TY  - JOUR
AU  - Ivanović, Zoran
AU  - Kovačević-Filipović, Milica
AU  - Jeanne, Michel
AU  - Ardilouze, Leslie
AU  - Bertot, Anne
AU  - Szyporta, Milene
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Vlaški, Marija
AU  - Milpied, Noel
AU  - Pavlović, Mirjana
AU  - Praloran, Vincent
AU  - Boiron, Jean-Michel
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/753
AB  - BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""
VL  - 50
IS  - 1
SP  - 120
EP  - 127
DO  - 10.1111/j.1537-2995.2009.02436.x
ER  - 

@article{
author = "Ivanović, Zoran and Kovačević-Filipović, Milica and Jeanne, Michel and Ardilouze, Leslie and Bertot, Anne and Szyporta, Milene and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Vlaški, Marija and Milpied, Noel and Pavlović, Mirjana and Praloran, Vincent and Boiron, Jean-Michel",
year = "2010",
abstract = "BACKGROUND: The classification of patients into good or poor mobilizers is based on CD34+ cell count in their peripheral blood (PB) after granulocyte-colony-stimulating factor (G-CSF) injection. We hypothesized that, apart from their mobilization from marrow to the blood, the response to G-CSF of CD34+ cells also includes activation of proliferation, metabolic activity, and proliferative capacity. STUDY DESIGN AND METHODS: Mobilized PB CD34+ cells purified from samples obtained by cytapheresis of multiple myeloma or non-Hodgkins lymphoma patients of both good (> 50 CD34+ cells/mu L) and poor (< 50 CD34+ cells/mu L) mobilizers were studied. The initial cell cycle state of CD34+ cells after selection and their kinetics of activation (exit from G(0) phase) during ex vivo culture were analyzed. Their proliferative capacity was estimated on the basis of ex vivo generation of total cells, CD34+ cells, and colony-forming cells (CFCs), in a standardized expansion culture. Indirect insight in metabolic activity was obtained on the basis of their survival (viability and apoptosis follow-up) during the 7-day-long conservation in hypothermia (4 degrees C) in the air or in atmosphere containing 3% O-2/6% CO2. RESULTS: CD34+ cells obtained from good mobilizers were in lower proportion in the G(0) phase, their activation in a cytokine-stimulated culture was accelerated, and they exhibited a lower ex vivo expansion efficiency than those from poor mobilizers. The resistance to hypothermia of good immobilizers CD34+ cells is impaired. CONCLUSION: A good response to G-CSF mobilization treatment is associated with a higher degree of proliferative and metabolic activation of mobilized CD34+ cells with a decrease in their expansion capacity.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""",
volume = "50",
number = "1",
pages = "120-127",
doi = "10.1111/j.1537-2995.2009.02436.x"
}

Ivanović, Z., Kovačević-Filipović, M., Jeanne, M., Ardilouze, L., Bertot, A., Szyporta, M., Hermitte, F., Lafarge, X., Duchez, P., Vlaški, M., Milpied, N., Pavlović, M., Praloran, V.,& Boiron, J.. (2010). CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion
Wiley, Hoboken., 50(1), 120-127.
https://doi.org/10.1111/j.1537-2995.2009.02436.x

Ivanović Z, Kovačević-Filipović M, Jeanne M, Ardilouze L, Bertot A, Szyporta M, Hermitte F, Lafarge X, Duchez P, Vlaški M, Milpied N, Pavlović M, Praloran V, Boiron J. CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers"". in Transfusion. 2010;50(1):120-127.
doi:10.1111/j.1537-2995.2009.02436.x .

Ivanović, Zoran, Kovačević-Filipović, Milica, Jeanne, Michel, Ardilouze, Leslie, Bertot, Anne, Szyporta, Milene, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Vlaški, Marija, Milpied, Noel, Pavlović, Mirjana, Praloran, Vincent, Boiron, Jean-Michel, "CD34+cells obtained from ""good mobilizers"" are more activated and exhibit lower ex vivo expansion efficiency than their counterparts from ""poor mobilizers""" in Transfusion, 50, no. 1 (2010):120-127,
https://doi.org/10.1111/j.1537-2995.2009.02436.x . .

TY  - JOUR
AU  - Jeanne, Michel
AU  - Kovačević-Filipović, Milica
AU  - Szyporta, Milene
AU  - Vlaški, Marija
AU  - Hermitte, Francis
AU  - Lafarge, Xavier
AU  - Duchez, Pascale
AU  - Boiron, Jean-Michel
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/585
AB  - BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.
PB  - Wiley, Hoboken
T2  - Transfusion
T1  - Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia
VL  - 49
IS  - 8
SP  - 1738
EP  - 1746
DO  - 10.1111/j.1537-2995.2009.02191.x
ER  - 

@article{
author = "Jeanne, Michel and Kovačević-Filipović, Milica and Szyporta, Milene and Vlaški, Marija and Hermitte, Francis and Lafarge, Xavier and Duchez, Pascale and Boiron, Jean-Michel and Praloran, Vincent and Ivanović, Zoran",
year = "2009",
abstract = "BACKGROUND: During short-term storage of hematopoietic cells (HCs) at 4 degrees C a substantial decline in number and in functional capacity of progenitors occurs after 3 days. We hypothesized that physiologic O-2 and CO2 concentrations of hematopoietic tissue microenvironment (approx. 3% O-2 and approx. 6% CO2) could improve cell viability and functionality during storage at 4 degrees C. STUDY DESIGN AND METHODS: Mobilized peripheral blood (PB) CD34+ cells from multiple myeloma or non-Hodgkins lymphoma patients were stored in flasks containing air (approx. 20% O-2 and approx. 0.05% CO2) or 3% O-2/6% CO2 atmosphere, for 3, 5, and 7 days at 4 degrees C. The total number of cells, the number of cells in G0 or G1 phase of cell cycle, and the apoptosis rate were determined. The functional capacity of stored cells was assessed by the capacity of progenitors to form colonies in methylcellulose (colony-forming cells [CFCs]) and of stem cells to repopulate the bone marrow (BM) of immunodeficient mice (SCID-repopulating cell [SRC] assay). RESULTS: The total number of viable cells and cells in G1 phase as well as the number of total CFCs were significantly higher at 3% O-2/6% CO2 than in air at all time points. Cells in G0 phase and SRC were equally preserved in both conditions. CONCLUSION: Atmosphere with low O-2 and high CO2 concentration (3% O-2/6% CO2) in hypothermia (+4 degrees C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.",
publisher = "Wiley, Hoboken",
journal = "Transfusion",
title = "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia",
volume = "49",
number = "8",
pages = "1738-1746",
doi = "10.1111/j.1537-2995.2009.02191.x"
}

Jeanne, M., Kovačević-Filipović, M., Szyporta, M., Vlaški, M., Hermitte, F., Lafarge, X., Duchez, P., Boiron, J., Praloran, V.,& Ivanović, Z.. (2009). Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion
Wiley, Hoboken., 49(8), 1738-1746.
https://doi.org/10.1111/j.1537-2995.2009.02191.x

Jeanne M, Kovačević-Filipović M, Szyporta M, Vlaški M, Hermitte F, Lafarge X, Duchez P, Boiron J, Praloran V, Ivanović Z. Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia. in Transfusion. 2009;49(8):1738-1746.
doi:10.1111/j.1537-2995.2009.02191.x .

Jeanne, Michel, Kovačević-Filipović, Milica, Szyporta, Milene, Vlaški, Marija, Hermitte, Francis, Lafarge, Xavier, Duchez, Pascale, Boiron, Jean-Michel, Praloran, Vincent, Ivanović, Zoran, "Low-oxygen and high-carbon-dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia" in Transfusion, 49, no. 8 (2009):1738-1746,
https://doi.org/10.1111/j.1537-2995.2009.02191.x . .