TY  - JOUR
AU  - Đurđević, S.
AU  - Jezdimirović, Milanka
AU  - Đurović, A.
AU  - Dedić, Gordana
AU  - Aleksić, Nevenka
AU  - Branković, Nataša
AU  - Stojiljković, S.
AU  - Stojšić, D.
AU  - Blagojević, Z.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/569
AB  - The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints.
AB  - Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig
T1  - Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje
VL  - 59
IS  - 2-3
SP  - 157
EP  - 165
DO  - 10.2298/AVB0903157D
ER  - 

@article{
author = "Đurđević, S. and Jezdimirović, Milanka and Đurović, A. and Dedić, Gordana and Aleksić, Nevenka and Branković, Nataša and Stojiljković, S. and Stojšić, D. and Blagojević, Z.",
year = "2009",
abstract = "The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints., Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig, Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje",
volume = "59",
number = "2-3",
pages = "157-165",
doi = "10.2298/AVB0903157D"
}

Đurđević, S., Jezdimirović, M., Đurović, A., Dedić, G., Aleksić, N., Branković, N., Stojiljković, S., Stojšić, D.,& Blagojević, Z.. (2009). The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 157-165.
https://doi.org/10.2298/AVB0903157D

Đurđević S, Jezdimirović M, Đurović A, Dedić G, Aleksić N, Branković N, Stojiljković S, Stojšić D, Blagojević Z. The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd. 2009;59(2-3):157-165.
doi:10.2298/AVB0903157D .

Đurđević, S., Jezdimirović, Milanka, Đurović, A., Dedić, Gordana, Aleksić, Nevenka, Branković, Nataša, Stojiljković, S., Stojšić, D., Blagojević, Z., "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig" in Acta Veterinaria-Beograd, 59, no. 2-3 (2009):157-165,
https://doi.org/10.2298/AVB0903157D . .