TY  - CONF
AU  - Nenadović, Katarina
AU  - Maletić, Milan
AU  - Pauković, Dragiša
AU  - Đorđević, Milutin
AU  - Janković, Ljiljana
AU  - Fratrić, Natalija
AU  - Aleksić Radojković, Jelena
AU  - Vučinić, Marijana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3215
AB  - Upotreba parametara reprodukcije kao indikatora dobrobiti životinja ima
ograničenja i zato ove parametre treba posmatrati samo kao indirektne indikatore
dobrobiti. Međutim, reprodukcija može imati direktne negativne efekte na dobrobit
životinja kroz distokiju, abortus, metritis i drugo. Na nivou farme, stopa fertiliteta
naglašava samo biološke performanse stada, ali ne uzima u obzir dobrobit pojedinih
životinja. Dobar reproduktivni učinak ne znači automatski zadovoljavajuću dobrobit
životinja, pošto su domestikacija i ciljani programi uzgoja doveli do davanja prioriteta
visokim produktivnim i reproduktivnim performansama kod većine savremenih vrsta
životinja na farmi. Smanjen reproduktivni učinak na farmama goveda može ukazivati
na prisustvo problema koja se odnose i na dobrobit životinja. U mnogim istraživanjima
je utvrđeno da prenaseljenost, loši higijenski uslovi, toplotni stres i nemogućnost
ispoljavanja prirodnih oblika ponašanja kao što je ispaša utiču na lučenje
adrenokortikotropnog hormona (ACTH) i kortizola. Neuravnoteženo lučenje ovih
hormona može izazvati poremećaj ciklusa jajnika i i uticati na imunski odgovor
reproduktivnog trakta. Visok rizik od ovih poremećaja može uzrokovati smanjene
performanse reprodukcije kao što je produžen servis period, smanjena stopa začeća i
povećan interval teljenja. Sa druge strane, veštačko osemenjavanje može pružiti
potencijalne prednosti za dobrobit životinja jer ova praksa smanjuje rizik od
prenošenja bolesti i povreda i omogućava odabir specifičnih performansi koje
potomstvo nasleđuje od bika. Nasuprot tome, sposobnost životinja da ispolji
reproduktivno ponašanje kao što je ekspresija estrusa, aspekt koji može da doprinese dobrobiti životinja, često je ograničen na komercijalnim farmama koje je posebno
evidentno u vezanom sistemu gajenja goveda.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Odnos između dobrobiti životinja i reprodukcije goveda
SP  - 78
EP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3215
ER  - 

@conference{
author = "Nenadović, Katarina and Maletić, Milan and Pauković, Dragiša and Đorđević, Milutin and Janković, Ljiljana and Fratrić, Natalija and Aleksić Radojković, Jelena and Vučinić, Marijana",
year = "2023",
abstract = "Upotreba parametara reprodukcije kao indikatora dobrobiti životinja ima
ograničenja i zato ove parametre treba posmatrati samo kao indirektne indikatore
dobrobiti. Međutim, reprodukcija može imati direktne negativne efekte na dobrobit
životinja kroz distokiju, abortus, metritis i drugo. Na nivou farme, stopa fertiliteta
naglašava samo biološke performanse stada, ali ne uzima u obzir dobrobit pojedinih
životinja. Dobar reproduktivni učinak ne znači automatski zadovoljavajuću dobrobit
životinja, pošto su domestikacija i ciljani programi uzgoja doveli do davanja prioriteta
visokim produktivnim i reproduktivnim performansama kod većine savremenih vrsta
životinja na farmi. Smanjen reproduktivni učinak na farmama goveda može ukazivati
na prisustvo problema koja se odnose i na dobrobit životinja. U mnogim istraživanjima
je utvrđeno da prenaseljenost, loši higijenski uslovi, toplotni stres i nemogućnost
ispoljavanja prirodnih oblika ponašanja kao što je ispaša utiču na lučenje
adrenokortikotropnog hormona (ACTH) i kortizola. Neuravnoteženo lučenje ovih
hormona može izazvati poremećaj ciklusa jajnika i i uticati na imunski odgovor
reproduktivnog trakta. Visok rizik od ovih poremećaja može uzrokovati smanjene
performanse reprodukcije kao što je produžen servis period, smanjena stopa začeća i
povećan interval teljenja. Sa druge strane, veštačko osemenjavanje može pružiti
potencijalne prednosti za dobrobit životinja jer ova praksa smanjuje rizik od
prenošenja bolesti i povreda i omogućava odabir specifičnih performansi koje
potomstvo nasleđuje od bika. Nasuprot tome, sposobnost životinja da ispolji
reproduktivno ponašanje kao što je ekspresija estrusa, aspekt koji može da doprinese dobrobiti životinja, često je ograničen na komercijalnim farmama koje je posebno
evidentno u vezanom sistemu gajenja goveda.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Odnos između dobrobiti životinja i reprodukcije goveda",
pages = "78-89",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3215"
}

Nenadović, K., Maletić, M., Pauković, D., Đorđević, M., Janković, L., Fratrić, N., Aleksić Radojković, J.,& Vučinić, M.. (2023). Odnos između dobrobiti životinja i reprodukcije goveda. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 78-89.
https://hdl.handle.net/21.15107/rcub_veterinar_3215

Nenadović K, Maletić M, Pauković D, Đorđević M, Janković L, Fratrić N, Aleksić Radojković J, Vučinić M. Odnos između dobrobiti životinja i reprodukcije goveda. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:78-89.
https://hdl.handle.net/21.15107/rcub_veterinar_3215 .

Nenadović, Katarina, Maletić, Milan, Pauković, Dragiša, Đorđević, Milutin, Janković, Ljiljana, Fratrić, Natalija, Aleksić Radojković, Jelena, Vučinić, Marijana, "Odnos između dobrobiti životinja i reprodukcije goveda" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):78-89,
https://hdl.handle.net/21.15107/rcub_veterinar_3215 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Nenadović, Katarina
AU  - Maletić, Milan
AU  - Bugarski, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3213
AB  - Razumevanje uslova tokom intrauterinog razvoja koja utiču na zdravlje i rast
teladi može pomoći u osmišljavanju prakse menadžmenta koja pozitivno utiče na
razvoj novorođenčadi i buduće proizvodne rezultate krava. Više faktora majke je
dokazano da utiče na epigenom fetusa, kao što su ishrana, metabolički status, toksini
(pesticidi ili herbicidi), socijalna interakcija i stresori sredine ( na pr., toplotni stres).
Oksidativni stres je takođe važan faktor koji pored promena u ekspresiji gena i
proteina, može doprineti stvaranju neadekvatnog okruženja za razvoj fetusa usled
promena u funkciji metabolizma majke. Period kasne gestacije je period najvećeg
fetalnog rasta i najbrže proliferacije imunskih ćelija fetusa goveda. Stoga suboptimalni
uslovi u predhodnim I ovom periodu gestacije mogu dovesti do promena u strukturi i
funkciji tkiva I organa koje su u vezi sa metabolizmom, rastom I većom osetljivosti na
bolesti teladi a održavaju se I na produktivnost kao odrasle jedinke.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Uticaj stresa tokom kasne gestacije na rast, zdravlje teladi mlečnih krava i proizvodne rezultate kao odrasle jedinke
SP  - 53
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3213
ER  - 

@conference{
author = "Fratrić, Natalija and Gvozdić, Dragan and Nenadović, Katarina and Maletić, Milan and Bugarski, Dejan",
year = "2023",
abstract = "Razumevanje uslova tokom intrauterinog razvoja koja utiču na zdravlje i rast
teladi može pomoći u osmišljavanju prakse menadžmenta koja pozitivno utiče na
razvoj novorođenčadi i buduće proizvodne rezultate krava. Više faktora majke je
dokazano da utiče na epigenom fetusa, kao što su ishrana, metabolički status, toksini
(pesticidi ili herbicidi), socijalna interakcija i stresori sredine ( na pr., toplotni stres).
Oksidativni stres je takođe važan faktor koji pored promena u ekspresiji gena i
proteina, može doprineti stvaranju neadekvatnog okruženja za razvoj fetusa usled
promena u funkciji metabolizma majke. Period kasne gestacije je period najvećeg
fetalnog rasta i najbrže proliferacije imunskih ćelija fetusa goveda. Stoga suboptimalni
uslovi u predhodnim I ovom periodu gestacije mogu dovesti do promena u strukturi i
funkciji tkiva I organa koje su u vezi sa metabolizmom, rastom I većom osetljivosti na
bolesti teladi a održavaju se I na produktivnost kao odrasle jedinke.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Uticaj stresa tokom kasne gestacije na rast, zdravlje teladi mlečnih krava i proizvodne rezultate kao odrasle jedinke",
pages = "53-61",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3213"
}

Fratrić, N., Gvozdić, D., Nenadović, K., Maletić, M.,& Bugarski, D.. (2023). Uticaj stresa tokom kasne gestacije na rast, zdravlje teladi mlečnih krava i proizvodne rezultate kao odrasle jedinke. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 53-61.
https://hdl.handle.net/21.15107/rcub_veterinar_3213

Fratrić N, Gvozdić D, Nenadović K, Maletić M, Bugarski D. Uticaj stresa tokom kasne gestacije na rast, zdravlje teladi mlečnih krava i proizvodne rezultate kao odrasle jedinke. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:53-61.
https://hdl.handle.net/21.15107/rcub_veterinar_3213 .

Fratrić, Natalija, Gvozdić, Dragan, Nenadović, Katarina, Maletić, Milan, Bugarski, Dejan, "Uticaj stresa tokom kasne gestacije na rast, zdravlje teladi mlečnih krava i proizvodne rezultate kao odrasle jedinke" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):53-61,
https://hdl.handle.net/21.15107/rcub_veterinar_3213 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3280
AB  - Mikrobiom goveda predstavlja dinamičan ekosistem koji podleže značajnim
fluktuacijama tokom celog života jedinke. Sastav mikrobioma varira u zavisnosti od faktora domaćina i različitih fizičko-hemijskih osobina sredine koju naseljava. Mikrobiom kod goveda čine veoma veliki broj vrsta mikroorganizama koji žive
i koegzistiraju u/na prirodnom domaćinu, a poremećaji njihovih populacija mogu
imati štetan efekat na zdravlje. Ravnoteža rasta mikrobne populacije može biti poremećena u toku bolesti usled smanjenja istih, ili pojave različitih vrsta mikroba
što dovodi do disbioze. Disbioza može izazvati ili pogoršati više bolesti kod goveda. Mogući izvori mikroflore u reproduktivnom traktu su prenos bakterija iz creva
(feces), iz spoljašnje sredine (prostirka, koža), najčešće vaginalnim putem, kao i
hematogenim putem. Značajne razlike su ustanovljene u mikrobiomu reproduktivnog trakta goveda u zavisnosti od rase, anatomske regije i fiziološkog stanja. Otkriveno je da je mikrobiom reproduktivnog trakta majke povezan sa ishodom graviditeta i kasnijim ranim životom potomstva.
Osim tokom teljenja, patogeni mikroorganizmi mogu imati pristup reproduktivnim organima tokom parenja i veštačkog osemenjavanja. Invazija patogenih
mikroba izaziva inflamatorni odgovor i uništavanje integriteta endometrijuma, što
je nepovoljno za transport spermatozoida i razvoj embriona. Promena komensalnih bakterija unutar mlečne žlezde ima štetan efekat na homeostazu mlečne žlezde. Poznavanje mikrobioma reproduktivnog trakta goveda može imati veliki značaj za uspešnu reprodukciju goveda, ishod terapijskih procedura i zdravlje novorođenih teladi.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
T1  - Mikrobiom kod zdravih goveda i poremećaji (disbioza) kod metritisa, endometritisa i mastitisa
SP  - 37
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3280
ER  - 

@conference{
author = "Fratrić, Natalija and Gvozdić, Dragan",
year = "2023",
abstract = "Mikrobiom goveda predstavlja dinamičan ekosistem koji podleže značajnim
fluktuacijama tokom celog života jedinke. Sastav mikrobioma varira u zavisnosti od faktora domaćina i različitih fizičko-hemijskih osobina sredine koju naseljava. Mikrobiom kod goveda čine veoma veliki broj vrsta mikroorganizama koji žive
i koegzistiraju u/na prirodnom domaćinu, a poremećaji njihovih populacija mogu
imati štetan efekat na zdravlje. Ravnoteža rasta mikrobne populacije može biti poremećena u toku bolesti usled smanjenja istih, ili pojave različitih vrsta mikroba
što dovodi do disbioze. Disbioza može izazvati ili pogoršati više bolesti kod goveda. Mogući izvori mikroflore u reproduktivnom traktu su prenos bakterija iz creva
(feces), iz spoljašnje sredine (prostirka, koža), najčešće vaginalnim putem, kao i
hematogenim putem. Značajne razlike su ustanovljene u mikrobiomu reproduktivnog trakta goveda u zavisnosti od rase, anatomske regije i fiziološkog stanja. Otkriveno je da je mikrobiom reproduktivnog trakta majke povezan sa ishodom graviditeta i kasnijim ranim životom potomstva.
Osim tokom teljenja, patogeni mikroorganizmi mogu imati pristup reproduktivnim organima tokom parenja i veštačkog osemenjavanja. Invazija patogenih
mikroba izaziva inflamatorni odgovor i uništavanje integriteta endometrijuma, što
je nepovoljno za transport spermatozoida i razvoj embriona. Promena komensalnih bakterija unutar mlečne žlezde ima štetan efekat na homeostazu mlečne žlezde. Poznavanje mikrobioma reproduktivnog trakta goveda može imati veliki značaj za uspešnu reprodukciju goveda, ishod terapijskih procedura i zdravlje novorođenih teladi.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023",
title = "Mikrobiom kod zdravih goveda i poremećaji (disbioza) kod metritisa, endometritisa i mastitisa",
pages = "37-52",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3280"
}

Fratrić, N.,& Gvozdić, D.. (2023). Mikrobiom kod zdravih goveda i poremećaji (disbioza) kod metritisa, endometritisa i mastitisa. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 37-52.
https://hdl.handle.net/21.15107/rcub_veterinar_3280

Fratrić N, Gvozdić D. Mikrobiom kod zdravih goveda i poremećaji (disbioza) kod metritisa, endometritisa i mastitisa. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023. 2023;:37-52.
https://hdl.handle.net/21.15107/rcub_veterinar_3280 .

Fratrić, Natalija, Gvozdić, Dragan, "Mikrobiom kod zdravih goveda i poremećaji (disbioza) kod metritisa, endometritisa i mastitisa" in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023 (2023):37-52,
https://hdl.handle.net/21.15107/rcub_veterinar_3280 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Gvozdić, Dragan
AU  - Marković, Dragana
AU  - Drvenica, Ivana
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3331
PB  - German Veterinary Medical Society
C3  - European Buiatrics Congress and ECBHM Jubilee Symposium 2023, Berlin, Germany, August 24 - 26, 2023
T1  - Rivanol soluble blood serum IgG in calf bronchopneumonia
SP  - 251
EP  - 252
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3331
ER  - 

@conference{
author = "Fratrić, Natalija and Kovačić, Marijana and Gvozdić, Dragan and Marković, Dragana and Drvenica, Ivana and Maslovarić, Irina and Ilić, Vesna",
year = "2023",
publisher = "German Veterinary Medical Society",
journal = "European Buiatrics Congress and ECBHM Jubilee Symposium 2023, Berlin, Germany, August 24 - 26, 2023",
title = "Rivanol soluble blood serum IgG in calf bronchopneumonia",
pages = "251-252",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3331"
}

Fratrić, N., Kovačić, M., Gvozdić, D., Marković, D., Drvenica, I., Maslovarić, I.,& Ilić, V.. (2023). Rivanol soluble blood serum IgG in calf bronchopneumonia. in European Buiatrics Congress and ECBHM Jubilee Symposium 2023, Berlin, Germany, August 24 - 26, 2023
German Veterinary Medical Society., 251-252.
https://hdl.handle.net/21.15107/rcub_veterinar_3331

Fratrić N, Kovačić M, Gvozdić D, Marković D, Drvenica I, Maslovarić I, Ilić V. Rivanol soluble blood serum IgG in calf bronchopneumonia. in European Buiatrics Congress and ECBHM Jubilee Symposium 2023, Berlin, Germany, August 24 - 26, 2023. 2023;:251-252.
https://hdl.handle.net/21.15107/rcub_veterinar_3331 .

Fratrić, Natalija, Kovačić, Marijana, Gvozdić, Dragan, Marković, Dragana, Drvenica, Ivana, Maslovarić, Irina, Ilić, Vesna, "Rivanol soluble blood serum IgG in calf bronchopneumonia" in European Buiatrics Congress and ECBHM Jubilee Symposium 2023, Berlin, Germany, August 24 - 26, 2023 (2023):251-252,
https://hdl.handle.net/21.15107/rcub_veterinar_3331 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Andjelić, Biljana
AU  - Djoković, Radojica
AU  - Cincović, Marko
AU  - Čukić, Aleksandar
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3332
AB  - The objective of the present study was to estimate metabolic status in Holstein dairy
cows during different stages of lactation, on the basis changes of blood
concentrations of selected biochemical markers. Blood samples were collected from
100 Holstein dairy cows and blood serum parameters were evaluated. The cows
were allocated to four groups according to the production period, including Group 1 –
cows in early lactation (n=18), Group 2 – cows in full lactation (n=26), Group 3 –
cows in mid lactation (n=25), and Group 4 – cows in late lactation (n=31). Statistically
significant differences (P < 0.01) were found between the experimental groups of
cows in blood NEFA, BHB, TP, TG, TChol, urea, globulin, levels, and enzyme
activities of AST, ALT, ALP, GGT and LDH. The intensity of lipomobilisation (NEFA
or BHB concentrations) correlated positively (P < 0.05) with the markers of cell
damage or liver function impairment (tBIL.,serum AST, ALT, LDH and GGT
activities), but negatively (P < 0.05) with the circulating concentrations of compounds
synthesised in liver (glucose, TG and urea). All these biochemical metabolites may
be used as important biochemical markers in the determination of the metabolic
status in high-yielding dairy cows.
PB  - Stara Zagora, Bulgaria : Faculty of Veterinary Medicine
C3  - XXII Middle European Buiatric Congress, Stara Zagora, Bulgaria, 31st of  May to 3rd of June 2023
T1  - Evaluation of metabolic status in the Holstein dairy cows during different stages of lactation
SP  - 75
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3332
ER  - 

@conference{
author = "Fratrić, Natalija and Andjelić, Biljana and Djoković, Radojica and Cincović, Marko and Čukić, Aleksandar",
year = "2023",
abstract = "The objective of the present study was to estimate metabolic status in Holstein dairy
cows during different stages of lactation, on the basis changes of blood
concentrations of selected biochemical markers. Blood samples were collected from
100 Holstein dairy cows and blood serum parameters were evaluated. The cows
were allocated to four groups according to the production period, including Group 1 –
cows in early lactation (n=18), Group 2 – cows in full lactation (n=26), Group 3 –
cows in mid lactation (n=25), and Group 4 – cows in late lactation (n=31). Statistically
significant differences (P < 0.01) were found between the experimental groups of
cows in blood NEFA, BHB, TP, TG, TChol, urea, globulin, levels, and enzyme
activities of AST, ALT, ALP, GGT and LDH. The intensity of lipomobilisation (NEFA
or BHB concentrations) correlated positively (P < 0.05) with the markers of cell
damage or liver function impairment (tBIL.,serum AST, ALT, LDH and GGT
activities), but negatively (P < 0.05) with the circulating concentrations of compounds
synthesised in liver (glucose, TG and urea). All these biochemical metabolites may
be used as important biochemical markers in the determination of the metabolic
status in high-yielding dairy cows.",
publisher = "Stara Zagora, Bulgaria : Faculty of Veterinary Medicine",
journal = "XXII Middle European Buiatric Congress, Stara Zagora, Bulgaria, 31st of  May to 3rd of June 2023",
title = "Evaluation of metabolic status in the Holstein dairy cows during different stages of lactation",
pages = "75-75",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3332"
}

Fratrić, N., Andjelić, B., Djoković, R., Cincović, M.,& Čukić, A.. (2023). Evaluation of metabolic status in the Holstein dairy cows during different stages of lactation. in XXII Middle European Buiatric Congress, Stara Zagora, Bulgaria, 31st of  May to 3rd of June 2023
Stara Zagora, Bulgaria : Faculty of Veterinary Medicine., 75-75.
https://hdl.handle.net/21.15107/rcub_veterinar_3332

Fratrić N, Andjelić B, Djoković R, Cincović M, Čukić A. Evaluation of metabolic status in the Holstein dairy cows during different stages of lactation. in XXII Middle European Buiatric Congress, Stara Zagora, Bulgaria, 31st of  May to 3rd of June 2023. 2023;:75-75.
https://hdl.handle.net/21.15107/rcub_veterinar_3332 .

Fratrić, Natalija, Andjelić, Biljana, Djoković, Radojica, Cincović, Marko, Čukić, Aleksandar, "Evaluation of metabolic status in the Holstein dairy cows during different stages of lactation" in XXII Middle European Buiatric Congress, Stara Zagora, Bulgaria, 31st of  May to 3rd of June 2023 (2023):75-75,
https://hdl.handle.net/21.15107/rcub_veterinar_3332 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vakanjac, Slobodanka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3182
AB  - Uzgoj junica za remont stada na farmamma mlečnih krava predstavlja značajan
trošak, pri čemu je reproduktivna efikasnost jedan od glavnih faktora koji utiču
na ukupne troškove uzgoja. Smanjen prirast kod teladi, smanjena proizvodnja
mleka kod krava koje su kao telad imale hronične bolesti, širenje zaraznih bolesti
sa teladi na odrasle krave, povećani veterinarski troškovi, ograničena mogućnost
genetske selekcije zbog visokog mortaliteta životinja za obrt stada, negativno utiče
na produktivnost. Najveći morbiditet i mortalitet se javlja kod teladi pre odbijanja.
Mnoge bolesti novorođenih teladi mogu se kontrolisati dobro osmišljenim programima
upravljanja zdravljem, koji definišu brigu i smeštaj majke tokom peripartalnog
perioda, standardne operativne procedure kod teljenja i primenu odgovarajućih
preventivnih mera (uključujući programe pravilne ishrane) za novorođenu
telad. Zdrava telad mogu postići stopu rasta koja im omogućava polnu zrelost u
starosti od 13 do 15 meseci što se pozitivno odražava na produktivnost celokupnog
stada mlečnih krava.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022
T1  - Glavni zdravstveni problemi kod teladi u neonatalnom periodu
SP  - 17
EP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3182
ER  - 

@conference{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vakanjac, Slobodanka",
year = "2022",
abstract = "Uzgoj junica za remont stada na farmamma mlečnih krava predstavlja značajan
trošak, pri čemu je reproduktivna efikasnost jedan od glavnih faktora koji utiču
na ukupne troškove uzgoja. Smanjen prirast kod teladi, smanjena proizvodnja
mleka kod krava koje su kao telad imale hronične bolesti, širenje zaraznih bolesti
sa teladi na odrasle krave, povećani veterinarski troškovi, ograničena mogućnost
genetske selekcije zbog visokog mortaliteta životinja za obrt stada, negativno utiče
na produktivnost. Najveći morbiditet i mortalitet se javlja kod teladi pre odbijanja.
Mnoge bolesti novorođenih teladi mogu se kontrolisati dobro osmišljenim programima
upravljanja zdravljem, koji definišu brigu i smeštaj majke tokom peripartalnog
perioda, standardne operativne procedure kod teljenja i primenu odgovarajućih
preventivnih mera (uključujući programe pravilne ishrane) za novorođenu
telad. Zdrava telad mogu postići stopu rasta koja im omogućava polnu zrelost u
starosti od 13 do 15 meseci što se pozitivno odražava na produktivnost celokupnog
stada mlečnih krava.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022",
title = "Glavni zdravstveni problemi kod teladi u neonatalnom periodu",
pages = "17-29",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3182"
}

Fratrić, N., Gvozdić, D.,& Vakanjac, S.. (2022). Glavni zdravstveni problemi kod teladi u neonatalnom periodu. in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 17-29.
https://hdl.handle.net/21.15107/rcub_veterinar_3182

Fratrić N, Gvozdić D, Vakanjac S. Glavni zdravstveni problemi kod teladi u neonatalnom periodu. in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022. 2022;:17-29.
https://hdl.handle.net/21.15107/rcub_veterinar_3182 .

Fratrić, Natalija, Gvozdić, Dragan, Vakanjac, Slobodanka, "Glavni zdravstveni problemi kod teladi u neonatalnom periodu" in 13. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 6 - 9. oktobar 2022 (2022):17-29,
https://hdl.handle.net/21.15107/rcub_veterinar_3182 .

TY  - JOUR
AU  - Ivana, Drvenica
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Grujić-Milanović, Jelica
AU  - Vejnović, Branislav
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2646
AB  - Redox imbalance in peripartum period influences health of dairy cows and their foetus and,
through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed
by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant
primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching,
with 1 L of water containing either 0 g/L (n ¼ 14; control group) or 150 g/L of clinoptilolite (n ¼ 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to
parturition and on days 1 (þ1 D), 2 (þ2 D), and 7 (þ7 D) pp, and colostrum were collected at 2,
12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein
products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum
whey. The concentration of antioxidants in the peripheral blood of supplemented cows was
increased by 41% and 19% on (þ2 D) and (þ7 D), respectively, while the concentration of lipid
peroxides on (þ7 D) was lowered by 57% compared with the control group. In addition, this
supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides
level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation
with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of
primiparous cows to redox imbalance in the peripartum period.
PB  - Taylor and Francis
T2  - Italian Journal of Animal Science
T1  - Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows
VL  - 21
IS  - 1
SP  - 847
EP  - 858
DO  - 10.1080/1828051X.2022.2070034
ER  - 

@article{
author = "Ivana, Drvenica and Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Grujić-Milanović, Jelica and Vejnović, Branislav and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna",
year = "2022",
abstract = "Redox imbalance in peripartum period influences health of dairy cows and their foetus and,
through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed
by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant
primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching,
with 1 L of water containing either 0 g/L (n ¼ 14; control group) or 150 g/L of clinoptilolite (n ¼ 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to
parturition and on days 1 (þ1 D), 2 (þ2 D), and 7 (þ7 D) pp, and colostrum were collected at 2,
12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein
products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum
whey. The concentration of antioxidants in the peripheral blood of supplemented cows was
increased by 41% and 19% on (þ2 D) and (þ7 D), respectively, while the concentration of lipid
peroxides on (þ7 D) was lowered by 57% compared with the control group. In addition, this
supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides
level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation
with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of
primiparous cows to redox imbalance in the peripartum period.",
publisher = "Taylor and Francis",
journal = "Italian Journal of Animal Science",
title = "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows",
volume = "21",
number = "1",
pages = "847-858",
doi = "10.1080/1828051X.2022.2070034"
}

Ivana, D., Stojić, M., Fratrić, N., Kovačić, M., Grujić-Milanović, J., Vejnović, B., Marković, D., Gvozdić, D.,& Ilić, V.. (2022). Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science
Taylor and Francis., 21(1), 847-858.
https://doi.org/10.1080/1828051X.2022.2070034

Ivana D, Stojić M, Fratrić N, Kovačić M, Grujić-Milanović J, Vejnović B, Marković D, Gvozdić D, Ilić V. Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science. 2022;21(1):847-858.
doi:10.1080/1828051X.2022.2070034 .

Ivana, Drvenica, Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Grujić-Milanović, Jelica, Vejnović, Branislav, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows" in Italian Journal of Animal Science, 21, no. 1 (2022):847-858,
https://doi.org/10.1080/1828051X.2022.2070034 . .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Vakanjac, Slobodanka
AU  - Stojić, Milica
AU  - Gvozdić, Dragan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3333
AB  - Primena „precizne tehnologije” u uzgoju mlečnih krava (engl. precision dairy
farming technologies, PDF) predstavlja model upravljanja farmom uz neprekidnu
podršku automatizovanih sistema za praćenje različitih parametara proizvodnje,
reprodukcije, zdravlja i dobrobiti životinja, kao i mogućih uticaja na životnu sredinu. Ovaj model upravljanja farmom je većinom baziran na upotrebi digitalne tehnologije. To podrazumeva korišćenje adekvatnih mernih instrumenata (senzora,
kamera, mikrofona, skala) i analitičkih uređaja (specifičnih softverskih paketa) koji transformišu dobijene podatke u informaciju koja je korisna za uzgajivača. Na
farmama mlečnih krava, PDF omogućava da se u realnom vremenu rukuje velikim brojem podataka iz različitih sistema, na osnovu kojih se mogu donositi važne odluke o upravljanu farmom. Podaci koji se dobijaju aktivnošću senzora mogu
se koristiti za praćenje proizvodnih rezultata (mlečnost, kvalitet mleka), dobrobiti
i adekvatnog smeštaja (spoljašnja temperatura, vlažnost vazduha), kao fiziološkog stanja krava (detekcija estrusa, vreme teljenja), problema sa zdravljem (hromost, mastitis, metritis) i metaboličkih problema koji su u vezi sa ishranom. Automatizovani sistemi za praćenje motoričke aktivnosti su najčešće korišćeni sistemi
za otkrivanje estrusa kod krava. Praćenjem motoričke aktivnosti putem senzora,
mogu se korektno predvideti ovulacija i pravo vreme za veštačko osemenjavanje. Na ovaj način se može otkriti 70-80 % krava koje su u estrusu, a mogu se
dobiti i informacije o trajanju i intenzitetu estrusa koje su korisne pri odlučivanju o
vremenu osemenjavanja. Protokoli kontrole reprodukcije, zasnovani na otkrivanju
estrusa pomoću praćenja motoričke aktivnosti (AAM) mogu biti veoma uspešni i
uporedivi sa intenzivnim programima „vremenski ograničenog“ veštačkog osemenjavanja (engl. timed AI protocol, TAI), koji uključuje tretmane gonadotropnim releasing-hormonom (GnRH) i prostaglandinima (PGF2α). Takođe je moguće praćenje i nadgledanje teljenja „na daljinu”, bez uznemiravanja životinje i dodatnog
stresa. Time se omogućava brži oporavak i brže uvođenje u reprodukciju, što se
odražava na povećanje proizvodnje mleka, smanjenje troškova osemenjavanja i
veći broj teladi.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 07 - 10. oktobar 2021
T1  - Digitalne tehnologije i reprodukcija krava
SP  - 43
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3333
ER  - 

@conference{
author = "Fratrić, Natalija and Vakanjac, Slobodanka and Stojić, Milica and Gvozdić, Dragan",
year = "2021",
abstract = "Primena „precizne tehnologije” u uzgoju mlečnih krava (engl. precision dairy
farming technologies, PDF) predstavlja model upravljanja farmom uz neprekidnu
podršku automatizovanih sistema za praćenje različitih parametara proizvodnje,
reprodukcije, zdravlja i dobrobiti životinja, kao i mogućih uticaja na životnu sredinu. Ovaj model upravljanja farmom je većinom baziran na upotrebi digitalne tehnologije. To podrazumeva korišćenje adekvatnih mernih instrumenata (senzora,
kamera, mikrofona, skala) i analitičkih uređaja (specifičnih softverskih paketa) koji transformišu dobijene podatke u informaciju koja je korisna za uzgajivača. Na
farmama mlečnih krava, PDF omogućava da se u realnom vremenu rukuje velikim brojem podataka iz različitih sistema, na osnovu kojih se mogu donositi važne odluke o upravljanu farmom. Podaci koji se dobijaju aktivnošću senzora mogu
se koristiti za praćenje proizvodnih rezultata (mlečnost, kvalitet mleka), dobrobiti
i adekvatnog smeštaja (spoljašnja temperatura, vlažnost vazduha), kao fiziološkog stanja krava (detekcija estrusa, vreme teljenja), problema sa zdravljem (hromost, mastitis, metritis) i metaboličkih problema koji su u vezi sa ishranom. Automatizovani sistemi za praćenje motoričke aktivnosti su najčešće korišćeni sistemi
za otkrivanje estrusa kod krava. Praćenjem motoričke aktivnosti putem senzora,
mogu se korektno predvideti ovulacija i pravo vreme za veštačko osemenjavanje. Na ovaj način se može otkriti 70-80 % krava koje su u estrusu, a mogu se
dobiti i informacije o trajanju i intenzitetu estrusa koje su korisne pri odlučivanju o
vremenu osemenjavanja. Protokoli kontrole reprodukcije, zasnovani na otkrivanju
estrusa pomoću praćenja motoričke aktivnosti (AAM) mogu biti veoma uspešni i
uporedivi sa intenzivnim programima „vremenski ograničenog“ veštačkog osemenjavanja (engl. timed AI protocol, TAI), koji uključuje tretmane gonadotropnim releasing-hormonom (GnRH) i prostaglandinima (PGF2α). Takođe je moguće praćenje i nadgledanje teljenja „na daljinu”, bez uznemiravanja životinje i dodatnog
stresa. Time se omogućava brži oporavak i brže uvođenje u reprodukciju, što se
odražava na povećanje proizvodnje mleka, smanjenje troškova osemenjavanja i
veći broj teladi.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 07 - 10. oktobar 2021",
title = "Digitalne tehnologije i reprodukcija krava",
pages = "43-54",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3333"
}

Fratrić, N., Vakanjac, S., Stojić, M.,& Gvozdić, D.. (2021). Digitalne tehnologije i reprodukcija krava. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 07 - 10. oktobar 2021
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 43-54.
https://hdl.handle.net/21.15107/rcub_veterinar_3333

Fratrić N, Vakanjac S, Stojić M, Gvozdić D. Digitalne tehnologije i reprodukcija krava. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 07 - 10. oktobar 2021. 2021;:43-54.
https://hdl.handle.net/21.15107/rcub_veterinar_3333 .

Fratrić, Natalija, Vakanjac, Slobodanka, Stojić, Milica, Gvozdić, Dragan, "Digitalne tehnologije i reprodukcija krava" in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 07 - 10. oktobar 2021 (2021):43-54,
https://hdl.handle.net/21.15107/rcub_veterinar_3333 .

Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge University Press., 87(4), 429-435.
https://hdl.handle.net/21.15107/rcub_veterinar_1976

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
https://hdl.handle.net/21.15107/rcub_veterinar_1976 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://hdl.handle.net/21.15107/rcub_veterinar_1976 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3334
AB  - Imunski sistem igra važnu ulogu u fertilizaciji, uspostavljanju i održavanju
graviditeta. Imunski mehanizmi semenika opremljeni su fizičkom i imunskom zaštitom antigena spermatozoida (autoantigena) od imunskog sistema domaćina
kao i mehanizmima za suzbijanje mikrobijalnih patogena. U pubertetu kada je
već uspostavljena imunska kompetentnost, germinativne – polne ćelije završavaju svoj prvi ciklus dok se diferentciraju u zrele spermatozoide. U ovom periodu
se sintetiše niz novih površinskih molekula koji se zajedno sa spermatozoidima u
razvoju (autoantigenima) “predstavljaju” imunskom sistemu. Prisustvo fizičke krv
– testis barijere čini da su testisi sisara imunološki privilegovano mesto koje štiti
autoantigene polnih ćelija od sistemskog imunskog odgovora. Lokalni imunomodulatori kao što su makrofagi, ćelije prirodne ubice (engl. natural killer, NK), dendritične ćelije, mastociti i T limfociti ne aktiviraju imunsku reakciju odbacivanja i
na taj način omogućavaju spermatozoidima da izbegnu napad imunskog sistema
(lokalna imunosupresivna sredina).
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
T1  - Imunologija sperme
SP  - 145
EP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3334
ER  - 

@conference{
author = "Fratrić, Natalija and Gvozdić, Dragan",
year = "2020",
abstract = "Imunski sistem igra važnu ulogu u fertilizaciji, uspostavljanju i održavanju
graviditeta. Imunski mehanizmi semenika opremljeni su fizičkom i imunskom zaštitom antigena spermatozoida (autoantigena) od imunskog sistema domaćina
kao i mehanizmima za suzbijanje mikrobijalnih patogena. U pubertetu kada je
već uspostavljena imunska kompetentnost, germinativne – polne ćelije završavaju svoj prvi ciklus dok se diferentciraju u zrele spermatozoide. U ovom periodu
se sintetiše niz novih površinskih molekula koji se zajedno sa spermatozoidima u
razvoju (autoantigenima) “predstavljaju” imunskom sistemu. Prisustvo fizičke krv
– testis barijere čini da su testisi sisara imunološki privilegovano mesto koje štiti
autoantigene polnih ćelija od sistemskog imunskog odgovora. Lokalni imunomodulatori kao što su makrofagi, ćelije prirodne ubice (engl. natural killer, NK), dendritične ćelije, mastociti i T limfociti ne aktiviraju imunsku reakciju odbacivanja i
na taj način omogućavaju spermatozoidima da izbegnu napad imunskog sistema
(lokalna imunosupresivna sredina).",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020",
title = "Imunologija sperme",
pages = "145-155",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3334"
}

Fratrić, N.,& Gvozdić, D.. (2020). Imunologija sperme. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 145-155.
https://hdl.handle.net/21.15107/rcub_veterinar_3334

Fratrić N, Gvozdić D. Imunologija sperme. in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020. 2020;:145-155.
https://hdl.handle.net/21.15107/rcub_veterinar_3334 .

Fratrić, Natalija, Gvozdić, Dragan, "Imunologija sperme" in 11. Naučni Simpozijum, Reprodukcija domaćih životinja, Divčibare, 8 - 11. oktobar 2020 (2020):145-155,
https://hdl.handle.net/21.15107/rcub_veterinar_3334 .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Stojić, Milica
AU  - Gvozdić, Dragan
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3336
AB  - Digitalne tehnologije današnjeg vremena nalaze svoje mesto u
svim sferama ljudskog rada, pa tako i u veterinarskoj medicini. Na
farmama mlečnih krava, one se koriste za praćenje parametara proizvodnje, ali i fizioloških parametara čime se doprinosi očuvanju zdravlja i dobrobiti životinja, kao i bezbednosti hrane. Digitalna tehnologija
najčešće podrazumeva upotrebu odgovarajućih mernih instrumenata
(senzora, kamera, mikrofona, skala i dr.) i analitičkih uređaja (specifičnih programskih paketa koji služe za obradu prikupljenih informacija) koji na različite načine transformišu dobijene podatke u informaciju
korisnu za držaoce životinja. Novim tehnologijama je omogućeno neprekidno praćenje brojnih parametara u realnom vremenu na određenim mernim mestima, prenos signala do centralnih jedinica i njihov
automatski unos u odgovarajuće programe. Tehnologije bazirane na
senzorima (biosenzorima), na farmama mlečnih krava omogućavaju
da se u realnom vremenu rukuje velikim brojem podataka iz različitih
sistema i na osnovu njih se mogu doneti važne odluke o upravljanu
farmom. Senzori mogu biti postavljeni na različitim mestima: u sistemu automatske muže, pojedinim lokacijama u objektima za držanje
životinja i na/u telu samih krava: ušima, vratu, nogama, repu, pod kožom ili u buragu. Podaci koji se generišu aktivnošću senzora, mogu
se koristiti za praćenje proizvodnih rezultata (mlečnost, broj somatskih
ćelija), uslova dobrobiti i adekvatnog smeštaja (spoljašnja temperatura, vlažnost vazduha), kao i fiziološkog stanja krava (detekcija estrusa). Dobrom obradom podataka se može, u određenim slučajevima,
predvideti buduće stanje životinja, što otvara mogućnost preventivnih
postupaka i aktivnosti veterinara. Primena digitalne tehnologije može značajno uticati na rad veterinara tako što će dovesti do brze transformacije sa terapijskog na preventivni pristup zaštiti zdravlja životinja. Sistem zasnovan na digitalnoj tehnologiji može da: 1) “signalizira”
veterinaru da kod određene životinje postoje promene u vrednostima
određenih homeostatskih parametara, 2) “predlaže” konkretne akcije veterinara u određenom vremenskom okviru, 3) “sugeriše” konkretne
uzroke nastanka promena, 4) integriše podatke iz više značajnih izvora i daje izveštaje, čime se dobijaju nove vrste podataka sa potpuno
novim značenjima.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLI Seminar za inovacije znanja veterinara, Beograd, 2020
T1  - Primena digitalne tehnologije na farmama mlečnih krava
SP  - 63
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3336
ER  - 

@conference{
author = "Fratrić, Natalija and Stojić, Milica and Gvozdić, Dragan",
year = "2020",
abstract = "Digitalne tehnologije današnjeg vremena nalaze svoje mesto u
svim sferama ljudskog rada, pa tako i u veterinarskoj medicini. Na
farmama mlečnih krava, one se koriste za praćenje parametara proizvodnje, ali i fizioloških parametara čime se doprinosi očuvanju zdravlja i dobrobiti životinja, kao i bezbednosti hrane. Digitalna tehnologija
najčešće podrazumeva upotrebu odgovarajućih mernih instrumenata
(senzora, kamera, mikrofona, skala i dr.) i analitičkih uređaja (specifičnih programskih paketa koji služe za obradu prikupljenih informacija) koji na različite načine transformišu dobijene podatke u informaciju
korisnu za držaoce životinja. Novim tehnologijama je omogućeno neprekidno praćenje brojnih parametara u realnom vremenu na određenim mernim mestima, prenos signala do centralnih jedinica i njihov
automatski unos u odgovarajuće programe. Tehnologije bazirane na
senzorima (biosenzorima), na farmama mlečnih krava omogućavaju
da se u realnom vremenu rukuje velikim brojem podataka iz različitih
sistema i na osnovu njih se mogu doneti važne odluke o upravljanu
farmom. Senzori mogu biti postavljeni na različitim mestima: u sistemu automatske muže, pojedinim lokacijama u objektima za držanje
životinja i na/u telu samih krava: ušima, vratu, nogama, repu, pod kožom ili u buragu. Podaci koji se generišu aktivnošću senzora, mogu
se koristiti za praćenje proizvodnih rezultata (mlečnost, broj somatskih
ćelija), uslova dobrobiti i adekvatnog smeštaja (spoljašnja temperatura, vlažnost vazduha), kao i fiziološkog stanja krava (detekcija estrusa). Dobrom obradom podataka se može, u određenim slučajevima,
predvideti buduće stanje životinja, što otvara mogućnost preventivnih
postupaka i aktivnosti veterinara. Primena digitalne tehnologije može značajno uticati na rad veterinara tako što će dovesti do brze transformacije sa terapijskog na preventivni pristup zaštiti zdravlja životinja. Sistem zasnovan na digitalnoj tehnologiji može da: 1) “signalizira”
veterinaru da kod određene životinje postoje promene u vrednostima
određenih homeostatskih parametara, 2) “predlaže” konkretne akcije veterinara u određenom vremenskom okviru, 3) “sugeriše” konkretne
uzroke nastanka promena, 4) integriše podatke iz više značajnih izvora i daje izveštaje, čime se dobijaju nove vrste podataka sa potpuno
novim značenjima.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLI Seminar za inovacije znanja veterinara, Beograd, 2020",
title = "Primena digitalne tehnologije na farmama mlečnih krava",
pages = "63-72",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3336"
}

Fratrić, N., Stojić, M.,& Gvozdić, D.. (2020). Primena digitalne tehnologije na farmama mlečnih krava. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 63-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3336

Fratrić N, Stojić M, Gvozdić D. Primena digitalne tehnologije na farmama mlečnih krava. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020. 2020;:63-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3336 .

Fratrić, Natalija, Stojić, Milica, Gvozdić, Dragan, "Primena digitalne tehnologije na farmama mlečnih krava" in XLI Seminar za inovacije znanja veterinara, Beograd, 2020 (2020):63-72,
https://hdl.handle.net/21.15107/rcub_veterinar_3336 .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1862
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
VL  - 133
SP  - 63
EP  - 74
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 

@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
volume = "133",
pages = "63-74",
doi = "10.1016/j.rvsc.2020.09.004"
}

Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004

Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004 .

Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 . .

TY  - CONF
AU  - Fratrić, Natalija
AU  - Stojić, Milica
AU  - Gvozdić, Dragan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3335
AB  - Imunitet mlečne žlezde zavisi od koordinisanog delovanja elemenata nespecifičnog i specifičnog imuniteta, što uključuje, kako anatomske karakteristike žlezde tako i ćelijske i humoralne odbrambene komponente. Primarne uloge imunskog sistema su da prevenira invaziju - naseljavanje mlečne žlezde bakterijama,
eliminiše postojeću infekciju i uspostavi normalnu funkciju tkiva mlečne žlezde.
Više spoljašnjih i unutrašnjih faktora može uticati na slabljenje imunskog odgovora domaćina, koji dodatno zavisi od virulentnosti i otpornosti različitih mikroorganizama, izazivača mastitisa. Stoga je poznavanje imuniteta mlečne žlezde, kako
u fiziološkim tako i u uslovima inflamacije, ključno za razumevanje prirode intramamarnih infekcija. Imunski odgovor mlečne žlezde varira u zavisnosti od perioda
laktacije mlečnih krava i suprimiran je kod stresa, oko perioda zasušenja i partusa
što povećava osetljivost mlečne žlezde na mastitise.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 10. Naučni simpozijum Reprodukcija domaćih životinja i bolesti mlečne žlezde, Divčibare, 10 - 13. oktobar 2019
T1  - Spečifičnosti imunskog odgovora mlečne žlezde i otpornost na mastite kod mlečnih krava
SP  - 181
SP  - 199
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3335
ER  - 

@conference{
author = "Fratrić, Natalija and Stojić, Milica and Gvozdić, Dragan",
year = "2019",
abstract = "Imunitet mlečne žlezde zavisi od koordinisanog delovanja elemenata nespecifičnog i specifičnog imuniteta, što uključuje, kako anatomske karakteristike žlezde tako i ćelijske i humoralne odbrambene komponente. Primarne uloge imunskog sistema su da prevenira invaziju - naseljavanje mlečne žlezde bakterijama,
eliminiše postojeću infekciju i uspostavi normalnu funkciju tkiva mlečne žlezde.
Više spoljašnjih i unutrašnjih faktora može uticati na slabljenje imunskog odgovora domaćina, koji dodatno zavisi od virulentnosti i otpornosti različitih mikroorganizama, izazivača mastitisa. Stoga je poznavanje imuniteta mlečne žlezde, kako
u fiziološkim tako i u uslovima inflamacije, ključno za razumevanje prirode intramamarnih infekcija. Imunski odgovor mlečne žlezde varira u zavisnosti od perioda
laktacije mlečnih krava i suprimiran je kod stresa, oko perioda zasušenja i partusa
što povećava osetljivost mlečne žlezde na mastitise.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "10. Naučni simpozijum Reprodukcija domaćih životinja i bolesti mlečne žlezde, Divčibare, 10 - 13. oktobar 2019",
title = "Spečifičnosti imunskog odgovora mlečne žlezde i otpornost na mastite kod mlečnih krava",
pages = "181-199",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3335"
}

Fratrić, N., Stojić, M.,& Gvozdić, D.. (2019). Spečifičnosti imunskog odgovora mlečne žlezde i otpornost na mastite kod mlečnih krava. in 10. Naučni simpozijum Reprodukcija domaćih životinja i bolesti mlečne žlezde, Divčibare, 10 - 13. oktobar 2019
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 181.
https://hdl.handle.net/21.15107/rcub_veterinar_3335

Fratrić N, Stojić M, Gvozdić D. Spečifičnosti imunskog odgovora mlečne žlezde i otpornost na mastite kod mlečnih krava. in 10. Naučni simpozijum Reprodukcija domaćih životinja i bolesti mlečne žlezde, Divčibare, 10 - 13. oktobar 2019. 2019;:181.
https://hdl.handle.net/21.15107/rcub_veterinar_3335 .

Fratrić, Natalija, Stojić, Milica, Gvozdić, Dragan, "Spečifičnosti imunskog odgovora mlečne žlezde i otpornost na mastite kod mlečnih krava" in 10. Naučni simpozijum Reprodukcija domaćih životinja i bolesti mlečne žlezde, Divčibare, 10 - 13. oktobar 2019 (2019):181,
https://hdl.handle.net/21.15107/rcub_veterinar_3335 .

TY  - CONF
AU  - Valčić, Olivera
AU  - Milanović, Svetlana
AU  - Fratrić, Natalija
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3412
AB  - U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Biohemijske karakteristike kolostruma
SP  - 112
EP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3412
ER  - 

@conference{
author = "Valčić, Olivera and Milanović, Svetlana and Fratrić, Natalija",
year = "2019",
abstract = "U radu su opisane osnovne grupe i biohemijske karakteristike organskih molekula koji su
od ključnog značaja za rast i razvoj novorođene prasadi. Među njima se posebno ističu laktoza,
koja ima ključnu ulogu u osmotskoj regulaciji količine izlučenog kolostruma i koja se sintetiše u
epitelnim ćelijama alveola mlečne žlezde. Zatim, masti koje su energetska komponenta čiji sastav
i količina zavise od niza faktora kao što su ishrana krmače i njen genetski potencijal. Prasad se
rađa agamaglobulinemična i neophodno je da u prvim časovima života posisaju kolostrum u
kojem su prisutna maternalna antitela (IgG) kako bi stekla imunitet neophodan za preživljavanje.
Pored navedenih sastojaka u kolostrumu se nalaze i visoke koncentracije minerala,
mikroelemenata, vitamina, hormona i enzima. Poseban osvrt u radu je dat dinamičnoj promeni
sastava i količine komponenti kolostruma od trenutka kada počinje da se luči do perioda tranzicije
u mleko. Istovremeno, objašnjeni su osnovni mehanizmi sinteze molekula u mlečnoj žlezdi i
transfera molekula iz krvne plazme svinje u kolostrum, kao i faktori koji utiču na sintezu ključnih
sastojaka kolostruma.",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Biohemijske karakteristike kolostruma",
pages = "112-121",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3412"
}

Valčić, O., Milanović, S.,& Fratrić, N.. (2019). Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412

Valčić O, Milanović S, Fratrić N. Biohemijske karakteristike kolostruma. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:112-121.
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

Valčić, Olivera, Milanović, Svetlana, Fratrić, Natalija, "Biohemijske karakteristike kolostruma" in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):112-121,
https://hdl.handle.net/21.15107/rcub_veterinar_3412 .

TY  - JOUR
AU  - Suluburić, Adam
AU  - Milanović, Svetlana
AU  - Vranješ-Đurić, Sanja
AU  - Jovanović, Ivan B.
AU  - Barna, Tomislav
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Szenci, Otto
AU  - Gvozdić, Dragan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1486
AB  - Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase
VL  - 65
IS  - 3
SP  - 446
EP  - 458
DO  - 10.1556/004.2017.042
ER  - 

@article{
author = "Suluburić, Adam and Milanović, Svetlana and Vranješ-Đurić, Sanja and Jovanović, Ivan B. and Barna, Tomislav and Stojić, Milica and Fratrić, Natalija and Szenci, Otto and Gvozdić, Dragan",
year = "2017",
abstract = "Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase",
volume = "65",
number = "3",
pages = "446-458",
doi = "10.1556/004.2017.042"
}

Suluburić, A., Milanović, S., Vranješ-Đurić, S., Jovanović, I. B., Barna, T., Stojić, M., Fratrić, N., Szenci, O.,& Gvozdić, D.. (2017). Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica
Akademiai Kiado Rt, Budapest., 65(3), 446-458.
https://doi.org/10.1556/004.2017.042

Suluburić A, Milanović S, Vranješ-Đurić S, Jovanović IB, Barna T, Stojić M, Fratrić N, Szenci O, Gvozdić D. Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica. 2017;65(3):446-458.
doi:10.1556/004.2017.042 .

Suluburić, Adam, Milanović, Svetlana, Vranješ-Đurić, Sanja, Jovanović, Ivan B., Barna, Tomislav, Stojić, Milica, Fratrić, Natalija, Szenci, Otto, Gvozdić, Dragan, "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase" in Acta Veterinaria Hungarica, 65, no. 3 (2017):446-458,
https://doi.org/10.1556/004.2017.042 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovacić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valčić, Olivera
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1519
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
VL  - 67
IS  - 4
SP  - 508
EP  - 524
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovacić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valčić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
volume = "67",
number = "4",
pages = "508-524",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovacić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valčić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovacić M, Ilić V, Gvozdić D, Savić O, Doković R, Valčić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovacić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valčić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Kovacić, Marijana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Obrenović, Sonja
AU  - Grujic-Milanović, Jelica
AU  - Arsić, Aleksandra
AU  - Milanović, Zorana
AU  - Savić, Olivera
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1478
AB  - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
VL  - 67
IS  - 2
SP  - 207
EP  - 227
DO  - 10.1515/acve-2017-0018
ER  - 

@article{
author = "Kovacić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujic-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna",
year = "2017",
abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers",
volume = "67",
number = "2",
pages = "207-227",
doi = "10.1515/acve-2017-0018"
}

Kovacić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujic-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227.
https://doi.org/10.1515/acve-2017-0018

Kovacić M, Marković D, Maslovarić I, Obrenović S, Grujic-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227.
doi:10.1515/acve-2017-0018 .

Kovacić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujic-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227,
https://doi.org/10.1515/acve-2017-0018 . .

TY  - JOUR
AU  - Đoković, Radojica
AU  - Dosković, Vladimir
AU  - Cincović, Marko
AU  - Belić, Branislava
AU  - Fratrić, Natalija
AU  - Jasović, Boban
AU  - Lalović, Miroslav
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1547
AB  - The objective of this study was to determine insulin resistance in healthy (n=8) and ketotic (n=7) dairy cows based on the difference between basal and dynamic changes in glucose, insulin, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB) concentrations, and on the determination of their mutual relationship and relationship with insulin resistance indices (RQUICKI and RQUICKI-BHB), after intravenous glucose tolerance test (IVGTT). The RQUICKI index showed a significant linear correlation (P  lt  0.05) with basal insulin, glucose, NEFA and BHB levels. However, RQUICKI-BHB index values exhibited a negative correlation (P  lt  0.01) with basal NEFA and BHB values and their clearance rates, as well as with glucose clearance rate, and a positive correlation (P  lt  0.05) with basal insulin values as well as with insulin and glucose responses during IVGTT. The correlation between basal values of these parameters and the values measured or calculated during IVGTT is the result of RQUICKI-BHB values, as the exclusion of RQUICKI-BHB leads to loss of the statistical significance of the correlations between basal and dynamic values. Insulin resistance in ketotic cows is characterized by decreased insulin response to glucose and increased insulin resistance of the tissue, their correlation being the result of the RQUICKI-BHB index value.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test
VL  - 37
IS  - 4
SP  - 387
EP  - 392
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1547
ER  - 

@article{
author = "Đoković, Radojica and Dosković, Vladimir and Cincović, Marko and Belić, Branislava and Fratrić, Natalija and Jasović, Boban and Lalović, Miroslav",
year = "2017",
abstract = "The objective of this study was to determine insulin resistance in healthy (n=8) and ketotic (n=7) dairy cows based on the difference between basal and dynamic changes in glucose, insulin, non-esterified fatty acids (NEFA), ß-hydroxybutyrate (BHB) concentrations, and on the determination of their mutual relationship and relationship with insulin resistance indices (RQUICKI and RQUICKI-BHB), after intravenous glucose tolerance test (IVGTT). The RQUICKI index showed a significant linear correlation (P  lt  0.05) with basal insulin, glucose, NEFA and BHB levels. However, RQUICKI-BHB index values exhibited a negative correlation (P  lt  0.01) with basal NEFA and BHB values and their clearance rates, as well as with glucose clearance rate, and a positive correlation (P  lt  0.05) with basal insulin values as well as with insulin and glucose responses during IVGTT. The correlation between basal values of these parameters and the values measured or calculated during IVGTT is the result of RQUICKI-BHB values, as the exclusion of RQUICKI-BHB leads to loss of the statistical significance of the correlations between basal and dynamic values. Insulin resistance in ketotic cows is characterized by decreased insulin response to glucose and increased insulin resistance of the tissue, their correlation being the result of the RQUICKI-BHB index value.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test",
volume = "37",
number = "4",
pages = "387-392",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1547"
}

Đoković, R., Dosković, V., Cincović, M., Belić, B., Fratrić, N., Jasović, B.,& Lalović, M.. (2017). Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 37(4), 387-392.
https://hdl.handle.net/21.15107/rcub_veterinar_1547

Đoković R, Dosković V, Cincović M, Belić B, Fratrić N, Jasović B, Lalović M. Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test. in Pakistan Veterinary Journal. 2017;37(4):387-392.
https://hdl.handle.net/21.15107/rcub_veterinar_1547 .

Đoković, Radojica, Dosković, Vladimir, Cincović, Marko, Belić, Branislava, Fratrić, Natalija, Jasović, Boban, Lalović, Miroslav, "Estimation of insulin resistance in healthy and ketotic cows during an intravenous glucose tolerance test" in Pakistan Veterinary Journal, 37, no. 4 (2017):387-392,
https://hdl.handle.net/21.15107/rcub_veterinar_1547 .

TY  - JOUR
AU  - Buac, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1403
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
VL  - 106
SP  - 135
EP  - 142
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Buac, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
volume = "106",
pages = "135-142",
doi = "10.1016/j.rvsc.2016.04.002"
}

Buac, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Buac M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Buac, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Gvozdić, Dragan
AU  - Fratrić, Natalija
AU  - Kirovski, Danijela
AU  - Đoković, Radojica
AU  - Sadojević, Zeljko
AU  - Cincović, Marko
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1176
AB  - The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ΔBCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids (NEFA), glucose, triacylglycerol (TAG) and total bilirubin (tBIL) in healthy dairy cows during transition period. Twenty healthy Holstein cows were included and categorized into groups based on BCS loss (ΔBCS) between dry period and early lactation (ΔBCS  lt 0.75 and ≥0.75). Significant differences between groups (p lt 0.05) were observed for blood serum NEFA, glucose and tBIL. Cows with high ΔBCS (≥0.75) between dry period and early lactation showed increased blood serum NEFA, TAG and tBIL concentrations and lower blood serum glucose concentration during transition period, compared to the low ΔBCS cows ( lt 0.75). Metabolic profiles of cows during transition period indicate high level of lipid mobilization from adipose tissue, possible decreased liver cells TAG export and gluconeogenic ability and impaired bilirubin metabolism if ΔBCS is increased by more than 0.75 points. ΔBCS was in relation with energy balance (EB) in transition period.
PB  - Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow
T2  - Animal Science Papers and Reports
T1  - Body condition score loss, hepatic lipidosis and selected blood metabolites in holstein cows during transition period
VL  - 33
IS  - 1
SP  - 35
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1176
ER  - 

@article{
author = "Šamanc, Horea and Gvozdić, Dragan and Fratrić, Natalija and Kirovski, Danijela and Đoković, Radojica and Sadojević, Zeljko and Cincović, Marko",
year = "2015",
abstract = "The aim of this study was to investigate the relationship between the loss in body condition score (BCS) and loss (ΔBCS), energy balance (EB), hepatic lipidosis and blood serum concentration of non esterified fatty acids (NEFA), glucose, triacylglycerol (TAG) and total bilirubin (tBIL) in healthy dairy cows during transition period. Twenty healthy Holstein cows were included and categorized into groups based on BCS loss (ΔBCS) between dry period and early lactation (ΔBCS  lt 0.75 and ≥0.75). Significant differences between groups (p lt 0.05) were observed for blood serum NEFA, glucose and tBIL. Cows with high ΔBCS (≥0.75) between dry period and early lactation showed increased blood serum NEFA, TAG and tBIL concentrations and lower blood serum glucose concentration during transition period, compared to the low ΔBCS cows ( lt 0.75). Metabolic profiles of cows during transition period indicate high level of lipid mobilization from adipose tissue, possible decreased liver cells TAG export and gluconeogenic ability and impaired bilirubin metabolism if ΔBCS is increased by more than 0.75 points. ΔBCS was in relation with energy balance (EB) in transition period.",
publisher = "Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow",
journal = "Animal Science Papers and Reports",
title = "Body condition score loss, hepatic lipidosis and selected blood metabolites in holstein cows during transition period",
volume = "33",
number = "1",
pages = "35-47",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1176"
}

Šamanc, H., Gvozdić, D., Fratrić, N., Kirovski, D., Đoković, R., Sadojević, Z.,& Cincović, M.. (2015). Body condition score loss, hepatic lipidosis and selected blood metabolites in holstein cows during transition period. in Animal Science Papers and Reports
Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow., 33(1), 35-47.
https://hdl.handle.net/21.15107/rcub_veterinar_1176

Šamanc H, Gvozdić D, Fratrić N, Kirovski D, Đoković R, Sadojević Z, Cincović M. Body condition score loss, hepatic lipidosis and selected blood metabolites in holstein cows during transition period. in Animal Science Papers and Reports. 2015;33(1):35-47.
https://hdl.handle.net/21.15107/rcub_veterinar_1176 .

Šamanc, Horea, Gvozdić, Dragan, Fratrić, Natalija, Kirovski, Danijela, Đoković, Radojica, Sadojević, Zeljko, Cincović, Marko, "Body condition score loss, hepatic lipidosis and selected blood metabolites in holstein cows during transition period" in Animal Science Papers and Reports, 33, no. 1 (2015):35-47,
https://hdl.handle.net/21.15107/rcub_veterinar_1176 .

TY  - JOUR
AU  - Đoković, Radojica
AU  - Kurcubić, Vladimir
AU  - Ilić, Zoran
AU  - Cincović, Marko
AU  - Petrović, Milun D.
AU  - Fratrić, Natalija
AU  - Jasović, Boban
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/955
AB  - The objective of this experiment was to evaluate the metabolic status of late pregnant and early lactation dairy cows based on changes in characteristic blood metabolites. Blood samples were collected from 15 late pregnant cows and 15 early lactation cows to measure beta-hydroxybutyrate (BHB), non-esterifified fatty acids (NEFA), triglycerides (TG), glucose, total protein (TP), albumin, total bilirubin, urea and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase (GGT). Cows in early lactation had signififi cantly higher (P lt 0.05) levels of serum BHB and NEFA, and lower (P lt 0.05) glycaemia compared to the late pregnant cows. High lipomobilization (NEFA>0.4 mmol/L) was detected in 6 (40%) of early lactation cows but in none of the late pregnant cows, while subclinical ketosis (BHB>1.2 mmol/L) was detected in 14 (94.4%) of the early lactation cows and 4 (26.6%) of the late pregnant cows. AST activities above 100 U/L were detected in 2 early lactation cows and in none of the late pregnant cows. TG levels below 0.12 mmol/L and glucose below 2.5 mmol/L were found in 7 (44%) and 10 (66.6%) of the early lactation cows, respectively, and in none of the late pregnant cows. Early lactation cows were found to have lower blood serum levels of TG (P lt 0.05), albumin (P lt 0.05) urea (P lt 0.05) and GTT (P>0.05) activities and higher concentrations of total bilirubin (P>0.05) TP (P>0.05) and AST activities (P lt 0.05) compared to the late pregnant cows. The results of blood serum levels of glucose, TG, BHB, NEFA and AST in early lactation cows suggest metabolic disorders associated with ketosis, and some degree of hepatic lesions, probably due to fat infifiltration. These serum parameters may have a key role in evaluating metabolic status in late pregnant and early lactation dairy cows.
PB  - Univ Zagreb Vet Faculty, Zagreb
T2  - Veterinarski Arhiv
T1  - Evaluation of metabolic status in Simmental dairy cows during late pregnancy and early lactation
T1  - Prosudba metaboličkog statusa mliječnih krava simentalske pasmine tijekom kasne gravidnosti i na početku laktacije
VL  - 83
IS  - 6
SP  - 593
EP  - 602
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_955
ER  - 

@article{
author = "Đoković, Radojica and Kurcubić, Vladimir and Ilić, Zoran and Cincović, Marko and Petrović, Milun D. and Fratrić, Natalija and Jasović, Boban",
year = "2013",
abstract = "The objective of this experiment was to evaluate the metabolic status of late pregnant and early lactation dairy cows based on changes in characteristic blood metabolites. Blood samples were collected from 15 late pregnant cows and 15 early lactation cows to measure beta-hydroxybutyrate (BHB), non-esterifified fatty acids (NEFA), triglycerides (TG), glucose, total protein (TP), albumin, total bilirubin, urea and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase (GGT). Cows in early lactation had signififi cantly higher (P lt 0.05) levels of serum BHB and NEFA, and lower (P lt 0.05) glycaemia compared to the late pregnant cows. High lipomobilization (NEFA>0.4 mmol/L) was detected in 6 (40%) of early lactation cows but in none of the late pregnant cows, while subclinical ketosis (BHB>1.2 mmol/L) was detected in 14 (94.4%) of the early lactation cows and 4 (26.6%) of the late pregnant cows. AST activities above 100 U/L were detected in 2 early lactation cows and in none of the late pregnant cows. TG levels below 0.12 mmol/L and glucose below 2.5 mmol/L were found in 7 (44%) and 10 (66.6%) of the early lactation cows, respectively, and in none of the late pregnant cows. Early lactation cows were found to have lower blood serum levels of TG (P lt 0.05), albumin (P lt 0.05) urea (P lt 0.05) and GTT (P>0.05) activities and higher concentrations of total bilirubin (P>0.05) TP (P>0.05) and AST activities (P lt 0.05) compared to the late pregnant cows. The results of blood serum levels of glucose, TG, BHB, NEFA and AST in early lactation cows suggest metabolic disorders associated with ketosis, and some degree of hepatic lesions, probably due to fat infifiltration. These serum parameters may have a key role in evaluating metabolic status in late pregnant and early lactation dairy cows.",
publisher = "Univ Zagreb Vet Faculty, Zagreb",
journal = "Veterinarski Arhiv",
title = "Evaluation of metabolic status in Simmental dairy cows during late pregnancy and early lactation, Prosudba metaboličkog statusa mliječnih krava simentalske pasmine tijekom kasne gravidnosti i na početku laktacije",
volume = "83",
number = "6",
pages = "593-602",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_955"
}

Đoković, R., Kurcubić, V., Ilić, Z., Cincović, M., Petrović, M. D., Fratrić, N.,& Jasović, B.. (2013). Evaluation of metabolic status in Simmental dairy cows during late pregnancy and early lactation. in Veterinarski Arhiv
Univ Zagreb Vet Faculty, Zagreb., 83(6), 593-602.
https://hdl.handle.net/21.15107/rcub_veterinar_955

Đoković R, Kurcubić V, Ilić Z, Cincović M, Petrović MD, Fratrić N, Jasović B. Evaluation of metabolic status in Simmental dairy cows during late pregnancy and early lactation. in Veterinarski Arhiv. 2013;83(6):593-602.
https://hdl.handle.net/21.15107/rcub_veterinar_955 .

Đoković, Radojica, Kurcubić, Vladimir, Ilić, Zoran, Cincović, Marko, Petrović, Milun D., Fratrić, Natalija, Jasović, Boban, "Evaluation of metabolic status in Simmental dairy cows during late pregnancy and early lactation" in Veterinarski Arhiv, 83, no. 6 (2013):593-602,
https://hdl.handle.net/21.15107/rcub_veterinar_955 .

TY  - JOUR
AU  - Đoković, Radojica
AU  - Kurcubić, Vladimir
AU  - Ilić, Zoran
AU  - Cincović, Marko
AU  - Fratrić, Natalija
AU  - Stanimirović, Zoran
AU  - Petrović, Milun D.
AU  - Petrović, Milan P.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1022
AB  - Fifteen early-lactation cows and 15 mid-lactation cows were chosen for the analysis. Blood samples were collected to measure the beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), glucose (Glu), total protein (TP), albumin (ALB), total bilirubin (TB), urea (U) and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase (GGT). Earlylactation cows showed significantly higher (P lt 0.05) values of serum BHB and NEFA, and lower of (P lt 0.05) glycemia levels compared to mid-lactation cows. High lipomobilization (NEFA >0.4 mmol/l) and subclinical ketosis (BHB >1.2 mmol/L) were detected in 6 (40%) and 14 (94.4%) early-lactation cows, respectively, and in none of the mid lactation cows. AST activities above 100 IU/l were detected in two early-lactation and none of the mid-lactation cows. TG concentrations below 0.12 mmol/l were found in 7 (44%) early-lactation and 2 (13.3%) mid-lactation cows. Glucose levels were below 2.5 mmol/l in 10 (66.6%) early-lactation and 5 (33.3%) mid-lactation cows. Early-lactation cows showed lower blood serum concentrations of TG (P>0.05), ALB (P>0.05), TP (P lt 0.05), U (P>0.05) and GTT (P>0.05) activities and higher concentrations of TB (P>0.05) and AST activities (P lt 0.05), as compared to mid-lactation cows. These metabolic characteristics were correlated with DMI and energy balance (EB). Blood serum values for glucose, TG, BHB, NEFA and AST showed that earlylactation cows suffered from metabolic disturbances, associated with ketosis, and some degree of hepatic lesions, probably due to fat infiltration. These serum parameters may have a key role in evaluating the metabolic status of dairy cows.
PB  - Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow
T2  - Animal Science Papers and Reports
T1  - Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation
VL  - 31
IS  - 2
SP  - 101
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1022
ER  - 

@article{
author = "Đoković, Radojica and Kurcubić, Vladimir and Ilić, Zoran and Cincović, Marko and Fratrić, Natalija and Stanimirović, Zoran and Petrović, Milun D. and Petrović, Milan P.",
year = "2013",
abstract = "Fifteen early-lactation cows and 15 mid-lactation cows were chosen for the analysis. Blood samples were collected to measure the beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), glucose (Glu), total protein (TP), albumin (ALB), total bilirubin (TB), urea (U) and the activity of aspartate transaminase (AST) and gamma-glutamyl transferase (GGT). Earlylactation cows showed significantly higher (P lt 0.05) values of serum BHB and NEFA, and lower of (P lt 0.05) glycemia levels compared to mid-lactation cows. High lipomobilization (NEFA >0.4 mmol/l) and subclinical ketosis (BHB >1.2 mmol/L) were detected in 6 (40%) and 14 (94.4%) early-lactation cows, respectively, and in none of the mid lactation cows. AST activities above 100 IU/l were detected in two early-lactation and none of the mid-lactation cows. TG concentrations below 0.12 mmol/l were found in 7 (44%) early-lactation and 2 (13.3%) mid-lactation cows. Glucose levels were below 2.5 mmol/l in 10 (66.6%) early-lactation and 5 (33.3%) mid-lactation cows. Early-lactation cows showed lower blood serum concentrations of TG (P>0.05), ALB (P>0.05), TP (P lt 0.05), U (P>0.05) and GTT (P>0.05) activities and higher concentrations of TB (P>0.05) and AST activities (P lt 0.05), as compared to mid-lactation cows. These metabolic characteristics were correlated with DMI and energy balance (EB). Blood serum values for glucose, TG, BHB, NEFA and AST showed that earlylactation cows suffered from metabolic disturbances, associated with ketosis, and some degree of hepatic lesions, probably due to fat infiltration. These serum parameters may have a key role in evaluating the metabolic status of dairy cows.",
publisher = "Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow",
journal = "Animal Science Papers and Reports",
title = "Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation",
volume = "31",
number = "2",
pages = "101-110",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1022"
}

Đoković, R., Kurcubić, V., Ilić, Z., Cincović, M., Fratrić, N., Stanimirović, Z., Petrović, M. D.,& Petrović, M. P.. (2013). Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation. in Animal Science Papers and Reports
Polska Akad Nauk, Inst Genetyki I Hodowli Zwierzat, Mrokow., 31(2), 101-110.
https://hdl.handle.net/21.15107/rcub_veterinar_1022

Đoković R, Kurcubić V, Ilić Z, Cincović M, Fratrić N, Stanimirović Z, Petrović MD, Petrović MP. Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation. in Animal Science Papers and Reports. 2013;31(2):101-110.
https://hdl.handle.net/21.15107/rcub_veterinar_1022 .

Đoković, Radojica, Kurcubić, Vladimir, Ilić, Zoran, Cincović, Marko, Fratrić, Natalija, Stanimirović, Zoran, Petrović, Milun D., Petrović, Milan P., "Evaluation of the metabolic status of Simmental dairy cows in early and mid lactation" in Animal Science Papers and Reports, 31, no. 2 (2013):101-110,
https://hdl.handle.net/21.15107/rcub_veterinar_1022 .

TY  - JOUR
AU  - Đoković, Radojica
AU  - Šamanc, Horea
AU  - Jovanović, Milijan
AU  - Fratrić, Natalija
AU  - Dosković, Vladimir
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1046
AB  - Background: Production diseases, such as those associated with improper nutrition or management are common in transitional high-yielding dairy cows. The diseases listed in this include: the fat liver syndrome, ketosis, oxidative stress, laminitis, mastitis, milk fever, retained placenta, metritis and infertility. The diseases occur mainly around calving. They are all interrelated and form the so-called periparturient disease complex. The term transition is to underscore the important physiological, metabolic, nutritional and endocrine changes occurring in this time frame. Diagnosing liver lipidosis and susceptibility of ketosis in dairy cows may include liver biopsy or ecography, but a less invasive and more economical analytical method may be the measurement of blood biochemical metabolites. The objective of the present study was to determine a relationship among blood indicators of hepatic function and lipid content in the liver during transitional period in high-yielding dairy cows. Materials, Methods & Results: Late pregnant and calved cows (n = 40) were selected from a Holstein dairy herd and allocated to four groups: a late pregnant cows (n = 10) from day 15 to day 5 before calving; late pregnant cows (n = 10) from day 4 to day 1 before calving; clinically puerperal healthy cows (n = 10) and clinically ketotic puerperal cows (n = 10). Liver and blood samples were taken from all cows. The blood metabolites concentrations were determined by photometric methods using a Cobas Mira automatic analyzer. Liver specimens were histopathologically analyzed for lipid contents using a freezing microtome. The statistical analysis of the obtained data was carried out by ANOVA-procedure. The results of present investigation have shown that the lipid content in the liver and the blood non-esterificed fatty acids, beta-hydroxybutyrate, total bilirubin concentrations and the AST activities were significantly higher (P < 0.05) as well the blood glucose, total cholesterol, triglycerides and albumin concentrations were significantly lower (P < 0.05) in puerperal ketotic cows as compared to the values of these parameters in the blood of healthy cows in the transition period. Discussion: Liver biopsy is the only reliable method to determine severity of fatty liver in dairy cattle in the transitional period. Blood, urine and milk metabolites or blood enzyme activity have been proposed as diagnostic tools. This investigation demonstrated that in healthy transitional cows a mild fatty infiltration occurred in liver during the late pregnancy and early lactation. The histopathological examination showed a moderate to severe degree of fatty liver in ketotic cows. The lipomobilisation markers, serum BHB and NEFA concentrations, were markedly enhanced in puerperal ketotic cows. However, liver steatosis compromised hepatocyte metabolism, leading to significantly weaker circulating concentrations of glucose, TG and total cholesterol, and induced some cellular lesions as evidenced by significant increases in the serum bilirubin concentrations and in the AST enzyme activities in puerperal ketotic cows All these biochemical metabolites may be used as important biochemical indicators in the determination of the functional status of the liver in high-yielding dairy cows during the transition period.
PB  - Univ Fed Rio Grande Do Sul, Porto Alegre Rs
T2  - Acta Scientiae Veterinariae
T1  - Relationship among Blood Indicators of Hepatic Function and Lipid Content in the Liver during Transitional Period in High-Yielding Dairy Cows
VL  - 41
SP  - 1128
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1046
ER  - 

@article{
author = "Đoković, Radojica and Šamanc, Horea and Jovanović, Milijan and Fratrić, Natalija and Dosković, Vladimir and Stanimirović, Zoran",
year = "2013",
abstract = "Background: Production diseases, such as those associated with improper nutrition or management are common in transitional high-yielding dairy cows. The diseases listed in this include: the fat liver syndrome, ketosis, oxidative stress, laminitis, mastitis, milk fever, retained placenta, metritis and infertility. The diseases occur mainly around calving. They are all interrelated and form the so-called periparturient disease complex. The term transition is to underscore the important physiological, metabolic, nutritional and endocrine changes occurring in this time frame. Diagnosing liver lipidosis and susceptibility of ketosis in dairy cows may include liver biopsy or ecography, but a less invasive and more economical analytical method may be the measurement of blood biochemical metabolites. The objective of the present study was to determine a relationship among blood indicators of hepatic function and lipid content in the liver during transitional period in high-yielding dairy cows. Materials, Methods & Results: Late pregnant and calved cows (n = 40) were selected from a Holstein dairy herd and allocated to four groups: a late pregnant cows (n = 10) from day 15 to day 5 before calving; late pregnant cows (n = 10) from day 4 to day 1 before calving; clinically puerperal healthy cows (n = 10) and clinically ketotic puerperal cows (n = 10). Liver and blood samples were taken from all cows. The blood metabolites concentrations were determined by photometric methods using a Cobas Mira automatic analyzer. Liver specimens were histopathologically analyzed for lipid contents using a freezing microtome. The statistical analysis of the obtained data was carried out by ANOVA-procedure. The results of present investigation have shown that the lipid content in the liver and the blood non-esterificed fatty acids, beta-hydroxybutyrate, total bilirubin concentrations and the AST activities were significantly higher (P < 0.05) as well the blood glucose, total cholesterol, triglycerides and albumin concentrations were significantly lower (P < 0.05) in puerperal ketotic cows as compared to the values of these parameters in the blood of healthy cows in the transition period. Discussion: Liver biopsy is the only reliable method to determine severity of fatty liver in dairy cattle in the transitional period. Blood, urine and milk metabolites or blood enzyme activity have been proposed as diagnostic tools. This investigation demonstrated that in healthy transitional cows a mild fatty infiltration occurred in liver during the late pregnancy and early lactation. The histopathological examination showed a moderate to severe degree of fatty liver in ketotic cows. The lipomobilisation markers, serum BHB and NEFA concentrations, were markedly enhanced in puerperal ketotic cows. However, liver steatosis compromised hepatocyte metabolism, leading to significantly weaker circulating concentrations of glucose, TG and total cholesterol, and induced some cellular lesions as evidenced by significant increases in the serum bilirubin concentrations and in the AST enzyme activities in puerperal ketotic cows All these biochemical metabolites may be used as important biochemical indicators in the determination of the functional status of the liver in high-yielding dairy cows during the transition period.",
publisher = "Univ Fed Rio Grande Do Sul, Porto Alegre Rs",
journal = "Acta Scientiae Veterinariae",
title = "Relationship among Blood Indicators of Hepatic Function and Lipid Content in the Liver during Transitional Period in High-Yielding Dairy Cows",
volume = "41",
pages = "1128",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1046"
}

Đoković, R., Šamanc, H., Jovanović, M., Fratrić, N., Dosković, V.,& Stanimirović, Z.. (2013). Relationship among Blood Indicators of Hepatic Function and Lipid Content in the Liver during Transitional Period in High-Yielding Dairy Cows. in Acta Scientiae Veterinariae
Univ Fed Rio Grande Do Sul, Porto Alegre Rs., 41, 1128.
https://hdl.handle.net/21.15107/rcub_veterinar_1046

Đoković R, Šamanc H, Jovanović M, Fratrić N, Dosković V, Stanimirović Z. Relationship among Blood Indicators of Hepatic Function and Lipid Content in the Liver during Transitional Period in High-Yielding Dairy Cows. in Acta Scientiae Veterinariae. 2013;41:1128.
https://hdl.handle.net/21.15107/rcub_veterinar_1046 .

Đoković, Radojica, Šamanc, Horea, Jovanović, Milijan, Fratrić, Natalija, Dosković, Vladimir, Stanimirović, Zoran, "Relationship among Blood Indicators of Hepatic Function and Lipid Content in the Liver during Transitional Period in High-Yielding Dairy Cows" in Acta Scientiae Veterinariae, 41 (2013):1128,
https://hdl.handle.net/21.15107/rcub_veterinar_1046 .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Stojić, Milica
AU  - Đorić, Goran
AU  - Petrović, M.
AU  - Đoković, R.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1048
AB  - The objective of this study was to determine the levels of insulin, insulin like growth factor I (IGF-I) and thyroid hormones in relation to the body condition score (BCS) of periparturient dairy cows. The study was carried out on twenty Holstein-Friesian dairy cows with average milk production of 7000 L/305 days in the previous lactation, parity ranging from 2-4. All cows were BCS scored during the early dry period, 7±3 days before and after parturition. Based on the BCS at the early dry period, cows were divided in two groups: cows with high BCS (3.75- 4.25, HBCS, n=10), and cows with moderate BCS (2.75-3.75, MBCS, n=10). Blood samples were taken at the time of BCS evaluation. Concentrations of insulin, IGF-I, triiodothyroinine (T3) and thyroxine (T4) were determined by radioimmunoassay (RIA, INEP-Zemun, Serbia). Statistical differences between mean values were determined using Student t-test (p lt 0.01). Mean BCS values at early dry period were significantly different compared to two other periods (p lt 0.01) within both groups, but BCS was similar in both groups at 7±3 days after calving (2.69±0.67:2.62± 0.27, p>0.05). IGF-I level in HBCS cows at days 7±3 before calving was significantly higher (16.28±3.07:11.76±2.28, p lt 0.01), with a reverse relationship after calving (3.77±1.64:8.46±2.37, p lt 0.01). Insulin level was significantly lower at 7±3 days before calving in HBCS cows (16.26±4.60:20.18±4.96mIU/L, p lt 0.05). Thyroid hormones levels were significantly lower in HBCS group et all examined periods.
AB  - Cilj ovog rada je bio da se odredi nivo insulina, insulinu sličnog faktora rasta IGF-I, trijodtironina (T3) i tiroksina (T4) u krvi u odnosu na promene telesne kondicije kod krava u peripartalnom periodu. Za ogled je odabrano 20 krava holštajn - frizijske rase između 2-4 laktacije i čija je proizvodnja mleka u predhodnoj laktaciji iznosila 7000 L za 305 dana. Telesna kondicija (OTK) je kod svih krava određivana na početku zasušenja, 7±3 dana pre i 7±3 posle teljenja. Na osnovu OTK na početku zasušenja, krave su bile podeljene u dve grupe: krave sa visokom OTK (3,75-4,25, n=10) i krave sa optimalnom OTK (2,75-3,75, n=10). Uzorci krvi su prikupljani u vreme određivanja OTK. Koncentracija insulina, IGF-I, trijodtironina (T3) i tiroksina (T4) određivana je RIA metodom (INEP-Zemun, Serbia). Statistička značajnost razlika između srednjih vrednosti procenjivana je Studentovim ttestom. Srednje vrednosti OTK u ranom periodu zasušenja bile su statistički značajno različite u odnosu na druga dva perioda (p lt 0,01) kod obe grupe, ali je OTK kod obe grupe bila slična 7±3 posle teljenja (2,69±0,67:2,62±0,27, p>0,05). Koncentracija IGF-I bila je statistički značajno viša kod krava sa visokom OTK, 7±3 dana pre teljenja (16,28±3,07:11,76±2,28, p lt 0,01), sa obrnutim odnosom 7±3 dana posle teljenja (3,77±1,64:8,46±2,37, p lt 0,01). Koncentracija insulina je bila značajno niža 7±3 dana pre teljenja kod krava sa visokom OTK (16,26±4,60:20,18±4,96 mIU/L, p lt 0,05). Nivo tireoidnih hormona je bio značajno niži kod krava sa visokom OTK u svim ispitivanim periodima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Levels of insulin, insulin-like growth factor-I and thyroid hormones in relation to the body condition score changes in periparturient dairy cows
T1  - Nivo insulina, insulinu sličnog faktora rasta-I i tireoidnih hormona u odnosu na promene telesne kondicije (OTK) u peripartalnom periodu kod krava
VL  - 63
IS  - 4
SP  - 385
EP  - 396
DO  - 10.2298/AVB1304385F
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Stojić, Milica and Đorić, Goran and Petrović, M. and Đoković, R.",
year = "2013",
abstract = "The objective of this study was to determine the levels of insulin, insulin like growth factor I (IGF-I) and thyroid hormones in relation to the body condition score (BCS) of periparturient dairy cows. The study was carried out on twenty Holstein-Friesian dairy cows with average milk production of 7000 L/305 days in the previous lactation, parity ranging from 2-4. All cows were BCS scored during the early dry period, 7±3 days before and after parturition. Based on the BCS at the early dry period, cows were divided in two groups: cows with high BCS (3.75- 4.25, HBCS, n=10), and cows with moderate BCS (2.75-3.75, MBCS, n=10). Blood samples were taken at the time of BCS evaluation. Concentrations of insulin, IGF-I, triiodothyroinine (T3) and thyroxine (T4) were determined by radioimmunoassay (RIA, INEP-Zemun, Serbia). Statistical differences between mean values were determined using Student t-test (p lt 0.01). Mean BCS values at early dry period were significantly different compared to two other periods (p lt 0.01) within both groups, but BCS was similar in both groups at 7±3 days after calving (2.69±0.67:2.62± 0.27, p>0.05). IGF-I level in HBCS cows at days 7±3 before calving was significantly higher (16.28±3.07:11.76±2.28, p lt 0.01), with a reverse relationship after calving (3.77±1.64:8.46±2.37, p lt 0.01). Insulin level was significantly lower at 7±3 days before calving in HBCS cows (16.26±4.60:20.18±4.96mIU/L, p lt 0.05). Thyroid hormones levels were significantly lower in HBCS group et all examined periods., Cilj ovog rada je bio da se odredi nivo insulina, insulinu sličnog faktora rasta IGF-I, trijodtironina (T3) i tiroksina (T4) u krvi u odnosu na promene telesne kondicije kod krava u peripartalnom periodu. Za ogled je odabrano 20 krava holštajn - frizijske rase između 2-4 laktacije i čija je proizvodnja mleka u predhodnoj laktaciji iznosila 7000 L za 305 dana. Telesna kondicija (OTK) je kod svih krava određivana na početku zasušenja, 7±3 dana pre i 7±3 posle teljenja. Na osnovu OTK na početku zasušenja, krave su bile podeljene u dve grupe: krave sa visokom OTK (3,75-4,25, n=10) i krave sa optimalnom OTK (2,75-3,75, n=10). Uzorci krvi su prikupljani u vreme određivanja OTK. Koncentracija insulina, IGF-I, trijodtironina (T3) i tiroksina (T4) određivana je RIA metodom (INEP-Zemun, Serbia). Statistička značajnost razlika između srednjih vrednosti procenjivana je Studentovim ttestom. Srednje vrednosti OTK u ranom periodu zasušenja bile su statistički značajno različite u odnosu na druga dva perioda (p lt 0,01) kod obe grupe, ali je OTK kod obe grupe bila slična 7±3 posle teljenja (2,69±0,67:2,62±0,27, p>0,05). Koncentracija IGF-I bila je statistički značajno viša kod krava sa visokom OTK, 7±3 dana pre teljenja (16,28±3,07:11,76±2,28, p lt 0,01), sa obrnutim odnosom 7±3 dana posle teljenja (3,77±1,64:8,46±2,37, p lt 0,01). Koncentracija insulina je bila značajno niža 7±3 dana pre teljenja kod krava sa visokom OTK (16,26±4,60:20,18±4,96 mIU/L, p lt 0,05). Nivo tireoidnih hormona je bio značajno niži kod krava sa visokom OTK u svim ispitivanim periodima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Levels of insulin, insulin-like growth factor-I and thyroid hormones in relation to the body condition score changes in periparturient dairy cows, Nivo insulina, insulinu sličnog faktora rasta-I i tireoidnih hormona u odnosu na promene telesne kondicije (OTK) u peripartalnom periodu kod krava",
volume = "63",
number = "4",
pages = "385-396",
doi = "10.2298/AVB1304385F"
}

Fratrić, N., Gvozdić, D., Stojić, M., Đorić, G., Petrović, M.,& Đoković, R.. (2013). Levels of insulin, insulin-like growth factor-I and thyroid hormones in relation to the body condition score changes in periparturient dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(4), 385-396.
https://doi.org/10.2298/AVB1304385F

Fratrić N, Gvozdić D, Stojić M, Đorić G, Petrović M, Đoković R. Levels of insulin, insulin-like growth factor-I and thyroid hormones in relation to the body condition score changes in periparturient dairy cows. in Acta Veterinaria-Beograd. 2013;63(4):385-396.
doi:10.2298/AVB1304385F .

Fratrić, Natalija, Gvozdić, Dragan, Stojić, Milica, Đorić, Goran, Petrović, M., Đoković, R., "Levels of insulin, insulin-like growth factor-I and thyroid hormones in relation to the body condition score changes in periparturient dairy cows" in Acta Veterinaria-Beograd, 63, no. 4 (2013):385-396,
https://doi.org/10.2298/AVB1304385F . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Buac, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/933
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology and Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG
VL  - 150
IS  - 3-4
SP  - 161
EP  - 168
DO  - 10.1016/j.vetimm.2012.09.009
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Buac, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology and Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG",
volume = "150",
number = "3-4",
pages = "161-168",
doi = "10.1016/j.vetimm.2012.09.009"
}

Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Buac, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG. in Veterinary Immunology and Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009

Fratrić N, Gvozdić D, Vuković D, Savić O, Buac M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG. in Veterinary Immunology and Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009 .

Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Buac, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes  IgG" in Veterinary Immunology and Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 . .