TY  - JOUR
AU  - Dacić, M.
AU  - Resanović, Radmila
AU  - Rašić, Zoran
AU  - Valčić, Miroslav
AU  - Milovanović, A.
AU  - Velhner, Maja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1623
AB  - The infectious bursal disease virus (IBDv) is widespread in poultry flocks all around the world. Various biotypes have emerged and because of that, adequate management practices and vaccination of chicks are of paramount importance for the protection against field strains. One day old Lohmann Brown chicks were vaccinated with intermediate vaccines and the recombinant VAXXITEK HVT-IBDv vaccine formulation, and challenged at 48 days of life with the very virulent IBDv (vvIBDv) strain CH/99. The best protection (100%) was achieved with the recombinant vaccine administered by the subcutaneous or intramuscular route at a day old, while intermediate and intermediate plus vaccines protected 80% of birds from clinical symptoms. The highest bursa body ratio (5.33, 3.50 and 4.12) was accomplished in non-vaccinated and non-challenged birds and birds vaccinated with the VAXXITEK HVT-IBDv vaccine. The recombinant VAXXITEK HVT-IBDv vaccine has provided protection for commercial chicks against challenge with the vvIBDv strain in this experiment. Under field conditions, additional vaccination is possibly needed with supplementary application of live attenuated vaccines. However, the recombinant vector vaccines are providing significant aid against clinical signs and immunosupression caused by the vvIBDv.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study
VL  - 69
IS  - 1
SP  - 823
EP  - 830
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1623
ER  - 

@article{
author = "Dacić, M. and Resanović, Radmila and Rašić, Zoran and Valčić, Miroslav and Milovanović, A. and Velhner, Maja",
year = "2018",
abstract = "The infectious bursal disease virus (IBDv) is widespread in poultry flocks all around the world. Various biotypes have emerged and because of that, adequate management practices and vaccination of chicks are of paramount importance for the protection against field strains. One day old Lohmann Brown chicks were vaccinated with intermediate vaccines and the recombinant VAXXITEK HVT-IBDv vaccine formulation, and challenged at 48 days of life with the very virulent IBDv (vvIBDv) strain CH/99. The best protection (100%) was achieved with the recombinant vaccine administered by the subcutaneous or intramuscular route at a day old, while intermediate and intermediate plus vaccines protected 80% of birds from clinical symptoms. The highest bursa body ratio (5.33, 3.50 and 4.12) was accomplished in non-vaccinated and non-challenged birds and birds vaccinated with the VAXXITEK HVT-IBDv vaccine. The recombinant VAXXITEK HVT-IBDv vaccine has provided protection for commercial chicks against challenge with the vvIBDv strain in this experiment. Under field conditions, additional vaccination is possibly needed with supplementary application of live attenuated vaccines. However, the recombinant vector vaccines are providing significant aid against clinical signs and immunosupression caused by the vvIBDv.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study",
volume = "69",
number = "1",
pages = "823-830",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1623"
}

Dacić, M., Resanović, R., Rašić, Z., Valčić, M., Milovanović, A.,& Velhner, M.. (2018). Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(1), 823-830.
https://hdl.handle.net/21.15107/rcub_veterinar_1623

Dacić M, Resanović R, Rašić Z, Valčić M, Milovanović A, Velhner M. Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study. in Journal of The Hellenic Veterinary Medical Society. 2018;69(1):823-830.
https://hdl.handle.net/21.15107/rcub_veterinar_1623 .

Dacić, M., Resanović, Radmila, Rašić, Zoran, Valčić, Miroslav, Milovanović, A., Velhner, Maja, "Efficacy of recombinant VAXXITEK HVT-IBDv vaccine against very virulent Infectious bursal disease virus (vvIBDv) challenge in layer chicks: A pilot study" in Journal of The Hellenic Veterinary Medical Society, 69, no. 1 (2018):823-830,
https://hdl.handle.net/21.15107/rcub_veterinar_1623 .

TY  - JOUR
AU  - Apić, Jelena
AU  - Stančić, Ivan
AU  - Vakanjac, Slobodanka
AU  - Radović, I.
AU  - Milovanović, A.
AU  - Barna, Tomislav
AU  - Maletić, Milan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1333
AB  - The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.
PB  - Colegio Brasileiro de Reproducao Animal
T2  - Animal Reproduction
T1  - Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days
VL  - 13
IS  - 1
SP  - 36
EP  - 41
DO  - 10.4322/1984-3143-AR792
ER  - 

@article{
author = "Apić, Jelena and Stančić, Ivan and Vakanjac, Slobodanka and Radović, I. and Milovanović, A. and Barna, Tomislav and Maletić, Milan",
year = "2016",
abstract = "The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.",
publisher = "Colegio Brasileiro de Reproducao Animal",
journal = "Animal Reproduction",
title = "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days",
volume = "13",
number = "1",
pages = "36-41",
doi = "10.4322/1984-3143-AR792"
}

Apić, J., Stančić, I., Vakanjac, S., Radović, I., Milovanović, A., Barna, T.,& Maletić, M.. (2016). Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction
Colegio Brasileiro de Reproducao Animal., 13(1), 36-41.
https://doi.org/10.4322/1984-3143-AR792

Apić J, Stančić I, Vakanjac S, Radović I, Milovanović A, Barna T, Maletić M. Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction. 2016;13(1):36-41.
doi:10.4322/1984-3143-AR792 .

Apić, Jelena, Stančić, Ivan, Vakanjac, Slobodanka, Radović, I., Milovanović, A., Barna, Tomislav, Maletić, Milan, "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days" in Animal Reproduction, 13, no. 1 (2016):36-41,
https://doi.org/10.4322/1984-3143-AR792 . .

TY  - CONF
AU  - Milovanović, A.
AU  - Bana, T.
AU  - Lazarević, Miodrag
AU  - Apić, Jelena
AU  - Milanov, Dubravka
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1303
AB  - Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.
PB  - Wiley-Blackwell, Hoboken
C3  - Reproduction in Domestic Animals
T1  - Pararectal treatment of boars with primary semen contamination and astenospermia
VL  - 50
SP  - 67
EP  - 67
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1303
ER  - 

@conference{
author = "Milovanović, A. and Bana, T. and Lazarević, Miodrag and Apić, Jelena and Milanov, Dubravka",
year = "2015",
abstract = "Importing boars of high genetic value is an important issue in Serbia.
Therefore, frequent replacement of boars is not a primary approach
and there is a need for their prolonged exploitation. Ejaculates from
6 boars were selected for bacterial control and for treatment based
on lower CASA (Computer Assisted Semen Analyses) results, flow
cytometry and cytological sperm quality parameters in extended
semen. According to the sensitivity of bacterial isolates from fresh
semen, boars were treated with Enroxil  10% (1 ml/40 kg BW;
Krka, Slovenia), during 8 consecutive days. An antibiotic was
injected between the tail and rectum (pararectal therapy). On the 8th
day, boars received 8 ml of Baytril  10% (Bayer, Germany). On the
first and last day of treatment boars received 20 ml of AD3E
vitamins with selenium i/m and C vitamin (20 ml, 100 mg/ml).
Semen quality was controlled 45 days following treatment. Total and
progressive sperm motility increased from 39.66% and 12.87% to
83.98% and 61.98%, respectively (p < 0.001). The percentage of live
spermatozoa with intact acrosomes also improved (48.3–63.4%,
PNA-FITC, n.s.). The percentage of agglutinated cells decreased
from 66.00 to 6.67% and the average bacterial contamination in
fresh semen decreased from 18.450 to 1.233 CFU/ml. It is evident
that boars with primary semen contamination are able to renew
sperm production after prolonged antibiotic and supportive treatment. This could be a strategy to decrease the culling rate in elite
stud boars.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Reproduction in Domestic Animals",
title = "Pararectal treatment of boars with primary semen contamination and astenospermia",
volume = "50",
pages = "67-67",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1303"
}

Milovanović, A., Bana, T., Lazarević, M., Apić, J.,& Milanov, D.. (2015). Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals
Wiley-Blackwell, Hoboken., 50, 67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303

Milovanović A, Bana T, Lazarević M, Apić J, Milanov D. Pararectal treatment of boars with primary semen contamination and astenospermia. in Reproduction in Domestic Animals. 2015;50:67-67.
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

Milovanović, A., Bana, T., Lazarević, Miodrag, Apić, Jelena, Milanov, Dubravka, "Pararectal treatment of boars with primary semen contamination and astenospermia" in Reproduction in Domestic Animals, 50 (2015):67-67,
https://hdl.handle.net/21.15107/rcub_veterinar_1303 .

TY  - CONF
AU  - Milovanović, A.
AU  - Barna, Tomislav
AU  - Jovičin, Milovan
AU  - Lazarević, Miodrag
AU  - Nakao, T.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/714
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Reproduction in Domestic Animals
T1  - Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows
VL  - 45
SP  - 99
EP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_714
ER  - 

@conference{
author = "Milovanović, A. and Barna, Tomislav and Jovičin, Milovan and Lazarević, Miodrag and Nakao, T.",
year = "2010",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Reproduction in Domestic Animals",
title = "Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows",
volume = "45",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_714"
}

Milovanović, A., Barna, T., Jovičin, M., Lazarević, M.,& Nakao, T.. (2010). Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows. in Reproduction in Domestic Animals
Wiley-Blackwell Publishing, Inc, Malden., 45, 99-99.
https://hdl.handle.net/21.15107/rcub_veterinar_714

Milovanović A, Barna T, Jovičin M, Lazarević M, Nakao T. Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows. in Reproduction in Domestic Animals. 2010;45:99-99.
https://hdl.handle.net/21.15107/rcub_veterinar_714 .

Milovanović, A., Barna, Tomislav, Jovičin, Milovan, Lazarević, Miodrag, Nakao, T., "Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows" in Reproduction in Domestic Animals, 45 (2010):99-99,
https://hdl.handle.net/21.15107/rcub_veterinar_714 .