TY  - JOUR
AU  - Nišavić, Jakov
AU  - Knežević, Aleksandra
AU  - Stanojević, M.
AU  - Milić, Nenad
AU  - Radalj, Andrea
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1645
AB  - The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.
PB  - Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3
T2  - Revue De Medecine Veterinaire
T1  - Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia
VL  - 169
IS  - 7-9
SP  - 180
EP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1645
ER  - 

@article{
author = "Nišavić, Jakov and Knežević, Aleksandra and Stanojević, M. and Milić, Nenad and Radalj, Andrea",
year = "2018",
abstract = "The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia.",
publisher = "Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3",
journal = "Revue De Medecine Veterinaire",
title = "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia",
volume = "169",
number = "7-9",
pages = "180-184",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1645"
}

Nišavić, J., Knežević, A., Stanojević, M., Milić, N.,& Radalj, A.. (2018). Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire
Ecole Nationale Veterinaire Toulouse, Toulouse Cedex 3., 169(7-9), 180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645

Nišavić J, Knežević A, Stanojević M, Milić N, Radalj A. Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia. in Revue De Medecine Veterinaire. 2018;169(7-9):180-184.
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

Nišavić, Jakov, Knežević, Aleksandra, Stanojević, M., Milić, Nenad, Radalj, Andrea, "Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia" in Revue De Medecine Veterinaire, 169, no. 7-9 (2018):180-184,
https://hdl.handle.net/21.15107/rcub_veterinar_1645 .

TY  - JOUR
AU  - Miković, Radoš
AU  - Knežević, Aleksandra
AU  - Milić, Nenad
AU  - Krnjaić, Dejan
AU  - Radojičić, Marina
AU  - Veljović, Ljubiša
AU  - Milićević, Vesna
AU  - Zorić, Andrea
AU  - Stanojević, Maja
AU  - Nišavić, Jakov
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1359
AB  - The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine.
AB  - Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro
T1  - Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori
VL  - 66
IS  - 3
SP  - 347
EP  - 358
DO  - 10.1515/acve-2016-0030
ER  - 

@article{
author = "Miković, Radoš and Knežević, Aleksandra and Milić, Nenad and Krnjaić, Dejan and Radojičić, Marina and Veljović, Ljubiša and Milićević, Vesna and Zorić, Andrea and Stanojević, Maja and Nišavić, Jakov",
year = "2016",
abstract = "The presence of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) was examined in sixty samples (spleen and lymph nodes) and thirty samples of sacral ganglia collected from non-vaccinated swine by virus isolation and polymerase chain reaction (PCR). Using PCR method PrV was detected in three samples, PPV in seven samples and six samples were found positive for PCV2. The phylogenetic analysis of the nucleotide sequences of three PrV isolates identified in this study showed high similarity and significant clustering within the PrV genotype I strains such as Kaplan and Bartha isolated from pigs in Hungary, strain Becker isolated in USA and strain Kolchis isolated in Greece. The nucleotide sequences of two PPV isolates showed high level of similarity with the strain Challenge isolated from pigs in UK, strain Kresse isolated in USA and strains 77 and LZ isolated in China. The phylogenetic analysis of the nucleotide sequences of two PCV2 isolates showed high level of similarity and significant clustering within genotype PCV2b strains such as NIVS-3, NIVS-5 and NIVS-6 isolated in Serbia, strain 3959 isolated in Austria, strain PM165 isolated from pigs in Brasil, and strain XT2008 isolated in China. The results of our study present the molecular characterization of PrV, PPV and PCV2 identified in swine in Republic of Montenegro. Besides that, these results confirmed that PCR is a very useful method for rapid detection of these viruses in subclinically infected swine., Primenom metode izolacije virusa i PCR metode, ukupno je ispitano devedeset uzoraka poreklom od svinja iz ekstenzivnog uzgoja iz različitih delova Crne Gore na prisustvo pseudorabijes virusa svinja (PrV), parvovirusa svinja (PPV) i svinjskog cirkovirusa 2 (PCV2). Primenom PCR metode prisustvo PrV je ustanovljeno kod tri uzorka, PPV kod sedam uzoraka, dok je prisustvo PCV2 utvrđeno kod šest uzoraka poreklom od svinja. Nukleotidne sekvence tri izolata PrV utvrđene u uzorcima poreklom od svinja koje su bile uključene u filogenetsku analizu su pokazale visok stepen sličnosti sa nukleotidnim sekvencama sojeva Kaplan i Bartha virusa izolovanih u Mađarskoj, zatim sa sekvencom soja Becker izolovanog u SAD-u i sekvencom soja Kolchis izolovanog u uzorcima poreklom od svinja u Grčkoj. Sva tri izolata su pripadala genotipu I pseudorabijes virusa. Nukleotidne sekvence dva izolata parvovirusa svinja su pokazale visok stepen sličnosti sa sekvencom soja Challenge izolovanog kod svinja u Velikoj Britaniji, zatim sekvencama soja Kresse izolovanog kod svinja u SAD-u i sojeva 77 i LZ izolovanih kod svinja u Kini. Filogenetska analiza nukleotidnih sekvenci dva PCV2 izolata je pokazala visok stepen sličnosti sa sojevima genotipa PCV2b kao što su NIVS-3, NIVS-5 i NIVS-6 izolovanim u Srbiji, sojem 3959 virusa izolovanim u Austriji i sojem PM165 virusa izolovanim u Brazilu i sojem XT2008 virusa izolovanim u Kini. Dobijeni rezultati ispitivanja pružili su uvid u molekularnu karakterizaciju sojeva virusa Aujeckijeve bolesti, svinjskog parvovirusa i svinjskog cirkovirusa 2 identifikovanih kod svinja u Crnoj Gori. Pored toga, dobijeni rezultati ispitivanja su potvrdili opravdanost korišćenja metode PCR u brzoj i pouzdanoj detekciji prethodno navedenih virusa kod supklinički inficiranih svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro, Molekularna detekcija PrV, PPV i PCV2 kod svinja u Republici Crnoj Gori",
volume = "66",
number = "3",
pages = "347-358",
doi = "10.1515/acve-2016-0030"
}

Miković, R., Knežević, A., Milić, N., Krnjaić, D., Radojičić, M., Veljović, L., Milićević, V., Zorić, A., Stanojević, M.,& Nišavić, J.. (2016). Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 347-358.
https://doi.org/10.1515/acve-2016-0030

Miković R, Knežević A, Milić N, Krnjaić D, Radojičić M, Veljović L, Milićević V, Zorić A, Stanojević M, Nišavić J. Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro. in Acta Veterinaria-Beograd. 2016;66(3):347-358.
doi:10.1515/acve-2016-0030 .

Miković, Radoš, Knežević, Aleksandra, Milić, Nenad, Krnjaić, Dejan, Radojičić, Marina, Veljović, Ljubiša, Milićević, Vesna, Zorić, Andrea, Stanojević, Maja, Nišavić, Jakov, "Molecular detection of pseudorabies virus (PrV), porcine parvovirus (PPV) and porcine circovirus 2 (PCV2) in swine in Republic of Montenegro" in Acta Veterinaria-Beograd, 66, no. 3 (2016):347-358,
https://doi.org/10.1515/acve-2016-0030 . .