TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1488
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
SP  - 8762649
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
pages = "8762649",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 8762649.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;:8762649.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science (2017):8762649,
https://doi.org/10.1155/2017/8762649 . .

TY  - JOUR
AU  - Fanovich, M. A.
AU  - Ivanović, J.
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Jaeger, Philip
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1369
AB  - Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2
VL  - 58
SP  - 204
EP  - 212
DO  - 10.1016/j.msec.2015.08.024
ER  - 

@article{
author = "Fanovich, M. A. and Ivanović, J. and Žižović, Irena and Mišić, Dušan and Jaeger, Philip",
year = "2016",
abstract = "Investigation of an integrated supercritical fluid extraction and supercritical solvent impregnation process for fabrication of microporous polycaprolactone-hydroxyapatite (PCL-HA) scaffolds with antibacterial activity is presented. The HA content and particle size as well as the operating conditions of the integrated process is optimized regarding the amount of impregnated antibacterial agent (Usnea lethariiformis extract) in the PCL-HA matrix, scaffold morphology and antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) strains. High pressure differential scanning calorimetry (HP-DSC) assay reveals that an increasing amount of HA results in decreasing melting temperature as well as crystallinity at an operating pressure of 17 MPa. The PCL-HA composites with micrometric sizes of the HA particles are convenient for being processed by the integrated process due to the simple preparation, a good interaction between the PCL matrix and filler and the advantageous impact on sorption. The scaffold obtained from PCL-HA with 20% of the HA shows the highest impregnation yield at 17 MPa and 35 degrees C (5.9%) and subsequently also the best bactericidal effect on the tested MRSA strains at an initial bacterial inoculum of 2 x 10(-4) CFU/mL",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2",
volume = "58",
pages = "204-212",
doi = "10.1016/j.msec.2015.08.024"
}

Fanovich, M. A., Ivanović, J., Žižović, I., Mišić, D.,& Jaeger, P.. (2016). Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 58, 204-212.
https://doi.org/10.1016/j.msec.2015.08.024

Fanovich MA, Ivanović J, Žižović I, Mišić D, Jaeger P. Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2. in Materials Science & Engineering C-Materials for Biological Applications. 2016;58:204-212.
doi:10.1016/j.msec.2015.08.024 .

Fanovich, M. A., Ivanović, J., Žižović, Irena, Mišić, Dušan, Jaeger, Philip, "Functionalization of polycaprolactone/hydroxyapatite scaffolds with Usnea lethariiformis extract by using supercritical CO2" in Materials Science & Engineering C-Materials for Biological Applications, 58 (2016):204-212,
https://doi.org/10.1016/j.msec.2015.08.024 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Aksentijević, Ksenija
AU  - Stojanović, Dusica B.
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1405
AB  - Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Application of cellulose acetate for controlled release of thymol
VL  - 147
SP  - 344
EP  - 353
DO  - 10.1016/j.carbpol.2016.03.093
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Aksentijević, Ksenija and Stojanović, Dusica B. and Ivanović, Jasna and Žižović, Irena",
year = "2016",
abstract = "Cellulose acetate (CA) was investigated as a carrier towards development of material with controlled release of thymol as a natural substance with strong antibacterial properties using high pressure techniques. Effect of thymol content on CA was confirmed by SEM, FTIR and DSC methods. Kinetic of thymol release from CA was tested using simulated gastric and intestinal fluids (hydrochloric acid and phosphate buffer saline). Results were correlated with Korsmeyer-Peppas and Weibull model. Depending on the thymol content and chemical nature of the release medium, the time of thymol release varied from one to three days indicating CA as a promising carrier of thymol with potential uses from medicine to agriculture. The impregnated CA showed antibacterial activity against 23 tested bacterial strains including methicillin-resistant Staphylococcus aureus (MRSA) which is particularly important bearing in mind that this strain causes fatal infections in humans and animals.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Application of cellulose acetate for controlled release of thymol",
volume = "147",
pages = "344-353",
doi = "10.1016/j.carbpol.2016.03.093"
}

Milovanović, S., Marković, D., Aksentijević, K., Stojanović, D. B., Ivanović, J.,& Žižović, I.. (2016). Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 147, 344-353.
https://doi.org/10.1016/j.carbpol.2016.03.093

Milovanović S, Marković D, Aksentijević K, Stojanović DB, Ivanović J, Žižović I. Application of cellulose acetate for controlled release of thymol. in Carbohydrate Polymers. 2016;147:344-353.
doi:10.1016/j.carbpol.2016.03.093 .

Milovanović, Stoja, Marković, Darka, Aksentijević, Ksenija, Stojanović, Dusica B., Ivanović, Jasna, Žižović, Irena, "Application of cellulose acetate for controlled release of thymol" in Carbohydrate Polymers, 147 (2016):344-353,
https://doi.org/10.1016/j.carbpol.2016.03.093 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Meyer, Florian
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1015
AB  - Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.
PB  - Elsevier, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent
VL  - 76
SP  - 1
EP  - 9
DO  - 10.1016/j.supflu.2013.01.005
ER  - 

@article{
author = "Ivanović, Jasna and Meyer, Florian and Mišić, Dušan and Ašanin, Jelena and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "Multiplication and spreading of multiresistant pathogenic bacteria that cause fatal infections in humans and animals have boosted research on implementation of newer technologies for producing natural substances with strong antibacterial activities. In this study, SFE from lichen Usnea barbata was investigated for different pre-treatment methods with respect to extracts yield, usnic acid recovery and antibacterial activity against more than thirty methicillin resistant staphylococci strains. Extraction of U. barbata with subcritical carbon dioxide was studied as well. Extraction yield, usnic acid content and antibacterial activity of the extracts were strongly affected by the pre-treatment method. Intensive pre-treatment methods such as flaking and cutting + grinding caused a considerable comminution of the lichen thallus and enabled high extraction yields. On the other hand, mild pre-treatment of the lichen such as impact + shearing enabled isolation of extracts with lower MIC values. Experimental results indicated that extraction with liquid carbon dioxide at 30 MPa and 25 degrees C was the most favorable condition at which a satisfactory high extraction yield was obtained (2.08%). Extract isolated with liquid carbon dioxide had a high usnic acid content in the extract (632 g/kg) and a very strong antibacterial activity (average MIC of 19.11 mu g/mL). Antibacterial activity of all the extracts against tested strains was comparable to the pure usnic acid or even stronger. This study indicated a potential use of the supercritical extracts of U. barbata in pharmaceutical formulations for treatment of infections caused by staphylococci.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent",
volume = "76",
pages = "1-9",
doi = "10.1016/j.supflu.2013.01.005"
}

Ivanović, J., Meyer, F., Mišić, D., Ašanin, J., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids
Elsevier, Amsterdam., 76, 1-9.
https://doi.org/10.1016/j.supflu.2013.01.005

Ivanović J, Meyer F, Mišić D, Ašanin J, Jaeger P, Žižović I, Eggers R. Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent. in Journal of Supercritical Fluids. 2013;76:1-9.
doi:10.1016/j.supflu.2013.01.005 .

Ivanović, Jasna, Meyer, Florian, Mišić, Dušan, Ašanin, Jelena, Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Influence of different pre-treatment methods on isolation of extracts with strong antibacterial activity from lichen Usnea barbata using carbon dioxide as a solvent" in Journal of Supercritical Fluids, 76 (2013):1-9,
https://doi.org/10.1016/j.supflu.2013.01.005 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Dimitrijević-Branković, Suzana
AU  - Mišić, Dušan
AU  - Ristić, Mihailo
AU  - Žižović, Irena
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1019
AB  - For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds
VL  - 5
IS  - 1
SP  - 416
EP  - 423
DO  - 10.1016/j.jff.2012.11.014
ER  - 

@article{
author = "Ivanović, Jasna and Dimitrijević-Branković, Suzana and Mišić, Dušan and Ristić, Mihailo and Žižović, Irena",
year = "2013",
abstract = "For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds",
volume = "5",
number = "1",
pages = "416-423",
doi = "10.1016/j.jff.2012.11.014"
}

Ivanović, J., Dimitrijević-Branković, S., Mišić, D., Ristić, M.,& Žižović, I.. (2013). Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods
Elsevier, Amsterdam., 5(1), 416-423.
https://doi.org/10.1016/j.jff.2012.11.014

Ivanović J, Dimitrijević-Branković S, Mišić D, Ristić M, Žižović I. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods. 2013;5(1):416-423.
doi:10.1016/j.jff.2012.11.014 .

Ivanović, Jasna, Dimitrijević-Branković, Suzana, Mišić, Dušan, Ristić, Mihailo, Žižović, Irena, "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds" in Journal of Functional Foods, 5, no. 1 (2013):416-423,
https://doi.org/10.1016/j.jff.2012.11.014 . .

TY  - JOUR
AU  - Fanovich, M. A.
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Alvarez, M. V.
AU  - Jaeger, Philip
AU  - Žižović, Irena
AU  - Eggers, Rudolf
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1020
AB  - The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process
VL  - 78
SP  - 42
EP  - 53
DO  - 10.1016/j.supflu.2013.03.017
ER  - 

@article{
author = "Fanovich, M. A. and Ivanović, Jasna and Mišić, Dušan and Alvarez, M. V. and Jaeger, Philip and Žižović, Irena and Eggers, Rudolf",
year = "2013",
abstract = "The present study is aimed to develop a process for production of functionalized scaffolds impregnated with natural compounds extracted from Patagonian Usnea lichen. A setup for an integrated supercritical CO2 extraction of natural compounds with posterior impregnation on solid matrices (polycaprolactone, PCL) is developed and presented here. In order to establish optimized operating conditions, supercritical extraction of Usnea as well as sorption kinetics and resulting material properties have been studied separately first. Usnea extracts isolated by supercritical carbon dioxide at 30 MPa and 40 degrees C have shown strong antibacterial activity with values of the minimum inhibitory concentration (MIC) ranging from less than 1.25 mu g/mL to 320 mu g/mL against Listerin innocua and Methicillin-resistant Staphylococcus (MRS) strains. Useful scaffolds of PCL for tissue engineering containing a porous structure with pore diameters between 150 and 340 pm can be obtained when PCL is exposed to carbon dioxide at 35 degrees C and 15 MPa. The degree of crystallinity of functionalized PCL was shown to be influenced by the incorporated antibacterial agent. The presented results showed that the impregnated PCL samples are promising bactericidal compounds against L innocua. Screening of antibacterial activity of functionalized PCL against a Methicillin-resistant Staphylococcus aureus (MRSA) strain showed a higher activity when a low bacterial inoculum level (2 x 10(4) Colony-forming Units/mL [CFU/mL]) was assayed.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process",
volume = "78",
pages = "42-53",
doi = "10.1016/j.supflu.2013.03.017"
}

Fanovich, M. A., Ivanović, J., Mišić, D., Alvarez, M. V., Jaeger, P., Žižović, I.,& Eggers, R.. (2013). Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 78, 42-53.
https://doi.org/10.1016/j.supflu.2013.03.017

Fanovich MA, Ivanović J, Mišić D, Alvarez MV, Jaeger P, Žižović I, Eggers R. Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process. in Journal of Supercritical Fluids. 2013;78:42-53.
doi:10.1016/j.supflu.2013.03.017 .

Fanovich, M. A., Ivanović, Jasna, Mišić, Dušan, Alvarez, M. V., Jaeger, Philip, Žižović, Irena, Eggers, Rudolf, "Development of polycaprolactone scaffold with antibacterial activity by an integrated supercritical extraction and impregnation process" in Journal of Supercritical Fluids, 78 (2013):42-53,
https://doi.org/10.1016/j.supflu.2013.03.017 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukic-Marković, Jelena
AU  - Petrović, Slobodan D.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/905
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
VL  - 72
SP  - 7
EP  - 14
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Žižović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukic-Marković, Jelena and Petrović, Slobodan D.",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
volume = "72",
pages = "7-14",
doi = "10.1016/j.supflu.2012.07.018"
}

Žižović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukic-Marković, J.,& Petrović, S. D.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Žižović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukic-Marković J, Petrović SD. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Žižović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukic-Marković, Jelena, Petrović, Slobodan D., "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Ristić, Mihailo
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/873
AB  - Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Control
T1  - In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates
VL  - 25
IS  - 1
SP  - 110
EP  - 116
DO  - 10.1016/j.foodcont.2011.10.019
ER  - 

@article{
author = "Ivanović, Jasna and Mišić, Dušan and Žižović, Irena and Ristić, Mihailo",
year = "2012",
abstract = "Antibacterial activity of thyme, rosemary and sage isolates obtained by supercritical fluid extraction and hydrodistillation was investigated on Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and Escherichia coli strains. Bacillus species were the most susceptible to all tested isolates. The thyme isolates showed the strongest antibacterial activity against tested bacteria with MIC values of 40-640 mu g/ml, followed by rosemary (MIC = 320-1280 mu g/ml) and sage (MIC = 160-2560 mu g/ml) isolates. Therefore, the antibacterial activity of the most abundant components found in the thyme isolates, thymol, p-cymene and their mixture was investigated as well. The thyme isolates, especially supercritical extract, showed stronger antibacterial activity against Bacillus strains compared to the single components and their mixture, which indicated synergetic effect of the other components. Results of this study indicated thyme as a valuable source of natural antibacterial agents and supercritical fluid extraction as an efficient isolation method.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Control",
title = "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates",
volume = "25",
number = "1",
pages = "110-116",
doi = "10.1016/j.foodcont.2011.10.019"
}

Ivanović, J., Mišić, D., Žižović, I.,& Ristić, M.. (2012). In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control
Elsevier Sci Ltd, Oxford., 25(1), 110-116.
https://doi.org/10.1016/j.foodcont.2011.10.019

Ivanović J, Mišić D, Žižović I, Ristić M. In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates. in Food Control. 2012;25(1):110-116.
doi:10.1016/j.foodcont.2011.10.019 .

Ivanović, Jasna, Mišić, Dušan, Žižović, Irena, Ristić, Mihailo, "In vitro control of multiplication of some food-associated bacteria by thyme, rosemary and sage isolates" in Food Control, 25, no. 1 (2012):110-116,
https://doi.org/10.1016/j.foodcont.2011.10.019 . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Ivanović, Jasna
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/721
AB  - Antibacterial effects of plant extracts were examined using mixtures of extracts obtained using different technological processes: usnea extract was obtained using the process of supercritical extraction (NKE), angelica extract was obtained through supercritical extraction processes (NKE) and ultrasound extraction using ethanol (UZ), and thyme extract was obtained using the process of hydrodistillation (HD). Mixtures of the listed extracts were examined in various ratios: U (NKE) and T (HD) in a ratio of 1:1, U (NKE) and T (HD) in a ratio of 7:3, U (NKE), T (HD) and A (NKE) in a ratio of 2:2:1, and U (NKE), T (HD) and A (UZ) in a ratio of 2:2:1. The investigations covered 15 strains of bacteria of the genus Staphylococcus, Streptococcus and Enterococus, including the strains MRSA, VRE as well as reference strains of S. pyogenes ATCC 19615, S. agalactiae ATCC 27959 and S. aureus ATCC 11632. The antibacterial action of mixes of plant extracts was examined using the microdilution method in bouillon, and the examined mix concentrations were from 1.25 g/mL to 1280 g/mL. The strongest antibacterial effect was exhibited by mixes of usnea (NKE) and thyme (HD) in ratios of 1:1 and 7:3 with obtained MIC values from 5 g/mL to 160 g/mL, but the MIC value of the listed mixtures for the biggest number of strains amounted to 40 g/mL. A somewhat weaker effect was exhibited by the other examined extract mixtures with obtained MIC values of 10 g/mL to 320 g/mL. Based on the obtained MIC values and the results of previous investigations, it can be concluded that the examined mixtures of plant extracts exhibited a very strong antibacterial effect on the examined bacteria strains. .
AB  - U ispitivanjima antibakterijskog delovanja biljnih ekstrakata korišćene su mešavine ekstrakata dobijenih primenom različitih tehnoloških procesa i to: ekstrakt usnee dobijen procesom natkritične ekstrakcije (NKE), ekstrakti anđelike dobijeni procesima natkritične ekstrakcije (NKE) i ultrazvučne ekstrakcije etanolom (UZ) i ekstrakt timijana dobijen procesom hidrodestilacije (HD). Ispitivane su mešavine navedenih ekstrakata u različitim odnosima: U(NKE) i T(HD) u odnosu 1:1, U(NKE) i T(HD) u odnosu 7:3, U(NKE), T(HD) i A(NKE) u odnosu 2:2:1 i U(NKE), T(HD) i A(UZ) u odnosu 2:2:1. Ispitivanjem je bilo obuhvaćeno 15 sojeva bakterija iz rodova Staphylococcus, Streptococcus i Enterococus, uključujući sojeve MRSA, sojeve VRE kao i referentne sojeve S. pyogenes ATCC 19615, S. agalactiae ATCC 27959 i S. aureus ATCC 11632. Antibakterijsko delovanje mešavina biljnih ekstrakata ispitivano je primenom mikrodilucione metode u bujonu, a ispitivane su koncentracije mešavina od 1,25 g/mL do 1280 g/mL. Najjače antibakterijsko delovanje pokazale su mešavine usnee (NKE) i timijana (HD) u odnosu 1:1 i 7:3 sa dobijenim vrednostima MIC od 5 g/mL do 160 g/mL, ali je vrednost MIC navedenih mešavina za najveći broj sojeva iznosila 40 g/mL. Nešto slabije delovanje pokazale su ostale ispitivane mešavine ekstrakata sa dobijenim vrednostima MIC od 10 g/mL do 320 g/mL. S obzirom na dobijene vrednosti MIC kao i na osnovu rezultata ranijih istraživanja, može se zaključiti da su sve ispitivane mešavine biljnih ekstrakata pokazale veoma jako antibakterijsko delovanje na ispitivane sojeve bakterija. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine
T1  - Antibakterijsko delovanje mešavina ekstrakata usnee, timijana i anđelike dobijenih različitim tehnološkim procesima protiv nekih vrsta bakterija značajnih za veterinarsku medicinu
VL  - 64
IS  - 1-2
SP  - 65
EP  - 74
DO  - 10.2298/VETGL1002065M
ER  - 

@article{
author = "Mišić, Dušan and Žižović, Irena and Ivanović, Jasna",
year = "2010",
abstract = "Antibacterial effects of plant extracts were examined using mixtures of extracts obtained using different technological processes: usnea extract was obtained using the process of supercritical extraction (NKE), angelica extract was obtained through supercritical extraction processes (NKE) and ultrasound extraction using ethanol (UZ), and thyme extract was obtained using the process of hydrodistillation (HD). Mixtures of the listed extracts were examined in various ratios: U (NKE) and T (HD) in a ratio of 1:1, U (NKE) and T (HD) in a ratio of 7:3, U (NKE), T (HD) and A (NKE) in a ratio of 2:2:1, and U (NKE), T (HD) and A (UZ) in a ratio of 2:2:1. The investigations covered 15 strains of bacteria of the genus Staphylococcus, Streptococcus and Enterococus, including the strains MRSA, VRE as well as reference strains of S. pyogenes ATCC 19615, S. agalactiae ATCC 27959 and S. aureus ATCC 11632. The antibacterial action of mixes of plant extracts was examined using the microdilution method in bouillon, and the examined mix concentrations were from 1.25 g/mL to 1280 g/mL. The strongest antibacterial effect was exhibited by mixes of usnea (NKE) and thyme (HD) in ratios of 1:1 and 7:3 with obtained MIC values from 5 g/mL to 160 g/mL, but the MIC value of the listed mixtures for the biggest number of strains amounted to 40 g/mL. A somewhat weaker effect was exhibited by the other examined extract mixtures with obtained MIC values of 10 g/mL to 320 g/mL. Based on the obtained MIC values and the results of previous investigations, it can be concluded that the examined mixtures of plant extracts exhibited a very strong antibacterial effect on the examined bacteria strains. ., U ispitivanjima antibakterijskog delovanja biljnih ekstrakata korišćene su mešavine ekstrakata dobijenih primenom različitih tehnoloških procesa i to: ekstrakt usnee dobijen procesom natkritične ekstrakcije (NKE), ekstrakti anđelike dobijeni procesima natkritične ekstrakcije (NKE) i ultrazvučne ekstrakcije etanolom (UZ) i ekstrakt timijana dobijen procesom hidrodestilacije (HD). Ispitivane su mešavine navedenih ekstrakata u različitim odnosima: U(NKE) i T(HD) u odnosu 1:1, U(NKE) i T(HD) u odnosu 7:3, U(NKE), T(HD) i A(NKE) u odnosu 2:2:1 i U(NKE), T(HD) i A(UZ) u odnosu 2:2:1. Ispitivanjem je bilo obuhvaćeno 15 sojeva bakterija iz rodova Staphylococcus, Streptococcus i Enterococus, uključujući sojeve MRSA, sojeve VRE kao i referentne sojeve S. pyogenes ATCC 19615, S. agalactiae ATCC 27959 i S. aureus ATCC 11632. Antibakterijsko delovanje mešavina biljnih ekstrakata ispitivano je primenom mikrodilucione metode u bujonu, a ispitivane su koncentracije mešavina od 1,25 g/mL do 1280 g/mL. Najjače antibakterijsko delovanje pokazale su mešavine usnee (NKE) i timijana (HD) u odnosu 1:1 i 7:3 sa dobijenim vrednostima MIC od 5 g/mL do 160 g/mL, ali je vrednost MIC navedenih mešavina za najveći broj sojeva iznosila 40 g/mL. Nešto slabije delovanje pokazale su ostale ispitivane mešavine ekstrakata sa dobijenim vrednostima MIC od 10 g/mL do 320 g/mL. S obzirom na dobijene vrednosti MIC kao i na osnovu rezultata ranijih istraživanja, može se zaključiti da su sve ispitivane mešavine biljnih ekstrakata pokazale veoma jako antibakterijsko delovanje na ispitivane sojeve bakterija. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine, Antibakterijsko delovanje mešavina ekstrakata usnee, timijana i anđelike dobijenih različitim tehnološkim procesima protiv nekih vrsta bakterija značajnih za veterinarsku medicinu",
volume = "64",
number = "1-2",
pages = "65-74",
doi = "10.2298/VETGL1002065M"
}

Mišić, D., Žižović, I.,& Ivanović, J.. (2010). Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1-2), 65-74.
https://doi.org/10.2298/VETGL1002065M

Mišić D, Žižović I, Ivanović J. Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine. in Veterinarski Glasnik. 2010;64(1-2):65-74.
doi:10.2298/VETGL1002065M .

Mišić, Dušan, Žižović, Irena, Ivanović, Jasna, "Antibacterial effects of mixtures of extracts of usnea, thyme and angelica obtained using different technological processes against certain types of bacteria of importance in veterinary medicine" in Veterinarski Glasnik, 64, no. 1-2 (2010):65-74,
https://doi.org/10.2298/VETGL1002065M . .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Ristić, Mihailo
AU  - Pešić, Olivera
AU  - Žižović, Irena
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/688
AB  - The present study deals with the supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) of dried bay leaves (Laurus nobilis L.). The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO) from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyzes of the SC-CO2 extract and EO were performed using GC-FID and GC-MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %), principally 1,8-cineole (33.4 %), linalool (16.0 %) and α-terpinyl acetate (13.8 %), sabinene (6.91 %) and methyl eugenol (5.32 %). The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %), together with sesquiterpenes (12.43 %), diterpenes (1.33 %) and esters (31.13 %). The major components were methyl linoleate (16.18 %), α-terpinyl acetate (12.88 %), linalool (9.00 %), methyl eugenol (8.67 %), methyl arachidonate (6.28 %) and eugenol (6.14 %). An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 μg/ml).
AB  - U radu je ispitana nadkritična ekstrakcija i hidrodestilacija osušenih listova lovora (Laurus nobilis L.). Prikazana je uporedna analiza hemijskog sastava i antibakterijske aktivnosti nadkritičnog ekstrakta i etarskog ulja kao i poređenje istih sa literaturnim podacima. Za kvalitativnu i kvantitativnu analizu hemijskog sastava nadkritičnog ekstrakta i etarskog ulja korišćene su GC-FID i GC-MS analitičke metode. Hemijski sastav nadkritičnog ekstrakta i ulja lovora bio je veoma različit. Najzastupljenije komponente u etarskom ulju bili su monoterpeni i njihovi kiseonični derivati (98,4 %), pre svega 1,8-cineol (33,4 %), linalool (16,0 %), α-terpinil-acetat (13,8 %), sabinen (6,91 %) i metil-eugenol (5,32 %). Nadkritični ekstrakt lovora sadržao je dva puta manju količinu monoterpena i njihovih kiseoničnih derivata u odnosu na etarsko ulje (43,89 %) pored seskviterpena (12,43 %), diterpena (1,33 %) i estra (31,13 %). U nadkritičnom ekstraktu najzastupljenije komponente bili su metil-linoleat (16,18 %), α-terpinil-acetat (12,88 %), linalool (9,00 %), metil-eugenol (8,67 %), metil-arahidonat (6,28 %) i eugenol (6,14 %). Antibakterijsko delovanje nadkritičnog ekstrakta i etarskog ulja lovora ispitivano je na sojevima Staphylococcus primenom makrodilucione metode u bujonu. Sojevi Staphylococcus intermedius bili su najosetljiviji na nadkritični ekstrakt i etarsko ulje lovora pri čemu su vrednosti MIC bile 640 μg/ml.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity
T1  - Natkritični ekstrakt i etarsko ulje lovora (Laurus nobilis L.) - hemijski sastav i antibakterijska aktivnost
VL  - 75
IS  - 3
SP  - 395
EP  - 404
DO  - 10.2298/JSC090303003I
ER  - 

@article{
author = "Ivanović, Jasna and Mišić, Dušan and Ristić, Mihailo and Pešić, Olivera and Žižović, Irena",
year = "2010",
abstract = "The present study deals with the supercritical carbon dioxide (SC-CO2) extraction and hydrodistillation (HD) of dried bay leaves (Laurus nobilis L.). The chemical composition and antibacterial activity of the SC-CO2 extract and essential oil (EO) from dried leaves of bay were compared to each other and literature data. Qualitative and quantitative analyzes of the SC-CO2 extract and EO were performed using GC-FID and GC-MS analytical methods. A significant difference in the chemical composition of the SC-CO2 extract and EO was observed. The EO comprised high contents of monoterpenes and their oxygenated derivates (98.4 %), principally 1,8-cineole (33.4 %), linalool (16.0 %) and α-terpinyl acetate (13.8 %), sabinene (6.91 %) and methyl eugenol (5.32 %). The SC-CO2 extract comprised twice less monoterpenes and their oxygenated derivates (43.89 %), together with sesquiterpenes (12.43 %), diterpenes (1.33 %) and esters (31.13 %). The major components were methyl linoleate (16.18 %), α-terpinyl acetate (12.88 %), linalool (9.00 %), methyl eugenol (8.67 %), methyl arachidonate (6.28 %) and eugenol (6.14 %). An investigation of the antibacterial activity of bay SC-CO2 extract and EO was completed on different Staphylococcus strains using the broth macrodilution method. Staphylococcus intermedius strains were the most susceptible to both the SC-CO2 extract and EO (MIC = 640 μg/ml)., U radu je ispitana nadkritična ekstrakcija i hidrodestilacija osušenih listova lovora (Laurus nobilis L.). Prikazana je uporedna analiza hemijskog sastava i antibakterijske aktivnosti nadkritičnog ekstrakta i etarskog ulja kao i poređenje istih sa literaturnim podacima. Za kvalitativnu i kvantitativnu analizu hemijskog sastava nadkritičnog ekstrakta i etarskog ulja korišćene su GC-FID i GC-MS analitičke metode. Hemijski sastav nadkritičnog ekstrakta i ulja lovora bio je veoma različit. Najzastupljenije komponente u etarskom ulju bili su monoterpeni i njihovi kiseonični derivati (98,4 %), pre svega 1,8-cineol (33,4 %), linalool (16,0 %), α-terpinil-acetat (13,8 %), sabinen (6,91 %) i metil-eugenol (5,32 %). Nadkritični ekstrakt lovora sadržao je dva puta manju količinu monoterpena i njihovih kiseoničnih derivata u odnosu na etarsko ulje (43,89 %) pored seskviterpena (12,43 %), diterpena (1,33 %) i estra (31,13 %). U nadkritičnom ekstraktu najzastupljenije komponente bili su metil-linoleat (16,18 %), α-terpinil-acetat (12,88 %), linalool (9,00 %), metil-eugenol (8,67 %), metil-arahidonat (6,28 %) i eugenol (6,14 %). Antibakterijsko delovanje nadkritičnog ekstrakta i etarskog ulja lovora ispitivano je na sojevima Staphylococcus primenom makrodilucione metode u bujonu. Sojevi Staphylococcus intermedius bili su najosetljiviji na nadkritični ekstrakt i etarsko ulje lovora pri čemu su vrednosti MIC bile 640 μg/ml.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity, Natkritični ekstrakt i etarsko ulje lovora (Laurus nobilis L.) - hemijski sastav i antibakterijska aktivnost",
volume = "75",
number = "3",
pages = "395-404",
doi = "10.2298/JSC090303003I"
}

Ivanović, J., Mišić, D., Ristić, M., Pešić, O.,& Žižović, I.. (2010). Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 75(3), 395-404.
https://doi.org/10.2298/JSC090303003I

Ivanović J, Mišić D, Ristić M, Pešić O, Žižović I. Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity. in Journal of the Serbian Chemical Society. 2010;75(3):395-404.
doi:10.2298/JSC090303003I .

Ivanović, Jasna, Mišić, Dušan, Ristić, Mihailo, Pešić, Olivera, Žižović, Irena, "Supercritical CO2 extract and essential oil of bay (Laurus nobilis L.): Chemical composition and antibacterial activity" in Journal of the Serbian Chemical Society, 75, no. 3 (2010):395-404,
https://doi.org/10.2298/JSC090303003I . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/631
AB  - The multiresistance of bacteria to antibiotics, as well as the lack of new antibiotics on the market encouraged the research of antibacterial activity of non-antibiotic substances including plant extracts. During the previous decades, it has been proven that extracts of certain plants have a strong antibacterial activity, but their clinical use was limited due to the presence of organic solvents. However, plant extracts obtained by the process of supercritical fluid extraction contain no traces of solvents, and the latest researches have established that they do have antibacterial effects on some gram-positive bacteria. This comparative study included extracts of Common Mullein, Angelica and Echinacea obtained by means of supercritical fluid extraction, Soxlet extraction and ultrasound-assisted extraction. The study of their antibacterial activity was performed on some strains of Staphylococcus, Enterobacter cloacae and E. coli isolated from clinical material of human and animal origin. A referential strain of S. aureus ATCC 25923 was included in the research. In the study broth macrodilution method was applied by which the MIC values of extracts were determined. The Angelica extract obtained by ultrasound-assisted extraction had the strongest antibacterial activity, i.e. the lowest MIC value of 40 μg/mL for S. epidermidis strain. The Angelica extract obtained by supercritical fluid extraction also showed substantial antibacterial activity to all Staphylococcus strains included in this study, with the MIC values of 320 to 640 μg/mL. The extracts of Echinacea and Common Mullein obtained by supercritical fluid extraction, as well as of Echinacea extract obtained by Soxlet extraction showed no antibacterial activity since the MIC values of these extracts were 2560 μg/mL or >2560 μg/mL for all bacterial strains icluded in the study.
AB  - Multirezistencija na antibiotike bakterija kao i nedostatak novih antibiotika na tržištu lekova podstakao je ispitivanja antibakterijskog delovanja supstancija koje nisu antibiotici uključujući i biljne ekstrakte. Tokom prethodnih decenija, dokazano je da ekstrakti pojedinih biljaka imaju izraženo antibakterijsko delovanje ali je zbog prisustva tragova organskih rastvarača, njihova klinička upotreba ogranič ena. Ekstrakti biljaka dobijeni procesom natkritične ekstrakcije, međutim, nemaju tragove rastvarača, a na osnovu dosadašnjih ispitivanja je utvrđeno da deluju na neke gram-pozitivne bakterije. Ovim komparativinim ispitivanjem obuhvaćeni su ekstrakti divizme, anđelike i ehinacee dobijeni procesima natkritične ekstrakcije, Soxlet-ove ekstrakcije i ultrazvučne ekstrakcije. Ispitivanje njihovog antibakterijskog delovanja vršeno je na nekim sojevima stafilokoka, Enterobacter cloacae i E. coli izolovanim iz uzoraka kliničkog materijala poreklom od životinja i ljudi. U ispitivanje je bio uključen i referentni soj S. aureus ATCC 25923. Za ispitivanje antibakterijskog delovanja ekstrakata primenjen je makrodilucioni metod u bujonu pomoću koga su određivane vrednosti MIC ekstrakata. Najjače antibakterijsko delovanje, odnosno najnižu vrednost MIC od 40 μg/mL za soj S. epidermidis imao je ekstrakt anđelike dobijen ultrazvučnom ekstrakcijom. Ekstrakt anđelike dobijen natkritičnom ekstrakcijom je takođe pokazao značajno antibakterijsko delovanje na sve sojeve stafilokoka obuhvaćenih ispitivanjem sa vrednostima MIC od 320 do 640 μg/mL. Ispitivanjem ekstrakata ehinacee i divizme dobijenih procesom nadkritične ekstrakcije kao i ekstrakta ehinacee dobijenog Soxlet-ovom ekstrakcijom, nije utvrđeno antibakterijsko delovanje jer je vrednost MIC ovih ekstrakata iznosila 2560 μg/mL ili >2560 μg/mL za sojeve bakterija koji su obuhvaćeni ispitivanjem.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals
T1  - Ispitivanje antibakterijskog delovanja natkritičnih ekstrakata biljaka kao i ekstrakata dobijenih drugim tehnološkim procesima na neke bakterije izolovane od životinja
VL  - 59
IS  - 5-6
SP  - 557
EP  - 568
DO  - 10.2298/AVB0906557M
ER  - 

@article{
author = "Mišić, Dušan and Ašanin, Ružica and Ivanović, Jasna and Žižović, Irena",
year = "2009",
abstract = "The multiresistance of bacteria to antibiotics, as well as the lack of new antibiotics on the market encouraged the research of antibacterial activity of non-antibiotic substances including plant extracts. During the previous decades, it has been proven that extracts of certain plants have a strong antibacterial activity, but their clinical use was limited due to the presence of organic solvents. However, plant extracts obtained by the process of supercritical fluid extraction contain no traces of solvents, and the latest researches have established that they do have antibacterial effects on some gram-positive bacteria. This comparative study included extracts of Common Mullein, Angelica and Echinacea obtained by means of supercritical fluid extraction, Soxlet extraction and ultrasound-assisted extraction. The study of their antibacterial activity was performed on some strains of Staphylococcus, Enterobacter cloacae and E. coli isolated from clinical material of human and animal origin. A referential strain of S. aureus ATCC 25923 was included in the research. In the study broth macrodilution method was applied by which the MIC values of extracts were determined. The Angelica extract obtained by ultrasound-assisted extraction had the strongest antibacterial activity, i.e. the lowest MIC value of 40 μg/mL for S. epidermidis strain. The Angelica extract obtained by supercritical fluid extraction also showed substantial antibacterial activity to all Staphylococcus strains included in this study, with the MIC values of 320 to 640 μg/mL. The extracts of Echinacea and Common Mullein obtained by supercritical fluid extraction, as well as of Echinacea extract obtained by Soxlet extraction showed no antibacterial activity since the MIC values of these extracts were 2560 μg/mL or >2560 μg/mL for all bacterial strains icluded in the study., Multirezistencija na antibiotike bakterija kao i nedostatak novih antibiotika na tržištu lekova podstakao je ispitivanja antibakterijskog delovanja supstancija koje nisu antibiotici uključujući i biljne ekstrakte. Tokom prethodnih decenija, dokazano je da ekstrakti pojedinih biljaka imaju izraženo antibakterijsko delovanje ali je zbog prisustva tragova organskih rastvarača, njihova klinička upotreba ogranič ena. Ekstrakti biljaka dobijeni procesom natkritične ekstrakcije, međutim, nemaju tragove rastvarača, a na osnovu dosadašnjih ispitivanja je utvrđeno da deluju na neke gram-pozitivne bakterije. Ovim komparativinim ispitivanjem obuhvaćeni su ekstrakti divizme, anđelike i ehinacee dobijeni procesima natkritične ekstrakcije, Soxlet-ove ekstrakcije i ultrazvučne ekstrakcije. Ispitivanje njihovog antibakterijskog delovanja vršeno je na nekim sojevima stafilokoka, Enterobacter cloacae i E. coli izolovanim iz uzoraka kliničkog materijala poreklom od životinja i ljudi. U ispitivanje je bio uključen i referentni soj S. aureus ATCC 25923. Za ispitivanje antibakterijskog delovanja ekstrakata primenjen je makrodilucioni metod u bujonu pomoću koga su određivane vrednosti MIC ekstrakata. Najjače antibakterijsko delovanje, odnosno najnižu vrednost MIC od 40 μg/mL za soj S. epidermidis imao je ekstrakt anđelike dobijen ultrazvučnom ekstrakcijom. Ekstrakt anđelike dobijen natkritičnom ekstrakcijom je takođe pokazao značajno antibakterijsko delovanje na sve sojeve stafilokoka obuhvaćenih ispitivanjem sa vrednostima MIC od 320 do 640 μg/mL. Ispitivanjem ekstrakata ehinacee i divizme dobijenih procesom nadkritične ekstrakcije kao i ekstrakta ehinacee dobijenog Soxlet-ovom ekstrakcijom, nije utvrđeno antibakterijsko delovanje jer je vrednost MIC ovih ekstrakata iznosila 2560 μg/mL ili >2560 μg/mL za sojeve bakterija koji su obuhvaćeni ispitivanjem.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals, Ispitivanje antibakterijskog delovanja natkritičnih ekstrakata biljaka kao i ekstrakata dobijenih drugim tehnološkim procesima na neke bakterije izolovane od životinja",
volume = "59",
number = "5-6",
pages = "557-568",
doi = "10.2298/AVB0906557M"
}

Mišić, D., Ašanin, R., Ivanović, J.,& Žižović, I.. (2009). Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 557-568.
https://doi.org/10.2298/AVB0906557M

Mišić D, Ašanin R, Ivanović J, Žižović I. Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals. in Acta Veterinaria-Beograd. 2009;59(5-6):557-568.
doi:10.2298/AVB0906557M .

Mišić, Dušan, Ašanin, Ružica, Ivanović, Jasna, Žižović, Irena, "Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):557-568,
https://doi.org/10.2298/AVB0906557M . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Nišavić, Jakov
AU  - Žižović, Irena
AU  - Ivanović, Jasna
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/607
AB  - Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160μg/mL to 1280μg/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 μg/mL to 2560 μg/mL for all examined strains except for 1 strain of S. intermedius of 320 μg/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 μg/mL.
AB  - Ispitivanjem antibakterijskog delovanja natkritičnog ekstrakta timijana (TNKE) i ekstrakta timijana dobijenog hidrodestilacijom (THD) je obuhvaćeno 18 sojeva bakterija iz rodova Staphylococcus, Streptococcus i Enterococus, uključujući sojeve MRSA, sojeve VRE kao i referentne sojeve S.pyogenes ATCC 19615, S.agalactiae ATCC 27959, S.aureus ATCC 11632 i S.aureus ATCC 25923. Antibakterijsko delovanje ekstrakata timijana ispitivano je primenom mikrodilucione metode u bujonu, a citotoksični efekat ekstrakata timijana ispitivan je na ćelijskoj liniji VERO. Dobijene vrednosti MIC THD ekstrakta kretale su se od 160 μg/mL do 1280 μg/mL za sve sojeve bakterija obuhvaćene ispitivanjem. Dobijene vrednost MIC TNKE ekstrakta iznosile su 1280 μg/mL do >2560 μg/mL za sve ispitivane sojeve osim za 1 soj S. intermedius od 320 μg/mL. Citotoksični efekat na ćelijskoj liniji VERO ispitivani ekstrakti su ispolji u koncentracijama višim od 40 μg/mL.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process
T1  - Antibakterijsko delovanje i citotoksični efekat natkritičnog ekstrakta timijana i ekstrakta timijana dobijenog procesom hidrodestilacije
VL  - 63
IS  - 1-2
SP  - 17
EP  - 27
DO  - 10.2298/VETGL0902017M
ER  - 

@article{
author = "Mišić, Dušan and Nišavić, Jakov and Žižović, Irena and Ivanović, Jasna",
year = "2009",
abstract = "Investigations of antibacterial effect of supercritical extraction of thyme (TNKE) and thyme extract obtained by hydrodistillation (THD) covered 18 bacteria strains from the genuses Staphylococcus, Streptococcus and Enterococcus, including the strains MRSA, the strains VRE, as well as the reference strains S.pyogenes ATCC 19615, S. agalactiae ATCC 27959, S. aureus ATCC 11632, and S.aureus ATCC 25923. The anibacterial effect of thyme extracts was examined using the microdillution method in bouillon, and the cytotoxic effect of thyme extract was examined on the VERO cell line. The obtained values for MIC THD extract ranged from 160μg/mL to 1280μg/mL for all bacteria strains covered by the investigations. The obtained values for MIC TNKE extract were 1280 μg/mL to 2560 μg/mL for all examined strains except for 1 strain of S. intermedius of 320 μg/mL. The examined extracts revealed a cytotoxic effect on the VERO cell line in concentrations higher than 40 μg/mL., Ispitivanjem antibakterijskog delovanja natkritičnog ekstrakta timijana (TNKE) i ekstrakta timijana dobijenog hidrodestilacijom (THD) je obuhvaćeno 18 sojeva bakterija iz rodova Staphylococcus, Streptococcus i Enterococus, uključujući sojeve MRSA, sojeve VRE kao i referentne sojeve S.pyogenes ATCC 19615, S.agalactiae ATCC 27959, S.aureus ATCC 11632 i S.aureus ATCC 25923. Antibakterijsko delovanje ekstrakata timijana ispitivano je primenom mikrodilucione metode u bujonu, a citotoksični efekat ekstrakata timijana ispitivan je na ćelijskoj liniji VERO. Dobijene vrednosti MIC THD ekstrakta kretale su se od 160 μg/mL do 1280 μg/mL za sve sojeve bakterija obuhvaćene ispitivanjem. Dobijene vrednost MIC TNKE ekstrakta iznosile su 1280 μg/mL do >2560 μg/mL za sve ispitivane sojeve osim za 1 soj S. intermedius od 320 μg/mL. Citotoksični efekat na ćelijskoj liniji VERO ispitivani ekstrakti su ispolji u koncentracijama višim od 40 μg/mL.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process, Antibakterijsko delovanje i citotoksični efekat natkritičnog ekstrakta timijana i ekstrakta timijana dobijenog procesom hidrodestilacije",
volume = "63",
number = "1-2",
pages = "17-27",
doi = "10.2298/VETGL0902017M"
}

Mišić, D., Nišavić, J., Žižović, I.,& Ivanović, J.. (2009). Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 17-27.
https://doi.org/10.2298/VETGL0902017M

Mišić D, Nišavić J, Žižović I, Ivanović J. Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process. in Veterinarski Glasnik. 2009;63(1-2):17-27.
doi:10.2298/VETGL0902017M .

Mišić, Dušan, Nišavić, Jakov, Žižović, Irena, Ivanović, Jasna, "Antibacterial effect and cytotoxic effect of supercritical extraction of thyme and thyme extract obtained through hydrodistillation process" in Veterinarski Glasnik, 63, no. 1-2 (2009):17-27,
https://doi.org/10.2298/VETGL0902017M . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
AU  - Ivanović, Jasna
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/572
AB  - In the present study, isolation of essential oils from leaves of rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and thyme (Thymus vulgaris) by supercritical carbon dioxide as well as hydrodistillation was investigated from the point of yield and antimicrobial activity of obtained extracts against Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and E.coli strains. Thyme isolates had the highest antibacterial efficiency against tested foodborne bacteria strains (MIC=40-640 μg/cm3) followed by rosemary (MIC=320-1280 μg/cm3) and sage (MIC=160-2560 μg/cm3) isolates. Among tested bacteria, Bacillus species were the most sensitive to presence of all tested supercritical extracts and hydrodistillates. .
AB  - U ovoj studiji je ispitana izolacija etarskih ulja iz lista ruzmarina (Rosmarinus officinalis), žalfije (Salvia officinalis) i timijana (Thymus vulgaris) postupkom natkritične ekstrakcije ugljenik(IV)-oksidom sa aspekta prinosa i antimikrobne aktivnosti dobijenih izolata protiv Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis i E.coli sojeva. Izolati timijana su pokazali najjače antibakterijsko dejstvo u odnosu na testirane sojeve (MIC=40-640 μg/cm3). Zatim slede izolati ruzmarina (MIC=320-1280 μg/cm3) i žalfije (MIC=160-2560 μg/cm3). Među testiranim bakterijama, Bacillus sojevi su se pokazali kao najosetljiviji na prisustvo kako natkritičnih ekstrakata, tako i hidrodestilata. .
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods
T1  - Antibakterijska aktivnost etarskih ulja odabranih biljaka familije Lamiaceae izolovanih različitim metodama
IS  - 19
SP  - 20
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_572
ER  - 

@article{
author = "Žižović, Irena and Mišić, Dušan and Ašanin, Ružica and Ivanović, Jasna",
year = "2009",
abstract = "In the present study, isolation of essential oils from leaves of rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and thyme (Thymus vulgaris) by supercritical carbon dioxide as well as hydrodistillation was investigated from the point of yield and antimicrobial activity of obtained extracts against Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and E.coli strains. Thyme isolates had the highest antibacterial efficiency against tested foodborne bacteria strains (MIC=40-640 μg/cm3) followed by rosemary (MIC=320-1280 μg/cm3) and sage (MIC=160-2560 μg/cm3) isolates. Among tested bacteria, Bacillus species were the most sensitive to presence of all tested supercritical extracts and hydrodistillates. ., U ovoj studiji je ispitana izolacija etarskih ulja iz lista ruzmarina (Rosmarinus officinalis), žalfije (Salvia officinalis) i timijana (Thymus vulgaris) postupkom natkritične ekstrakcije ugljenik(IV)-oksidom sa aspekta prinosa i antimikrobne aktivnosti dobijenih izolata protiv Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis i E.coli sojeva. Izolati timijana su pokazali najjače antibakterijsko dejstvo u odnosu na testirane sojeve (MIC=40-640 μg/cm3). Zatim slede izolati ruzmarina (MIC=320-1280 μg/cm3) i žalfije (MIC=160-2560 μg/cm3). Među testiranim bakterijama, Bacillus sojevi su se pokazali kao najosetljiviji na prisustvo kako natkritičnih ekstrakata, tako i hidrodestilata. .",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods, Antibakterijska aktivnost etarskih ulja odabranih biljaka familije Lamiaceae izolovanih različitim metodama",
number = "19",
pages = "20-26",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_572"
}

Žižović, I., Mišić, D., Ašanin, R.,& Ivanović, J.. (2009). Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods. in Zbornik radova Tehnološkog fakulteta, Leskovac
Univerzitet u Nišu - Tehnološki fakultet, Leskovac.(19), 20-26.
https://hdl.handle.net/21.15107/rcub_veterinar_572

Žižović I, Mišić D, Ašanin R, Ivanović J. Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2009;(19):20-26.
https://hdl.handle.net/21.15107/rcub_veterinar_572 .

Žižović, Irena, Mišić, Dušan, Ašanin, Ružica, Ivanović, Jasna, "Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 19 (2009):20-26,
https://hdl.handle.net/21.15107/rcub_veterinar_572 .