and humans.
AB  - Svinja je životinja koja se koristi kao model u različitim biomedicinskim istraživanjima, a mogia bi biti i interesantan model u ispitivanju fiziologije i patolofizioloških promena hematopoetskog sistema. U cilju razvoja eksperimentalnog modela svinje u istraživanju hematopoeze, stanadardizovan je esej za odredivanje i karakterizaciju opredeljenih matičnih ćelija granulocitno-monocitne loze iz kostne srži i krvi odrasle svinje. Stimulacija proliferacije i diferencijacije ovih matičnih ćelija postignuta je dodavanjem medijuma kondicioniranog leukocitima (LCM - Leukocyte conditioned medium) bogatog faktorima rasta. Broj CFU-GM (Colony forming unit- granulocyte-macrophage) je direktno zavisio od koncentracije LCM-a. Procenat CFU-GM ćelija u S fazi ćelijskog cikiusa odredjivan je tehnikom 'suicida' korišćenjem citozin arabinozida (Ara-C) i iznosio je 34.7% za CFU-GM iz kostne srži i 22.2% za CFU-GM iz periferne krvi. Podaci dobijeni za broj i karakteristike CFU-GM iz kostne srži i periferne krvi potvrđuju da je ovaj odeljak matičnih ćelija kod odraslih svinja organizovan na isti način kao i kod minijaturnih svinja, drugih vrsta životinja i ljudi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells
T1  - Matične ćelije opredeljene za granulocitno-monocitnu lozu kostne srži i periferne krvi svinja
VL  - 51
IS  - 1
SP  - 15
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_169
ER  - 

@article{
author = "Kovačević, Milica and Božić, Tatjana and Jovčić, Gordana and Petakov, Marijana and Bugarski, Diana and Stanković, Jelena and Ivanović, Z.",
year = "2001",
abstract = "The pig is widely used as a large animal model for biomedical research and could be an interesting experimental model for studies of the hematopoietic system and its response in physiological and pathological conditions. With the intention of using the pig as a large animal model in hematopoietic research, a clonal assay in methylcellulose was developed and standardized for detection of committed progenitors of the granulocyte-macrophage lineage from adult pig bone marrow and peripheral blood. Progenitor cells were stimulated to proliferate and differentiate in vitro by adding pig leukocyte conditioned medium (LCM) as a source of homologous growth factors. The number of CFU-GM (Colony Forming Unit -Granulocyte-Macrophage) directly depended on the concentration of LCM. The proliferative rate of CFU-GM progenitor cells was determined by the cytosine arabinoside suicide technique. The percentage of bone marrow and peripheral blood CFU-GM cells in S phase of the cell cycle was 34.7% and 22.2%, respectively. The data obtained regarding the number and characteristics of pig bone marrow and peripheral blood CFU-GM confirmed that the organization of the pig CFU-GM progenitor cell compartment is similar and comparable to that in miniature swine, other animal species and humans., Svinja je životinja koja se koristi kao model u različitim biomedicinskim istraživanjima, a mogia bi biti i interesantan model u ispitivanju fiziologije i patolofizioloških promena hematopoetskog sistema. U cilju razvoja eksperimentalnog modela svinje u istraživanju hematopoeze, stanadardizovan je esej za odredivanje i karakterizaciju opredeljenih matičnih ćelija granulocitno-monocitne loze iz kostne srži i krvi odrasle svinje. Stimulacija proliferacije i diferencijacije ovih matičnih ćelija postignuta je dodavanjem medijuma kondicioniranog leukocitima (LCM - Leukocyte conditioned medium) bogatog faktorima rasta. Broj CFU-GM (Colony forming unit- granulocyte-macrophage) je direktno zavisio od koncentracije LCM-a. Procenat CFU-GM ćelija u S fazi ćelijskog cikiusa odredjivan je tehnikom 'suicida' korišćenjem citozin arabinozida (Ara-C) i iznosio je 34.7% za CFU-GM iz kostne srži i 22.2% za CFU-GM iz periferne krvi. Podaci dobijeni za broj i karakteristike CFU-GM iz kostne srži i periferne krvi potvrđuju da je ovaj odeljak matičnih ćelija kod odraslih svinja organizovan na isti način kao i kod minijaturnih svinja, drugih vrsta životinja i ljudi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells, Matične ćelije opredeljene za granulocitno-monocitnu lozu kostne srži i periferne krvi svinja",
volume = "51",
number = "1",
pages = "15-26",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_169"
}

Kovačević, M., Božić, T., Jovčić, G., Petakov, M., Bugarski, D., Stanković, J.,& Ivanović, Z.. (2001). Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 51(1), 15-26.
https://hdl.handle.net/21.15107/rcub_veterinar_169

Kovačević M, Božić T, Jovčić G, Petakov M, Bugarski D, Stanković J, Ivanović Z. Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells. in Acta Veterinaria-Beograd. 2001;51(1):15-26.
https://hdl.handle.net/21.15107/rcub_veterinar_169 .

Kovačević, Milica, Božić, Tatjana, Jovčić, Gordana, Petakov, Marijana, Bugarski, Diana, Stanković, Jelena, Ivanović, Z., "Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells" in Acta Veterinaria-Beograd, 51, no. 1 (2001):15-26,
https://hdl.handle.net/21.15107/rcub_veterinar_169 .

TY  - JOUR
AU  - Kovačević, Milica
AU  - Božić, Tatjana
AU  - Pavlović, V.
AU  - Petakov, Marijana
AU  - Bugarski, Diana
AU  - Jovčić, Gordana
AU  - Ivanović, Z.
PY  - 2000
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/146
AB  - The proliferative rate of hemopoietic progenitor cells, i.e. the percentage of cells in the synthetic (S) phase of the cell cycle is a very sensitive parameter for detecting the steady-state misbalance arising in response to stimulatory or inhibitory molecules, or in postmyeloablative repopulation of hemopoiesis. This parameter is also predictive for the sensitivity of these cells to irradiation injury and specific cytotoxic drugs. In spite of the fact that miniature inbred and domestic pig breeds have been used as large animal models in hemopoietic research and in experimental bone marrow transplantation, data concerning the proliferative rate of porcine progenitor cells are still lacking. The aim of this study was to examine the steady-state proliferative rate of bone marrow and peripheral blood erythroid progenitor cells of adult pigs. The percentage of cells in S phase of the cell cycle of both immature, BFU-E (Burst Forming Unit-Erythroid) and mature, CFU-E (Colony Forming Unit-Erythmid) erythroid progenitor cells was determined by the suicide technique based on the proportion of these cells killed after in vitro treatment of the cells with cytosine arabinoside (Ara-C). The results revealed different relative numbers of immature and mature erythroid progenitor cells in S phase of the cell cycle in the bone marrow, namely, 33.3% for BFU-E and 55.1% for CFU-E. In peripheral blood the proliferative rate of BFU-E progenitors was 26.3%. The data obtained show that the proliferative rate of porcine erythroid progenitor cells is similar to the values determined for other mammalian species.
AB  - Proliferativna aktivnost opredeljenih matičnih ćelija hematopoeze, odnosno procenat ovih ćelija u S (sintetskoj) fazi ćelijskog ciklusa je veoma osetljiv parametar za određivanje poremećajafizioloških vrednosti koje nastaju dejstvom stimulatomih ili inhibitornih molekula, ili kod repopulacije hematopoeze posle mijeloablacije. Ovaj parametar je takođe vredan u ocenjivanju osetljivosti matičnih ćelija na radijacione povrede ili određene citotoksične lekove. Uprkos činjenici da su kao eksperimentalni modeli u hematopoetskim istraživanjima i u eksperimentalnoj transplantaciji kostne srži korišćeni kako visoko-srodni sojevi minijaturnih, tako i industrijske rase svinja, literaturni podaci o proliferativnoj aktivnosti opredeljenih matičnih ćelija još uvek nedostaju. Cilj ovog istraživanja je bio da se odrede proliferativne aktivnosti opredeljenih matičnih ćelija eritropoeze iz kostne srži i periferne krvi odraslih svinja u fiziološkim uslovima. Procenat opredeljenih matičnih ćelija za eritropoezu, BFU-E (Burst forming unit-erythroid) i CFU-E (Colony forming unit-erythroid) u S fazi ćelijskog ciklusa je određivan tehnikom 'suicida'. Metoda se zasniva na in vitro 'ubijanju' ćelija u S fazi ćelijskog ciklusa citozin arabinozidom (Ara-C). Rezultati su pokazali da je procenat ranih i zrelih opredeljenih matičnih ćelija za eritropoezu u S fazi ćelijskog ciklusa različit, i iznosio je 33,3% za BFU-E i 55,1 % za CFU-E ćelije. U perifernoj krvi proliferativna aktivnost BFU-E je iznosila 26,3%. Dobijeni rezultati pokazuju da je proliferativna aktivnost opredeljenih matičnih ćelija eritropoeze kod svinja slična vrednostima dobijenim kod drugih vrsta sisara.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle
T1  - Opredeljene matične ćelije eritropoeze kostne srži i periferne krvi svinja u S fazi ćelijskog ciklusa
VL  - 50
IS  - 4
SP  - 207
EP  - 214
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_146
ER  - 

@article{
author = "Kovačević, Milica and Božić, Tatjana and Pavlović, V. and Petakov, Marijana and Bugarski, Diana and Jovčić, Gordana and Ivanović, Z.",
year = "2000",
abstract = "The proliferative rate of hemopoietic progenitor cells, i.e. the percentage of cells in the synthetic (S) phase of the cell cycle is a very sensitive parameter for detecting the steady-state misbalance arising in response to stimulatory or inhibitory molecules, or in postmyeloablative repopulation of hemopoiesis. This parameter is also predictive for the sensitivity of these cells to irradiation injury and specific cytotoxic drugs. In spite of the fact that miniature inbred and domestic pig breeds have been used as large animal models in hemopoietic research and in experimental bone marrow transplantation, data concerning the proliferative rate of porcine progenitor cells are still lacking. The aim of this study was to examine the steady-state proliferative rate of bone marrow and peripheral blood erythroid progenitor cells of adult pigs. The percentage of cells in S phase of the cell cycle of both immature, BFU-E (Burst Forming Unit-Erythroid) and mature, CFU-E (Colony Forming Unit-Erythmid) erythroid progenitor cells was determined by the suicide technique based on the proportion of these cells killed after in vitro treatment of the cells with cytosine arabinoside (Ara-C). The results revealed different relative numbers of immature and mature erythroid progenitor cells in S phase of the cell cycle in the bone marrow, namely, 33.3% for BFU-E and 55.1% for CFU-E. In peripheral blood the proliferative rate of BFU-E progenitors was 26.3%. The data obtained show that the proliferative rate of porcine erythroid progenitor cells is similar to the values determined for other mammalian species., Proliferativna aktivnost opredeljenih matičnih ćelija hematopoeze, odnosno procenat ovih ćelija u S (sintetskoj) fazi ćelijskog ciklusa je veoma osetljiv parametar za određivanje poremećajafizioloških vrednosti koje nastaju dejstvom stimulatomih ili inhibitornih molekula, ili kod repopulacije hematopoeze posle mijeloablacije. Ovaj parametar je takođe vredan u ocenjivanju osetljivosti matičnih ćelija na radijacione povrede ili određene citotoksične lekove. Uprkos činjenici da su kao eksperimentalni modeli u hematopoetskim istraživanjima i u eksperimentalnoj transplantaciji kostne srži korišćeni kako visoko-srodni sojevi minijaturnih, tako i industrijske rase svinja, literaturni podaci o proliferativnoj aktivnosti opredeljenih matičnih ćelija još uvek nedostaju. Cilj ovog istraživanja je bio da se odrede proliferativne aktivnosti opredeljenih matičnih ćelija eritropoeze iz kostne srži i periferne krvi odraslih svinja u fiziološkim uslovima. Procenat opredeljenih matičnih ćelija za eritropoezu, BFU-E (Burst forming unit-erythroid) i CFU-E (Colony forming unit-erythroid) u S fazi ćelijskog ciklusa je određivan tehnikom 'suicida'. Metoda se zasniva na in vitro 'ubijanju' ćelija u S fazi ćelijskog ciklusa citozin arabinozidom (Ara-C). Rezultati su pokazali da je procenat ranih i zrelih opredeljenih matičnih ćelija za eritropoezu u S fazi ćelijskog ciklusa različit, i iznosio je 33,3% za BFU-E i 55,1 % za CFU-E ćelije. U perifernoj krvi proliferativna aktivnost BFU-E je iznosila 26,3%. Dobijeni rezultati pokazuju da je proliferativna aktivnost opredeljenih matičnih ćelija eritropoeze kod svinja slična vrednostima dobijenim kod drugih vrsta sisara.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle, Opredeljene matične ćelije eritropoeze kostne srži i periferne krvi svinja u S fazi ćelijskog ciklusa",
volume = "50",
number = "4",
pages = "207-214",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_146"
}

Kovačević, M., Božić, T., Pavlović, V., Petakov, M., Bugarski, D., Jovčić, G.,& Ivanović, Z.. (2000). Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 50(4), 207-214.
https://hdl.handle.net/21.15107/rcub_veterinar_146

Kovačević M, Božić T, Pavlović V, Petakov M, Bugarski D, Jovčić G, Ivanović Z. Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle. in Acta Veterinaria-Beograd. 2000;50(4):207-214.
https://hdl.handle.net/21.15107/rcub_veterinar_146 .

Kovačević, Milica, Božić, Tatjana, Pavlović, V., Petakov, Marijana, Bugarski, Diana, Jovčić, Gordana, Ivanović, Z., "Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle" in Acta Veterinaria-Beograd, 50, no. 4 (2000):207-214,
https://hdl.handle.net/21.15107/rcub_veterinar_146 .