@article{
author = "Lužajić Božinovski, Tijana and Todorović, Vera and Milošević, Ivan and Gajdov, Vladimir and Prokić, Bogomir Bolka and Nešović, Katarina and Mišković-Stanković, Vesna and Marković, Danica",
year = "2021",
abstract = "A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue, Bilo koji novoproizvedeni biomaterijal nužno podleže standardima, po čijim se protokolima sprovode in vivo istraživanja na životinjskim modelima. Za procenu mogućnosti regeneracije mekog tkiva nakon potkožne implantacije biomaterijala - srebra/poli(vinil alkohola) (Ag/PVA) i novog materijala srebra/poli(vinil alkohola)/grafena (Ag/PVA/ Gr) predviđenog za kliničku upotrebu, šesnaest pacova je korišćeno, po uputstvima međunarodnog standarda, ISO 10993-6 2007. Histološki uzorci su sakupljeni 7, 15, 30 i 60 dana nakon implantacije. Ovi hidrogelovi su proizvedeni in situ elektrohemijskom sintezom nanočestica srebra u polimernim matricama, što je omogućilo dobijanje potpuno sigurnih i biokompatibilnih materijala, bez ikakvih dodatnih toksičnih hemijskih sredstava za redukciju. Hirurška implantacija hidrogela urađena je prema dozvoli Etičkog komiteta Fakulteta veterinarske medicine Univerziteta u Beogradu. Imunohistohemijske (IHC) studije uključivale su procenu ekspresije aktina glatkih mišića u krvnim sudovima (α-SMA), ekspresiju laminina i kolagena tipa I i III u strukturama kože i određivanje ekspresije markera proliferacije (Ki-67) keratinocita. Rezultati su procenjeni semikvantitativnom analizom. Podaci su analizirani u statističkom softverskom paketu IBM SPSS 20. Zaključci su ukazali da se Ag/PVA/Gr može koristiti kao obloga za rane kako bi se povećao potencijal zarastanja tkiva i uspostavila brža integracija i kraće zadržavanje u tkivu.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria-Beograd",
title = "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model, Procena regenerativnih procesa mekih tkiva nakon supkutane implantacije srebro / poli (vinil alkohol) i novog srebro / poli (vinil alkohol) / grafenskog hidrogela na animalnom modelu",
volume = "71",
number = "3",
pages = "285-302",
doi = "10.2478/acve-2021-0025"
}