TY  - JOUR
AU  - Tomanić, Dragana
AU  - Božin, Biljana
AU  - Čabarkapa, Ivana
AU  - Kladar, Nebojša
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Kovačević, Zorana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2458
AB  - Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.
PB  - Vaculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens
T1  - Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa
VL  - 72
IS  - 1
SP  - 45
EP  - 58
DO  - 10.2478/acve-2022-0004
ER  - 

@article{
author = "Tomanić, Dragana and Božin, Biljana and Čabarkapa, Ivana and Kladar, Nebojša and Radinović, Miodrag and Maletić, Milan and Kovačević, Zorana",
year = "2022",
abstract = "Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.",
publisher = "Vaculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens, Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa",
volume = "72",
number = "1",
pages = "45-58",
doi = "10.2478/acve-2022-0004"
}

Tomanić, D., Božin, B., Čabarkapa, I., Kladar, N., Radinović, M., Maletić, M.,& Kovačević, Z.. (2022). Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria
Vaculty of Veterinary Medicine, University of Belgrade., 72(1), 45-58.
https://doi.org/10.2478/acve-2022-0004

Tomanić D, Božin B, Čabarkapa I, Kladar N, Radinović M, Maletić M, Kovačević Z. Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria. 2022;72(1):45-58.
doi:10.2478/acve-2022-0004 .

Tomanić, Dragana, Božin, Biljana, Čabarkapa, Ivana, Kladar, Nebojša, Radinović, Miodrag, Maletić, Milan, Kovačević, Zorana, "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens" in Acta Veterinaria, 72, no. 1 (2022):45-58,
https://doi.org/10.2478/acve-2022-0004 . .

TY  - JOUR
AU  - Kovačević, Zorana
AU  - Kladar, Nebojša
AU  - Čabarkapa, Ivana
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Erdeljan, Mihajlo
AU  - Božin, Biljana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2390
AB  - Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.
PB  - MDPI
T2  - Antibiotics
T1  - New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives
VL  - 10
IS  - 12
DO  - 10.3390/antibiotics10121460
ER  - 

@article{
author = "Kovačević, Zorana and Kladar, Nebojša and Čabarkapa, Ivana and Radinović, Miodrag and Maletić, Milan and Erdeljan, Mihajlo and Božin, Biljana",
year = "2021",
abstract = "Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.",
publisher = "MDPI",
journal = "Antibiotics",
title = "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives",
volume = "10",
number = "12",
doi = "10.3390/antibiotics10121460"
}

Kovačević, Z., Kladar, N., Čabarkapa, I., Radinović, M., Maletić, M., Erdeljan, M.,& Božin, B.. (2021). New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics
MDPI., 10(12).
https://doi.org/10.3390/antibiotics10121460

Kovačević Z, Kladar N, Čabarkapa I, Radinović M, Maletić M, Erdeljan M, Božin B. New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics. 2021;10(12).
doi:10.3390/antibiotics10121460 .

Kovačević, Zorana, Kladar, Nebojša, Čabarkapa, Ivana, Radinović, Miodrag, Maletić, Milan, Erdeljan, Mihajlo, Božin, Biljana, "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives" in Antibiotics, 10, no. 12 (2021),
https://doi.org/10.3390/antibiotics10121460 . .