TY  - JOUR
AU  - Kirovski, Danijela
AU  - Dodovski, Petar
AU  - Savić, Đorđe
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Mirilović, Milorad
AU  - Sladojević, Željko
AU  - Đorđević, Ana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1366
AB  - The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.
AB  - Cilj ovog rada bio je da se ispita da li je tireoidna osovina novorođene teladi promenjena nakon prenatalne aplikacije propiltiouracila (PTU). U ogled je uključeno 20 krava holštajn rase u kasnom graviditetu. Prva grupa (n=10) je tretirana PTU (4 mg/kg telesne mase, dnevna doza) počevši od 20 dana pre očekivanog teljenja do dana teljenja. Druga grupa (n=10) nije tretirana. Uzorci placente krava uzimani su u cilju određivanja ekspresije iRNK dejodinaza tipa I (D1), tipa II (D2) i tipa III (D3). Nakon teljenja, telad su odvojena od majki i uključena u ispitivanja. Uzorci krvi su uzimani dnevno od svakog teleta počevši od dana rođenja do 7. dana života. U krvi su merene koncentracije T3, T4 i TSH. PCR analiza tkiva placente je pokazala zastupljenost sva tri tipa dejodinaza kod netretiranih krava, i značajno povećanje nivoa iRNK sva tri tipa dejodinaza nakon tretmana PTU. Telad koja su poticala od majki tretiranih PTU imala su značajno niže koncentracije T3 i T4 i značajno više koncentracije TSH u odnosu na telad poreklom od netretiranih majki, tokom prva dva dana života. U periodu od 4. do 6. dana života, utvrđen je suprotan efekat, što znači da su telad prenatalno izložena delovanju PTU imala značajno više koncentracije T3 i T4 i značajno niže koncentracije TSH u odnosu na netretiranu telad. Naša studija, po prvi put, daje informacije vezane za ekspresiju dejodinaza u bovinoj placenti i potvrđuje da tretman gravidnih majki sa PTU izaziva depresiju funkcije tireoidne osovine kod novorođenčadi tokom prva dva dana neonatalnog života.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves
T1  - Ekspresija dejodinaza u placenti krava tretiranih sa propiltiouracilom (PTU) i aktivnost tireoidne osovine njihove teladi
VL  - 66
IS  - 1
SP  - 61
EP  - 75
DO  - 10.1515/acve-2016-0005
ER  - 

@article{
author = "Kirovski, Danijela and Dodovski, Petar and Savić, Đorđe and Vujanac, Ivan and Prodanović, Radiša and Mirilović, Milorad and Sladojević, Željko and Đorđević, Ana",
year = "2016",
abstract = "The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life., Cilj ovog rada bio je da se ispita da li je tireoidna osovina novorođene teladi promenjena nakon prenatalne aplikacije propiltiouracila (PTU). U ogled je uključeno 20 krava holštajn rase u kasnom graviditetu. Prva grupa (n=10) je tretirana PTU (4 mg/kg telesne mase, dnevna doza) počevši od 20 dana pre očekivanog teljenja do dana teljenja. Druga grupa (n=10) nije tretirana. Uzorci placente krava uzimani su u cilju određivanja ekspresije iRNK dejodinaza tipa I (D1), tipa II (D2) i tipa III (D3). Nakon teljenja, telad su odvojena od majki i uključena u ispitivanja. Uzorci krvi su uzimani dnevno od svakog teleta počevši od dana rođenja do 7. dana života. U krvi su merene koncentracije T3, T4 i TSH. PCR analiza tkiva placente je pokazala zastupljenost sva tri tipa dejodinaza kod netretiranih krava, i značajno povećanje nivoa iRNK sva tri tipa dejodinaza nakon tretmana PTU. Telad koja su poticala od majki tretiranih PTU imala su značajno niže koncentracije T3 i T4 i značajno više koncentracije TSH u odnosu na telad poreklom od netretiranih majki, tokom prva dva dana života. U periodu od 4. do 6. dana života, utvrđen je suprotan efekat, što znači da su telad prenatalno izložena delovanju PTU imala značajno više koncentracije T3 i T4 i značajno niže koncentracije TSH u odnosu na netretiranu telad. Naša studija, po prvi put, daje informacije vezane za ekspresiju dejodinaza u bovinoj placenti i potvrđuje da tretman gravidnih majki sa PTU izaziva depresiju funkcije tireoidne osovine kod novorođenčadi tokom prva dva dana neonatalnog života.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves, Ekspresija dejodinaza u placenti krava tretiranih sa propiltiouracilom (PTU) i aktivnost tireoidne osovine njihove teladi",
volume = "66",
number = "1",
pages = "61-75",
doi = "10.1515/acve-2016-0005"
}

Kirovski, D., Dodovski, P., Savić, Đ., Vujanac, I., Prodanović, R., Mirilović, M., Sladojević, Ž.,& Đorđević, A.. (2016). Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 61-75.
https://doi.org/10.1515/acve-2016-0005

Kirovski D, Dodovski P, Savić Đ, Vujanac I, Prodanović R, Mirilović M, Sladojević Ž, Đorđević A. Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves. in Acta Veterinaria-Beograd. 2016;66(1):61-75.
doi:10.1515/acve-2016-0005 .

Kirovski, Danijela, Dodovski, Petar, Savić, Đorđe, Vujanac, Ivan, Prodanović, Radiša, Mirilović, Milorad, Sladojević, Željko, Đorđević, Ana, "Placental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calves" in Acta Veterinaria-Beograd, 66, no. 1 (2016):61-75,
https://doi.org/10.1515/acve-2016-0005 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Pantelić, Marija
AU  - Romić, Snjezana
AU  - Stanimirović, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1396
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
SP  - 16
EP  - 19
DO  - 10.1016/j.rvsc.2016.04.007
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana and Pantelić, Marija and Romić, Snjezana and Stanimirović, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
pages = "16-19",
doi = "10.1016/j.rvsc.2016.04.007"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A., Pantelić, M., Romić, S., Stanimirović, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana, Pantelić, Marija, Romić, Snjezana, Stanimirović, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .