TY  - JOUR
AU  - Danilović-Luković, Jelena
AU  - Korac, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Puskas, Nela
AU  - Kovačević-Filipović, Milica
AU  - Radovanović, Anita
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1428
AB  - Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Micron
T1  - Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach
VL  - 90
SP  - 33
EP  - 42
DO  - 10.1016/j.micron.2016.08.007
ER  - 

@article{
author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Puskas, Nela and Kovačević-Filipović, Milica and Radovanović, Anita",
year = "2016",
abstract = "Thyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian follicles in female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentration of TH on primordial follicles pool formation during neonatal and early infantile period of rat pups was not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negative influence on primordial follicles pool formation and population of resting oocytes in female rat offspring. The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female rat pups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively. Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM). Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicles were determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin red staining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum (sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclear antigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of follicles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes and nests, an increased number of primordial follicles and a decreased number of primary and secondary follicles, while in early infantile period, increased number of primary, secondary and preantral follicles were found. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content of dilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups was increased. Apoptosis markers have not shown significant difference between groups but PCNA had an increased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Light and electron microscopy analysis indicate that previously detected premature ovarian follicular atresia in pre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial follicles followed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Micron",
title = "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach",
volume = "90",
pages = "33-42",
doi = "10.1016/j.micron.2016.08.007"
}

Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Puskas, N., Kovačević-Filipović, M.,& Radovanović, A.. (2016). Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron
Pergamon-Elsevier Science Ltd, Oxford., 90, 33-42.
https://doi.org/10.1016/j.micron.2016.08.007

Danilović-Luković J, Korac A, Milošević I, Lužajić T, Puskas N, Kovačević-Filipović M, Radovanović A. Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron. 2016;90:33-42.
doi:10.1016/j.micron.2016.08.007 .

Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Puskas, Nela, Kovačević-Filipović, Milica, Radovanović, Anita, "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach" in Micron, 90 (2016):33-42,
https://doi.org/10.1016/j.micron.2016.08.007 . .

TY  - JOUR
AU  - Prokić, Bogomir Bolka
AU  - Bačić, G.
AU  - Prokić, B.
AU  - Kalijadis, Ana
AU  - Todorović, Vera
AU  - Puškaš, Nela
AU  - Vidojević, D.
AU  - Laušević, Mila
AU  - Laušević, Z.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/914
AB  - In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials.
AB  - U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues
T1  - In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom
VL  - 62
IS  - 5-6
SP  - 683
EP  - 696
DO  - 10.2298/AVB1206683P
ER  - 

@article{
author = "Prokić, Bogomir Bolka and Bačić, G. and Prokić, B. and Kalijadis, Ana and Todorović, Vera and Puškaš, Nela and Vidojević, D. and Laušević, Mila and Laušević, Z.",
year = "2012",
abstract = "In modern medicine implants are very important and so is their design and choice of materials. Almost equally important is the choice of imaging technique used to in vivo monitor their fate and biocompatibility. The aim of this study was to evaluate the ability of magnetic resonance imaging (MRI) in monitoring the biocompatibility of two newly designed carbon fibers. We have analyzed the interaction of surface functionalized carbon fibers (basic and acidic) with muscle and subcutaneous tissues of rabbits. MRI techniques showed to be useful in longitudinal monitoring of the surrounding tissues, assessment of biocompatibility of new implants, and in the distinction of in vivo surgical edema from inflammation. Histopathology confirmed MRI results, thus showing that MRI has a great potential for in vivo studies of such materials., U modernoj medicine implanti su veoma važni, kao i njihovo dizajniranje i izbor materijala. Isto tako je značajan i izbor tehnike kojom se prati in vivo njihova sudbina i biokompatibilnost. Cilj ove studije je bila procena mogućnosti praćenja biokompatibilnosti dva novo dizajnirana karbonska vlakna pomoću magnetic resonance imaging (MRI). Analizirali smo interakciju karbonskih vlakana sa funkcionalizovanom površinom (kiselinom i bazom) sa mišićnim i potkožnim tkivom kunića. MRI tehnika se pokazala uspešnom u toku praćenja reakcije okolnog tkiva, i procene biokompatibilnosti nove vrste implanata, jer je uspela da in vivo odvoji hirurški edem od inflamacije. Patohistološka analiza je potvrdila rezultate MRI pokazujući time da MRI ima veliki potencijal za in vivo studije takvih materijala.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues, In vivo MRI procena biokompatibilnosti funkcionalizovanih karbonskih vlakana u reakciji sa mekim tkivom",
volume = "62",
number = "5-6",
pages = "683-696",
doi = "10.2298/AVB1206683P"
}

Prokić, B. B., Bačić, G., Prokić, B., Kalijadis, A., Todorović, V., Puškaš, N., Vidojević, D., Laušević, M.,& Laušević, Z.. (2012). In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 683-696.
https://doi.org/10.2298/AVB1206683P

Prokić BB, Bačić G, Prokić B, Kalijadis A, Todorović V, Puškaš N, Vidojević D, Laušević M, Laušević Z. In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues. in Acta Veterinaria-Beograd. 2012;62(5-6):683-696.
doi:10.2298/AVB1206683P .

Prokić, Bogomir Bolka, Bačić, G., Prokić, B., Kalijadis, Ana, Todorović, Vera, Puškaš, Nela, Vidojević, D., Laušević, Mila, Laušević, Z., "In vivo MRI biocompatibility evaluation of functionalized carbon fibers in reaction with soft tissues" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):683-696,
https://doi.org/10.2298/AVB1206683P . .