TY  - JOUR
AU  - Obadia, Thomas
AU  - Gutierrez-Bugallo, Gladys
AU  - Duong, Veasna
AU  - Nuñez, Ana I.
AU  - Fernandes, Rosilainy S.
AU  - Kamgang, Basile
AU  - Hery, Liza
AU  - Gomard, Yann
AU  - Abbo, Sandra R.
AU  - Jiolle, Davy
AU  - Glavinić, Uroš
AU  - Dupont-Rouzeyrol, Myrielle
AU  - Atyame, Célestine M.
AU  - Pocquet, Nicolas
AU  - Boyer, Sébastien
AU  - Dauga, Catherine
AU  - Vazeille, Marie
AU  - Yébakima, André
AU  - White, Michael T.
AU  - Koenraadt, Constantianus J. M.
AU  - Mavingui, Patrick
AU  - Vega-Rua, Anubis
AU  - Veronesi, Eva
AU  - Pijlman, Gorben P.
AU  - Paupy, Christophe
AU  - Busquets, Núria
AU  - Lourenço-de-Oliveira, Ricardo
AU  - De Lamballerie, Xavier
AU  - Failloux, Anna-Bella
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2505
AB  - First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus, both colonizing a large strip encompassing tropical and temperate regions. As part of the international project ZIKAlliance initiated in 2016, 50 mosquito populations from six species collected in 12 countries were experimentally infected with different Zika viruses. Here, we show that Ae. aegypti is mainly responsible for Zika virus transmission having the highest susceptibility to viral infections. Other species play a secondary role in transmission while Culex mosquitoes are largely non-susceptible. Zika strain is expected to significantly modulate transmission efficiency with African strains being more likely to cause an outbreak. As the distribution of Ae. aegypti will doubtless expand with climate change and without new marketed vaccines, all the ingredients are in place to relive a new pandemic of Zika.
PB  - Nature Research
T2  - Nature Communications
T1  - Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project
VL  - 13
IS  - 1
SP  - 4490
DO  - 10.1038/s41467-022-32234-y
ER  - 

@article{
author = "Obadia, Thomas and Gutierrez-Bugallo, Gladys and Duong, Veasna and Nuñez, Ana I. and Fernandes, Rosilainy S. and Kamgang, Basile and Hery, Liza and Gomard, Yann and Abbo, Sandra R. and Jiolle, Davy and Glavinić, Uroš and Dupont-Rouzeyrol, Myrielle and Atyame, Célestine M. and Pocquet, Nicolas and Boyer, Sébastien and Dauga, Catherine and Vazeille, Marie and Yébakima, André and White, Michael T. and Koenraadt, Constantianus J. M. and Mavingui, Patrick and Vega-Rua, Anubis and Veronesi, Eva and Pijlman, Gorben P. and Paupy, Christophe and Busquets, Núria and Lourenço-de-Oliveira, Ricardo and De Lamballerie, Xavier and Failloux, Anna-Bella",
year = "2022",
abstract = "First identified in 1947, Zika virus took roughly 70 years to cause a pandemic unusually associated with virus-induced brain damage in newborns. Zika virus is transmitted by mosquitoes, mainly Aedes aegypti, and secondarily, Aedes albopictus, both colonizing a large strip encompassing tropical and temperate regions. As part of the international project ZIKAlliance initiated in 2016, 50 mosquito populations from six species collected in 12 countries were experimentally infected with different Zika viruses. Here, we show that Ae. aegypti is mainly responsible for Zika virus transmission having the highest susceptibility to viral infections. Other species play a secondary role in transmission while Culex mosquitoes are largely non-susceptible. Zika strain is expected to significantly modulate transmission efficiency with African strains being more likely to cause an outbreak. As the distribution of Ae. aegypti will doubtless expand with climate change and without new marketed vaccines, all the ingredients are in place to relive a new pandemic of Zika.",
publisher = "Nature Research",
journal = "Nature Communications",
title = "Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project",
volume = "13",
number = "1",
pages = "4490",
doi = "10.1038/s41467-022-32234-y"
}

Obadia, T., Gutierrez-Bugallo, G., Duong, V., Nuñez, A. I., Fernandes, R. S., Kamgang, B., Hery, L., Gomard, Y., Abbo, S. R., Jiolle, D., Glavinić, U., Dupont-Rouzeyrol, M., Atyame, C. M., Pocquet, N., Boyer, S., Dauga, C., Vazeille, M., Yébakima, A., White, M. T., Koenraadt, C. J. M., Mavingui, P., Vega-Rua, A., Veronesi, E., Pijlman, G. P., Paupy, C., Busquets, N., Lourenço-de-Oliveira, R., De Lamballerie, X.,& Failloux, A.. (2022). Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project. in Nature Communications
Nature Research., 13(1), 4490.
https://doi.org/10.1038/s41467-022-32234-y

Obadia T, Gutierrez-Bugallo G, Duong V, Nuñez AI, Fernandes RS, Kamgang B, Hery L, Gomard Y, Abbo SR, Jiolle D, Glavinić U, Dupont-Rouzeyrol M, Atyame CM, Pocquet N, Boyer S, Dauga C, Vazeille M, Yébakima A, White MT, Koenraadt CJM, Mavingui P, Vega-Rua A, Veronesi E, Pijlman GP, Paupy C, Busquets N, Lourenço-de-Oliveira R, De Lamballerie X, Failloux A. Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project. in Nature Communications. 2022;13(1):4490.
doi:10.1038/s41467-022-32234-y .

Obadia, Thomas, Gutierrez-Bugallo, Gladys, Duong, Veasna, Nuñez, Ana I., Fernandes, Rosilainy S., Kamgang, Basile, Hery, Liza, Gomard, Yann, Abbo, Sandra R., Jiolle, Davy, Glavinić, Uroš, Dupont-Rouzeyrol, Myrielle, Atyame, Célestine M., Pocquet, Nicolas, Boyer, Sébastien, Dauga, Catherine, Vazeille, Marie, Yébakima, André, White, Michael T., Koenraadt, Constantianus J. M., Mavingui, Patrick, Vega-Rua, Anubis, Veronesi, Eva, Pijlman, Gorben P., Paupy, Christophe, Busquets, Núria, Lourenço-de-Oliveira, Ricardo, De Lamballerie, Xavier, Failloux, Anna-Bella, "Zika vector competence data reveals risks of outbreaks: the contribution of the European ZIKAlliance project" in Nature Communications, 13, no. 1 (2022):4490,
https://doi.org/10.1038/s41467-022-32234-y . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Varga, Jasmin
AU  - Paslaru, Anca Ioana
AU  - Hauri, Jeannine
AU  - Torgerson, Paul
AU  - Schaffner, Francis
AU  - Veronesi, Eva
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1863
AB  - BACKGROUND: Since the huge epidemic of Zika virus (ZIKV) in Brazil in 2015, questions were raised to understand which mosquito species could transmit the virus. Aedes aegypti has been described as the main vector. However, other Aedes species (e.g. Ae. albopictus and Ae. japonicus) proven to be competent for other flaviviruses (e.g. West Nile, dengue and yellow fever), have been described as potential vectors for ZIKV under laboratory conditions. One of these, the Asian bush mosquito, Ae. japonicus, is widely distributed with high abundances in central-western Europe. In the present study, infection, dissemination and transmission rates of ZIKV (Dak84 strain) in two populations of Ae. japonicus from Switzerland (Zürich) and France (Steinbach, Haut-Rhin) were investigated under constant (27 °C) and fluctuating (14-27 °C, mean 23 °C) temperature regimes. RESULTS: The two populations were each able to transmit ZIKV under both temperature regimes. Infectious virus particles were detected in the saliva of females from both populations, regardless of the incubation temperature regime, from 7 days post-exposure to infectious rabbit blood. The highest amount of plaque forming units (PFU) (400/ml) were recorded 14 days post-oral infection in the Swiss population incubated at a constant temperature. No difference in terms of infection, dissemination and transmission rate were found between mosquito populations. Temperature had no effect on infection rate but the fluctuating temperature regime resulted in higher dissemination rates compared to constant temperature, regardless of the population. Finally, transmission efficiency ranged between 7-23% and 7-10% for the constant temperature and 0-10% and 3-27% under fluctuating temperatures for the Swiss and the French populations, respectively. CONCLUSIONS: To the best of our knowledge, this is the first study confirming vector competence for ZIKV of Ae. japonicus originating from Switzerland and France at realistic summer temperatures under laboratory conditions. Considering the continuous spread of this species in the northern part of Europe and its adaptation at cooler temperatures, preventative control measures should be adopted to prevent possible ZIKV epidemics.
PB  - BMC
T2  - Parasites & Vectors
T2  - Parasites & Vectors
T1  - Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime
VL  - 13
IS  - 1
SP  - 479
DO  - 10.1186/s13071-020-04361-2
ER  - 

@article{
author = "Glavinić, Uroš and Varga, Jasmin and Paslaru, Anca Ioana and Hauri, Jeannine and Torgerson, Paul and Schaffner, Francis and Veronesi, Eva",
year = "2020",
abstract = "BACKGROUND: Since the huge epidemic of Zika virus (ZIKV) in Brazil in 2015, questions were raised to understand which mosquito species could transmit the virus. Aedes aegypti has been described as the main vector. However, other Aedes species (e.g. Ae. albopictus and Ae. japonicus) proven to be competent for other flaviviruses (e.g. West Nile, dengue and yellow fever), have been described as potential vectors for ZIKV under laboratory conditions. One of these, the Asian bush mosquito, Ae. japonicus, is widely distributed with high abundances in central-western Europe. In the present study, infection, dissemination and transmission rates of ZIKV (Dak84 strain) in two populations of Ae. japonicus from Switzerland (Zürich) and France (Steinbach, Haut-Rhin) were investigated under constant (27 °C) and fluctuating (14-27 °C, mean 23 °C) temperature regimes. RESULTS: The two populations were each able to transmit ZIKV under both temperature regimes. Infectious virus particles were detected in the saliva of females from both populations, regardless of the incubation temperature regime, from 7 days post-exposure to infectious rabbit blood. The highest amount of plaque forming units (PFU) (400/ml) were recorded 14 days post-oral infection in the Swiss population incubated at a constant temperature. No difference in terms of infection, dissemination and transmission rate were found between mosquito populations. Temperature had no effect on infection rate but the fluctuating temperature regime resulted in higher dissemination rates compared to constant temperature, regardless of the population. Finally, transmission efficiency ranged between 7-23% and 7-10% for the constant temperature and 0-10% and 3-27% under fluctuating temperatures for the Swiss and the French populations, respectively. CONCLUSIONS: To the best of our knowledge, this is the first study confirming vector competence for ZIKV of Ae. japonicus originating from Switzerland and France at realistic summer temperatures under laboratory conditions. Considering the continuous spread of this species in the northern part of Europe and its adaptation at cooler temperatures, preventative control measures should be adopted to prevent possible ZIKV epidemics.",
publisher = "BMC",
journal = "Parasites & Vectors, Parasites & Vectors",
title = "Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime",
volume = "13",
number = "1",
pages = "479",
doi = "10.1186/s13071-020-04361-2"
}

Glavinić, U., Varga, J., Paslaru, A. I., Hauri, J., Torgerson, P., Schaffner, F.,& Veronesi, E.. (2020). Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime. in Parasites & Vectors
BMC., 13(1), 479.
https://doi.org/10.1186/s13071-020-04361-2

Glavinić U, Varga J, Paslaru AI, Hauri J, Torgerson P, Schaffner F, Veronesi E. Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime. in Parasites & Vectors. 2020;13(1):479.
doi:10.1186/s13071-020-04361-2 .

Glavinić, Uroš, Varga, Jasmin, Paslaru, Anca Ioana, Hauri, Jeannine, Torgerson, Paul, Schaffner, Francis, Veronesi, Eva, "Assessing the role of two populations of Aedes japonicus japonicus for Zika virus transmission under a constant and a fluctuating temperature regime" in Parasites & Vectors, 13, no. 1 (2020):479,
https://doi.org/10.1186/s13071-020-04361-2 . .

TY  - JOUR
AU  - Vasić, Ana
AU  - Zdravković, Nemanja
AU  - Anita, Dragos
AU  - Bojkovski, Jovan
AU  - Marinov, Mihai
AU  - Mathis, Alexander
AU  - Niculaua, Marius
AU  - Oslobanu, Elena Luanda
AU  - Pavlović, Ivan
AU  - Petrić, Dusan
AU  - Pflueger, Valentin
AU  - Pudar, Dubravka
AU  - Savuta, Gheorghe
AU  - Simeunović, Predrag
AU  - Veronesi, Eva
AU  - Silaghi, Cornelia
AU  - Anita, Adriana
AU  - Anton, Ioana Alexandra
AU  - Cimpan, Andrei
AU  - Ciuca, Lavinia
AU  - Crivei, Luciana
AU  - Cojkić, Aleksandar
AU  - Davitkov, Darko
AU  - Drašković, Vladimir
AU  - Gajić, Bojan
AU  - Glavinić, Uroš
AU  - Ivanescu, Maria-Larisa
AU  - Kavran, Mihaela
AU  - Lupu, Andrei-Cristian
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Prodanović, Radiša
AU  - Radanović, Oliver
AU  - Raileanu, Cristian
AU  - Raileanu, Stefan
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Vaselek, Slavica
AU  - Đurić, Miloje
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1740
AB  - BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia
VL  - 12
SP  - 61
DO  - 10.1186/s13071-019-3292-3
ER  - 

@article{
author = "Vasić, Ana and Zdravković, Nemanja and Anita, Dragos and Bojkovski, Jovan and Marinov, Mihai and Mathis, Alexander and Niculaua, Marius and Oslobanu, Elena Luanda and Pavlović, Ivan and Petrić, Dusan and Pflueger, Valentin and Pudar, Dubravka and Savuta, Gheorghe and Simeunović, Predrag and Veronesi, Eva and Silaghi, Cornelia and Anita, Adriana and Anton, Ioana Alexandra and Cimpan, Andrei and Ciuca, Lavinia and Crivei, Luciana and Cojkić, Aleksandar and Davitkov, Darko and Drašković, Vladimir and Gajić, Bojan and Glavinić, Uroš and Ivanescu, Maria-Larisa and Kavran, Mihaela and Lupu, Andrei-Cristian and Mindru, Raluca and Porea, Daniela and Prodanović, Radiša and Radanović, Oliver and Raileanu, Cristian and Raileanu, Stefan and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Vaselek, Slavica and Đurić, Miloje",
year = "2019",
abstract = "BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia",
volume = "12",
pages = "61",
doi = "10.1186/s13071-019-3292-3"
}

Vasić, A., Zdravković, N., Anita, D., Bojkovski, J., Marinov, M., Mathis, A., Niculaua, M., Oslobanu, E. L., Pavlović, I., Petrić, D., Pflueger, V., Pudar, D., Savuta, G., Simeunović, P., Veronesi, E., Silaghi, C., Anita, A., Anton, I. A., Cimpan, A., Ciuca, L., Crivei, L., Cojkić, A., Davitkov, D., Drašković, V., Gajić, B., Glavinić, U., Ivanescu, M., Kavran, M., Lupu, A., Mindru, R., Porea, D., Prodanović, R., Radanović, O., Raileanu, C., Raileanu, S., Ristanić, M., Roman, C., Stanišić, L., Vaselek, S.,& Đurić, M.. (2019). Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors
BMC, London., 12, 61.
https://doi.org/10.1186/s13071-019-3292-3

Vasić A, Zdravković N, Anita D, Bojkovski J, Marinov M, Mathis A, Niculaua M, Oslobanu EL, Pavlović I, Petrić D, Pflueger V, Pudar D, Savuta G, Simeunović P, Veronesi E, Silaghi C, Anita A, Anton IA, Cimpan A, Ciuca L, Crivei L, Cojkić A, Davitkov D, Drašković V, Gajić B, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Prodanović R, Radanović O, Raileanu C, Raileanu S, Ristanić M, Roman C, Stanišić L, Vaselek S, Đurić M. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors. 2019;12:61.
doi:10.1186/s13071-019-3292-3 .

Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia" in Parasites & Vectors, 12 (2019):61,
https://doi.org/10.1186/s13071-019-3292-3 . .