Todorović, Dalibor

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orcid::0000-0001-6707-9292
  • Todorović, Dalibor (10)

Author's Bibliography

Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia

Pajić, Marko; Todorović, Dalibor; Knežević, Slobodan; Prunić, Bojana; Velhner, Maja; Ostojić Andrić, Dušica; Stanimirović, Zoran

(MDPI, 2023)

TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Knežević, Slobodan
AU  - Prunić, Bojana
AU  - Velhner, Maja
AU  - Ostojić Andrić, Dušica
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2902
AB  - Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.
PB  - MDPI
T2  - Life
T1  - Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia
VL  - 13
IS  - 4
SP  - 1
EP  - 13
DO  - 10.3390/life13041039
ER  - 
@article{
author = "Pajić, Marko and Todorović, Dalibor and Knežević, Slobodan and Prunić, Bojana and Velhner, Maja and Ostojić Andrić, Dušica and Stanimirović, Zoran",
year = "2023",
abstract = "Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.",
publisher = "MDPI",
journal = "Life",
title = "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia",
volume = "13",
number = "4",
pages = "1-13",
doi = "10.3390/life13041039"
}
Pajić, M., Todorović, D., Knežević, S., Prunić, B., Velhner, M., Ostojić Andrić, D.,& Stanimirović, Z.. (2023). Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life
MDPI., 13(4), 1-13.
https://doi.org/10.3390/life13041039
Pajić M, Todorović D, Knežević S, Prunić B, Velhner M, Ostojić Andrić D, Stanimirović Z. Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life. 2023;13(4):1-13.
doi:10.3390/life13041039 .
Pajić, Marko, Todorović, Dalibor, Knežević, Slobodan, Prunić, Bojana, Velhner, Maja, Ostojić Andrić, Dušica, Stanimirović, Zoran, "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia" in Life, 13, no. 4 (2023):1-13,
https://doi.org/10.3390/life13041039 . .
1
6

Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji

Todorović, Dalibor; Kozoderović, Gordana; Pajić, Marko; Đurđević, Biljana; Ljubojević Pelić, Dragana; Marjanović, Đorđe; Velhner, Maja

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2023)

TY  - CONF
AU  - Todorović, Dalibor
AU  - Kozoderović, Gordana
AU  - Pajić, Marko
AU  - Đurđević, Biljana
AU  - Ljubojević Pelić, Dragana
AU  - Marjanović, Đorđe
AU  - Velhner, Maja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3347
AB  - Коменсалне E. coli у цревима дивљих животиња нису изложене честим
директним антимикробним притисцима, као што је случај са коменсалним E.
coli у цревима домаћих, посебно фармских животиња и кућних љубимаца.
Резистенција ових бактерија код дивљих животиња настала је индиректно,
односно човековим немаром и загађењем животне средине, чиме су бактерије
отпорне на антибиотике доспеле у ланац исхране дивљих животиња. Као
последица тога, у бактеријској цревној флори дивљих животиња и птица, ипак
постоји селективни притисак, који је одговоран за перзистенцију гена
резистенције. У овом раду су приказани резултати истраживања резистенције
индикаторске E. coli на флуорохинолоне, које је у последњих пет година
спровела Национална референтна лабораторија за антимикорбну резистенцију
у ветеринарској медицини у Србији. Детектован је висок ниво резистенције на
флуорохинолоне коменсалне E. coli код дивљих свиња, зечева, срна и јелена у
ловиштима на територији Аутономне покрајине Војводине. Oве бактерије су
поред хромозомске резистенције имале и плазмидски преносиву резистенцију
на флуорохинолоне (PMQR). Резистенција на флуорохинолоне је откривена и
код коменсалне E. coli од орлова белорепана у Специјалном резревату природе
„Горње Подунавље“, делу резервата биосфере „Бачко Подунавље“, које се
налази на УНЕСКО-вој светској листи резервата биосфере. Такође,
резистенција коменсалне E. coli на флуорохинолоне је детектована и код
црноглавих галебова који се хране на депонији града Новог Сада.
Забрињавајући је висок ниво резистенције на синтетске антибиотике, тј. на
флуорохинолне у популацији дивљих животиња и птица јер ови антибиотици
често представљају лек избора у терапији одређених бактеријских инфекција
људи и домаћих животиња. Дивље животиње и птице су потенцијални
резервоари гена резистенције и могу бити одговорне за ширење резистенције у
бактеријским заједницама на нашој планети.
AB  - Commensal E. coli in the intestines of wild animals are not exposed to frequent direct
antimicrobial pressure, as is the case with commensal E. coli in the intestines of
domestic, especially farm animals and pets. The resistance of these bacteria in wild
animals was caused indirectly, that is, by human negligence and environmental
pollution, which allowed antibiotic-resistant bacteria to enter the food chain of wild
animals and birds. Consequently, in the bacterial intestinal flora of wild animals and
birds, there is nevertheless a selective pressure, which is responsible for the
persistence of resistance genes. This paper presents the results of the research on the
resistance of indicator E. coli to fluoroquinolones, which was conducted in the last
five years by the National Reference Laboratory for Antimicrobial Resistance in
Veterinary Medicine in Serbia. A high level of resistance to fluoroquinolones of
commensal E. coli was detected in wild boars, rabbits, roe deer and deer in hunting
grounds on the territory of the Province of Vojvodina. In addition to chromosomal
resistance, these bacteria also had plasmid-mediated resistance to fluoroquinolones
(PMQR). Resistance to fluoroquinolones was also discovered in commensal E. coli
from white-tailed eagles in the Gornje Podunavlje Special Natural Reserve, a part of
Bačko Podunavlje Biosphere Reserve, approved as a UNESCO Biosphere Reserve.
Also, resistance to fluoroquinolones of commensal E. coli was detected in Blackheaded gulls that feeding at the landfill of the city of Novi Sad. Very worrying the
high level of resistance to synthetic antibiotics, ie. to fluoroquinolone in the
population of wild animals and birds because these antibiotics are often the drug of
choice in the treatment of certain bacterial infections of humans and domestic animals.
Wild animals and birds are potential reservoirs of resistance genes and may be
responsible for dissemination of resistance in bacterial communities on our planet.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji
T1  - High level of resistance to fluoroquinolones in commensal Escherichia coli isolated from wild animals and birds in Serbia
SP  - 182
EP  - 183
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3347
ER  - 
@conference{
author = "Todorović, Dalibor and Kozoderović, Gordana and Pajić, Marko and Đurđević, Biljana and Ljubojević Pelić, Dragana and Marjanović, Đorđe and Velhner, Maja",
year = "2023",
abstract = "Коменсалне E. coli у цревима дивљих животиња нису изложене честим
директним антимикробним притисцима, као што је случај са коменсалним E.
coli у цревима домаћих, посебно фармских животиња и кућних љубимаца.
Резистенција ових бактерија код дивљих животиња настала је индиректно,
односно човековим немаром и загађењем животне средине, чиме су бактерије
отпорне на антибиотике доспеле у ланац исхране дивљих животиња. Као
последица тога, у бактеријској цревној флори дивљих животиња и птица, ипак
постоји селективни притисак, који је одговоран за перзистенцију гена
резистенције. У овом раду су приказани резултати истраживања резистенције
индикаторске E. coli на флуорохинолоне, које је у последњих пет година
спровела Национална референтна лабораторија за антимикорбну резистенцију
у ветеринарској медицини у Србији. Детектован је висок ниво резистенције на
флуорохинолоне коменсалне E. coli код дивљих свиња, зечева, срна и јелена у
ловиштима на територији Аутономне покрајине Војводине. Oве бактерије су
поред хромозомске резистенције имале и плазмидски преносиву резистенцију
на флуорохинолоне (PMQR). Резистенција на флуорохинолоне је откривена и
код коменсалне E. coli од орлова белорепана у Специјалном резревату природе
„Горње Подунавље“, делу резервата биосфере „Бачко Подунавље“, које се
налази на УНЕСКО-вој светској листи резервата биосфере. Такође,
резистенција коменсалне E. coli на флуорохинолоне је детектована и код
црноглавих галебова који се хране на депонији града Новог Сада.
Забрињавајући је висок ниво резистенције на синтетске антибиотике, тј. на
флуорохинолне у популацији дивљих животиња и птица јер ови антибиотици
често представљају лек избора у терапији одређених бактеријских инфекција
људи и домаћих животиња. Дивље животиње и птице су потенцијални
резервоари гена резистенције и могу бити одговорне за ширење резистенције у
бактеријским заједницама на нашој планети., Commensal E. coli in the intestines of wild animals are not exposed to frequent direct
antimicrobial pressure, as is the case with commensal E. coli in the intestines of
domestic, especially farm animals and pets. The resistance of these bacteria in wild
animals was caused indirectly, that is, by human negligence and environmental
pollution, which allowed antibiotic-resistant bacteria to enter the food chain of wild
animals and birds. Consequently, in the bacterial intestinal flora of wild animals and
birds, there is nevertheless a selective pressure, which is responsible for the
persistence of resistance genes. This paper presents the results of the research on the
resistance of indicator E. coli to fluoroquinolones, which was conducted in the last
five years by the National Reference Laboratory for Antimicrobial Resistance in
Veterinary Medicine in Serbia. A high level of resistance to fluoroquinolones of
commensal E. coli was detected in wild boars, rabbits, roe deer and deer in hunting
grounds on the territory of the Province of Vojvodina. In addition to chromosomal
resistance, these bacteria also had plasmid-mediated resistance to fluoroquinolones
(PMQR). Resistance to fluoroquinolones was also discovered in commensal E. coli
from white-tailed eagles in the Gornje Podunavlje Special Natural Reserve, a part of
Bačko Podunavlje Biosphere Reserve, approved as a UNESCO Biosphere Reserve.
Also, resistance to fluoroquinolones of commensal E. coli was detected in Blackheaded gulls that feeding at the landfill of the city of Novi Sad. Very worrying the
high level of resistance to synthetic antibiotics, ie. to fluoroquinolone in the
population of wild animals and birds because these antibiotics are often the drug of
choice in the treatment of certain bacterial infections of humans and domestic animals.
Wild animals and birds are potential reservoirs of resistance genes and may be
responsible for dissemination of resistance in bacterial communities on our planet.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji, High level of resistance to fluoroquinolones in commensal Escherichia coli isolated from wild animals and birds in Serbia",
pages = "182-183",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3347"
}
Todorović, D., Kozoderović, G., Pajić, M., Đurđević, B., Ljubojević Pelić, D., Marjanović, Đ.,& Velhner, M.. (2023). Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3347
Todorović D, Kozoderović G, Pajić M, Đurđević B, Ljubojević Pelić D, Marjanović Đ, Velhner M. Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:182-183.
https://hdl.handle.net/21.15107/rcub_veterinar_3347 .
Todorović, Dalibor, Kozoderović, Gordana, Pajić, Marko, Đurđević, Biljana, Ljubojević Pelić, Dragana, Marjanović, Đorđe, Velhner, Maja, "Visok nivo rezistencije na fluorohinolone komensalnih Escherichia coli izolovanih o divljih životinja i ptica u Srbiji" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):182-183,
https://hdl.handle.net/21.15107/rcub_veterinar_3347 .

Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries

Todorović, Dalibor; Pajić, Marko; Marjanović, Đorđe; Ljubojević Pelić, Dragana; Vidaković Knežević, Suzana; Đurđević, Biljana; Velhner, Maja

(University of Novi Sad, Faculty of Agriculture, 2022)

TY  - CONF
AU  - Todorović, Dalibor
AU  - Pajić, Marko
AU  - Marjanović, Đorđe
AU  - Ljubojević Pelić, Dragana
AU  - Vidaković Knežević, Suzana
AU  - Đurđević, Biljana
AU  - Velhner, Maja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3348
AB  - Monitoring of antimicrobial resistance is mandatory in all EU member states
and is carried out according to the Decision of the European Commission No.
2020/1729. Member states are required to test annually the resistance of 170 isolates
of Escehrichia coli, Salmonella spp., Campylobacter jejuni/coli and Enterococcus
faecalis/fecium, by determining the minimum inhibitory concentration of antibiotic
that inhibits bacterial growth (MIC). Also, they are obliged to test 300 isolates of
Escherichia coli and Salmonella spp. for the production of ESBL, AmpC, or CP
enzymes. Monitoring shall be carried out in accordance with the procedures described
by the European Committee for Antimicrobial Susceptibility Testing (EUCAST),
according to the international reference method ISO 20776-1: 2021 (E) entitled
"Susceptibility testing of infectious agents and evaluation of performance of
antimicrobial susceptibility test devices - Part 1: Broth micro-dilution reference
method for testing the in vitro activity of antimicrobial agents against rapidly growing
aerobic bacteria involved in infectious diseases". Directive EU2020/1729 stipulates
that isolates should come from healthy animals, so for these reasons, sampling at the
slaughter line in slaughterhouses, retail outlets, as well as on disease-free farms is
most appropriate.
PB  - University of Novi Sad, Faculty of Agriculture
C3  - Conference  Antimicrobial Resistance in Veterinary Medicine-Current State and Perspectives, Novi Sad, 21 - 23. jun 2022
T1  - Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries
SP  - 141
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3348
ER  - 
@conference{
author = "Todorović, Dalibor and Pajić, Marko and Marjanović, Đorđe and Ljubojević Pelić, Dragana and Vidaković Knežević, Suzana and Đurđević, Biljana and Velhner, Maja",
year = "2022",
abstract = "Monitoring of antimicrobial resistance is mandatory in all EU member states
and is carried out according to the Decision of the European Commission No.
2020/1729. Member states are required to test annually the resistance of 170 isolates
of Escehrichia coli, Salmonella spp., Campylobacter jejuni/coli and Enterococcus
faecalis/fecium, by determining the minimum inhibitory concentration of antibiotic
that inhibits bacterial growth (MIC). Also, they are obliged to test 300 isolates of
Escherichia coli and Salmonella spp. for the production of ESBL, AmpC, or CP
enzymes. Monitoring shall be carried out in accordance with the procedures described
by the European Committee for Antimicrobial Susceptibility Testing (EUCAST),
according to the international reference method ISO 20776-1: 2021 (E) entitled
"Susceptibility testing of infectious agents and evaluation of performance of
antimicrobial susceptibility test devices - Part 1: Broth micro-dilution reference
method for testing the in vitro activity of antimicrobial agents against rapidly growing
aerobic bacteria involved in infectious diseases". Directive EU2020/1729 stipulates
that isolates should come from healthy animals, so for these reasons, sampling at the
slaughter line in slaughterhouses, retail outlets, as well as on disease-free farms is
most appropriate.",
publisher = "University of Novi Sad, Faculty of Agriculture",
journal = "Conference  Antimicrobial Resistance in Veterinary Medicine-Current State and Perspectives, Novi Sad, 21 - 23. jun 2022",
title = "Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries",
pages = "141-147",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3348"
}
Todorović, D., Pajić, M., Marjanović, Đ., Ljubojević Pelić, D., Vidaković Knežević, S., Đurđević, B.,& Velhner, M.. (2022). Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries. in Conference  Antimicrobial Resistance in Veterinary Medicine-Current State and Perspectives, Novi Sad, 21 - 23. jun 2022
University of Novi Sad, Faculty of Agriculture., 141-147.
https://hdl.handle.net/21.15107/rcub_veterinar_3348
Todorović D, Pajić M, Marjanović Đ, Ljubojević Pelić D, Vidaković Knežević S, Đurđević B, Velhner M. Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries. in Conference  Antimicrobial Resistance in Veterinary Medicine-Current State and Perspectives, Novi Sad, 21 - 23. jun 2022. 2022;:141-147.
https://hdl.handle.net/21.15107/rcub_veterinar_3348 .
Todorović, Dalibor, Pajić, Marko, Marjanović, Đorđe, Ljubojević Pelić, Dragana, Vidaković Knežević, Suzana, Đurđević, Biljana, Velhner, Maja, "Monitoring of antimicrobial resistance under directive 2020/1729 in EU countries" in Conference  Antimicrobial Resistance in Veterinary Medicine-Current State and Perspectives, Novi Sad, 21 - 23. jun 2022 (2022):141-147,
https://hdl.handle.net/21.15107/rcub_veterinar_3348 .

Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema

Bugarski, Dejan; Petrović, Tamaš; Lupulović, Diana; Lazić, Sava; Todorović, Dalibor; Savić, Sara; Bojkovski, Jovan

(Beograd : Srpsko veterinarsko društvo, 2020)

TY  - CONF
AU  - Bugarski, Dejan
AU  - Petrović, Tamaš
AU  - Lupulović, Diana
AU  - Lazić, Sava
AU  - Todorović, Dalibor
AU  - Savić, Sara
AU  - Bojkovski, Jovan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3520
AB  - Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
T1  - Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema
SP  - 51
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3520
ER  - 
@conference{
author = "Bugarski, Dejan and Petrović, Tamaš and Lupulović, Diana and Lazić, Sava and Todorović, Dalibor and Savić, Sara and Bojkovski, Jovan",
year = "2020",
abstract = "Инфекције дисајних органа говеда са својом мултифакторијалном етиологијом су сталан
проблем говедарске производње и истовремено изазов за ветеринарску струку. Најугроженије
узрасне и производне групе говеда, на фармама крава су телад старости до 4 месеца, а у
товилиштима телад на почетку това, најчешће у тежини између 100 и 250kg. На морбидитет, а
касније и на морталитет утичу услови држања, поступак са животињама, имуни статус,
изложеност микроорганизмима, врста присутних патогених микроорганизама.
Мултифакторијалност узрока овог обољења отежава постизање потпуне профилаксе те се пре у
овом случају може говорити о предузимању мера за смањење ризика и свођење трошкова које
узрокује обољење у прихватљив оквир. Штете које инфекције дисајних органа говеда наносе су
најизраженије у тову говеда. Са ветеринарског становишта, спровођење имунопрофилаксе се
сматра најделотворнијом и најлакшом методом у сузбијању респираторних инфекција говеда.
Међутим, у пракси се она не ретко показује као непотпуно успешна што доводи до незадовољства
власника животиња и његовог удаљавања од ветеринара. Узроци неуспеха спроведене
имунопрофилаксе су неподударање микрорганизама који су довели до инфекције и
микроорганизама чији су антигени коришћени приликом имунизације, затим статус животиње у
погледу инфекције приликом имунизације, могућност имуног одговора имунизоване јединке,
време имунизације с обзиром на технолошки процес узгоја и друго. Запажено је да се мења
приступ појединим микроорганизмима у односу на њихов значај. Испитивања у свету и у нашој
земљи указују на промене у етиологији, нарочито када су питању вируси. Питање је да ли су
промене у етиологији и оцени значаја стварне или се само услед побољшаних дијагностичких
могућности открива значај појединих узрочника који су раније били занемарени. Седамдесетих и
осамдесетих година прошлог века су се најзначајнијим вирусним узрочницима сматрали говеђи
херпес вирус 1 и вирус параинфлуенце 3, касније значај преузимају вирус дијареје говеда, говеђи
респираторни синцицијални вирус, а у новије време све више се запажа значај корона вируса
говеда. Истовремено, Mycoplasma bovis се такође почиње сматрати значајним узрочником
инфекција дисајних органа говеда. Бактеријске инфекције плућа су редован пратилац вирусних
инфекција, али у одређеним условима могу самостално да нанесу велику штету. Због тога се
сматра да је примена антибиотика неопходна и они се, када су у питању инфекције плућа, користе
поред терапијске и у профилактичку и метафилактичку сврху што повлачи за собом одговорност
ветеринара за контролисаном и одговорном употребом.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020",
title = "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema",
pages = "51-62",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3520"
}
Bugarski, D., Petrović, T., Lupulović, D., Lazić, S., Todorović, D., Savić, S.,& Bojkovski, J.. (2020). Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020
Beograd : Srpsko veterinarsko društvo., 51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520
Bugarski D, Petrović T, Lupulović D, Lazić S, Todorović D, Savić S, Bojkovski J. Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema. in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020. 2020;:51-62.
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .
Bugarski, Dejan, Petrović, Tamaš, Lupulović, Diana, Lazić, Sava, Todorović, Dalibor, Savić, Sara, Bojkovski, Jovan, "Respiratorne infekcije goveda – najčešći uzroci i mogućnosti za rešavanje problema" in 31. Savetovanje veterinara Srbije, Online Webinar, 10 - 13. septembar 2020 (2020):51-62,
https://hdl.handle.net/21.15107/rcub_veterinar_3520 .

Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia

Todorović, Dalibor; Velhner, Maja; Grego, Edita; Vidanović, Dejan; Milanov, Dubravka; Krnjaić, Dejan; Kehrenberg, Corinna

(Mary Ann Liebert, Inc, New Rochelle, 2018)

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Grego, Edita
AU  - Vidanović, Dejan
AU  - Milanov, Dubravka
AU  - Krnjaić, Dejan
AU  - Kehrenberg, Corinna
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1648
AB  - The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia
VL  - 24
IS  - 1
SP  - 95
EP  - 103
DO  - 10.1089/mdr.2017.0016
ER  - 
@article{
author = "Todorović, Dalibor and Velhner, Maja and Grego, Edita and Vidanović, Dejan and Milanov, Dubravka and Krnjaić, Dejan and Kehrenberg, Corinna",
year = "2018",
abstract = "The aim of the study was to characterize multidrug-resistant (MDR) Escherichia coli isolates collected in Serbia from bovine clinical mastitis cases and diseased pigs, mainly with molecular methods. A total of 48 E. coli isolates was collected during the years 2013-2014, of which 22 were MDR and were included in further analysis. Phylogenetic typing showed that 17 isolates belonged to group A, while two isolates were classified in group B1 and a single one in group D. All isolates showed unique macrorestriction patterns. Phenotypic susceptibility testing revealed resistances of the isolates against up to 13 antimicrobial agents, including resistance to fluoroquinolones. A wide variety of resistance genes was detected by PCR amplification and sequencing of amplicons. Sequence analysis of the quinolone resistance determining regions of topoisomerase genes revealed mutations in gyrA, parC, and/or parE. Plasmid-mediated quinolone resistance genes were detected in two porcine (aac-6-Ib-cr and qnrS, respectively) isolates and a single bovine (aac-6-Ib-cr) isolate. Resistance genes were found to be located on conjugative plasmids in 16 cases, many of which conferred a multidrug resistance phenotype. In conclusion, the plentitude of resistance genes located on conjugative plasmids and integrons in E. coli from cows and pigs in Vojvodina, Serbia, pose a high risk for horizontal gene transfer in bacteria from livestock husbandry.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia",
volume = "24",
number = "1",
pages = "95-103",
doi = "10.1089/mdr.2017.0016"
}
Todorović, D., Velhner, M., Grego, E., Vidanović, D., Milanov, D., Krnjaić, D.,& Kehrenberg, C.. (2018). Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 24(1), 95-103.
https://doi.org/10.1089/mdr.2017.0016
Todorović D, Velhner M, Grego E, Vidanović D, Milanov D, Krnjaić D, Kehrenberg C. Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia. in Microbial Drug Resistance. 2018;24(1):95-103.
doi:10.1089/mdr.2017.0016 .
Todorović, Dalibor, Velhner, Maja, Grego, Edita, Vidanović, Dejan, Milanov, Dubravka, Krnjaić, Dejan, Kehrenberg, Corinna, "Molecular Characterization of Multidrug-Resistant Escherichia coli Isolates from Bovine Clinical Mastitis and Pigs in the Vojvodina Province, Serbia" in Microbial Drug Resistance, 24, no. 1 (2018):95-103,
https://doi.org/10.1089/mdr.2017.0016 . .
1
15
10
16

Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals

Milanov, Dubravka; Petrović, Tamaš; Todorović, Dalibor; Aleksić, Nevenka; Čabarkapa, Ivana

(Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad, 2018)

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Petrović, Tamaš
AU  - Todorović, Dalibor
AU  - Aleksić, Nevenka
AU  - Čabarkapa, Ivana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1642
AB  - Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed.
AB  - Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.
PB  - Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad
T2  - Food and Feed Research
T1  - Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals
T1  - Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja
VL  - 45
IS  - 1
SP  - 67
EP  - 76
DO  - 10.5937/FFR1801067M
ER  - 
@article{
author = "Milanov, Dubravka and Petrović, Tamaš and Todorović, Dalibor and Aleksić, Nevenka and Čabarkapa, Ivana",
year = "2018",
abstract = "Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins α and β-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed., Clostridium perfringens je Gram-pozitivna, anaerobna, sporulišuća, štapićasta bakterija, ubikvitarno rasprostranjena u prirodi. Kod različitih sojeva C. Perfringens, do danas, identifikovano je oko 17 vrsta toksina. C. perfringens je uzročni agens različitih oboljenja (sindroma), ali su crevne infekcije/intoksikacije najčešće i od najvećeg značaja za zdravlje farmski gajenih životinja. U ovom radu prikazujemo rezultate ispitivanja sojeva C. perfringens poreklom iz hrane za životinje (n=34) i jednog izolata iz organa krave uginule sa znacima enterotoksemije na prisustvo gena: cpa, cpb, cpb2, cpe, etx i iap primenom multipleks PCR tehnike. Svi sojevi C. perfringens izolovani iz hrane za životinje, identifikovani su kao tip A koji poseduje samo cpa gen (n=21) ili tip A koji produkuje b2-toksin, odnosno ima cpa i cpb2 gene (n=13). U radu diskutujemo o ulozi alfa (α) i beta-2 (β2) toksina u patogenezi enterotoksemija domaćih životinja, kao i aktuelnom zakonskom propisu po kojem ova vrsta bakterije ne sme biti prisutna u hrani za životinje. Primena PCR tehnike u svakodnevnoj praksi omogućila bi toksin-genotipizaciju sojeva C. perfringens, a time i realne osnove za uspostavljanje graničnih dozvoljenih vrednosti za ovu vrstu bakterije u hrani za životinje.",
publisher = "Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad",
journal = "Food and Feed Research",
title = "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals, Toksin genotipizacija sojeva Clostridium perfringens izolovanih iz hrane za životinje i njihov značaj u etiologiji enterotoksemija domaćih životinja",
volume = "45",
number = "1",
pages = "67-76",
doi = "10.5937/FFR1801067M"
}
Milanov, D., Petrović, T., Todorović, D., Aleksić, N.,& Čabarkapa, I.. (2018). Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research
Univerzitet u Novom Sadu - Naučni institut za prehrambene tehnologije, Novi Sad., 45(1), 67-76.
https://doi.org/10.5937/FFR1801067M
Milanov D, Petrović T, Todorović D, Aleksić N, Čabarkapa I. Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals. in Food and Feed Research. 2018;45(1):67-76.
doi:10.5937/FFR1801067M .
Milanov, Dubravka, Petrović, Tamaš, Todorović, Dalibor, Aleksić, Nevenka, Čabarkapa, Ivana, "Toxin genotypes of Clostridium perfringens in animal feed and their role in the ethiology of enterotoxemia in domestic animals" in Food and Feed Research, 45, no. 1 (2018):67-76,
https://doi.org/10.5937/FFR1801067M . .
6

Pasteurella multocida mastitis in cow: Case report

Milanov, Dubravka; Aleksić, Nevenka; Todorović, Dalibor; Bugarski, Dejan

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2017)

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Aleksić, Nevenka
AU  - Todorović, Dalibor
AU  - Bugarski, Dejan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1448
AB  - Pasteurella (P.) multocida is a heterogeneous species of Gram-negative bacteria which are common commensals of the upper respiratory system of various mammal and bird species, but are also opportunistic contagious zoonotic pathogens which cause a wide spectre of infections in domestic animals and humans. P. multocida is a rare cause of mastitis in dairy cows. The source of infection mainly remains unknown, mastitis usually is acute, and the therapy by intramammary administration of antibiotics does not lead to satisfactory results. Lethality is possible due to presence of endotoxins in blood. Literature data on P. multocida mastitis in dairy cows is particularly scarce, which is why such a case is described in the current work, with past medical history, clinical findings, laboratory diagnostics and therapeutic approach.
AB  - Pasteurella multocida je heterogena vrsta gram negativnih bakterija koje su uobičajeni komensali prednjih partija respiratornog sistema različitih vrsta sisara i ptica, ali i oportunistički, kontagiozni i zoonotski patogeni, koji izazivaju širok spektar infekcija kod domaćih životinja i ljudi. P. multocida spada u retke uzročnike mastitisa mlečnih krava. Izvor infekcije za mlečnu žlezdu uglavnom ostaje nepoznat, mastitis obično ima akutni tok, a terapija intramamarnom aplikacijom antibiotika ne daje zadovoljavajuće rezultate. Letalni ishod je moguć usled razvoja endotoksemije. U literaturi ima veoma malo podataka o mastitisima mlečnih krava čiji je uzročnik P. multocida, zbog čega u ovom radu opisujemo jedan takav slučaj, uz prikaz osnovnih anamnestičkih podataka, kliničke slike, laboratorijske dijagnostike i terapijskog pristupa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Pasteurella multocida mastitis in cow: Case report
T1  - Mastitis kod krave izazvan bakterijom Pasteurella multocida
VL  - 71
IS  - 2
SP  - 117
EP  - 122
DO  - 10.2298/VETGL170619011M
ER  - 
@article{
author = "Milanov, Dubravka and Aleksić, Nevenka and Todorović, Dalibor and Bugarski, Dejan",
year = "2017",
abstract = "Pasteurella (P.) multocida is a heterogeneous species of Gram-negative bacteria which are common commensals of the upper respiratory system of various mammal and bird species, but are also opportunistic contagious zoonotic pathogens which cause a wide spectre of infections in domestic animals and humans. P. multocida is a rare cause of mastitis in dairy cows. The source of infection mainly remains unknown, mastitis usually is acute, and the therapy by intramammary administration of antibiotics does not lead to satisfactory results. Lethality is possible due to presence of endotoxins in blood. Literature data on P. multocida mastitis in dairy cows is particularly scarce, which is why such a case is described in the current work, with past medical history, clinical findings, laboratory diagnostics and therapeutic approach., Pasteurella multocida je heterogena vrsta gram negativnih bakterija koje su uobičajeni komensali prednjih partija respiratornog sistema različitih vrsta sisara i ptica, ali i oportunistički, kontagiozni i zoonotski patogeni, koji izazivaju širok spektar infekcija kod domaćih životinja i ljudi. P. multocida spada u retke uzročnike mastitisa mlečnih krava. Izvor infekcije za mlečnu žlezdu uglavnom ostaje nepoznat, mastitis obično ima akutni tok, a terapija intramamarnom aplikacijom antibiotika ne daje zadovoljavajuće rezultate. Letalni ishod je moguć usled razvoja endotoksemije. U literaturi ima veoma malo podataka o mastitisima mlečnih krava čiji je uzročnik P. multocida, zbog čega u ovom radu opisujemo jedan takav slučaj, uz prikaz osnovnih anamnestičkih podataka, kliničke slike, laboratorijske dijagnostike i terapijskog pristupa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Pasteurella multocida mastitis in cow: Case report, Mastitis kod krave izazvan bakterijom Pasteurella multocida",
volume = "71",
number = "2",
pages = "117-122",
doi = "10.2298/VETGL170619011M"
}
Milanov, D., Aleksić, N., Todorović, D.,& Bugarski, D.. (2017). Pasteurella multocida mastitis in cow: Case report. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 117-122.
https://doi.org/10.2298/VETGL170619011M
Milanov D, Aleksić N, Todorović D, Bugarski D. Pasteurella multocida mastitis in cow: Case report. in Veterinarski Glasnik. 2017;71(2):117-122.
doi:10.2298/VETGL170619011M .
Milanov, Dubravka, Aleksić, Nevenka, Todorović, Dalibor, Bugarski, Dejan, "Pasteurella multocida mastitis in cow: Case report" in Veterinarski Glasnik, 71, no. 2 (2017):117-122,
https://doi.org/10.2298/VETGL170619011M . .
2

Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia

Todorović, Dalibor; Velhner, Maja; Milanov, Dubravka; Vidanović, Dejan; Suvajdzić, Ljiljana; Stojanov, Igor; Krnjaić, Dejan

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2015)

TY  - JOUR
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Vidanović, Dejan
AU  - Suvajdzić, Ljiljana
AU  - Stojanov, Igor
AU  - Krnjaić, Dejan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1191
AB  - Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia
VL  - 65
IS  - 4
SP  - 548
EP  - 556
DO  - 10.1515/acve-2015-0046
ER  - 
@article{
author = "Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Vidanović, Dejan and Suvajdzić, Ljiljana and Stojanov, Igor and Krnjaić, Dejan",
year = "2015",
abstract = "Resistance to tetracycline was studied in Salmonella Infantis isolated from 28 poultry farms in the Northern part of Serbia (The Autonomous Province of Vojvodina). A total of 18 isolates were resistant to nalidixic acid (NAL) and tetracycline (TET). The minimal inhibitory concentration (MIC) to TET, ranged from 1-256 mg/L. Namely, 13 isolates exhibited MIC to TET at 256 mg/L, in four of the isolates, the MIC was 128 mg/L and one isolate had MIC 64 mg/L. Ten isolates were exhibiting a MIC of 1mg/L. It was evident that Salmonella Infantis had also spread to breeders and layers. In this work, we detected the tetA gene and the corresponding tetR gene (encoding the repressor protein) as well as the truncated transposon Tn1721, which are responsible for the resistance to TET. The presence of the non conjugative transposons from the conjugative plasmid has facilitated the spread of resistance to TET in Salmonella. It was concluded that higher biosecurity practice in poultry farming presents the best option to eliminate infections caused by Salmonella spp. from poultry flocks in Serbia. A rational use of antimicrobials is necessary to prevent any further spread of Salmonella Infantis resistant clones.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia",
volume = "65",
number = "4",
pages = "548-556",
doi = "10.1515/acve-2015-0046"
}
Todorović, D., Velhner, M., Milanov, D., Vidanović, D., Suvajdzić, L., Stojanov, I.,& Krnjaić, D.. (2015). Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 548-556.
https://doi.org/10.1515/acve-2015-0046
Todorović D, Velhner M, Milanov D, Vidanović D, Suvajdzić L, Stojanov I, Krnjaić D. Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):548-556.
doi:10.1515/acve-2015-0046 .
Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Vidanović, Dejan, Suvajdzić, Ljiljana, Stojanov, Igor, Krnjaić, Dejan, "Characterization of tetracycline resistance of salmonella enterica subspecies enterica serovar infantis isolated from poultry in the northern part of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):548-556,
https://doi.org/10.1515/acve-2015-0046 . .
6
5
7

The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region

Pajić, Marko; Todorović, Dalibor; Velhner, Maja; Milanov, Dubravka; Polaček, Vladimir; Đurić, Spomenka; Stojanov, Igor

(Naučni institut za veterinarstvo "Novi Sad", Novi Sad, 2015)

TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Velhner, Maja
AU  - Milanov, Dubravka
AU  - Polaček, Vladimir
AU  - Đurić, Spomenka
AU  - Stojanov, Igor
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1238
AB  - Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. .
AB  - Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region
T1  - Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu
VL  - 8
IS  - 1
SP  - 67
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1238
ER  - 
@article{
author = "Pajić, Marko and Todorović, Dalibor and Velhner, Maja and Milanov, Dubravka and Polaček, Vladimir and Đurić, Spomenka and Stojanov, Igor",
year = "2015",
abstract = "Salmonella causes local and systemic infections of poultry, which may lead to substantial direct and indirect economic losses, presenting also significant risk to human health. The aim of this study was to monitor the occurrence of certain serotypes of Salmonella spp. isolated on poultry farms in Southern Bačka and Srem regions in the period from 2010 to 2014, as recommended by the Book of rules of early detection, diagnostic, prevention of spreading and eradication of Salmonella spp. We analyzed the results obtained from the laboratory for clinical bacteriology to determine number of salmonella cases. From all samples that have been submitted for bacteriology analysis, salmonellas were isolated from 7.3% samples. Salmonella infantis was isolated from 50.3% of all salmonella-positive samples, mostly from materials supplied from broiler farms. Salmonella enteritidis was most frequently isolated in broiler chickens at the rate of 48.2%. There is an increasing trend in the occurrence of Salmonella enteritidis and Salmonella infantis on poultry farms from year to year. Our research revealed the highest incidence of salmonella isolates in broilers that died during transportation or within the first three days upon arrival of chickens. During the five-year research period, 65 samples from parent flocks (63 from broiler breeders and 2 from parent flocks of layers) were salmonella positive, which makes 8.03% of all positive isolates. It is most likely that salmonella infection occurs due to infection of parent flocks and young chickens are infected trough both vertical and horizontal transmission. ., Salmonele kod živine izazivaju infekcije lokalnog i sistemskog karaktera, dovodeći do značajnih kako direktnih tako i indirektnih ekonomskih gubitaka u industrijskom živinarstvu i predstavljaju opasnost po zdravlje ljudi. Cilj ovog rada bio je monitoring određenih sojeva Salmonella spp. propisanih Pravilnikom o utvrđivanju mera za rano otkrivanje, dijagnostiku, sprečavanje širenja, suzbijanje i iskorenjivanje infekcija živine određenim serotipovima salmonella, na teritoriji Sremskog i Južnobačkog okruga u periodu od 2010. do 2014. godine. Analizirali smo rezultate ispitivanja laboratorije za kliničku bakteriologiju. Od ukupno ispitanih bakterijskih infekcija iz kliničkog materijala u ovom petogodišnjem periodu, salmonele su izolovane u 7,3% slučajeva. Ustanovljeno je da je Salmonella infantis izolovana u 50,3% svih pozitivnih uzoraka, najčešće iz materijala dostavljenih sa farmi brojlerskih pilića. Salmonella enteritidis izolovana je u 48,2%, takođe najčešće kod brojlerskih pilića. Prisutan je trend porasta izolata Salmonella enteritidis i Salmonella infantis iz godine u godinu. Našim istraživanjem ustanovili smo da je najviše pozitivnih uzoraka bilo kod brojlera, u transportnim uginućima i uginućima iz prva tri dana starosti. Kod roditeljskih jata bilo je pozitivno 65 uzoraka u ovom petogodišnjem periodu (63 kod teške linije i 2 kod lake linije), što iznosi 8,03 % od svih pozitivnih izolata. Pretpostavlja se da je do infekcije salmonelama moglo doći usled horizontalne i vertikalne infekcije preko roditeljskih jata. .",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region, Epizootiološki značaj Salmonella spp. izolovanih u različitim vidovima živinarske proizvodnje u Južnobačkom i Sremskom okrugu",
volume = "8",
number = "1",
pages = "67-76",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1238"
}
Pajić, M., Todorović, D., Velhner, M., Milanov, D., Polaček, V., Đurić, S.,& Stojanov, I.. (2015). The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 8(1), 67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238
Pajić M, Todorović D, Velhner M, Milanov D, Polaček V, Đurić S, Stojanov I. The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region. in Arhiv veterinarske medicine. 2015;8(1):67-76.
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .
Pajić, Marko, Todorović, Dalibor, Velhner, Maja, Milanov, Dubravka, Polaček, Vladimir, Đurić, Spomenka, Stojanov, Igor, "The epizootiological importance of Salmonella spp. isolated in various aspects of poultry production in the Southern Bačka and Srem region" in Arhiv veterinarske medicine, 8, no. 1 (2015):67-76,
https://hdl.handle.net/21.15107/rcub_veterinar_1238 .

Control of Salmonella in primary production of broiler chickens

Pajić, Marko; Karabasil, Nedjeljko; Todorović, Dalibor; Milanov, Dubravka; Dmitrić, Marko; Lakićević, Brankica; Đorđević, Vesna

(Institut za higijenu i tehnologiju mesa, Beograd, 2015)

TY  - JOUR
AU  - Pajić, Marko
AU  - Karabasil, Nedjeljko
AU  - Todorović, Dalibor
AU  - Milanov, Dubravka
AU  - Dmitrić, Marko
AU  - Lakićević, Brankica
AU  - Đorđević, Vesna
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1245
AB  - Salmonellae represent one of the main causes of zoonotic diseases in people, triggered by ingestion of contaminated food, mostly poultry meat and eggs. The presence of Salmonella in broiler chickens is monitored and controlled according stipulated veterinary-sanitary measures as well as compulsory pre-slaughter control of chicken faeces. The objective of this investigation was to determine the prevalence of Salmonella in primary broiler production and thus to recommend preventive measures for its suppression. The investigation included 37 farms, divided into three categories. Faeces, liver and transport diapers samples were tested in laboratory for clinical bacteriology. During the two-year investigation period (2013-2014), Salmonella was isolated from 13.36% and 14.2% of the samples. Most of the isolates originated from faeces. The most frequent were serovarieties Enteritidis and Infantis. During the period of investigation, the prevalence of salmonellosis in poultry showed the trend of increasing, and moreover, the isolation incidence of certain serovarieties was different.
AB  - Salmonele su jedan od glavnih uzročnika zoonotskih oboljenja ljudi prouzrokovanih konzumacijom kontaminirane hrane, a najčešće mesa živine i jaja. Prisustvo salmonela kod brojlerskih pilića kontroliše se propisanim veterinarsko-sanitarnim merama i obaveznom kontrolom fecesa pilića pred klanje. Cilj ovog istraživanja bio je da se utvrdi prevalencija Salmonella u primarnoj brojlerskoj proizvodnji i da se preporuče preventivne mere za njeno suzbijanje. U istraživanje je bilo uključeno 37 farmi, podeljenih u tri kategorije. Uzorci fecesa, jetri i transportnih pelena su ispitivani u laboratoriji za kliničku bakteriologiju. Tokom dvogodišnjeg perioda ispitivanja (2013-2014), salmonele su izolovane iz 13,36% i 14,2% uzorka. Većina izolata bila je poreklom iz fecesa. Najfrekventnije su ustanovljavani serovarijeteti Enteritidis i Infantis. U navedenom periodu ispitivanja, prevalencija salmoneloze kod živine pokazala je trend povećanja, a određeni serovarijeteti su ustanovljeni učestalije u odnosu na broj ustanovljen prethodnih godina.
PB  - Institut za higijenu i tehnologiju mesa, Beograd
T2  - Tehnologija mesa
T1  - Control of Salmonella in primary production of broiler chickens
T1  - Kontrola Salmonella u primarnoj proizvodnji brojlerskih pilića
VL  - 56
IS  - 2
SP  - 103
EP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1245
ER  - 
@article{
author = "Pajić, Marko and Karabasil, Nedjeljko and Todorović, Dalibor and Milanov, Dubravka and Dmitrić, Marko and Lakićević, Brankica and Đorđević, Vesna",
year = "2015",
abstract = "Salmonellae represent one of the main causes of zoonotic diseases in people, triggered by ingestion of contaminated food, mostly poultry meat and eggs. The presence of Salmonella in broiler chickens is monitored and controlled according stipulated veterinary-sanitary measures as well as compulsory pre-slaughter control of chicken faeces. The objective of this investigation was to determine the prevalence of Salmonella in primary broiler production and thus to recommend preventive measures for its suppression. The investigation included 37 farms, divided into three categories. Faeces, liver and transport diapers samples were tested in laboratory for clinical bacteriology. During the two-year investigation period (2013-2014), Salmonella was isolated from 13.36% and 14.2% of the samples. Most of the isolates originated from faeces. The most frequent were serovarieties Enteritidis and Infantis. During the period of investigation, the prevalence of salmonellosis in poultry showed the trend of increasing, and moreover, the isolation incidence of certain serovarieties was different., Salmonele su jedan od glavnih uzročnika zoonotskih oboljenja ljudi prouzrokovanih konzumacijom kontaminirane hrane, a najčešće mesa živine i jaja. Prisustvo salmonela kod brojlerskih pilića kontroliše se propisanim veterinarsko-sanitarnim merama i obaveznom kontrolom fecesa pilića pred klanje. Cilj ovog istraživanja bio je da se utvrdi prevalencija Salmonella u primarnoj brojlerskoj proizvodnji i da se preporuče preventivne mere za njeno suzbijanje. U istraživanje je bilo uključeno 37 farmi, podeljenih u tri kategorije. Uzorci fecesa, jetri i transportnih pelena su ispitivani u laboratoriji za kliničku bakteriologiju. Tokom dvogodišnjeg perioda ispitivanja (2013-2014), salmonele su izolovane iz 13,36% i 14,2% uzorka. Većina izolata bila je poreklom iz fecesa. Najfrekventnije su ustanovljavani serovarijeteti Enteritidis i Infantis. U navedenom periodu ispitivanja, prevalencija salmoneloze kod živine pokazala je trend povećanja, a određeni serovarijeteti su ustanovljeni učestalije u odnosu na broj ustanovljen prethodnih godina.",
publisher = "Institut za higijenu i tehnologiju mesa, Beograd",
journal = "Tehnologija mesa",
title = "Control of Salmonella in primary production of broiler chickens, Kontrola Salmonella u primarnoj proizvodnji brojlerskih pilića",
volume = "56",
number = "2",
pages = "103-108",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1245"
}
Pajić, M., Karabasil, N., Todorović, D., Milanov, D., Dmitrić, M., Lakićević, B.,& Đorđević, V.. (2015). Control of Salmonella in primary production of broiler chickens. in Tehnologija mesa
Institut za higijenu i tehnologiju mesa, Beograd., 56(2), 103-108.
https://hdl.handle.net/21.15107/rcub_veterinar_1245
Pajić M, Karabasil N, Todorović D, Milanov D, Dmitrić M, Lakićević B, Đorđević V. Control of Salmonella in primary production of broiler chickens. in Tehnologija mesa. 2015;56(2):103-108.
https://hdl.handle.net/21.15107/rcub_veterinar_1245 .
Pajić, Marko, Karabasil, Nedjeljko, Todorović, Dalibor, Milanov, Dubravka, Dmitrić, Marko, Lakićević, Brankica, Đorđević, Vesna, "Control of Salmonella in primary production of broiler chickens" in Tehnologija mesa, 56, no. 2 (2015):103-108,
https://hdl.handle.net/21.15107/rcub_veterinar_1245 .