TY  - JOUR
AU  - Ivanović, Zoran
AU  - Kovačević-Filipović, Milica
AU  - Jeanne, Michel
AU  - Ardilouze, Leslie
AU  - Bertot, Anne
AU  - Szyporta, Milene
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Vlaški, Marija
AU  - Milpied, Noel
AU  - Pavlović, Mirjana
AU  - Praloran, Vincent
AU  - Boiron, Jean-Michel
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/753
AB  - BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""
VL  - 50
IS  - 1
SP  - 120
EP  - 127
DO  - 10.1111/j.1537-2995.2009.02436.x
ER  - 

@article{
author = "Ivanović, Zoran and Kovačević-Filipović, Milica and Jeanne, Michel and Ardilouze, Leslie and Bertot, Anne and Szyporta, Milene and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Vlaški, Marija and Milpied, Noel and Pavlović, Mirjana and Praloran, Vincent and Boiron, Jean-Michel",
year = "2010",
abstract = "BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""",
volume = "50",
number = "1",
pages = "120-127",
doi = "10.1111/j.1537-2995.2009.02436.x"
}

Ivanović, Z., Kovačević-Filipović, M., Jeanne, M., Ardilouze, L., Bertot, A., Szyporta, M., Hermitte, F., Lafarge, X., Duchez, P., Vlaški, M., Milpied, N., Pavlović, M., Praloran, V.,& Boiron, J.. (2010). CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion
Wiley, Hoboken., 50(1), 120-127.
https://doi.org/10.1111/j.1537-2995.2009.02436.x

Ivanović Z, Kovačević-Filipović M, Jeanne M, Ardilouze L, Bertot A, Szyporta M, Hermitte F, Lafarge X, Duchez P, Vlaški M, Milpied N, Pavlović M, Praloran V, Boiron J. CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion. 2010;50(1):120-127.
doi:10.1111/j.1537-2995.2009.02436.x .

Ivanović, Zoran, Kovačević-Filipović, Milica, Jeanne, Michel, Ardilouze, Leslie, Bertot, Anne, Szyporta, Milene, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Vlaški, Marija, Milpied, Noel, Pavlović, Mirjana, Praloran, Vincent, Boiron, Jean-Michel, "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""" in Transfusion, 50, no. 1 (2010):120-127,
https://doi.org/10.1111/j.1537-2995.2009.02436.x . .