@article{
author = "Tešović, Bojana and Nišavić, Jakov and Banović-Đeri, Bojana and Petrović, Tamaš and Radalj, Andrea and Šekler, Milanko and Matović, Kazimir and Debeljak, Zoran and Vasković, Nikola and Dmitrić, Marko and Vidanović, Dejan",
year = "2023",
abstract = "West Nile virus (WNV) can affect humans, birds, horses and another mammals, causing asymptomatic infection, mild febrile disease, neurological and systematic disease and death. In order to gain insight into the
prevalence of WNV, a monitoring program has been established in the Republic of Serbia. Whole genome
sequencing is essential for the molecular epizootiological analysis of virus entry and transmission routes,
especially in high-risk regions. This paper describes the development of a multiplex PCR based NGS protocol
for whole genome sequencing of WNV lineage 2 directly from biological samples using Oxford Nanopore
(ONT) platform. The results obtained using this platform, confirmed by Sanger sequencing, indicate that this
protocol can be applied to obtain whole sequences of the WNV genome, even when the virus concentration
in the sample is medium, Ct value is approximately 30. The use of this protocol does not require prior virus
isolation on cell culture nor the depletion of host nucleic acids.",
publisher = "Elsevier",
journal = "Diagnostic Microbiology and Infectious Disease",
title = "Development of multiplex PCR based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological samples using Oxford Nanopore platform",
volume = "105",
number = "2",
pages = "115852",
doi = "10.1016/j.diagmicrobio.2022.115852"
}