TY  - JOUR
AU  - van Hage, M.
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/986
PB  - Wiley, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Diversity of allergens contained in dog saliva REPLY
VL  - 68
IS  - 11
SP  - 1485
EP  - 1486
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_986
ER  - 

@article{
author = "van Hage, M. and Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H.",
year = "2013",
publisher = "Wiley, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Diversity of allergens contained in dog saliva REPLY",
volume = "68",
number = "11",
pages = "1485-1486",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_986"
}

van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T.,& Gronlund, H.. (2013). Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley, Hoboken., 68(11), 1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986

van Hage M, Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H. Diversity of allergens contained in dog saliva REPLY. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(11):1485-1486.
https://hdl.handle.net/21.15107/rcub_veterinar_986 .

van Hage, M., Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., "Diversity of allergens contained in dog saliva REPLY" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 11 (2013):1485-1486,
https://hdl.handle.net/21.15107/rcub_veterinar_986 .

TY  - JOUR
AU  - Polović, N.
AU  - Waden, K.
AU  - Binnmyr, J.
AU  - Hamsten, C.
AU  - Gronneberg, R.
AU  - Palmberg, C.
AU  - Milčić-Matić, Natalija
AU  - Bergman, T.
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1026
AB  - Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
PB  - Wiley-Blackwell, Hoboken
T2  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - an important source of dog allergens
VL  - 68
IS  - 5
SP  - 585
EP  - 592
DO  - 10.1111/all.12130
ER  - 

@article{
author = "Polović, N. and Waden, K. and Binnmyr, J. and Hamsten, C. and Gronneberg, R. and Palmberg, C. and Milčić-Matić, Natalija and Bergman, T. and Gronlund, H. and van Hage, M.",
year = "2013",
abstract = "Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n=13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog danderpositive sera (n=44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - an important source of dog allergens",
volume = "68",
number = "5",
pages = "585-592",
doi = "10.1111/all.12130"
}

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, N., Bergman, T., Gronlund, H.,& van Hage, M.. (2013). Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 68(5), 585-592.
https://doi.org/10.1111/all.12130

Polović N, Waden K, Binnmyr J, Hamsten C, Gronneberg R, Palmberg C, Milčić-Matić N, Bergman T, Gronlund H, van Hage M. Dog saliva - an important source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2013;68(5):585-592.
doi:10.1111/all.12130 .

Polović, N., Waden, K., Binnmyr, J., Hamsten, C., Gronneberg, R., Palmberg, C., Milčić-Matić, Natalija, Bergman, T., Gronlund, H., van Hage, M., "Dog saliva - an important source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 68, no. 5 (2013):585-592,
https://doi.org/10.1111/all.12130 . .

TY  - CONF
AU  - Polović, N.
AU  - Bergman, T.
AU  - Milčić-Matić, Natalija
AU  - Gronlund, H.
AU  - van Hage, M.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/737
AB  - Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.
PB  - Wiley-Blackwell, Hoboken
C3  - Allergy: European Journal of Allergy and Clinical Immunology
T1  - Dog saliva - a source of dog allergens
VL  - 65
IS  - 92
SP  - 105
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_cherry_1464
ER  - 

@conference{
author = "Polović, N. and Bergman, T. and Milčić-Matić, Natalija and Gronlund, H. and van Hage, M.",
year = "2010",
abstract = "Background:Allergy to dog (Canis famili-aris) is recognized worldwide, and it maygive rise to childhood asthma. Can f 1 andCan f 2 together with dog albumin (Can f3) are known allergens in canine. Dog dan-der extract is a poor diagnostic tool ofIgE-mediated allergy to dog. Despite beinga major allergen, Can f 1 is not a verydominant allergen and is therefore insuffi-cient for diagnosis of dog allergy. Theoverall aim of the project is to obtainimproved tools for diagnosis of dog allergyby characterizing and evaluating dog salivaas a new allergen source.Methods:Dog saliva was collected from 14different dog breeds. IgE binding to a dogsaliva pool and to dog dander proteins wascompared in immunoblot using sera from13 dog sensitized patients (SPT and Immu-noCAP positive to dog dander extract)either individually or as a pool. IgE bind-ing proteins present in the dog saliva poolwere identified by MALDI-TOF analysisof  trypsin  in-gel  digested  protein  spotsafter 2D PAGE). The allergenic potentialof different dog breeds was analyzed inimmunoblot using the pool of dog positivesera.Results:In dog dander extract most of thepatients recognized protein bands that cor-responded to already described dog aller-gens (Can f 1, Can f 2 and albumin),whereas in the dog saliva pool at least 12IgE binding proteins  could  be detectedwith molecular weights ranging from 14 to67 kDa.  MALDI-TOF  analysis  of  IgEbinding dander proteins showed the pres-ence of Can f 1 and Can f 2. One addi-tional  IgE  binding  protein  could  bedetected in dog saliva (Can f 1, Can f 2and parotid secretory protein). Differentprotein/allergen profiles could be noted insaliva taken from different dog breeds. E.g. in saliva from Golden Retriever, fewer IgEbinding bands were detected than in salivasamples taken from other dog breeds.Conclusion:It seems to be a greater abun-dance and diversity of IgE binding proteinsin dog saliva compared to dog danderextract. Thus, usage of dog saliva could bean important improvement in the diagnosisof dog allergy.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
title = "Dog saliva - a source of dog allergens",
volume = "65",
number = "92",
pages = "105-105",
url = "https://hdl.handle.net/21.15107/rcub_cherry_1464"
}

Polović, N., Bergman, T., Milčić-Matić, N., Gronlund, H.,& van Hage, M.. (2010). Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology
Wiley-Blackwell, Hoboken., 65(92), 105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464

Polović N, Bergman T, Milčić-Matić N, Gronlund H, van Hage M. Dog saliva - a source of dog allergens. in Allergy: European Journal of Allergy and Clinical Immunology. 2010;65(92):105-105.
https://hdl.handle.net/21.15107/rcub_cherry_1464 .

Polović, N., Bergman, T., Milčić-Matić, Natalija, Gronlund, H., van Hage, M., "Dog saliva - a source of dog allergens" in Allergy: European Journal of Allergy and Clinical Immunology, 65, no. 92 (2010):105-105,
https://hdl.handle.net/21.15107/rcub_cherry_1464 .