TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Milčić Matić, Natalija
AU  - Krnjaić, Dejan
AU  - Radotić, Ksenija
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3702
AB  - Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.
PB  - Springer
T2  - Veterinary Research Communications
T1  - Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study
VL  - 48
IS  - 1
SP  - 591
EP  - 596
DO  - 10.1007/s11259-023-10224-9
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Milčić Matić, Natalija and Krnjaić, Dejan and Radotić, Ksenija",
year = "2024",
abstract = "Bacterial infections and resistance to antibiotics are increasingly severe problems. In recent years, Staphylococcus species
have emerged as important pathogens in animals and humans. Current therapeutic methods against these species have serious disadvantages; therefore new agents with antibacterial potential, such as plant-based substances, are very important in
therapy. We report a pilot study with new method of fractioning the dehydrogenate polymer DHP obtained from coniferyl
alcohol and application of the low-MW fractions of 200–3000 Da for antibacterial activity in healing animal lesions. In
vivo experiments were conducted on the dogs having a skin lesion. Dogs were treated with the suspension containing the
low-MW DHP fractions as the active ingredient, in combination with alginate for 7 days. Cytological smears and microbiological analyses of the afected area were performed. Staphylococcus spp. was isolated from lesions in all dogs from
our research. The results show that the low-MW DHP suspension in alginate promotes skin healing and reduction of the
infection of the lesions in the afected animals. Pharmaceutical composition containing the low-MW DHP fractions exerts
a soothing efect on the subject in wound treatment. Reduction in the number of bacteria by 30% and more were noticed
in 6 dogs, while in 4 dogs this percentage is above 50%. No side efects were noticed. Synthesized lignin oligomers may
have a signifcant place as antimicrobial and skin healing agents, especially since an increasing number of multidrugresistant staphylococci are found on the skin lesions in animals.",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study",
volume = "48",
number = "1",
pages = "591-596",
doi = "10.1007/s11259-023-10224-9"
}

Spasojević, D., Zmejkoski, D., Milčić Matić, N., Krnjaić, D.,& Radotić, K.. (2024). Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications
Springer., 48(1), 591-596.
https://doi.org/10.1007/s11259-023-10224-9

Spasojević D, Zmejkoski D, Milčić Matić N, Krnjaić D, Radotić K. Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study. in Veterinary Research Communications. 2024;48(1):591-596.
doi:10.1007/s11259-023-10224-9 .

Spasojević, Dragica, Zmejkoski, Danica, Milčić Matić, Natalija, Krnjaić, Dejan, Radotić, Ksenija, "Therapeutic potential of low-molecular weight lignin model polymer fractions for treating skin lesions in animals: a pilot study" in Veterinary Research Communications, 48, no. 1 (2024):591-596,
https://doi.org/10.1007/s11259-023-10224-9 . .

TY  - JOUR
AU  - Stanković, Mira
AU  - Bartolić, Dragana
AU  - Mutavdžić, Dragosav
AU  - Marković, Smilja
AU  - Grubić, Saša
AU  - Jovanović, Nemanja M.
AU  - Radotić, Ksenija
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2021
AB  - In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R2 was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.
PB  - Taylor and Francis
T2  - Journal of Apicultural Research
T1  - Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples
SP  - 1
EP  - 7
DO  - 10.1080/00218839.2021.1889803
ER  - 

@article{
author = "Stanković, Mira and Bartolić, Dragana and Mutavdžić, Dragosav and Marković, Smilja and Grubić, Saša and Jovanović, Nemanja M. and Radotić, Ksenija",
year = "2021",
abstract = "In this preliminary study, we applied the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) method to analyze the excitation-emission matrices of multifloral honey samples, combined with differential scanning calorimetry (DSC) to estimate infection of honey bee colonies with N. ceranae or V. destructor. Fluorescence spectroscopy combined with MCR-ALS was used to determine the ratio of the spectral components originating from proteins (C1) and phenolics (C2), two minor but essential constituents of honey, as a ratiometric indicator of infection level in related hives. The C1/C2 ratio decreased linearly with the increase of infection in both N. ceranae and V. destructor cases, the R2 was 0.941 and 0.912, respectively. Additionally, DSC has shown that the magnitude of changes in sugar environments of the honey samples, reflected in sugar phase transitions, rises with increasing infection level in bee colonies. These results indicate that fluorescence combined with MCR-ALS could be used for rapid, non-destructive and cheap screening of honey to estimate the level of infection of honey bee colonies.",
publisher = "Taylor and Francis",
journal = "Journal of Apicultural Research",
title = "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples",
pages = "1-7",
doi = "10.1080/00218839.2021.1889803"
}

Stanković, M., Bartolić, D., Mutavdžić, D., Marković, S., Grubić, S., Jovanović, N. M.,& Radotić, K.. (2021). Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research
Taylor and Francis., 1-7.
https://doi.org/10.1080/00218839.2021.1889803

Stanković M, Bartolić D, Mutavdžić D, Marković S, Grubić S, Jovanović NM, Radotić K. Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples. in Journal of Apicultural Research. 2021;:1-7.
doi:10.1080/00218839.2021.1889803 .

Stanković, Mira, Bartolić, Dragana, Mutavdžić, Dragosav, Marković, Smilja, Grubić, Saša, Jovanović, Nemanja M., Radotić, Ksenija, "Estimation of honey bee colony infection with Nosema ceranae and Varroa destructor using fluorescence spectroscopy in combination with differential scanning calorimetry of honey samples" in Journal of Apicultural Research (2021):1-7,
https://doi.org/10.1080/00218839.2021.1889803 . .