TY  - CONF
AU  - Milivojević, Marija
AU  - Radovanović, Željko
AU  - Dimitrijević, Suzana
AU  - Petrović, Rada
AU  - Marković, Danica
AU  - Janaćković, Đorđe
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2682
AB  - Tissue engineering strategies for a treatment of large bone defects, which is currently implantation of
bone grafts, are based on the development of biomaterials that mimic the bone tissue and improve
regeneration. 3D printed macroporous titanium-based scaffolds, designed specifically for large bone
defect implantation, provide both good mechanical support and architecture of pores suitable for
ingrowth of bone tissue and better osseointegration. However, the bioinert surface of Ti6Al4V alloy
is not the finest substrate for the growth of cells and mineral matrix deposition.
The aim of this study was to modify the surface of the macroporous Ti scaffold, by coating it with
multiple layers of bioceramics: silicate bioactive glasses (BAG) and Ag-doped calcium
hydroxyapatite (AgHAP), in order to provide bioactivity, biocompatibility, and antibacterial
properties. A dip-coating technique was optimized to provide a thin coating that is homogenous and
uniform inside the porous structure of titanium samples, followed by its melting at optimized
temperature to acquire a continuous BAG layer covered with AgHAP particles.
The characterization of the synthesized bioceramic powders and surface morphology of specimen
were conducted using scanning electron microscopy (SEM) and energy-dispersive X-ray
spectroscopy. The bioactivity was evaluated as the growth of hydroxyapatite crystals on the surface
of the samples after 14 days in Simulated body fluid (SBF), which was determined using SEM.
Biocompatibility of the obtained coatings and bare Ti samples was analyzed in vitro on the L929 cell
line, applying the MTT test. Finally, the antibacterial properties of coated scaffolds were evaluated on
E.coli, S. aureus, and C. albicans.
Evaluation of the morphology of the coatings showed that the homogenous deposition was achieved
by a dip-coating method. Improved bioactivity, biocompatibility, and antimicrobial properties of the
coated scaffold showed the great potential of this approach for future clinical application.
PB  - Belgrade : Materials Research Society of Serbia, 2021
C3  - Twenty-second Annual Conference Yucomat 2021, Herceg Novi, August 30 - September 3, 2021
T1  - Improvement of bioactivity, biocompatibility, and antibacterial properties of titanium scaffold by coating with bioactive glasses and  Ag - doped HAP
SP  - 123
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2682
ER  - 

@conference{
author = "Milivojević, Marija and Radovanović, Željko and Dimitrijević, Suzana and Petrović, Rada and Marković, Danica and Janaćković, Đorđe",
year = "2021",
abstract = "Tissue engineering strategies for a treatment of large bone defects, which is currently implantation of
bone grafts, are based on the development of biomaterials that mimic the bone tissue and improve
regeneration. 3D printed macroporous titanium-based scaffolds, designed specifically for large bone
defect implantation, provide both good mechanical support and architecture of pores suitable for
ingrowth of bone tissue and better osseointegration. However, the bioinert surface of Ti6Al4V alloy
is not the finest substrate for the growth of cells and mineral matrix deposition.
The aim of this study was to modify the surface of the macroporous Ti scaffold, by coating it with
multiple layers of bioceramics: silicate bioactive glasses (BAG) and Ag-doped calcium
hydroxyapatite (AgHAP), in order to provide bioactivity, biocompatibility, and antibacterial
properties. A dip-coating technique was optimized to provide a thin coating that is homogenous and
uniform inside the porous structure of titanium samples, followed by its melting at optimized
temperature to acquire a continuous BAG layer covered with AgHAP particles.
The characterization of the synthesized bioceramic powders and surface morphology of specimen
were conducted using scanning electron microscopy (SEM) and energy-dispersive X-ray
spectroscopy. The bioactivity was evaluated as the growth of hydroxyapatite crystals on the surface
of the samples after 14 days in Simulated body fluid (SBF), which was determined using SEM.
Biocompatibility of the obtained coatings and bare Ti samples was analyzed in vitro on the L929 cell
line, applying the MTT test. Finally, the antibacterial properties of coated scaffolds were evaluated on
E.coli, S. aureus, and C. albicans.
Evaluation of the morphology of the coatings showed that the homogenous deposition was achieved
by a dip-coating method. Improved bioactivity, biocompatibility, and antimicrobial properties of the
coated scaffold showed the great potential of this approach for future clinical application.",
publisher = "Belgrade : Materials Research Society of Serbia, 2021",
journal = "Twenty-second Annual Conference Yucomat 2021, Herceg Novi, August 30 - September 3, 2021",
title = "Improvement of bioactivity, biocompatibility, and antibacterial properties of titanium scaffold by coating with bioactive glasses and  Ag - doped HAP",
pages = "123-123",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2682"
}

Milivojević, M., Radovanović, Ž., Dimitrijević, S., Petrović, R., Marković, D.,& Janaćković, Đ.. (2021). Improvement of bioactivity, biocompatibility, and antibacterial properties of titanium scaffold by coating with bioactive glasses and  Ag - doped HAP. in Twenty-second Annual Conference Yucomat 2021, Herceg Novi, August 30 - September 3, 2021
Belgrade : Materials Research Society of Serbia, 2021., 123-123.
https://hdl.handle.net/21.15107/rcub_veterinar_2682

Milivojević M, Radovanović Ž, Dimitrijević S, Petrović R, Marković D, Janaćković Đ. Improvement of bioactivity, biocompatibility, and antibacterial properties of titanium scaffold by coating with bioactive glasses and  Ag - doped HAP. in Twenty-second Annual Conference Yucomat 2021, Herceg Novi, August 30 - September 3, 2021. 2021;:123-123.
https://hdl.handle.net/21.15107/rcub_veterinar_2682 .

Milivojević, Marija, Radovanović, Željko, Dimitrijević, Suzana, Petrović, Rada, Marković, Danica, Janaćković, Đorđe, "Improvement of bioactivity, biocompatibility, and antibacterial properties of titanium scaffold by coating with bioactive glasses and  Ag - doped HAP" in Twenty-second Annual Conference Yucomat 2021, Herceg Novi, August 30 - September 3, 2021 (2021):123-123,
https://hdl.handle.net/21.15107/rcub_veterinar_2682 .

TY  - JOUR
AU  - Ivanović, Jasna
AU  - Dimitrijević-Branković, Suzana
AU  - Mišić, Dušan
AU  - Ristić, Mihailo
AU  - Žižović, Irena
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1019
AB  - For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.
PB  - Elsevier, Amsterdam
T2  - Journal of Functional Foods
T1  - Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds
VL  - 5
IS  - 1
SP  - 416
EP  - 423
DO  - 10.1016/j.jff.2012.11.014
ER  - 

@article{
author = "Ivanović, Jasna and Dimitrijević-Branković, Suzana and Mišić, Dušan and Ristić, Mihailo and Žižović, Irena",
year = "2013",
abstract = "For the first time, extracts from clove buds obtained by supercritical carbon dioxide extraction were screened for antioxidant and antibacterial activities. Additionally, antioxidant and antibacterial activities of extracts obtained by the supercritical extraction of the clove bud-oregano leaf mixtures were studied. Supercritical extract of pure clove had the highest eugenol (64%) and total phenolic content (530.56 mg GAE/g(extract)). All extracts had antioxidant activity comparable to synthetic antioxidants against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and formation of peroxides. Presence of 0.6% and 5% of oregano extract in the clove extracts obtained from the clove-oregano plant mixtures improved their antioxidant activity with respect to the extract from pure clove. Clove extract showed moderate antibacterial activities against selected Staphylococcus and Enterococcus bacterial strains. Presence of 50% of the oregano extract improved antibacterial activity of clove extract against all tested strains and resulted in a synergistic antibacterial activity against Methicillin-resistant Staphylococcus haemolyticus strain (MIC <= 1.25 mu g/mL). Study demonstrated great potential of supercritical clove extract as natural functional ingredient and the possibility of increasing its antioxidant and antibacterial efficiencies in order to apply lower concentrations and to reduce undesirable flavour notes and toxicological effects in final products.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Functional Foods",
title = "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds",
volume = "5",
number = "1",
pages = "416-423",
doi = "10.1016/j.jff.2012.11.014"
}

Ivanović, J., Dimitrijević-Branković, S., Mišić, D., Ristić, M.,& Žižović, I.. (2013). Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods
Elsevier, Amsterdam., 5(1), 416-423.
https://doi.org/10.1016/j.jff.2012.11.014

Ivanović J, Dimitrijević-Branković S, Mišić D, Ristić M, Žižović I. Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds. in Journal of Functional Foods. 2013;5(1):416-423.
doi:10.1016/j.jff.2012.11.014 .

Ivanović, Jasna, Dimitrijević-Branković, Suzana, Mišić, Dušan, Ristić, Mihailo, Žižović, Irena, "Evaluation and improvement of antioxidant and antibacterial activities of supercritical extracts from clove buds" in Journal of Functional Foods, 5, no. 1 (2013):416-423,
https://doi.org/10.1016/j.jff.2012.11.014 . .

TY  - JOUR
AU  - Stanić, Vojislav
AU  - Janacković, Đorđe
AU  - Dimitrijević, Suzana
AU  - Tanasković, Sladjana B.
AU  - Mitrić, Miodrag
AU  - Pavlović, Mirjana S.
AU  - Krstić, Aleksandra
AU  - Jovanović, Dragoljub
AU  - Raicević, Slavica
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/793
AB  - Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 <= x <= 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright
PB  - Elsevier, Amsterdam
T2  - Applied Surface Science
T1  - Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering
VL  - 257
IS  - 9
SP  - 4510
EP  - 4518
DO  - 10.1016/j.apsusc.2010.12.113
ER  - 

@article{
author = "Stanić, Vojislav and Janacković, Đorđe and Dimitrijević, Suzana and Tanasković, Sladjana B. and Mitrić, Miodrag and Pavlović, Mirjana S. and Krstić, Aleksandra and Jovanović, Dragoljub and Raicević, Slavica",
year = "2011",
abstract = "Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002 <= x <= 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright",
publisher = "Elsevier, Amsterdam",
journal = "Applied Surface Science",
title = "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering",
volume = "257",
number = "9",
pages = "4510-4518",
doi = "10.1016/j.apsusc.2010.12.113"
}

Stanić, V., Janacković, Đ., Dimitrijević, S., Tanasković, S. B., Mitrić, M., Pavlović, M. S., Krstić, A., Jovanović, D.,& Raicević, S.. (2011). Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science
Elsevier, Amsterdam., 257(9), 4510-4518.
https://doi.org/10.1016/j.apsusc.2010.12.113

Stanić V, Janacković Đ, Dimitrijević S, Tanasković SB, Mitrić M, Pavlović MS, Krstić A, Jovanović D, Raicević S. Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science. 2011;257(9):4510-4518.
doi:10.1016/j.apsusc.2010.12.113 .

Stanić, Vojislav, Janacković, Đorđe, Dimitrijević, Suzana, Tanasković, Sladjana B., Mitrić, Miodrag, Pavlović, Mirjana S., Krstić, Aleksandra, Jovanović, Dragoljub, Raicević, Slavica, "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering" in Applied Surface Science, 257, no. 9 (2011):4510-4518,
https://doi.org/10.1016/j.apsusc.2010.12.113 . .