TY  - JOUR
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Aksentijević, Ksenija
AU  - Tambur, Zoran
AU  - Rakonjac, Bojan
AU  - Kovačević, Ivana
AU  - Spergser, Joachim
AU  - Loncarić, Igor
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1758
AB  - The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia
VL  - 8
IS  - 1
SP  - 26
DO  - 10.3390/antibiotics8010026
ER  - 

@article{
author = "Ašanin, Jelena and Mišić, Dušan and Aksentijević, Ksenija and Tambur, Zoran and Rakonjac, Bojan and Kovačević, Ivana and Spergser, Joachim and Loncarić, Igor",
year = "2019",
abstract = "The aim of this study was to characterize a collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates of human and animal origin from Serbia. In total, 36 MRSA isolates-30 obtained from humans and six from companion animals-were investigated by PCR for the presence of antibiotic and biocide resistance determinants and virulence genes (PVL-Panton-Valentine leukocidin, ETs-exfoliative toxins, TSST-toxic shock syndrome toxin, SEs-staphylococcal enterotoxins, and MSCRAMMs-microbial surface components recognizing adhesive matrix molecules and biofilm). Isolates were analyzed by staphylococcal cassette chromosome mec (SCCmec), spa, and dru typing, as well as by multiple locus variable number of tandem repeat analyses (MLVA), multilocus sequence typing (MLST), and subsequently, eBURST. The majority of human MRSA isolates were resistant to gentamicin, erythromycin, clindamycin, and ciprofloxacin. Different antibiotic resistance genes were detected: aac-aphD, ant(6 )-Ia, erm(A), erm(B), erm(C), tet(K), tet(M), fexA, and cat(pC221). All isolates were susceptible to teicoplanin and linezolid. SCCmec type III was prevalent in human isolates, while SCCmec elements in animals were mostly nontypeable. t037 was the predominant spa type in human and t242 in animal MRSA isolates. The prevalent dru type was dt11c in human and dt10a in animal MRSA isolates. MRSA isolates exhibited 27 different MLVA types. ST239 was predominant in human, while ST5 was prevalent in canine MRSA isolates. PVL was found in two, while tsst-1 was detected in three human isolates. Human-associated clones belonging to ST5, ST45, and ST239 MRSA clones were discovered in companion animals, which suggests anthropozoonotic transmission.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia",
volume = "8",
number = "1",
pages = "26",
doi = "10.3390/antibiotics8010026"
}

Ašanin, J., Mišić, D., Aksentijević, K., Tambur, Z., Rakonjac, B., Kovačević, I., Spergser, J.,& Loncarić, I.. (2019). Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel
MDPI, Basel., 8(1), 26.
https://doi.org/10.3390/antibiotics8010026

Ašanin J, Mišić D, Aksentijević K, Tambur Z, Rakonjac B, Kovačević I, Spergser J, Loncarić I. Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia. in Antibiotics-Basel. 2019;8(1):26.
doi:10.3390/antibiotics8010026 .

Ašanin, Jelena, Mišić, Dušan, Aksentijević, Ksenija, Tambur, Zoran, Rakonjac, Bojan, Kovačević, Ivana, Spergser, Joachim, Loncarić, Igor, "Genetic Profiling and Comparison of Human and Animal Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Serbia" in Antibiotics-Basel, 8, no. 1 (2019):26,
https://doi.org/10.3390/antibiotics8010026 . .

TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1775
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Loncarić, Igor
AU  - Kuebber-Heiss, Anna
AU  - Posautz, Annika
AU  - Ruppitsch, Werner
AU  - Lepuschitz, Sarah
AU  - Schauer, Bernhard
AU  - Fessler, Andrea T.
AU  - Krametter-Froetscher, Reinhild
AU  - Harrison, Ewan M.
AU  - Holmes, Mark A.
AU  - Kuenzel, Frank
AU  - Szostak, Michael
AU  - Hauschild, Tomasz
AU  - Desvars-Larrive, Amelie
AU  - Mišić, Dušan
AU  - Rosengarten, Renate
AU  - Walzer, Chris
AU  - Slickers, Peter
AU  - Monecke, Stefan
AU  - Ehricht, Ralf
AU  - Schwarz, Stefan
AU  - Spergser, Joachim
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1845
AB  - The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.
PB  - Elsevier, Amsterdam
T2  - Veterinary Microbiology
T1  - Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals
VL  - 230
SP  - 138
EP  - 144
DO  - 10.1016/j.vetmic.2019.02.014
ER  - 

@article{
author = "Loncarić, Igor and Kuebber-Heiss, Anna and Posautz, Annika and Ruppitsch, Werner and Lepuschitz, Sarah and Schauer, Bernhard and Fessler, Andrea T. and Krametter-Froetscher, Reinhild and Harrison, Ewan M. and Holmes, Mark A. and Kuenzel, Frank and Szostak, Michael and Hauschild, Tomasz and Desvars-Larrive, Amelie and Mišić, Dušan and Rosengarten, Renate and Walzer, Chris and Slickers, Peter and Monecke, Stefan and Ehricht, Ralf and Schwarz, Stefan and Spergser, Joachim",
year = "2019",
abstract = "The presence of the methicillin resistance gene mecC in coagulase-negative Staphylococcus spp. (CoNS) is scarce. The aim of this study was to characterize mecC-positive CoNS isolated from various wild and domestic animals. The presence of the mecC gene was screened in 4299 samples from wild animals and domestic animals. Fifteen coagulase-negative staphylococci, that displayed a cefoxitin-resistant phenotype, were tested mecC-positive by PCR. Antimicrobial susceptibility testing was performed for all isolates. The 15 isolates were genotyped by sequencing of the entire class E mec gene complex (blaZ-mecC-mecRI-mecI), the ccrA and ccrB recombinase genes and other determinants within the type XI SCCmec element. DNA microarray analysis was performed and five selected isolates were additionally whole genome sequenced and analyzed. S. stepanovicii (n = 3), S. caprae (n = 1), S. warneri (n = 1), S. xylosus (n = 1) and S. sciuri (n = 9) were detected. All but the S. sciuri isolates were found to be susceptible to all non-beta lactams. The entire class E mec gene complex was detected in all isolates but ccrA and ccrB genes were not identified in S. stepanovicii and S. xylosus. The genes erm(B) and fexA (n = 4, each) were the most predominant non-beta lactam resistance genes detected in the S. sciuri isolates. Even though the presence of the mecC gene among CoNS is a rare observation, this study further expands our knowledge by showing that the mecC gene, including its allotypes, are present in more staphylococcal species from different animal species than has been previously described.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Microbiology",
title = "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals",
volume = "230",
pages = "138-144",
doi = "10.1016/j.vetmic.2019.02.014"
}

Loncarić, I., Kuebber-Heiss, A., Posautz, A., Ruppitsch, W., Lepuschitz, S., Schauer, B., Fessler, A. T., Krametter-Froetscher, R., Harrison, E. M., Holmes, M. A., Kuenzel, F., Szostak, M., Hauschild, T., Desvars-Larrive, A., Mišić, D., Rosengarten, R., Walzer, C., Slickers, P., Monecke, S., Ehricht, R., Schwarz, S.,& Spergser, J.. (2019). Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology
Elsevier, Amsterdam., 230, 138-144.
https://doi.org/10.1016/j.vetmic.2019.02.014

Loncarić I, Kuebber-Heiss A, Posautz A, Ruppitsch W, Lepuschitz S, Schauer B, Fessler AT, Krametter-Froetscher R, Harrison EM, Holmes MA, Kuenzel F, Szostak M, Hauschild T, Desvars-Larrive A, Mišić D, Rosengarten R, Walzer C, Slickers P, Monecke S, Ehricht R, Schwarz S, Spergser J. Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals. in Veterinary Microbiology. 2019;230:138-144.
doi:10.1016/j.vetmic.2019.02.014 .

Loncarić, Igor, Kuebber-Heiss, Anna, Posautz, Annika, Ruppitsch, Werner, Lepuschitz, Sarah, Schauer, Bernhard, Fessler, Andrea T., Krametter-Froetscher, Reinhild, Harrison, Ewan M., Holmes, Mark A., Kuenzel, Frank, Szostak, Michael, Hauschild, Tomasz, Desvars-Larrive, Amelie, Mišić, Dušan, Rosengarten, Renate, Walzer, Chris, Slickers, Peter, Monecke, Stefan, Ehricht, Ralf, Schwarz, Stefan, Spergser, Joachim, "Characterization of mecC gene-carrying coagulase-negative Staphylococcus spp. isolated from various animals" in Veterinary Microbiology, 230 (2019):138-144,
https://doi.org/10.1016/j.vetmic.2019.02.014 . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Spergser, Joachim
AU  - Szostak, Michael
AU  - Loncarić, Igor
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1671
PB  - Amer Soc Microbiology, Washington
T2  - Antimicrobial Agents and Chemotherapy
T1  - OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia
VL  - 62
IS  - 7
SP  - e00219-18
DO  - 10.1128/AAC.00219-18
ER  - 

@article{
author = "Mišić, Dušan and Ašanin, Jelena and Spergser, Joachim and Szostak, Michael and Loncarić, Igor",
year = "2018",
publisher = "Amer Soc Microbiology, Washington",
journal = "Antimicrobial Agents and Chemotherapy",
title = "OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia",
volume = "62",
number = "7",
pages = "e00219-18",
doi = "10.1128/AAC.00219-18"
}

Mišić, D., Ašanin, J., Spergser, J., Szostak, M.,& Loncarić, I.. (2018). OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia. in Antimicrobial Agents and Chemotherapy
Amer Soc Microbiology, Washington., 62(7), e00219-18.
https://doi.org/10.1128/AAC.00219-18

Mišić D, Ašanin J, Spergser J, Szostak M, Loncarić I. OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia. in Antimicrobial Agents and Chemotherapy. 2018;62(7):e00219-18.
doi:10.1128/AAC.00219-18 .

Mišić, Dušan, Ašanin, Jelena, Spergser, Joachim, Szostak, Michael, Loncarić, Igor, "OXA-72-Mediated Carbapenem Resistance in Sequence Type 1 Multidrug (Colistin)-Resistant Acinetobacter baumannii Associated with Urinary Tract Infection in a Dog from Serbia" in Antimicrobial Agents and Chemotherapy, 62, no. 7 (2018):e00219-18,
https://doi.org/10.1128/AAC.00219-18 . .

TY  - JOUR
AU  - Marques, Catia
AU  - Gama, Luis Telo
AU  - Belas, Adriana
AU  - Bergstrom, Karin
AU  - Beurlet, Stephanie
AU  - Briend-Marchal, Alexandra
AU  - Broens, Els M.
AU  - Costa, Marta
AU  - Criel, Delphine
AU  - Damborg, Peter
AU  - van Dijk, Marloes A. M.
AU  - van Dongen, Astrid M.
AU  - Dorsch, Roswitha
AU  - Espada, Carmen Martin
AU  - Gerber, Bernhard
AU  - Kritsepi-Konstantinou, Maria
AU  - Loncarić, Igor
AU  - Mion, Domenico
AU  - Mišić, Dušan
AU  - Movilla, Rebeca
AU  - Overesch, Gudrun
AU  - Perreten, Vincent
AU  - Roura, Xavier
AU  - Steenbergen, Joachim
AU  - Timofte, Dorina
AU  - Wolf, Georg
AU  - Zanoni, Renato Giulio
AU  - Schmitt, Sarah
AU  - Guardabassi, Luca
AU  - Pomba, Constanca
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1389
AB  - Background: There is a growing concern regarding the increase of antimicrobial resistant bacteria in companion animals. Yet, there are no studies comparing the resistance levels of these organisms in European countries. The aim of this study was to investigate geographical and temporal trends of antimicrobial resistant bacteria causing urinary tract infection (UTI) in companion animals in Europe. The antimicrobial susceptibility of 22 256 bacteria isolated from dogs and cats with UTI was determined. Samples were collected between 2008 and 2013 from 16 laboratories of 14 European countries. The prevalence of antimicrobial resistance of the most common bacteria was determined for each country individually in the years 2012-2013 and temporal trends of bacteria resistance were established by logistic regression. Results: The aetiology of uropathogenic bacteria differed between dogs and cats. For all bacterial species, Southern countries generally presented higher levels of antimicrobial resistance compared to Northern countries. Multidrug-resistant Escherichia coli were found to be more prevalent in Southern countries. During the study period, the level of fluoroquinolone-resistant E. coli isolated in Belgium, Denmark, France and the Netherlands decreased significantly. A temporal increase in resistance to amoxicillin-clavulanate and gentamicin was observed among E. coli isolates from the Netherlands and Switzerland, respectively. Other country-specific temporal increases were observed for fluoroquinolone-resistant Proteus spp. isolated from companion animals from Belgium. Conclusions: This work brings new insights into the current status of antimicrobial resistance in bacteria isolated from companion animals with UTI in Europe and reinforces the need for strategies aiming to reduce resistance.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - European multicenter study on antimicrobial resistance in bacteria isolated from companion animal urinary tract infections
VL  - 12
SP  - 213
DO  - 10.1186/s12917-016-0840-3
ER  - 

@article{
author = "Marques, Catia and Gama, Luis Telo and Belas, Adriana and Bergstrom, Karin and Beurlet, Stephanie and Briend-Marchal, Alexandra and Broens, Els M. and Costa, Marta and Criel, Delphine and Damborg, Peter and van Dijk, Marloes A. M. and van Dongen, Astrid M. and Dorsch, Roswitha and Espada, Carmen Martin and Gerber, Bernhard and Kritsepi-Konstantinou, Maria and Loncarić, Igor and Mion, Domenico and Mišić, Dušan and Movilla, Rebeca and Overesch, Gudrun and Perreten, Vincent and Roura, Xavier and Steenbergen, Joachim and Timofte, Dorina and Wolf, Georg and Zanoni, Renato Giulio and Schmitt, Sarah and Guardabassi, Luca and Pomba, Constanca",
year = "2016",
abstract = "Background: There is a growing concern regarding the increase of antimicrobial resistant bacteria in companion animals. Yet, there are no studies comparing the resistance levels of these organisms in European countries. The aim of this study was to investigate geographical and temporal trends of antimicrobial resistant bacteria causing urinary tract infection (UTI) in companion animals in Europe. The antimicrobial susceptibility of 22 256 bacteria isolated from dogs and cats with UTI was determined. Samples were collected between 2008 and 2013 from 16 laboratories of 14 European countries. The prevalence of antimicrobial resistance of the most common bacteria was determined for each country individually in the years 2012-2013 and temporal trends of bacteria resistance were established by logistic regression. Results: The aetiology of uropathogenic bacteria differed between dogs and cats. For all bacterial species, Southern countries generally presented higher levels of antimicrobial resistance compared to Northern countries. Multidrug-resistant Escherichia coli were found to be more prevalent in Southern countries. During the study period, the level of fluoroquinolone-resistant E. coli isolated in Belgium, Denmark, France and the Netherlands decreased significantly. A temporal increase in resistance to amoxicillin-clavulanate and gentamicin was observed among E. coli isolates from the Netherlands and Switzerland, respectively. Other country-specific temporal increases were observed for fluoroquinolone-resistant Proteus spp. isolated from companion animals from Belgium. Conclusions: This work brings new insights into the current status of antimicrobial resistance in bacteria isolated from companion animals with UTI in Europe and reinforces the need for strategies aiming to reduce resistance.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "European multicenter study on antimicrobial resistance in bacteria isolated from companion animal urinary tract infections",
volume = "12",
pages = "213",
doi = "10.1186/s12917-016-0840-3"
}

Marques, C., Gama, L. T., Belas, A., Bergstrom, K., Beurlet, S., Briend-Marchal, A., Broens, E. M., Costa, M., Criel, D., Damborg, P., van Dijk, M. A. M., van Dongen, A. M., Dorsch, R., Espada, C. M., Gerber, B., Kritsepi-Konstantinou, M., Loncarić, I., Mion, D., Mišić, D., Movilla, R., Overesch, G., Perreten, V., Roura, X., Steenbergen, J., Timofte, D., Wolf, G., Zanoni, R. G., Schmitt, S., Guardabassi, L.,& Pomba, C.. (2016). European multicenter study on antimicrobial resistance in bacteria isolated from companion animal urinary tract infections. in BMC Veterinary Research
BMC, London., 12, 213.
https://doi.org/10.1186/s12917-016-0840-3

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Marques, Catia, Gama, Luis Telo, Belas, Adriana, Bergstrom, Karin, Beurlet, Stephanie, Briend-Marchal, Alexandra, Broens, Els M., Costa, Marta, Criel, Delphine, Damborg, Peter, van Dijk, Marloes A. M., van Dongen, Astrid M., Dorsch, Roswitha, Espada, Carmen Martin, Gerber, Bernhard, Kritsepi-Konstantinou, Maria, Loncarić, Igor, Mion, Domenico, Mišić, Dušan, Movilla, Rebeca, Overesch, Gudrun, Perreten, Vincent, Roura, Xavier, Steenbergen, Joachim, Timofte, Dorina, Wolf, Georg, Zanoni, Renato Giulio, Schmitt, Sarah, Guardabassi, Luca, Pomba, Constanca, "European multicenter study on antimicrobial resistance in bacteria isolated from companion animal urinary tract infections" in BMC Veterinary Research, 12 (2016):213,
https://doi.org/10.1186/s12917-016-0840-3 . .