TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka Aleksić, Mirjana
AU  - Jovanović Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3864
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka Aleksić, Mirjana and Jovanović Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka Aleksić, M., Jovanović Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka Aleksić M, Jovanović Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka Aleksić, Mirjana, Jovanović Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Vilotić, Aleksandra
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Bojić-Trbojević, Žanka
AU  - Jovanović-Krivokuća, Milica
AU  - Battino, Maurizio
AU  - Giampieri, Francesca
AU  - Dekanski, Dragana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2539
AB  - Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.
PB  - MDPI
T2  - Antioxidants
T1  - Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells
VL  - 12
VL  - 1
SP  - 197
DO  - 10.3390/antiox12010197
ER  - 

@article{
author = "Pirković, Andrea and Vilotić, Aleksandra and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Bojić-Trbojević, Žanka and Jovanović-Krivokuća, Milica and Battino, Maurizio and Giampieri, Francesca and Dekanski, Dragana",
year = "2023",
abstract = "Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast. © 2023 by the authors.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells",
volume = "12, 1",
pages = "197",
doi = "10.3390/antiox12010197"
}

Pirković, A., Vilotić, A., Borozan, S., Nacka-Aleksić, M., Bojić-Trbojević, Ž., Jovanović-Krivokuća, M., Battino, M., Giampieri, F.,& Dekanski, D.. (2023). Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants
MDPI., 12, 197.
https://doi.org/10.3390/antiox12010197

Pirković A, Vilotić A, Borozan S, Nacka-Aleksić M, Bojić-Trbojević Ž, Jovanović-Krivokuća M, Battino M, Giampieri F, Dekanski D. Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. in Antioxidants. 2023;12:197.
doi:10.3390/antiox12010197 .

Pirković, Andrea, Vilotić, Aleksandra, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Bojić-Trbojević, Žanka, Jovanović-Krivokuća, Milica, Battino, Maurizio, Giampieri, Francesca, Dekanski, Dragana, "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells" in Antioxidants, 12 (2023):197,
https://doi.org/10.3390/antiox12010197 . .

TY  - JOUR
AU  - Kostić, Sanja
AU  - Vilotić, Aleksandra
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Vrzić-Petronijević, Svetlana
AU  - Jovanović Krivokuća, Milica
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2842
AB  - Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity
VL  - 163
SP  - 112993
DO  - 10.1016/j.fct.2022.112993
ER  - 

@article{
author = "Kostić, Sanja and Vilotić, Aleksandra and Pirković, Andrea and Dekanski, Dragana and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Vrzić-Petronijević, Svetlana and Jovanović Krivokuća, Milica",
year = "2022",
abstract = "Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity",
volume = "163",
pages = "112993",
doi = "10.1016/j.fct.2022.112993"
}

Kostić, S., Vilotić, A., Pirković, A., Dekanski, D., Borozan, S., Nacka-Aleksić, M., Vrzić-Petronijević, S.,& Jovanović Krivokuća, M.. (2022). Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology
Elsevier., 163, 112993.
https://doi.org/10.1016/j.fct.2022.112993

Kostić S, Vilotić A, Pirković A, Dekanski D, Borozan S, Nacka-Aleksić M, Vrzić-Petronijević S, Jovanović Krivokuća M. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology. 2022;163:112993.
doi:10.1016/j.fct.2022.112993 .

Kostić, Sanja, Vilotić, Aleksandra, Pirković, Andrea, Dekanski, Dragana, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Vrzić-Petronijević, Svetlana, Jovanović Krivokuća, Milica, "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity" in Food and Chemical Toxicology, 163 (2022):112993,
https://doi.org/10.1016/j.fct.2022.112993 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Arsenovic-Ranin, Nevena
AU  - Bufan, Biljana
AU  - Nacka-Aleksić, Mirjana
AU  - Lazarević Macanović, Mirjana
AU  - Milovanović, Petar
AU  - Durić, Marija
AU  - Sopta, Jelena
AU  - Leposavić, Gordana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1665
AB  - Collagen-induced arthritis (CIA) is a frequently used animal model of rheumatoid arthritis, human autoimmune disease that exhibits clear sex bias in incidence and clinical course. Female Dark Agouti rats immunized for CIA showed also greater incidence and higher arthritic score than their male counterparts. The study investigated sex differences in mechanisms controlling the primary immune responses in draining lymph nodes (dLNs), as a factor contributing to this dimorphism. The higher frequencies of CD4 + CD25 + Foxp3- cells, presumably activated effector T (Teff) cells, and IL-17+, IFN-gamma + and IL-17 + IFN-gamma + T cells were found in female compared with male rat dLNs. However, the frequency of CD4 + CD25 + Foxp3 + T regulatory cells (Treg) did not differ between sexes. Thus, CD4 + Teff cells/Treg ratio, and IL-17 + T cells/Treg and IFN-gamma + T cells/Treg ratios were higher in female than in male rats, and among them was found lower frequency of PD-1+ cells. This suggested less efficient control of (auto)immune Th1/Th17 cell responses in female rat dLNs. On the contrary, the frequency of IL-4 + T cells was lower in female than in male rat dLNs. Consistently, the ratio of serum levels of collagen-specific IgG2a (IFN-gamma-dependent, with an important pathogenic role in CIA) and IgG1 (IL-4-dependent) was shifted towards IgG2a in female compared with male rats. As a whole, the study suggests that sexual dimorphism in the control of T cell activation/polarization could contribute to sex bias in the susceptibility to CIA. Moreover, the study advises the use of animals of both sexes in the preclinical testing of new drugs for rheumatoid arthritis.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental and Molecular Pathology
T1  - Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease
VL  - 105
IS  - 1
SP  - 10
EP  - 22
DO  - 10.1016/j.yexmp.2018.05.007
ER  - 

@article{
author = "Dimitrijević, Mirjana and Arsenovic-Ranin, Nevena and Bufan, Biljana and Nacka-Aleksić, Mirjana and Lazarević Macanović, Mirjana and Milovanović, Petar and Durić, Marija and Sopta, Jelena and Leposavić, Gordana",
year = "2018",
abstract = "Collagen-induced arthritis (CIA) is a frequently used animal model of rheumatoid arthritis, human autoimmune disease that exhibits clear sex bias in incidence and clinical course. Female Dark Agouti rats immunized for CIA showed also greater incidence and higher arthritic score than their male counterparts. The study investigated sex differences in mechanisms controlling the primary immune responses in draining lymph nodes (dLNs), as a factor contributing to this dimorphism. The higher frequencies of CD4 + CD25 + Foxp3- cells, presumably activated effector T (Teff) cells, and IL-17+, IFN-gamma + and IL-17 + IFN-gamma + T cells were found in female compared with male rat dLNs. However, the frequency of CD4 + CD25 + Foxp3 + T regulatory cells (Treg) did not differ between sexes. Thus, CD4 + Teff cells/Treg ratio, and IL-17 + T cells/Treg and IFN-gamma + T cells/Treg ratios were higher in female than in male rats, and among them was found lower frequency of PD-1+ cells. This suggested less efficient control of (auto)immune Th1/Th17 cell responses in female rat dLNs. On the contrary, the frequency of IL-4 + T cells was lower in female than in male rat dLNs. Consistently, the ratio of serum levels of collagen-specific IgG2a (IFN-gamma-dependent, with an important pathogenic role in CIA) and IgG1 (IL-4-dependent) was shifted towards IgG2a in female compared with male rats. As a whole, the study suggests that sexual dimorphism in the control of T cell activation/polarization could contribute to sex bias in the susceptibility to CIA. Moreover, the study advises the use of animals of both sexes in the preclinical testing of new drugs for rheumatoid arthritis.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental and Molecular Pathology",
title = "Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease",
volume = "105",
number = "1",
pages = "10-22",
doi = "10.1016/j.yexmp.2018.05.007"
}

Dimitrijević, M., Arsenovic-Ranin, N., Bufan, B., Nacka-Aleksić, M., Lazarević Macanović, M., Milovanović, P., Durić, M., Sopta, J.,& Leposavić, G.. (2018). Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease. in Experimental and Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 105(1), 10-22.
https://doi.org/10.1016/j.yexmp.2018.05.007

Dimitrijević M, Arsenovic-Ranin N, Bufan B, Nacka-Aleksić M, Lazarević Macanović M, Milovanović P, Durić M, Sopta J, Leposavić G. Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease. in Experimental and Molecular Pathology. 2018;105(1):10-22.
doi:10.1016/j.yexmp.2018.05.007 .

Dimitrijević, Mirjana, Arsenovic-Ranin, Nevena, Bufan, Biljana, Nacka-Aleksić, Mirjana, Lazarević Macanović, Mirjana, Milovanović, Petar, Durić, Marija, Sopta, Jelena, Leposavić, Gordana, "Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease" in Experimental and Molecular Pathology, 105, no. 1 (2018):10-22,
https://doi.org/10.1016/j.yexmp.2018.05.007 . .