TY  - JOUR
AU  - Francuski, Jelena
AU  - Debeljak-Martačić, Jasmina
AU  - Radovanović, Anita
AU  - Andrić, Nenad
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Mojsilović, Slavko
AU  - Kovačević-Filipović, Milica
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1171
AB  - The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Proliferation and differentiation potential of canine synovial fluid cells
VL  - 65
IS  - 1
SP  - 66
EP  - 78
DO  - 10.1515/acve-2015-0005
ER  - 

@article{
author = "Francuski, Jelena and Debeljak-Martačić, Jasmina and Radovanović, Anita and Andrić, Nenad and Sourice-Petit, Sophie and Guicheux, Jerome and Mojsilović, Slavko and Kovačević-Filipović, Milica",
year = "2015",
abstract = "The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Proliferation and differentiation potential of canine synovial fluid cells",
volume = "65",
number = "1",
pages = "66-78",
doi = "10.1515/acve-2015-0005"
}

Francuski, J., Debeljak-Martačić, J., Radovanović, A., Andrić, N., Sourice-Petit, S., Guicheux, J., Mojsilović, S.,& Kovačević-Filipović, M.. (2015). Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 66-78.
https://doi.org/10.1515/acve-2015-0005

Francuski J, Debeljak-Martačić J, Radovanović A, Andrić N, Sourice-Petit S, Guicheux J, Mojsilović S, Kovačević-Filipović M. Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd. 2015;65(1):66-78.
doi:10.1515/acve-2015-0005 .

Francuski, Jelena, Debeljak-Martačić, Jasmina, Radovanović, Anita, Andrić, Nenad, Sourice-Petit, Sophie, Guicheux, Jerome, Mojsilović, Slavko, Kovačević-Filipović, Milica, "Proliferation and differentiation potential of canine synovial fluid cells" in Acta Veterinaria-Beograd, 65, no. 1 (2015):66-78,
https://doi.org/10.1515/acve-2015-0005 . .