TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Betić, Nikola
AU  - Karabasil, Nedjeljko
AU  - Ćirković, Vladimir
AU  - Đurković-Đaković, Olgica
AU  - Klun, Ivana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3326
AB  - Toxoplasma gondii is a common zoonotic intracellular parasite in livestock raised for
human consumption and is a public health concern. The mode of transmission is ingestion,
and meat is considered to be a major vehicle for human and animal infection. As
T. gondii is environmentally transmissible, other important vehicles in particular for
animals include vegetation, soil and water. The seroprevalence of T. gondii infection in
pigs is Serbia has been determined in several studies over the past two decades. It has
been established that it varies considerably, primarily based on husbandry, with strictly
to mostly indoor animals having a lower prevalence (below 20%) than animals raised
outdoors, where prevalence exceeds 60%. Experimental data suggests that different
genotypes of the parasite vary in virulence, but the significance of virulence in terms of
pathology and disease manifestations is still being investigated. Genotypes of T. gondii
isolated from pig tissues in Serbia to date are ToxoDB#1 (archetype II) and ToxoDB#2
(archetype III). Archetype II is predominant and, based on historical reports and recent
findings, low to intermediately virulent. The virulence phenotype and mechanisms of
archetype III, however, have not been extensively studied, but recent data suggests that
its virulence may vary considerably. This review will also summarize the current knowledge
regarding the virulence of archetypes II and III and evaluate it in the context of
the pig host.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Toxoplasma gondii infection in pigs in Serbia
VL  - 64
IS  - 2
SP  - 82
EP  - 88
DO  - 10.18485/meattech.2023.64.2.14
ER  - 

@article{
author = "Uzelac, Aleksandra and Betić, Nikola and Karabasil, Nedjeljko and Ćirković, Vladimir and Đurković-Đaković, Olgica and Klun, Ivana",
year = "2023",
abstract = "Toxoplasma gondii is a common zoonotic intracellular parasite in livestock raised for
human consumption and is a public health concern. The mode of transmission is ingestion,
and meat is considered to be a major vehicle for human and animal infection. As
T. gondii is environmentally transmissible, other important vehicles in particular for
animals include vegetation, soil and water. The seroprevalence of T. gondii infection in
pigs is Serbia has been determined in several studies over the past two decades. It has
been established that it varies considerably, primarily based on husbandry, with strictly
to mostly indoor animals having a lower prevalence (below 20%) than animals raised
outdoors, where prevalence exceeds 60%. Experimental data suggests that different
genotypes of the parasite vary in virulence, but the significance of virulence in terms of
pathology and disease manifestations is still being investigated. Genotypes of T. gondii
isolated from pig tissues in Serbia to date are ToxoDB#1 (archetype II) and ToxoDB#2
(archetype III). Archetype II is predominant and, based on historical reports and recent
findings, low to intermediately virulent. The virulence phenotype and mechanisms of
archetype III, however, have not been extensively studied, but recent data suggests that
its virulence may vary considerably. This review will also summarize the current knowledge
regarding the virulence of archetypes II and III and evaluate it in the context of
the pig host.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Toxoplasma gondii infection in pigs in Serbia",
volume = "64",
number = "2",
pages = "82-88",
doi = "10.18485/meattech.2023.64.2.14"
}

Uzelac, A., Betić, N., Karabasil, N., Ćirković, V., Đurković-Đaković, O.,& Klun, I.. (2023). Toxoplasma gondii infection in pigs in Serbia. in Meat Technology
Institute of Meat Hygiene and Technology., 64(2), 82-88.
https://doi.org/10.18485/meattech.2023.64.2.14

Uzelac A, Betić N, Karabasil N, Ćirković V, Đurković-Đaković O, Klun I. Toxoplasma gondii infection in pigs in Serbia. in Meat Technology. 2023;64(2):82-88.
doi:10.18485/meattech.2023.64.2.14 .

Uzelac, Aleksandra, Betić, Nikola, Karabasil, Nedjeljko, Ćirković, Vladimir, Đurković-Đaković, Olgica, Klun, Ivana, "Toxoplasma gondii infection in pigs in Serbia" in Meat Technology, 64, no. 2 (2023):82-88,
https://doi.org/10.18485/meattech.2023.64.2.14 . .

TY  - JOUR
AU  - Klun, Ivana
AU  - Cirković, Vladimir
AU  - Maletić, Milan
AU  - Bradonjić, Sinisa
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1754
AB  - Neosporosis in cattle is a globally important disease, causing abortions and significant economic losses if epidemic abortions occur. In Serbia, however, studies of Neospora caninum infection in cattle are few and are based on limited samples and/or from limited areas. We thus performed a nationwide study to examine the seroprevalence of N. caninum infection in a sample of cows randomly selected from dairy farms in 12 epidemiological units from throughout Serbia, as well as the possible transmission risk factors. Sera from a total of 1496 cattle were tested by competitive ELISA, and N. caninum-specific antibodies were shown in 7.2% animals (95% confidence interval CI, 6.6-7.9%), ranging from 2.2 to 12% across the epidemiological units. At least one seropositive animal was detected on 10.7% (95% CI, 9.7-11.8%) of farms, with a range of 5.9-25.9%. Logistic regression analysis showed that the single risk factor for infection in individual animals was keeping cows in loose-stalls (OR=3.31, 95% CI=1.95-5.60, P<0.001). Risk factors for the presence of infection on farms also included housing in loose-stalls (OR=18.49, 95% CI=5.40-63.36, P<0.001), and herd size >100 animals (OR=24.08, 95% CI=3.85-150.50, P=0.001). In view of the relatively low prevalence of infection showed at both the individual and farm level, this is the perfect time to undertake appropriate preventive measures to improve animal health and reduce economic losses.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia
VL  - 118
IS  - 6
SP  - 1875
EP  - 1883
DO  - 10.1007/s00436-019-06307-9
ER  - 

@article{
author = "Klun, Ivana and Cirković, Vladimir and Maletić, Milan and Bradonjić, Sinisa and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Neosporosis in cattle is a globally important disease, causing abortions and significant economic losses if epidemic abortions occur. In Serbia, however, studies of Neospora caninum infection in cattle are few and are based on limited samples and/or from limited areas. We thus performed a nationwide study to examine the seroprevalence of N. caninum infection in a sample of cows randomly selected from dairy farms in 12 epidemiological units from throughout Serbia, as well as the possible transmission risk factors. Sera from a total of 1496 cattle were tested by competitive ELISA, and N. caninum-specific antibodies were shown in 7.2% animals (95% confidence interval CI, 6.6-7.9%), ranging from 2.2 to 12% across the epidemiological units. At least one seropositive animal was detected on 10.7% (95% CI, 9.7-11.8%) of farms, with a range of 5.9-25.9%. Logistic regression analysis showed that the single risk factor for infection in individual animals was keeping cows in loose-stalls (OR=3.31, 95% CI=1.95-5.60, P<0.001). Risk factors for the presence of infection on farms also included housing in loose-stalls (OR=18.49, 95% CI=5.40-63.36, P<0.001), and herd size >100 animals (OR=24.08, 95% CI=3.85-150.50, P=0.001). In view of the relatively low prevalence of infection showed at both the individual and farm level, this is the perfect time to undertake appropriate preventive measures to improve animal health and reduce economic losses.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia",
volume = "118",
number = "6",
pages = "1875-1883",
doi = "10.1007/s00436-019-06307-9"
}

Klun, I., Cirković, V., Maletić, M., Bradonjić, S.,& Đurković-Đaković, O.. (2019). Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia. in Parasitology Research
Springer, New York., 118(6), 1875-1883.
https://doi.org/10.1007/s00436-019-06307-9

Klun I, Cirković V, Maletić M, Bradonjić S, Đurković-Đaković O. Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia. in Parasitology Research. 2019;118(6):1875-1883.
doi:10.1007/s00436-019-06307-9 .

Klun, Ivana, Cirković, Vladimir, Maletić, Milan, Bradonjić, Sinisa, Đurković-Đaković, Olgica, "Seroprevalence of Neospora caninum infection and associated risk factors in dairy cattle in Serbia" in Parasitology Research, 118, no. 6 (2019):1875-1883,
https://doi.org/10.1007/s00436-019-06307-9 . .

TY  - JOUR
AU  - Klun, Ivana
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Vasilev, Dragan
AU  - Teodorović, Vlado
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1751
AB  - Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta veterinaria Hungarica
T1  - Trichinella spp. In wild mesocarnivores in an endemic setting
VL  - 67
IS  - 1
SP  - 34
EP  - 39
DO  - 10.1556/004.2019.004
ER  - 

@article{
author = "Klun, Ivana and Ćosić, Nada and Ćirović, Duško and Vasilev, Dragan and Teodorović, Vlado and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta veterinaria Hungarica",
title = "Trichinella spp. In wild mesocarnivores in an endemic setting",
volume = "67",
number = "1",
pages = "34-39",
doi = "10.1556/004.2019.004"
}

Klun, I., Ćosić, N., Ćirović, D., Vasilev, D., Teodorović, V.,& Đurković-Đaković, O.. (2019). Trichinella spp. In wild mesocarnivores in an endemic setting. in Acta veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(1), 34-39.
https://doi.org/10.1556/004.2019.004

Klun I, Ćosić N, Ćirović D, Vasilev D, Teodorović V, Đurković-Đaković O. Trichinella spp. In wild mesocarnivores in an endemic setting. in Acta veterinaria Hungarica. 2019;67(1):34-39.
doi:10.1556/004.2019.004 .

Klun, Ivana, Ćosić, Nada, Ćirović, Duško, Vasilev, Dragan, Teodorović, Vlado, Đurković-Đaković, Olgica, "Trichinella spp. In wild mesocarnivores in an endemic setting" in Acta veterinaria Hungarica, 67, no. 1 (2019):34-39,
https://doi.org/10.1556/004.2019.004 . .

TY  - JOUR
AU  - Ćirović, Duško
AU  - Teodorović, Vlado
AU  - Vasilev, Dragan
AU  - Marković, Marija
AU  - Ćosić, Nada
AU  - Dimitrijević, Mirjana
AU  - Klun, Ivana
AU  - Đurković-Đaković, Olgica
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1279
AB  - Over the last decades the golden jackal (Canis aureus) has significantly expanded its range throughout Southeast and Central Europe, and the Balkan Peninsula is considered to be a core area of the species distribution in this part of the range. Due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasites. The Balkans, Serbia included, remain an endemic area for various zoonotic parasites including Trichinella spp. Trichinella has recently been recorded in jackals in Serbia, which prompted us to carry out a large-scale survey of its prevalence, distribution and species identification in this host. In cooperation with local hunters, carcasses of a total of 738 legally hunted golden jackals were collected at 24 localities over an 11-year period (2003-2013). Analysis of tongue base tissue revealed Trichinella larvae in 122, indicating a prevalence of infection of 16.5%. No difference in the prevalence of infection was found between genders 116.2% in males and 16.9% in females (chi(2) = 0.05, p = 0.821)], or among the study years (G = 7.22, p = 0.705). Trichinella larvae were found in 13 out of the 24 examined localities. Molecular identification was performed for 90 isolates, and 64(71.1%) larvae were identified as Trichinella spiralis and 25(27.9%) as Trichinella britovi. Mixed infection (T. spiralis and T. britovi) was recorded in a single case. Although T. spiralis was more prevalent, T. britovi had a wider distribution, and was the only recorded species in jackal populations from the mountainous region of eastern Serbia. On the other hand, T. spiralis was dominant in jackals in the lowlands of central and northern Serbia, where domestic pigs are mostly reared. These results show that the golden jackal is involved in both the domestic and sylvatic cycle, and that it has emerged as a major host species in the sylvatic cycle of the Trichinella genus. Therefore, continued monitoring of Trichinella infection in golden jackals in Serbia and the whole of the Balkans is recommended in order to control transmission of this parasite to humans and domestic animals.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia
VL  - 212
IS  - 3-4
SP  - 253
EP  - 256
DO  - 10.1016/j.vetpar.2015.07.022
ER  - 

@article{
author = "Ćirović, Duško and Teodorović, Vlado and Vasilev, Dragan and Marković, Marija and Ćosić, Nada and Dimitrijević, Mirjana and Klun, Ivana and Đurković-Đaković, Olgica",
year = "2015",
abstract = "Over the last decades the golden jackal (Canis aureus) has significantly expanded its range throughout Southeast and Central Europe, and the Balkan Peninsula is considered to be a core area of the species distribution in this part of the range. Due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasites. The Balkans, Serbia included, remain an endemic area for various zoonotic parasites including Trichinella spp. Trichinella has recently been recorded in jackals in Serbia, which prompted us to carry out a large-scale survey of its prevalence, distribution and species identification in this host. In cooperation with local hunters, carcasses of a total of 738 legally hunted golden jackals were collected at 24 localities over an 11-year period (2003-2013). Analysis of tongue base tissue revealed Trichinella larvae in 122, indicating a prevalence of infection of 16.5%. No difference in the prevalence of infection was found between genders 116.2% in males and 16.9% in females (chi(2) = 0.05, p = 0.821)], or among the study years (G = 7.22, p = 0.705). Trichinella larvae were found in 13 out of the 24 examined localities. Molecular identification was performed for 90 isolates, and 64(71.1%) larvae were identified as Trichinella spiralis and 25(27.9%) as Trichinella britovi. Mixed infection (T. spiralis and T. britovi) was recorded in a single case. Although T. spiralis was more prevalent, T. britovi had a wider distribution, and was the only recorded species in jackal populations from the mountainous region of eastern Serbia. On the other hand, T. spiralis was dominant in jackals in the lowlands of central and northern Serbia, where domestic pigs are mostly reared. These results show that the golden jackal is involved in both the domestic and sylvatic cycle, and that it has emerged as a major host species in the sylvatic cycle of the Trichinella genus. Therefore, continued monitoring of Trichinella infection in golden jackals in Serbia and the whole of the Balkans is recommended in order to control transmission of this parasite to humans and domestic animals.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia",
volume = "212",
number = "3-4",
pages = "253-256",
doi = "10.1016/j.vetpar.2015.07.022"
}

Ćirović, D., Teodorović, V., Vasilev, D., Marković, M., Ćosić, N., Dimitrijević, M., Klun, I.,& Đurković-Đaković, O.. (2015). A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 212(3-4), 253-256.
https://doi.org/10.1016/j.vetpar.2015.07.022

Ćirović D, Teodorović V, Vasilev D, Marković M, Ćosić N, Dimitrijević M, Klun I, Đurković-Đaković O. A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia. in Veterinary Parasitology. 2015;212(3-4):253-256.
doi:10.1016/j.vetpar.2015.07.022 .

Ćirović, Duško, Teodorović, Vlado, Vasilev, Dragan, Marković, Marija, Ćosić, Nada, Dimitrijević, Mirjana, Klun, Ivana, Đurković-Đaković, Olgica, "A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia" in Veterinary Parasitology, 212, no. 3-4 (2015):253-256,
https://doi.org/10.1016/j.vetpar.2015.07.022 . .

TY  - JOUR
AU  - Teodorović, Vlado
AU  - Vasilev, Dragan
AU  - Ćirović, Duško
AU  - Marković, Marija
AU  - Ćosić, Nada
AU  - Đurić, Spomenka
AU  - Đurković-Đaković, Olgica
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1088
AB  - Wildlife is the most important reservoir of Trichinella spp. worldwide. Although the Balkans are a recognized European endemic region for Trichinella infections, data on wildlife are scarce. To monitor the circulation of these zoonotic parasites in the Central Balkan region, the wolf (Canis lupus) was selected because of its abundance (>2,000 individuals) and because it is at the top of the food chain. A total of 116 carcasses of wolves were collected in Serbia and in the neighboring areas of Macedonia and Bosnia and Herzegovina (B&H) between 2006 and 2013. Trichinella spp. larvae were found in 54 (46.5%) wolves. The great majority (90.5%) originated from Serbia, where 52 of the 105 examined animals were Trichinella positive (49.5%; 95% confidence interval =39.9-59.1). One positive animal each was found in B&H and Macedonia. All larvae were identified as Trichinella britovi. The high prevalence of Trichinella infection in wolves suggests that this carnivore can be a good indicator species for the risk assessment of the sylvatic Trichinella cycle in the Central Balkans.
PB  - Wildlife Disease Assoc, Inc, Lawrence
T2  - Journal of Wildlife Diseases
T1  - The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans
VL  - 50
IS  - 4
SP  - 911
EP  - 915
DO  - 10.7589/2013-12-333
ER  - 

@article{
author = "Teodorović, Vlado and Vasilev, Dragan and Ćirović, Duško and Marković, Marija and Ćosić, Nada and Đurić, Spomenka and Đurković-Đaković, Olgica",
year = "2014",
abstract = "Wildlife is the most important reservoir of Trichinella spp. worldwide. Although the Balkans are a recognized European endemic region for Trichinella infections, data on wildlife are scarce. To monitor the circulation of these zoonotic parasites in the Central Balkan region, the wolf (Canis lupus) was selected because of its abundance (>2,000 individuals) and because it is at the top of the food chain. A total of 116 carcasses of wolves were collected in Serbia and in the neighboring areas of Macedonia and Bosnia and Herzegovina (B&H) between 2006 and 2013. Trichinella spp. larvae were found in 54 (46.5%) wolves. The great majority (90.5%) originated from Serbia, where 52 of the 105 examined animals were Trichinella positive (49.5%; 95% confidence interval =39.9-59.1). One positive animal each was found in B&H and Macedonia. All larvae were identified as Trichinella britovi. The high prevalence of Trichinella infection in wolves suggests that this carnivore can be a good indicator species for the risk assessment of the sylvatic Trichinella cycle in the Central Balkans.",
publisher = "Wildlife Disease Assoc, Inc, Lawrence",
journal = "Journal of Wildlife Diseases",
title = "The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans",
volume = "50",
number = "4",
pages = "911-915",
doi = "10.7589/2013-12-333"
}

Teodorović, V., Vasilev, D., Ćirović, D., Marković, M., Ćosić, N., Đurić, S.,& Đurković-Đaković, O.. (2014). The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans. in Journal of Wildlife Diseases
Wildlife Disease Assoc, Inc, Lawrence., 50(4), 911-915.
https://doi.org/10.7589/2013-12-333

Teodorović V, Vasilev D, Ćirović D, Marković M, Ćosić N, Đurić S, Đurković-Đaković O. The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans. in Journal of Wildlife Diseases. 2014;50(4):911-915.
doi:10.7589/2013-12-333 .

Teodorović, Vlado, Vasilev, Dragan, Ćirović, Duško, Marković, Marija, Ćosić, Nada, Đurić, Spomenka, Đurković-Đaković, Olgica, "The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans" in Journal of Wildlife Diseases, 50, no. 4 (2014):911-915,
https://doi.org/10.7589/2013-12-333 . .