@article{
author = "Vlaški, Marija and Negroni, Luc and Kovačević-Filipović, Milica and Guibert, Christelle and de la Grange, Philippe Brunet and Rossignol, Rodrigue and Chevaleyre, Jean and Duchez, Pascale and Lafarge, Xavier and Praloran, Vincent and Schmitter, Jean-Marie and Ivanović, Zoran",
year = "2014",
abstract = "We analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2/9% CO2) on the maintenance of functional cord blood CD34+ hematopoietic stem and progenitor cells in severe hypothermia (4 degrees C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) level of hematopoietic stem cells as detected in vivo on the basis of hematopoietic repopulation of immunodeficient miceshort-term scid repopulating cells (SRC). Conversely, in the atmospheric air (20% O2/0.05% CO2), usual condition used for cell storage at 4 degrees C, stem cell activity was significantly decreased. Also, HH doubled the survival of CD34+ cells and committed progenitors (CFCs) with respect to the atmospheric air (60% vs. 30%, respectively). Improved cell maintenance in HH was associated with higher proportion of aldehyde dehydrogenase (ALDH) positive cells. Cell-protective effects are associated with an improved maintenance of the plasma and mitochondrial membrane potential and with a conversion to the glycolytic energetic state. We also showed that HH decreased apoptosis, despite a sustained ROS production and a drop of ATP amount per viable cell. The proteomic study revealed that the global protein content was better preserved in HH. This analysis identified: (i) proteins sensitive or insensitive to hypothermia irrespective of the gas phase, and (ii) proteins related to the HH cell-protective effect. Among them are some protein families known to be implicated in the prolonged survival of hibernating animals in hypothermia. These findings suggest a way to optimize short-term cell conservation without freezing. J. Cell. Physiol. 229: 2153-2165, 2014.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Hypoxia/Hypercapnia-Induced Adaptation Maintains Functional Capacity of Cord Blood Stem and Progenitor Cells at 4 degrees C",
volume = "229",
number = "12",
pages = "2153-2165",
doi = "10.1002/jcp.24678"
}