TY  - JOUR
AU  - Bošnjak-Neumuller, Jasna
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Kolarević, Stoimir
AU  - Mitić-Ćulafić, Dragana
AU  - Dajic-Stevanović, Zora
AU  - Vukovic-Gacić, Branka
AU  - Knežević-Vukčević, Jelena
AU  - Stanimirović, Zoran
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1548
AB  - There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro
VL  - 49
IS  - 2
SP  - 387
EP  - 397
DO  - 10.2298/GENSR1702387B
ER  - 

@article{
author = "Bošnjak-Neumuller, Jasna and Đelić, Ninoslav and Radaković, Milena and Kolarević, Stoimir and Mitić-Ćulafić, Dragana and Dajic-Stevanović, Zora and Vukovic-Gacić, Branka and Knežević-Vukčević, Jelena and Stanimirović, Zoran",
year = "2017",
abstract = "There is increasing evidence that substances which are normally present in human or animal bodies may, under the certain circumstances, exhibit deleterious effects on genetic material, therefore acting as endogenous mutagenic agents. Since hormones represent one of the best studied endogenous mutagens, some research focused on the possible role of thyroid hormone in mutagenesis and carcinogenesis. Indeed, thyroid hormones accelerate aerobic metabolism and production of reactive oxygen species (ROS) and, therefore, may exhibit mutagenic effects in various test systems on mammalian cells. However, possible mutagenic effects on prokaryotic DNA has not been investigated so far. Hence, the aim of this research was to compare the sensitivity of TA 100 Salmonella typhimurium with and without metabolic activation with S9 fraction, and human lymphocytes to possible genotoxic effects of triiodothyronine (T-3). Therefore, we used the reverse mutation assay on S. typhimurium (Ames test) and in vitro Comet assay in isolated peripheral blood human lymphocytes. In both tests-systems a broad spectrum of T-3 concentrations was applied. The obtained results showed absence of genotoxic effects of T-3 in bacterial reverse mutation assay and very profound genotoxic effects in human lymphocytes at concentrations higher than 15 mu M. We only observed cytotoxic effects in bacterial system at very high T-3 concentrations (300 and 500 mu M). In conclusion, T-3 was unable to increase the level of reverse mutations in Ames test both with and without S9 mix. Therefore, it seems that ROS production in mitochondria may be the primary cause of DNA damage caused by T-3 in mammalian cells.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro",
volume = "49",
number = "2",
pages = "387-397",
doi = "10.2298/GENSR1702387B"
}

Bošnjak-Neumuller, J., Đelić, N., Radaković, M., Kolarević, S., Mitić-Ćulafić, D., Dajic-Stevanović, Z., Vukovic-Gacić, B., Knežević-Vukčević, J.,& Stanimirović, Z.. (2017). Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 49(2), 387-397.
https://doi.org/10.2298/GENSR1702387B

Bošnjak-Neumuller J, Đelić N, Radaković M, Kolarević S, Mitić-Ćulafić D, Dajic-Stevanović Z, Vukovic-Gacić B, Knežević-Vukčević J, Stanimirović Z. Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro. in Genetika. 2017;49(2):387-397.
doi:10.2298/GENSR1702387B .

Bošnjak-Neumuller, Jasna, Đelić, Ninoslav, Radaković, Milena, Kolarević, Stoimir, Mitić-Ćulafić, Dragana, Dajic-Stevanović, Zora, Vukovic-Gacić, Branka, Knežević-Vukčević, Jelena, Stanimirović, Zoran, "Genotoxicity of triiodothyronine: Effects on Salmonella typhimurium TA100 and human lymphocytes in vitro" in Genetika, 49, no. 2 (2017):387-397,
https://doi.org/10.2298/GENSR1702387B . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Stevanović, Jevrosima
AU  - Đelić, Ninoslav
AU  - Lakić, Nada
AU  - Knežević-Vukčević, Jelena
AU  - Vukovic-Gacić, Branka
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1037
AB  - Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.
PB  - Indian Acad Sciences, Bangalore
T2  - Journal of Biosciences
T1  - Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay
VL  - 38
IS  - 1
SP  - 53
EP  - 62
DO  - 10.1007/s12038-012-9287-2
ER  - 

@article{
author = "Radaković, Milena and Stevanović, Jevrosima and Đelić, Ninoslav and Lakić, Nada and Knežević-Vukčević, Jelena and Vukovic-Gacić, Branka and Stanimirović, Zoran",
year = "2013",
abstract = "Amitraz is formamidine pesticide widely used as insecticide and acaricide. In veterinary medicine, amitraz has important uses against ticks, mites and lice on animals. Also, amitraz is used in apiculture to control Varroa destructor. It this study, the alkaline Comet assay was used to evaluate DNA damaging effects of amitraz in human lymphocytes. Isolated human lymphocytes were incubated with varying concentrations of amitraz (0.035, 0.35, 3.5, 35 and 350 mu g/mL). The Comet assay demonstrated that all concentrations of amitraz caused statistically significant increase in the level of DNA damage, thus indicating that amitraz possesses genotoxic potential. The concentration of amitraz that produced the highest DNA damage (3.5 mu g/mL) was chosen for further analysis with the antioxidant catalase. The obtained results showed that co-treatment with antioxidant catalase (100 IU/mL or 500 IU/mL) significantly reduced the level of DNA damage, indicating the possible involvement of reactive oxygen species in DNA damaging effects of amitraz. Flow cytometric analysis revealed increase of the apoptotic index following treatment with amitraz. However, co-treatment with catalase reduced the apoptotic index, while treatment with catalase alone reduced the percentage of apoptotoc cells even in comparison with the negative control. Therefore, catalase had protective effects against ROS-mediated DNA damage and apoptosis.",
publisher = "Indian Acad Sciences, Bangalore",
journal = "Journal of Biosciences",
title = "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay",
volume = "38",
number = "1",
pages = "53-62",
doi = "10.1007/s12038-012-9287-2"
}

Radaković, M., Stevanović, J., Đelić, N., Lakić, N., Knežević-Vukčević, J., Vukovic-Gacić, B.,& Stanimirović, Z.. (2013). Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences
Indian Acad Sciences, Bangalore., 38(1), 53-62.
https://doi.org/10.1007/s12038-012-9287-2

Radaković M, Stevanović J, Đelić N, Lakić N, Knežević-Vukčević J, Vukovic-Gacić B, Stanimirović Z. Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay. in Journal of Biosciences. 2013;38(1):53-62.
doi:10.1007/s12038-012-9287-2 .

Radaković, Milena, Stevanović, Jevrosima, Đelić, Ninoslav, Lakić, Nada, Knežević-Vukčević, Jelena, Vukovic-Gacić, Branka, Stanimirović, Zoran, "Evaluation of the DNA damaging effects of amitraz on human lymphocytes in the Comet assay" in Journal of Biosciences, 38, no. 1 (2013):53-62,
https://doi.org/10.1007/s12038-012-9287-2 . .