TY  - JOUR
AU  - Pajić, Marko
AU  - Todorović, Dalibor
AU  - Knežević, Slobodan
AU  - Prunić, Bojana
AU  - Velhner, Maja
AU  - Ostojić Andrić, Dušica
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2902
AB  - Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.
PB  - MDPI
T2  - Life
T1  - Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia
VL  - 13
IS  - 4
SP  - 1
EP  - 13
DO  - 10.3390/life13041039
ER  - 

@article{
author = "Pajić, Marko and Todorović, Dalibor and Knežević, Slobodan and Prunić, Bojana and Velhner, Maja and Ostojić Andrić, Dušica and Stanimirović, Zoran",
year = "2023",
abstract = "Coccidiosis is a significant poultry disease caused by the Eimeria species. This study aims to
determine the prevalence of Eimeria spp. on broiler farms in Vojvodina, along with the identification
of parasite species, and assess the implemented biosecurity measures. The study was conducted on
100 broiler chicken farms (28 small-sized; 34 medium-sized; 38 large-sized farms) from June 2018 to
December 2021. One pooled sample of faeces was collected from three to six-week-old chickens from
each farm, and assessment of biosecurity measures was carried out using a questionnaire. Using the
PCR method, DNA of Eimeria was found in 59 samples (59%), while 41 samples (41%) were negative.
Four species of Eimeria were identified, and their prevalence was the following: E. acervulina (37%),
E. maxima (17%), E. mitis (25%) and E. tenella (48%). A significant difference (p < 0.05) was established
in the number of oocysts in flocks from small-sized farms compared to medium-sized farms. It was
found that regular implementation of disinfection, disinsection and deratisation measures, as well as
all the biosecurity measures, can significantly reduce the occurrence of coccidiosis. These results will
help to develop better strategies for the control and prevention of coccidiosis on farms.",
publisher = "MDPI",
journal = "Life",
title = "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia",
volume = "13",
number = "4",
pages = "1-13",
doi = "10.3390/life13041039"
}

Pajić, M., Todorović, D., Knežević, S., Prunić, B., Velhner, M., Ostojić Andrić, D.,& Stanimirović, Z.. (2023). Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life
MDPI., 13(4), 1-13.
https://doi.org/10.3390/life13041039

Pajić M, Todorović D, Knežević S, Prunić B, Velhner M, Ostojić Andrić D, Stanimirović Z. Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia. in Life. 2023;13(4):1-13.
doi:10.3390/life13041039 .

Pajić, Marko, Todorović, Dalibor, Knežević, Slobodan, Prunić, Bojana, Velhner, Maja, Ostojić Andrić, Dušica, Stanimirović, Zoran, "Molecular Investigation of Eimeria Species in Broiler Farms in the Province of Vojvodina, Serbia" in Life, 13, no. 4 (2023):1-13,
https://doi.org/10.3390/life13041039 . .

TY  - JOUR
PY  - 2022
UR  - https://repo.niv.ns.ac.rs/xmlui/handle/123456789/470
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2462
AB  - This experiment was performed to investigate the protective effect of a new combined adsorbent (bentonite + yeast cellwall extract) previously tested in vitro, on the occurrence of pathological lesions in broilers fed a diet contaminated with aflatoxinB1 until 21 days of age. A total of 96 one-day old Ross 308 hybrid broiler chickens were divided into four groups: group I (control),group II (5 g/kg adsorbent), group III (5 g/kg adsorbent + 2 mg/kg aflatoxin B1), and group IV (2 mg/kg aflatoxin B1). Grossly,AFB1 and AFB1+adsorbent fed birds showed enlargement, friable texture of liver and enlargement and pallorness of kidneys. Spleenrevealed size reduction. No gross-pathological changes were observed in control and group II. Histopathologically, lesions wereobserved in aflatoxicated groups. Liver revealed vacuolar cell degeneration, periportal and perivascular infiltration of mononuclearcells. Kidneys revealed mild to moderate degree of haemorrhages, tubular epithelial necrosis and infiltration of mononuclearcells along with heterophils. In group IV, catarrhal enteritis characterized by desquamation of epithelial cells were found in theduodenum. A milder form of gross and histopathological lesions was seen in group III. In conclusion, the present study revealedthat supplementation of a new combined toxin binder product in the concentration of 5 g/kg could not completely ameliorateaflatoxicity in broilers, although it met the stringent European regulation requirements for the minimum of 90% aflatoxin bindingefficiency in in vitro study.
PB  - Kafkas University, Faculty of Veterinary Medicine
T1  - Evaluation of the Effects of New Combined Adsorbent on Occurrence of Pathological Lesions in Aflatoxin B1‐Challenged Broiler Chickens
VL  - 28
IS  - 4
SP  - 185
EP  - 191
DO  - 10.9775/kvfd.2021.26654
ER  - 

@article{
year = "2022",
abstract = "This experiment was performed to investigate the protective effect of a new combined adsorbent (bentonite + yeast cellwall extract) previously tested in vitro, on the occurrence of pathological lesions in broilers fed a diet contaminated with aflatoxinB1 until 21 days of age. A total of 96 one-day old Ross 308 hybrid broiler chickens were divided into four groups: group I (control),group II (5 g/kg adsorbent), group III (5 g/kg adsorbent + 2 mg/kg aflatoxin B1), and group IV (2 mg/kg aflatoxin B1). Grossly,AFB1 and AFB1+adsorbent fed birds showed enlargement, friable texture of liver and enlargement and pallorness of kidneys. Spleenrevealed size reduction. No gross-pathological changes were observed in control and group II. Histopathologically, lesions wereobserved in aflatoxicated groups. Liver revealed vacuolar cell degeneration, periportal and perivascular infiltration of mononuclearcells. Kidneys revealed mild to moderate degree of haemorrhages, tubular epithelial necrosis and infiltration of mononuclearcells along with heterophils. In group IV, catarrhal enteritis characterized by desquamation of epithelial cells were found in theduodenum. A milder form of gross and histopathological lesions was seen in group III. In conclusion, the present study revealedthat supplementation of a new combined toxin binder product in the concentration of 5 g/kg could not completely ameliorateaflatoxicity in broilers, although it met the stringent European regulation requirements for the minimum of 90% aflatoxin bindingefficiency in in vitro study.",
publisher = "Kafkas University, Faculty of Veterinary Medicine",
title = "Evaluation of the Effects of New Combined Adsorbent on Occurrence of Pathological Lesions in Aflatoxin B1‐Challenged Broiler Chickens",
volume = "28",
number = "4",
pages = "185-191",
doi = "10.9775/kvfd.2021.26654"
}

(2022). Evaluation of the Effects of New Combined Adsorbent on Occurrence of Pathological Lesions in Aflatoxin B1‐Challenged Broiler Chickens. 
Kafkas University, Faculty of Veterinary Medicine., 28(4), 185-191.
https://doi.org/10.9775/kvfd.2021.26654

Evaluation of the Effects of New Combined Adsorbent on Occurrence of Pathological Lesions in Aflatoxin B1‐Challenged Broiler Chickens. 2022;28(4):185-191.
doi:10.9775/kvfd.2021.26654 .

"Evaluation of the Effects of New Combined Adsorbent on Occurrence of Pathological Lesions in Aflatoxin B1‐Challenged Broiler Chickens", 28, no. 4 (2022):185-191,
https://doi.org/10.9775/kvfd.2021.26654 . .

TY  - JOUR
AU  - Pajić, Marko
AU  - Aleksić, Nevenka
AU  - Vejnović, Branislav
AU  - Polaček, Vladimir
AU  - Novakov, Nikolina
AU  - Ostojić-Andrić, Dušica
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1733
AB  - The influence of certain anticoccidial drugs on oxidative stress in broiler chickens infected with Eimeria species was assessed. There were two untreated (uninfected and infected), and three groups infected and treated with anticoccidials. The first treated group (Ro) was given robenidine, the 2nd a herbal anticoccidial (Herb) and the 3rd the combination of robenidine and the herbal anticoccidial (Ro+Herb). All infected groups were on day 14 challenged with oral inoculation of oocysts. The activities of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST), and the concentration of malondialdehyde (MDA) were estimated in blood taken on days 21 and 40. The oocyst numbers were calculated per gram, and chicken body weight and feed conversion ratio (FCR) measured. The activities of CAT, GST and the level of MDA were significantly lower (P<0.05), whilst the activity of SOD was higher in infected chickens treated with anticoccidials (P<0.05) in comparison to those untreated. The most prominent change in the parameters of oxidative stress was recorded in the Ro+Herb group. In chickens treated with anticoccidials body weight was significantly higher (P<0.05), and the FCR and the oocyst counts significantly lower (P<0.05) than in untreated chickens. Oocyst counts were lower in the Ro and Ro+Herb groups than in the Herb group. Our study demonstrated that both anticoccidial substances exerted antioxidant and anticoccidial effects.
PB  - Kafkas Univ, Veteriner Fakultesi Dergisi, Kars
T2  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
T1  - Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species
VL  - 25
IS  - 3
SP  - 379
EP  - 385
DO  - 10.9775/kvfd.2018.21021
ER  - 

@article{
author = "Pajić, Marko and Aleksić, Nevenka and Vejnović, Branislav and Polaček, Vladimir and Novakov, Nikolina and Ostojić-Andrić, Dušica and Stanimirović, Zoran",
year = "2019",
abstract = "The influence of certain anticoccidial drugs on oxidative stress in broiler chickens infected with Eimeria species was assessed. There were two untreated (uninfected and infected), and three groups infected and treated with anticoccidials. The first treated group (Ro) was given robenidine, the 2nd a herbal anticoccidial (Herb) and the 3rd the combination of robenidine and the herbal anticoccidial (Ro+Herb). All infected groups were on day 14 challenged with oral inoculation of oocysts. The activities of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST), and the concentration of malondialdehyde (MDA) were estimated in blood taken on days 21 and 40. The oocyst numbers were calculated per gram, and chicken body weight and feed conversion ratio (FCR) measured. The activities of CAT, GST and the level of MDA were significantly lower (P<0.05), whilst the activity of SOD was higher in infected chickens treated with anticoccidials (P<0.05) in comparison to those untreated. The most prominent change in the parameters of oxidative stress was recorded in the Ro+Herb group. In chickens treated with anticoccidials body weight was significantly higher (P<0.05), and the FCR and the oocyst counts significantly lower (P<0.05) than in untreated chickens. Oocyst counts were lower in the Ro and Ro+Herb groups than in the Herb group. Our study demonstrated that both anticoccidial substances exerted antioxidant and anticoccidial effects.",
publisher = "Kafkas Univ, Veteriner Fakultesi Dergisi, Kars",
journal = "Kafkas Üniversitesi Veteriner Fakültesi Dergisi",
title = "Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species",
volume = "25",
number = "3",
pages = "379-385",
doi = "10.9775/kvfd.2018.21021"
}

Pajić, M., Aleksić, N., Vejnović, B., Polaček, V., Novakov, N., Ostojić-Andrić, D.,& Stanimirović, Z.. (2019). Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi
Kafkas Univ, Veteriner Fakultesi Dergisi, Kars., 25(3), 379-385.
https://doi.org/10.9775/kvfd.2018.21021

Pajić M, Aleksić N, Vejnović B, Polaček V, Novakov N, Ostojić-Andrić D, Stanimirović Z. Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species. in Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2019;25(3):379-385.
doi:10.9775/kvfd.2018.21021 .

Pajić, Marko, Aleksić, Nevenka, Vejnović, Branislav, Polaček, Vladimir, Novakov, Nikolina, Ostojić-Andrić, Dušica, Stanimirović, Zoran, "Influence of Anticoccidials on Oxidative Stress, Production Performance and Faecal Oocyst Counts in Broiler Chickens Infected with Eimeria Species" in Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25, no. 3 (2019):379-385,
https://doi.org/10.9775/kvfd.2018.21021 . .

TY  - JOUR
AU  - Dimitrijević, Blagoje
AU  - Jović, Slavoljub
AU  - Ostojić-Andrić, Dušica
AU  - Savić, Mila
AU  - Becskei, Zsolt
AU  - Davidović, Vesna
AU  - Joksimović-Todorović, Mirjana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1321
AB  - The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols.
AB  - Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters
T1  - Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara
VL  - 32
IS  - 4
SP  - 369
EP  - 381
DO  - 10.2298/bah1604369D
ER  - 

@article{
author = "Dimitrijević, Blagoje and Jović, Slavoljub and Ostojić-Andrić, Dušica and Savić, Mila and Becskei, Zsolt and Davidović, Vesna and Joksimović-Todorović, Mirjana",
year = "2016",
abstract = "The aim of this study was to determine and evaluate the basic haematological parameters in conditions of natural infection of sheep with Strongyloides papillosus, as well as after the administration of antihelminthic albendazole (ABZ). Based on the intensity of infection with S. papillosus the sheep were divided into three groups: mild, moderate and high, and after that the sheep received a single dose of ABZ of 5mg/kg per body weight, per orally. Sampling of faeces and blood for parasitological and haematological assaying respectively, was performed on the 0 and the 21st day after the treatment with ABZ. The presence of parasitic infection with S. papillosus leads to a decrease of erythrocyte count, while the lowest values were established in the group with the highest intensity of parasitic infection (p ˂ 0.001). After treatment with ABZ the decrease of erythrocyte count was more prominent, which was, based on comparison with control groups C1 and C2, unequivocally established to be the consequence of treatment with ABZ. Detected values of haematocrit and erythrocyte indices indicated the presence of parasitic infection: the lowest values were established in the group with the highest intensity of parasitic infection. After treatment with ABZ haematocrit levels in control group C2 were statistically significantly lower compared to the control group C1 (p ˂ 0.001). In the presence of parasitic infection, the neutrophil and eosinophil counts increased almost linearly up to the value of 44.24±2.50% and 13.29±0.61% respectively, in the group of sheep with the highest intensity of parasitic infection (p ˂ 0.001; compared to control group C1). After treatment with ABZ the decrease of the number of these white blood cells is statistically significant (p ˂ 0.001). Bearing in mind our previous research and the connection of disbalanced redox equilibrium after the treatment with ABZ with changes, it is necessary to include antioxidative substances in the anti-parasitic treatment protocols., Cilj ovog istraživanja bio je da se utvrde i procene osnovni hematološki parametri u uslovima prirodne infekcije ovaca sa Strongyloides papillosus, kao i nakon primene antihelmintika albendazola (ABZ). Na osnovu intenziteta infekcije sa S. papillosus ovce su podeljene u tri grupe: niski, srednji i visoki intenzitet infekcije, a zatim su ovce jednokratno dobile peroralno ABZ, u terapijskoj dozi od 5 mg/kg telesne mase. Uzorkovanje fecesa za parazitološka i za hematološka ispitivanja obavljeno je nultog i 21. dana od primene ABZ. Utvrđeno je da prisustvo parazitske infekcije sa S. papillosus dovodi do pada broja eritrocita, pri čemu su najniže vrednosti utvrđene u grupi sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ pad broja eritrocita je izraženiji, što je nesumnjivo nastalo kao posledica terapije ABZ (na osnovu poređenja C1 i C2). Utvrđene vrednosti hematokrita i eritrocitnih indeksa su ukazivali na postojanje parazitske infekcije; najniže vrednosti su utvrđene kod grupe sa najvećim intenzitetom parazitske infekcije. Nakon terapije sa ABZ vrednosti hematokrita kod C2 bile su statistički značajno niže u odnosu na kontrolnu grupu C1 (p ˂ 0,001). U prisustvu parazitske infekcije broj neutrofila i eozinofila povećava se gotovo linearno, do vrednosti od 44,24±2,50% kod neutrofila, odnosno od 13,29±0,61% kod eozinofila u grupi ovaca sa najvećim intenzitetom parazitske infekcije (p ˂ 0,001). Nakon terapije sa ABZ broj ovih ćelija bele krvne loze smanjuje se statistički značajno (p ˂ 0,001). Imajući u vidu naša prethodna istraživanja i povezanost narušene redoks ravnoteže posle terapije sa ABZ sa promenama utvrđenim u ovom istraživanju, neophodno je u antiparazitske terapijske protokole uključiti antioksidativne supstance.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters, Infekcija ovaca sa Strongyloides papillosus - uticaj intenziteta parazitske infekcije i terapije albendazolom na vrednosti osnovnih hematoloških parametara",
volume = "32",
number = "4",
pages = "369-381",
doi = "10.2298/bah1604369D"
}

Dimitrijević, B., Jović, S., Ostojić-Andrić, D., Savić, M., Becskei, Z., Davidović, V.,& Joksimović-Todorović, M.. (2016). Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(4), 369-381.
https://doi.org/10.2298/bah1604369D

Dimitrijević B, Jović S, Ostojić-Andrić D, Savić M, Becskei Z, Davidović V, Joksimović-Todorović M. Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters. in Biotechnology in Animal Husbandry. 2016;32(4):369-381.
doi:10.2298/bah1604369D .

Dimitrijević, Blagoje, Jović, Slavoljub, Ostojić-Andrić, Dušica, Savić, Mila, Becskei, Zsolt, Davidović, Vesna, Joksimović-Todorović, Mirjana, "Infection with Strongyloides papillosus in sheep: Effect of parasitic infection and treatment with albendazole on basic haematological parameters" in Biotechnology in Animal Husbandry, 32, no. 4 (2016):369-381,
https://doi.org/10.2298/bah1604369D . .

TY  - JOUR
AU  - Krsmanović, Milorad
AU  - Đoković, Radojica
AU  - Cincović, Marko
AU  - Ostojić-Andrić, Dušica
AU  - Bojkovski, Jovan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1319
AB  - This study examined the activities of aspartate-aminotransferase (AST), gamma-glutamyl-transferase (GGT) and lactate-dehydrogenase (LDH) in the blood serum of 45 dairy Simmental cows divided into three groups according to production period. The first group (n=15) consisted of late pregnant dairy cows, the second group (n=15) cows in the early lactation, and the third group (n=15) cow in mid lactation. The significant higher activity (P lt 0.05) of AST, GGT and LDH were determined in the early lactation period than in dry period and during full lactation. Research results showed possibility of mild degree of hepatic lesions, probably due to fat infiltration in early lactation cows. Serum AST enzyme activities were significant correlated (P lt 0.05) with GGT and LDH activities and may be most sensitive indicator.
AB  - U ovom radu je određivana aktivnost aspartat-aminotransferaze (AST), gama-glutamat-transferaze (GGT) i laktat dehidrogenaze (LDH) u krvnom serumu kod 45 simentalskih mlečnih krava, podeljenih u tri grupe u zavisnosti od produktivnog perioda. Prvu grupu (n=15) su činile visoko gravidne krave, drugu grupu (n=15) krave u ranoj laktaciji, a treću grupu (n=15) krave tokom pune laktacije. Statistički značajno veće aktivnosti AST (P lt 0.05),GGT (P lt 0.05) i LDH (P lt 0.05) u krvnom serumu su utvrđene kod krava u ranoj laktaciji u odnosu na aktivnosti ovih enzima u serumu kod zasušenih krava i krava u punoj laktaciji. Dobijeni rezultati ukazuju na mogućnost blagog stepena oštećenja ćelija jetre, odnosno masnu infiltraciju hepatocita kod krava na početku laktacije. Serumske aktivnosti AST su bile u značajnoj korelaciji (P lt 0.05) sa aktivnostima GGT i LDH u krvnom serumu pa se AST može smatrati pouzdanim indikatorom u tvrđivanju funkcionalnog stanja jetre kod mlečnih krava tokom peripartalnog perioda i pune laktacije.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations
T1  - Određivanje aktivnosti specifičnih enzima krvi u peripartalnom periodu i tokom pune laktacije
VL  - 32
IS  - 1
SP  - 9
EP  - 14
DO  - 10.2298/BAH1601009K
ER  - 

@article{
author = "Krsmanović, Milorad and Đoković, Radojica and Cincović, Marko and Ostojić-Andrić, Dušica and Bojkovski, Jovan",
year = "2016",
abstract = "This study examined the activities of aspartate-aminotransferase (AST), gamma-glutamyl-transferase (GGT) and lactate-dehydrogenase (LDH) in the blood serum of 45 dairy Simmental cows divided into three groups according to production period. The first group (n=15) consisted of late pregnant dairy cows, the second group (n=15) cows in the early lactation, and the third group (n=15) cow in mid lactation. The significant higher activity (P lt 0.05) of AST, GGT and LDH were determined in the early lactation period than in dry period and during full lactation. Research results showed possibility of mild degree of hepatic lesions, probably due to fat infiltration in early lactation cows. Serum AST enzyme activities were significant correlated (P lt 0.05) with GGT and LDH activities and may be most sensitive indicator., U ovom radu je određivana aktivnost aspartat-aminotransferaze (AST), gama-glutamat-transferaze (GGT) i laktat dehidrogenaze (LDH) u krvnom serumu kod 45 simentalskih mlečnih krava, podeljenih u tri grupe u zavisnosti od produktivnog perioda. Prvu grupu (n=15) su činile visoko gravidne krave, drugu grupu (n=15) krave u ranoj laktaciji, a treću grupu (n=15) krave tokom pune laktacije. Statistički značajno veće aktivnosti AST (P lt 0.05),GGT (P lt 0.05) i LDH (P lt 0.05) u krvnom serumu su utvrđene kod krava u ranoj laktaciji u odnosu na aktivnosti ovih enzima u serumu kod zasušenih krava i krava u punoj laktaciji. Dobijeni rezultati ukazuju na mogućnost blagog stepena oštećenja ćelija jetre, odnosno masnu infiltraciju hepatocita kod krava na početku laktacije. Serumske aktivnosti AST su bile u značajnoj korelaciji (P lt 0.05) sa aktivnostima GGT i LDH u krvnom serumu pa se AST može smatrati pouzdanim indikatorom u tvrđivanju funkcionalnog stanja jetre kod mlečnih krava tokom peripartalnog perioda i pune laktacije.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations, Određivanje aktivnosti specifičnih enzima krvi u peripartalnom periodu i tokom pune laktacije",
volume = "32",
number = "1",
pages = "9-14",
doi = "10.2298/BAH1601009K"
}

Krsmanović, M., Đoković, R., Cincović, M., Ostojić-Andrić, D.,& Bojkovski, J.. (2016). Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 32(1), 9-14.
https://doi.org/10.2298/BAH1601009K

Krsmanović M, Đoković R, Cincović M, Ostojić-Andrić D, Bojkovski J. Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations. in Biotechnology in Animal Husbandry. 2016;32(1):9-14.
doi:10.2298/BAH1601009K .

Krsmanović, Milorad, Đoković, Radojica, Cincović, Marko, Ostojić-Andrić, Dušica, Bojkovski, Jovan, "Determination of the activity of specific enzymes of blood in the peripartum period and during the full lactations" in Biotechnology in Animal Husbandry, 32, no. 1 (2016):9-14,
https://doi.org/10.2298/BAH1601009K . .

TY  - JOUR
AU  - Bakr, H.A.
AU  - Hassan, M.S.
AU  - Giadinis, Nektarios
AU  - Panousis, N.
AU  - Ostojić-Andrić, Dušica
AU  - Abd, El-Tawab M.M.
AU  - Bojkovski, Jovan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1255
AB  - Data concerning the effect of probiotics supplementation on many parameters concurrently at the same cows are lacking. Therefore, the objective of this experiment was to investigate the effects of Saccharomyces cerevisiae feeding on rumen, blood and milk parameters together in high- producing dairy cattle during the transition and early lactation period. Sixteen clinically healthy Holstein cows were divided into 2 groups: a control group of 6 cows and a probiotics-fed group of 10 cows. Rumen fluid and blood samples were collected 21 days before the expected calving as well as 7, 15, 30, 45 and 60 days- in-milk (DIM). Milk yield for each animal was recorded every 2 weeks. Individual milk samples were collected 15, 30, 45 and 60 DIM. Ruminal pH and rumen ammonia nitrogen were significantly lower, whereas total volatile fatty acids were significantly higher in yeast-fed animals compared with controls throughout the study. Serum concentrations of total proteins and globulins were higher, while albumins were lower in the yeast-treated group. Serum glucose levels were significantly higher in yeast-supplemented animals. Serum triglycerides, high density lipoproteins, and low density lipoproteins concentrations were lower, with cholesterol being significantly lower in the treated group. Milk production and milk fat percentage were higher, whereas milk protein percentage and somatic cell count were decreased in yeast-supplemented cows throughout the study. These results suggest that supplementation of S. cerevisiae to dairy cows rations during transition and early lactation period improve their health and milk production parameters.
AB  - Podaci koji bi se odnosili na uticaj suplementacije probiotika na više parametara istovremeno na istim grlima nedostaju u literaturi. Stoga je cilj ovog eksperimenta bio da se ispita uticaj Saccharomices cerevisiae u obroku/hrani na parametre rumena, krvi i mleka zajedno u visoko-proizvodnim mlečnim govedima tokom tranzicije i početkom laktacije. Šesnaest klinički zdravih holštajn krava je podeljeno u 2 grupe: kontrolna grupa od 6 krava i grupa od 10 krava hranjenih probiotikom u obroku. Buražna tečnost i uzorci krvi su sakupljeni 21 dan pre očekivanog teljenja, kao i 7, 15, 30, 45 i 60 dana tokom laktacije (days in milk - DIM). Prinos mleka za svaku životinju zabeležen je svake 2 nedelje. Pojedinačni uzorci mleka su prikupljeni 15, 30, 45 i 60 DIM. pH buraga i buražni amonijačni azot su bili značajno niži, dok su ukupne isparljive masne kiseline bile značajno veće kod životinja hranjenih kvascem u poređenju sa kontrolama kroz celu studiju. Serumske koncentracije ukupnih proteina i globulina bile su više, dok su koncentracije albumina bile niže u grupi sa kvascem. Serumski nivoi glukoze bili su značajno viši kod životinja sa dodatkom kvasca. Trigliceridi u serumu, koncentracije lipoproteina visoke i niske gustine bile su niže, sa holesterolom koji je bio znatno niži u tretiranoj grupi. Proizvodnja mleka i procent mlečne masti bili su viši, dok je sadržaj proteina mleka i somatskih ćelija bio niži u krava hranjenih sa dodatkom kvasca kroz celu studiju. Ovi rezultati ukazuju na to da dodatak S. cerevisiae u obrocima muznih krava tokom tranzicije i rane laktacije poboljšava njihovo zdravlje i parametre proizvodnje mleka.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Effect of Saccharomyces cerevisiae supplementation on health and performance of dairy cows during transition and early lactation period
T1  - Uticaj dodatka Saccharomyces cerevisiae u ishrani na zdravlje i proizvodnju krava tokom perioda tranzicije i početka laktacije
VL  - 31
IS  - 3
SP  - 349
EP  - 364
DO  - 10.2298/BAH1503349B
ER  - 

@article{
author = "Bakr, H.A. and Hassan, M.S. and Giadinis, Nektarios and Panousis, N. and Ostojić-Andrić, Dušica and Abd, El-Tawab M.M. and Bojkovski, Jovan",
year = "2015",
abstract = "Data concerning the effect of probiotics supplementation on many parameters concurrently at the same cows are lacking. Therefore, the objective of this experiment was to investigate the effects of Saccharomyces cerevisiae feeding on rumen, blood and milk parameters together in high- producing dairy cattle during the transition and early lactation period. Sixteen clinically healthy Holstein cows were divided into 2 groups: a control group of 6 cows and a probiotics-fed group of 10 cows. Rumen fluid and blood samples were collected 21 days before the expected calving as well as 7, 15, 30, 45 and 60 days- in-milk (DIM). Milk yield for each animal was recorded every 2 weeks. Individual milk samples were collected 15, 30, 45 and 60 DIM. Ruminal pH and rumen ammonia nitrogen were significantly lower, whereas total volatile fatty acids were significantly higher in yeast-fed animals compared with controls throughout the study. Serum concentrations of total proteins and globulins were higher, while albumins were lower in the yeast-treated group. Serum glucose levels were significantly higher in yeast-supplemented animals. Serum triglycerides, high density lipoproteins, and low density lipoproteins concentrations were lower, with cholesterol being significantly lower in the treated group. Milk production and milk fat percentage were higher, whereas milk protein percentage and somatic cell count were decreased in yeast-supplemented cows throughout the study. These results suggest that supplementation of S. cerevisiae to dairy cows rations during transition and early lactation period improve their health and milk production parameters., Podaci koji bi se odnosili na uticaj suplementacije probiotika na više parametara istovremeno na istim grlima nedostaju u literaturi. Stoga je cilj ovog eksperimenta bio da se ispita uticaj Saccharomices cerevisiae u obroku/hrani na parametre rumena, krvi i mleka zajedno u visoko-proizvodnim mlečnim govedima tokom tranzicije i početkom laktacije. Šesnaest klinički zdravih holštajn krava je podeljeno u 2 grupe: kontrolna grupa od 6 krava i grupa od 10 krava hranjenih probiotikom u obroku. Buražna tečnost i uzorci krvi su sakupljeni 21 dan pre očekivanog teljenja, kao i 7, 15, 30, 45 i 60 dana tokom laktacije (days in milk - DIM). Prinos mleka za svaku životinju zabeležen je svake 2 nedelje. Pojedinačni uzorci mleka su prikupljeni 15, 30, 45 i 60 DIM. pH buraga i buražni amonijačni azot su bili značajno niži, dok su ukupne isparljive masne kiseline bile značajno veće kod životinja hranjenih kvascem u poređenju sa kontrolama kroz celu studiju. Serumske koncentracije ukupnih proteina i globulina bile su više, dok su koncentracije albumina bile niže u grupi sa kvascem. Serumski nivoi glukoze bili su značajno viši kod životinja sa dodatkom kvasca. Trigliceridi u serumu, koncentracije lipoproteina visoke i niske gustine bile su niže, sa holesterolom koji je bio znatno niži u tretiranoj grupi. Proizvodnja mleka i procent mlečne masti bili su viši, dok je sadržaj proteina mleka i somatskih ćelija bio niži u krava hranjenih sa dodatkom kvasca kroz celu studiju. Ovi rezultati ukazuju na to da dodatak S. cerevisiae u obrocima muznih krava tokom tranzicije i rane laktacije poboljšava njihovo zdravlje i parametre proizvodnje mleka.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Effect of Saccharomyces cerevisiae supplementation on health and performance of dairy cows during transition and early lactation period, Uticaj dodatka Saccharomyces cerevisiae u ishrani na zdravlje i proizvodnju krava tokom perioda tranzicije i početka laktacije",
volume = "31",
number = "3",
pages = "349-364",
doi = "10.2298/BAH1503349B"
}

Bakr, H.A., Hassan, M.S., Giadinis, N., Panousis, N., Ostojić-Andrić, D., Abd, E. M.M.,& Bojkovski, J.. (2015). Effect of Saccharomyces cerevisiae supplementation on health and performance of dairy cows during transition and early lactation period. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 31(3), 349-364.
https://doi.org/10.2298/BAH1503349B

Bakr H, Hassan M, Giadinis N, Panousis N, Ostojić-Andrić D, Abd EM, Bojkovski J. Effect of Saccharomyces cerevisiae supplementation on health and performance of dairy cows during transition and early lactation period. in Biotechnology in Animal Husbandry. 2015;31(3):349-364.
doi:10.2298/BAH1503349B .

Bakr, H.A., Hassan, M.S., Giadinis, Nektarios, Panousis, N., Ostojić-Andrić, Dušica, Abd, El-Tawab M.M., Bojkovski, Jovan, "Effect of Saccharomyces cerevisiae supplementation on health and performance of dairy cows during transition and early lactation period" in Biotechnology in Animal Husbandry, 31, no. 3 (2015):349-364,
https://doi.org/10.2298/BAH1503349B . .

TY  - CONF
AU  - Karasek, S.
AU  - Dokmanović, Marija
AU  - Maksimović, Nevena
AU  - Ostojić Andrić, Dušica
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3985
AB  - Laboratory animals are essential in research, disease diagnostics and
pharmaceutical industry in the production of vaccines and serums, as well as
in potency and safety testing of pharmaceutical products. Like domestic - farm
animals and pets, laboratory animals originate from ancestors that lived in the
wild. For breeding in artifi cial conditions of laboratory and use in experiments
for scientifi c and other purposes rodents are most acceptable. As a rule, these are
animals that are characterized by small size and high fertility, so it is economically
to breed and use.
The most frequent and most important species of laboratory animals is the
mouse. Good knowledge of physiological, medical, genetical, nutritional,
behavioural and other characteristics and needs of laboratory mice is essential
for proper growth and use. Questions and principles of ethics and ensuring the
welfare of laboratory mice in breeding and utilization are important both for
scientifi c and professional as well as for the general public. Principles “3Rs”
obligate scientists and professionals to use their knowledge in the development
of techniques and technologies in breeding and utilization of laboratory mice
in order to be more effective and more humane. In Serbia it is needed to make
technological and ethical improvements in breeding and utilization of laboratory
animals and thus mice, in scientifi c and other purposes, in accordance with
international conventions and scientifi c and professional achievements in the
fi eld of laboratory animal science.
PB  - Belgrade : Faculty of Agriculture
C3  - The First International Symposium on Animal Science (ISAS), Belgrade, November 8 - 10th, 2012
T1  - Breeding and welfare of laboratory mice
SP  - 445
EP  - 451
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3985
ER  - 

@conference{
author = "Karasek, S. and Dokmanović, Marija and Maksimović, Nevena and Ostojić Andrić, Dušica",
year = "2012",
abstract = "Laboratory animals are essential in research, disease diagnostics and
pharmaceutical industry in the production of vaccines and serums, as well as
in potency and safety testing of pharmaceutical products. Like domestic - farm
animals and pets, laboratory animals originate from ancestors that lived in the
wild. For breeding in artifi cial conditions of laboratory and use in experiments
for scientifi c and other purposes rodents are most acceptable. As a rule, these are
animals that are characterized by small size and high fertility, so it is economically
to breed and use.
The most frequent and most important species of laboratory animals is the
mouse. Good knowledge of physiological, medical, genetical, nutritional,
behavioural and other characteristics and needs of laboratory mice is essential
for proper growth and use. Questions and principles of ethics and ensuring the
welfare of laboratory mice in breeding and utilization are important both for
scientifi c and professional as well as for the general public. Principles “3Rs”
obligate scientists and professionals to use their knowledge in the development
of techniques and technologies in breeding and utilization of laboratory mice
in order to be more effective and more humane. In Serbia it is needed to make
technological and ethical improvements in breeding and utilization of laboratory
animals and thus mice, in scientifi c and other purposes, in accordance with
international conventions and scientifi c and professional achievements in the
fi eld of laboratory animal science.",
publisher = "Belgrade : Faculty of Agriculture",
journal = "The First International Symposium on Animal Science (ISAS), Belgrade, November 8 - 10th, 2012",
title = "Breeding and welfare of laboratory mice",
pages = "445-451",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3985"
}

Karasek, S., Dokmanović, M., Maksimović, N.,& Ostojić Andrić, D.. (2012). Breeding and welfare of laboratory mice. in The First International Symposium on Animal Science (ISAS), Belgrade, November 8 - 10th, 2012
Belgrade : Faculty of Agriculture., 445-451.
https://hdl.handle.net/21.15107/rcub_veterinar_3985

Karasek S, Dokmanović M, Maksimović N, Ostojić Andrić D. Breeding and welfare of laboratory mice. in The First International Symposium on Animal Science (ISAS), Belgrade, November 8 - 10th, 2012. 2012;:445-451.
https://hdl.handle.net/21.15107/rcub_veterinar_3985 .

Karasek, S., Dokmanović, Marija, Maksimović, Nevena, Ostojić Andrić, Dušica, "Breeding and welfare of laboratory mice" in The First International Symposium on Animal Science (ISAS), Belgrade, November 8 - 10th, 2012 (2012):445-451,
https://hdl.handle.net/21.15107/rcub_veterinar_3985 .