TY  - JOUR
AU  - Jeanne, Michel
AU  - Kovačević-Filipović, Milica
AU  - Szyporta, Milene
AU  - Vlaški, Marija
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Boiron, Jean-Michel
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/585
AB  - BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia
VL  - 49
IS  - 8
SP  - 1738
EP  - 1746
DO  - 10.1111/j.1537-2995.2009.02191.x
ER  - 

@article{
author = "Jeanne, Michel and Kovačević-Filipović, Milica and Szyporta, Milene and Vlaški, Marija and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Boiron, Jean-Michel and Praloran, Vincent and Ivanović, Zoran",
year = "2009",
abstract = "BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia",
volume = "49",
number = "8",
pages = "1738-1746",
doi = "10.1111/j.1537-2995.2009.02191.x"
}

Jeanne, M., Kovačević-Filipović, M., Szyporta, M., Vlaški, M., Hermitte, F., Lafarge, X., Duchez, P., Boiron, J., Praloran, V.,& Ivanović, Z.. (2009). Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion
Wiley, Hoboken., 49(8), 1738-1746.
https://doi.org/10.1111/j.1537-2995.2009.02191.x

Jeanne M, Kovačević-Filipović M, Szyporta M, Vlaški M, Hermitte F, Lafarge X, Duchez P, Boiron J, Praloran V, Ivanović Z. Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion. 2009;49(8):1738-1746.
doi:10.1111/j.1537-2995.2009.02191.x .

Jeanne, Michel, Kovačević-Filipović, Milica, Szyporta, Milene, Vlaški, Marija, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Boiron, Jean-Michel, Praloran, Vincent, Ivanović, Zoran, "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia" in Transfusion, 49, no. 8 (2009):1738-1746,
https://doi.org/10.1111/j.1537-2995.2009.02191.x . .