TY  - JOUR
AU  - Podpecan, Ozbalt
AU  - Zrimsek, Petra
AU  - Mrkun, Janko
AU  - Golicnik, Marko
AU  - Radovanović, Anita
AU  - Jovanović, Ljubomir
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1823
AB  - The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Italian Journal of Animal Science
T1  - Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows
VL  - 19
IS  - 1
SP  - 303
EP  - 309
DO  - 10.1080/1828051X.2020.1740064
ER  - 

@article{
author = "Podpecan, Ozbalt and Zrimsek, Petra and Mrkun, Janko and Golicnik, Marko and Radovanović, Anita and Jovanović, Ljubomir and Vujanac, Ivan and Prodanović, Radiša and Kirovski, Danijela",
year = "2020",
abstract = "The objective was to determine optimal thresholds of blood variables that are associated with liver lipid and glycogen content in dairy cows. Liver specimens of 15 Holstein dairy cows were taken 2 weeks postpartum (PP) and used for determination of lipid and glycogen content, respectively. Blood samples were collected weekly, starting from week 2 antepartum (AP) up to week 2 PP. Selected blood parameters and body condition scoring (BCS) were evaluated through receiver operating characteristic curve analysis as indication of fat and glycogen content in the liver. Postpartal low liver glycogen content (mode < 2.0, semiquantitative analysis of glycogen content in the liver tissue samples) can be indicated if values are higher than 0.16 mmol/L, and 3.5 mmol/L for NEFA, and glucose, respectively, and lower than 0.65 mmol/L and 17.94 mu U/mL for BHBA and insulin, respectively, 2 weeks AP. Postaprtal high liver fat content (>20%, stereological quantification of fat content in the liver tissue samples) can be indicated if values are higher than 3.5 for BCS at week 2 AP. Optimal time for indicating both low liver glycogen/high fat content is 1 week PP, with LDL < cut off point set at 0.18 mmol/L and BHBA > cut off point set at 0.88 mmol/L. Established cut off values in this study can be used for indication of fat and glycogen content in the liver of dairy cows.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Italian Journal of Animal Science",
title = "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows",
volume = "19",
number = "1",
pages = "303-309",
doi = "10.1080/1828051X.2020.1740064"
}

Podpecan, O., Zrimsek, P., Mrkun, J., Golicnik, M., Radovanović, A., Jovanović, L., Vujanac, I., Prodanović, R.,& Kirovski, D.. (2020). Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science
Taylor & Francis Ltd, Abingdon., 19(1), 303-309.
https://doi.org/10.1080/1828051X.2020.1740064

Podpecan O, Zrimsek P, Mrkun J, Golicnik M, Radovanović A, Jovanović L, Vujanac I, Prodanović R, Kirovski D. Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows. in Italian Journal of Animal Science. 2020;19(1):303-309.
doi:10.1080/1828051X.2020.1740064 .

Podpecan, Ozbalt, Zrimsek, Petra, Mrkun, Janko, Golicnik, Marko, Radovanović, Anita, Jovanović, Ljubomir, Vujanac, Ivan, Prodanović, Radiša, Kirovski, Danijela, "Tresholds of blood variables obtained by receiver operating characteristic analysis for indication of fat and glycogen content in the liver of postpartum dairy cows" in Italian Journal of Animal Science, 19, no. 1 (2020):303-309,
https://doi.org/10.1080/1828051X.2020.1740064 . .

TY  - JOUR
AU  - Nedić, Sreten
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Jovanović, Ljubomir
AU  - Kobal, Silvestra
AU  - Snoj, Tomaz
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1592
AB  - The aim of this study was to compare hair corticosteroid concentrations in ectoparasiticide-treated (n = 10) and non-treated (n = 12) Holstein cows. Animals in the treated group received cyfluthrin three times, on days 0, 28 and 56 of the experiment. Hair samples were collected from all cows on days 0, 21, 42, 63, and 84 of the experiment for the determination of cortisol and corticosterone concentrations using immunoassays. The respective hair cortisol concentrations in the treated group averaged 209.78, 165.10, 260.78, 177.44 and 183.11 ng/g, while in the non-treated group hair cortisol concentrations averaged 172.58, 243.58, 309.73, 199.75 and 207.09 ng/g. These results indicate that the control of ectoparasites reduced hair cortisol levels in dairy cows. The respective hair corticosterone concentrations in the treated group averaged 19.06, 22.95, 21.95, 21.60 and 24.84 ng/g and in the non-treated group the hair corticosterone concentrations averaged 17.28, 21.94, 34.05, 26.27 and 26.91 ng/g. The results suggest that long-term stress can be estimated better by the determination of hair cortisol rather than corticosterone concentrations.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows - short communication
VL  - 66
IS  - 3
SP  - 390
EP  - 393
DO  - 10.1556/004.2018.035
ER  - 

@article{
author = "Nedić, Sreten and Kirovski, Danijela and Vujanac, Ivan and Prodanović, Radiša and Jovanović, Ljubomir and Kobal, Silvestra and Snoj, Tomaz",
year = "2018",
abstract = "The aim of this study was to compare hair corticosteroid concentrations in ectoparasiticide-treated (n = 10) and non-treated (n = 12) Holstein cows. Animals in the treated group received cyfluthrin three times, on days 0, 28 and 56 of the experiment. Hair samples were collected from all cows on days 0, 21, 42, 63, and 84 of the experiment for the determination of cortisol and corticosterone concentrations using immunoassays. The respective hair cortisol concentrations in the treated group averaged 209.78, 165.10, 260.78, 177.44 and 183.11 ng/g, while in the non-treated group hair cortisol concentrations averaged 172.58, 243.58, 309.73, 199.75 and 207.09 ng/g. These results indicate that the control of ectoparasites reduced hair cortisol levels in dairy cows. The respective hair corticosterone concentrations in the treated group averaged 19.06, 22.95, 21.95, 21.60 and 24.84 ng/g and in the non-treated group the hair corticosterone concentrations averaged 17.28, 21.94, 34.05, 26.27 and 26.91 ng/g. The results suggest that long-term stress can be estimated better by the determination of hair cortisol rather than corticosterone concentrations.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows - short communication",
volume = "66",
number = "3",
pages = "390-393",
doi = "10.1556/004.2018.035"
}

Nedić, S., Kirovski, D., Vujanac, I., Prodanović, R., Jovanović, L., Kobal, S.,& Snoj, T.. (2018). Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows - short communication. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 66(3), 390-393.
https://doi.org/10.1556/004.2018.035

Nedić S, Kirovski D, Vujanac I, Prodanović R, Jovanović L, Kobal S, Snoj T. Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows - short communication. in Acta Veterinaria Hungarica. 2018;66(3):390-393.
doi:10.1556/004.2018.035 .

Nedić, Sreten, Kirovski, Danijela, Vujanac, Ivan, Prodanović, Radiša, Jovanović, Ljubomir, Kobal, Silvestra, Snoj, Tomaz, "Preventing ectoparasite infestations reduces glucocorticoid concentrations in the hair of cows - short communication" in Acta Veterinaria Hungarica, 66, no. 3 (2018):390-393,
https://doi.org/10.1556/004.2018.035 . .

TY  - JOUR
AU  - Vasiljević, Maja
AU  - Krstić, Vanja
AU  - Stanković, Sanja
AU  - Zrimsek, Petra
AU  - Svete, Alenka Nemec
AU  - Seliskar, Alenka
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1582
AB  - Objective To investigate changes in serum cardiac troponin I (cTnI) concentrations in dogs in which medetomidine was used for sedation or for premedication prior to anaesthesia with propofol and sevoflurane. Study design Prospective clinical study. Animals A total of 66 client-owned dogs. Methods The dogs were sedated with medetomidine (0.04 mg kg(-1)) intravenously (IV) (group M; n = 20) and left to breath room air or anaesthetized with propofol (6.5 +/- 0.76 mg kg(-1) IV) and sevoflurane (4.5% vaporizer setting) in oxygen (group P + S; n = 20) or with medetomidine (0.04 mg kg(-1) IV), propofol (1.92 +/- 0.63 mg kg(-1)) and sevoflurane (3% vaporizer setting) in oxygen (group M + P + S; n = 26), respectively. After 35 minutes, medetomidine was antagonized with atipamezole (0.1 mg kg(-1) intramuscularly). Blood samples for serum cTnI determination were taken before sedation or anaesthesia, 6 and 12 hours and 4 days thereafter. Serum cTnI concentrations were measured with the Architect STAT Troponin-I assay. Results Before sedation or anaesthesia, cTnI concentrations were above the detection limit in 22 out of 66 (33%) of dogs. Compared to basal values, cTnI concentrations significantly increased at 6 and 12 hours in all groups and at day 4 in group M. There were no differences in cTnI concentration between groups at baseline, at 6 hours and at 4 days. At 12 hours, cTnI concentrations were significantly higher in groups M and P + S, respectively, compared to group M + P + S. Conclusions and clinical relevance Oxygenation during anaesthesia and reduction of propofol and sevoflurane dose due to the sparing effects of medetomidine might have played a role in alleviation of myocardial hypoxic injury as indicated by the less severe and short-lived increase of cTnI in the M + P + S group.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Anaesthesia and Analgesia
T1  - Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction
VL  - 45
IS  - 6
SP  - 745
EP  - 753
DO  - 10.1016/j.vaa.2018.07.003
ER  - 

@article{
author = "Vasiljević, Maja and Krstić, Vanja and Stanković, Sanja and Zrimsek, Petra and Svete, Alenka Nemec and Seliskar, Alenka",
year = "2018",
abstract = "Objective To investigate changes in serum cardiac troponin I (cTnI) concentrations in dogs in which medetomidine was used for sedation or for premedication prior to anaesthesia with propofol and sevoflurane. Study design Prospective clinical study. Animals A total of 66 client-owned dogs. Methods The dogs were sedated with medetomidine (0.04 mg kg(-1)) intravenously (IV) (group M; n = 20) and left to breath room air or anaesthetized with propofol (6.5 +/- 0.76 mg kg(-1) IV) and sevoflurane (4.5% vaporizer setting) in oxygen (group P + S; n = 20) or with medetomidine (0.04 mg kg(-1) IV), propofol (1.92 +/- 0.63 mg kg(-1)) and sevoflurane (3% vaporizer setting) in oxygen (group M + P + S; n = 26), respectively. After 35 minutes, medetomidine was antagonized with atipamezole (0.1 mg kg(-1) intramuscularly). Blood samples for serum cTnI determination were taken before sedation or anaesthesia, 6 and 12 hours and 4 days thereafter. Serum cTnI concentrations were measured with the Architect STAT Troponin-I assay. Results Before sedation or anaesthesia, cTnI concentrations were above the detection limit in 22 out of 66 (33%) of dogs. Compared to basal values, cTnI concentrations significantly increased at 6 and 12 hours in all groups and at day 4 in group M. There were no differences in cTnI concentration between groups at baseline, at 6 hours and at 4 days. At 12 hours, cTnI concentrations were significantly higher in groups M and P + S, respectively, compared to group M + P + S. Conclusions and clinical relevance Oxygenation during anaesthesia and reduction of propofol and sevoflurane dose due to the sparing effects of medetomidine might have played a role in alleviation of myocardial hypoxic injury as indicated by the less severe and short-lived increase of cTnI in the M + P + S group.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Anaesthesia and Analgesia",
title = "Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction",
volume = "45",
number = "6",
pages = "745-753",
doi = "10.1016/j.vaa.2018.07.003"
}

Vasiljević, M., Krstić, V., Stanković, S., Zrimsek, P., Svete, A. N.,& Seliskar, A.. (2018). Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction. in Veterinary Anaesthesia and Analgesia
Elsevier Science Bv, Amsterdam., 45(6), 745-753.
https://doi.org/10.1016/j.vaa.2018.07.003

Vasiljević M, Krstić V, Stanković S, Zrimsek P, Svete AN, Seliskar A. Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction. in Veterinary Anaesthesia and Analgesia. 2018;45(6):745-753.
doi:10.1016/j.vaa.2018.07.003 .

Vasiljević, Maja, Krstić, Vanja, Stanković, Sanja, Zrimsek, Petra, Svete, Alenka Nemec, Seliskar, Alenka, "Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction" in Veterinary Anaesthesia and Analgesia, 45, no. 6 (2018):745-753,
https://doi.org/10.1016/j.vaa.2018.07.003 . .

TY  - JOUR
AU  - Vasiljević, Maja
AU  - Krstić, Vanja
AU  - Stanković, Sanja
AU  - Zrimsek, Petra
AU  - Svete, Alenka Nemec
AU  - Seliskar, Alenka
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1851
AB  - Objective To investigate changes in serum cardiac troponin I (cTnI) concentrations in dogs in which medetomidine was used for sedation or for premedication prior to anaesthesia with propofol and sevoflurane. Study design Prospective clinical study. Animals A total of 66 client-owned dogs. Methods The dogs were sedated with medetomidine (0.04 mg kg(-1)) intravenously (IV) (group M; n = 20) and left to breath room air or anaesthetized with propofol (6.5 +/- 0.76 mg kg(-1) IV) and sevoflurane (4.5% vaporizer setting) in oxygen (group P + S; n = 20) or with medetomidine (0.04 mg kg(-1) IV), propofol (1.92 +/- 0.63 mg kg(-1)) and sevoflurane (3% vaporizer setting) in oxygen (group M + P + S; n = 26), respectively. After 35 minutes, medetomidine was antagonized with atipamezole (0.1 mg kg(-1) intramuscularly). Blood samples for serum cTnI determination were taken before sedation or anaesthesia, 6 and 12 hours and 4 days thereafter. Serum cTnI concentrations were measured with the Architect STAT Troponin-I assay. Results Before sedation or anaesthesia, cTnI concentrations were above the detection limit in 22 out of 66 (33%) of dogs. Compared to basal values, cTnI concentrations significantly increased at 6 and 12 hours in all groups and at day 4 in group M. There were no differences in cTnI concentration between groups at baseline, at 6 hours and at 4 days. At 12 hours, cTnI concentrations were significantly higher in groups M and P + S, respectively, compared to group M + P + S. Conclusions and clinical relevance Oxygenation during anaesthesia and reduction of propofol and sevoflurane dose due to the sparing effects of medetomidine might have played a role in alleviation of myocardial hypoxic injury as indicated by the less severe and short-lived increase of cTnI in the M + P + S group.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Anaesthesia and Analgesia
T1  - Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction
VL  - 45
IS  - 6
SP  - 745
EP  - 753
DO  - 10.1016/j.vaa.2018.07.003
ER  - 

@article{
author = "Vasiljević, Maja and Krstić, Vanja and Stanković, Sanja and Zrimsek, Petra and Svete, Alenka Nemec and Seliskar, Alenka",
year = "2018",
abstract = "Objective To investigate changes in serum cardiac troponin I (cTnI) concentrations in dogs in which medetomidine was used for sedation or for premedication prior to anaesthesia with propofol and sevoflurane. Study design Prospective clinical study. Animals A total of 66 client-owned dogs. Methods The dogs were sedated with medetomidine (0.04 mg kg(-1)) intravenously (IV) (group M; n = 20) and left to breath room air or anaesthetized with propofol (6.5 +/- 0.76 mg kg(-1) IV) and sevoflurane (4.5% vaporizer setting) in oxygen (group P + S; n = 20) or with medetomidine (0.04 mg kg(-1) IV), propofol (1.92 +/- 0.63 mg kg(-1)) and sevoflurane (3% vaporizer setting) in oxygen (group M + P + S; n = 26), respectively. After 35 minutes, medetomidine was antagonized with atipamezole (0.1 mg kg(-1) intramuscularly). Blood samples for serum cTnI determination were taken before sedation or anaesthesia, 6 and 12 hours and 4 days thereafter. Serum cTnI concentrations were measured with the Architect STAT Troponin-I assay. Results Before sedation or anaesthesia, cTnI concentrations were above the detection limit in 22 out of 66 (33%) of dogs. Compared to basal values, cTnI concentrations significantly increased at 6 and 12 hours in all groups and at day 4 in group M. There were no differences in cTnI concentration between groups at baseline, at 6 hours and at 4 days. At 12 hours, cTnI concentrations were significantly higher in groups M and P + S, respectively, compared to group M + P + S. Conclusions and clinical relevance Oxygenation during anaesthesia and reduction of propofol and sevoflurane dose due to the sparing effects of medetomidine might have played a role in alleviation of myocardial hypoxic injury as indicated by the less severe and short-lived increase of cTnI in the M + P + S group.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Anaesthesia and Analgesia",
title = "Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction",
volume = "45",
number = "6",
pages = "745-753",
doi = "10.1016/j.vaa.2018.07.003"
}

Vasiljević, M., Krstić, V., Stanković, S., Zrimsek, P., Svete, A. N.,& Seliskar, A.. (2018). Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction. in Veterinary Anaesthesia and Analgesia
Elsevier Science Bv, Amsterdam., 45(6), 745-753.
https://doi.org/10.1016/j.vaa.2018.07.003

Vasiljević M, Krstić V, Stanković S, Zrimsek P, Svete AN, Seliskar A. Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction. in Veterinary Anaesthesia and Analgesia. 2018;45(6):745-753.
doi:10.1016/j.vaa.2018.07.003 .

Vasiljević, Maja, Krstić, Vanja, Stanković, Sanja, Zrimsek, Petra, Svete, Alenka Nemec, Seliskar, Alenka, "Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction" in Veterinary Anaesthesia and Analgesia, 45, no. 6 (2018):745-753,
https://doi.org/10.1016/j.vaa.2018.07.003 . .

TY  - JOUR
AU  - Nedić, Sreten
AU  - Pantelić, Marija
AU  - Vranješ-Đurić, Sanja
AU  - Nedić, Drago
AU  - Jovanović, Ljubomir
AU  - Cebulj-Kadunc, Nina
AU  - Kobal, Silvestra
AU  - Snoj, Tomaz
AU  - Kirovski, Danijela
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1514
AB  - Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P<0.05 and P<0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P<0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P<0.01 and P<0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P<0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.
PB  - Univ Ljubljana, Veterinary Faculty, Ljubljana
T2  - Slovenian Veterinary Research
T1  - Cortisol concentrations in hair, blood and milk of holstein and busha cattle
VL  - 54
IS  - 4
SP  - 163
EP  - 172
DO  - 10.26873/SVR-398-2017
ER  - 

@article{
author = "Nedić, Sreten and Pantelić, Marija and Vranješ-Đurić, Sanja and Nedić, Drago and Jovanović, Ljubomir and Cebulj-Kadunc, Nina and Kobal, Silvestra and Snoj, Tomaz and Kirovski, Danijela",
year = "2017",
abstract = "Cortisol levels were measured in hair, blood and milk in two different cattle breeds, kept under different breeding conditions and with different genetic merit for milk production. Cows and heifers of Holstein and Busha breeds were selected for the study. Cortisol concentration was determined by immunoassays. Cortisol accumulation was determined in proximal (close to the skin) and distal (far from the skin) segments of the hair shaft. The influence of hair colour and washing prior to extraction and analysis was also examined in order to establish additional factors that may have an impact on hair cortisol concentrations. Concentrations of cortisol determined in the proximal and distal segments of the shaft were significantly higher in Holstein than Busha cows and heifers (P<0.05 and P<0.01, respectively). In Holstein cows, no significant difference was found between concentrations in black and white hair. In hair washed with isopropanol, cortisol concentration was significantly lower compared to unwashed hair (P<0.01). Thus, cortisol concentration in hair varies with the technique of hair processing (washing), but not with colour in Holstein cows. Blood serum cortisol concentrations in Holstein cows and heifers were significantly higher than in Busha cows and heifers, (P<0.01 and P<0.05, respectively). Milk cortisol in Holstein cows was significantly higher than in Busha cows (P<0.05). The higher cortisol concentrations in Holstein cows are assumed to be the result of intensive breeding and physiological adaptation to high milk production.",
publisher = "Univ Ljubljana, Veterinary Faculty, Ljubljana",
journal = "Slovenian Veterinary Research",
title = "Cortisol concentrations in hair, blood and milk of holstein and busha cattle",
volume = "54",
number = "4",
pages = "163-172",
doi = "10.26873/SVR-398-2017"
}

Nedić, S., Pantelić, M., Vranješ-Đurić, S., Nedić, D., Jovanović, L., Cebulj-Kadunc, N., Kobal, S., Snoj, T.,& Kirovski, D.. (2017). Cortisol concentrations in hair, blood and milk of holstein and busha cattle. in Slovenian Veterinary Research
Univ Ljubljana, Veterinary Faculty, Ljubljana., 54(4), 163-172.
https://doi.org/10.26873/SVR-398-2017

Nedić S, Pantelić M, Vranješ-Đurić S, Nedić D, Jovanović L, Cebulj-Kadunc N, Kobal S, Snoj T, Kirovski D. Cortisol concentrations in hair, blood and milk of holstein and busha cattle. in Slovenian Veterinary Research. 2017;54(4):163-172.
doi:10.26873/SVR-398-2017 .

Nedić, Sreten, Pantelić, Marija, Vranješ-Đurić, Sanja, Nedić, Drago, Jovanović, Ljubomir, Cebulj-Kadunc, Nina, Kobal, Silvestra, Snoj, Tomaz, Kirovski, Danijela, "Cortisol concentrations in hair, blood and milk of holstein and busha cattle" in Slovenian Veterinary Research, 54, no. 4 (2017):163-172,
https://doi.org/10.26873/SVR-398-2017 . .