Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum
Само за регистроване кориснике
2017
Аутори
Trailović, SašaMarjanović, Đorđe
Vidonja-Uzelac, Teodora
Milovanović, Mirjana
Nedeljković-Trailović, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 mu M of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 mu M with R-max = 1.88 g. In the presence of... diazinon, EC50 was 12.45 mu M, while R-max was reduced to 0.43 g. After washing, the EC50 and R-max values were again closer to the control level (3.80 mu M and 1.04 g). Diazinon 1 mu M did not inhibit Ascaris suum contractions caused by ACh, but it increased the R-max. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found.
Кључне речи:
Diazinon / Muscarinic acetylcholine receptor / Ileum / Ascaris suumИзвор:
Research in Veterinary Science, 2017, 112, 18-25Издавач:
- Elsevier Sci Ltd, Oxford
Финансирање / пројекти:
DOI: 10.1016/j.rvsc.2017.01.002
ISSN: 0034-5288
PubMed: 28107667
WoS: 000403860900004
Scopus: 2-s2.0-85009518367
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Trailović, Saša AU - Marjanović, Đorđe AU - Vidonja-Uzelac, Teodora AU - Milovanović, Mirjana AU - Nedeljković-Trailović, Jelena PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1468 AB - Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 mu M of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 mu M with R-max = 1.88 g. In the presence of diazinon, EC50 was 12.45 mu M, while R-max was reduced to 0.43 g. After washing, the EC50 and R-max values were again closer to the control level (3.80 mu M and 1.04 g). Diazinon 1 mu M did not inhibit Ascaris suum contractions caused by ACh, but it increased the R-max. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found. PB - Elsevier Sci Ltd, Oxford T2 - Research in Veterinary Science T1 - Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum VL - 112 SP - 18 EP - 25 DO - 10.1016/j.rvsc.2017.01.002 ER -
@article{ author = "Trailović, Saša and Marjanović, Đorđe and Vidonja-Uzelac, Teodora and Milovanović, Mirjana and Nedeljković-Trailović, Jelena", year = "2017", abstract = "Acute poisoning with OPs may lead to a range of neurological effects, which cannot be explained by AChE inhibition alone. Several OPs interact directly with cholinergic receptors in mammals, but such data does not exist for invertebrates. The aim of current study was to investigate the direct and indirect effects of diazinon on the contractions of rat ileum and to compare those effects on the nervemuscle preparation of the Ascaris suum. In the presence of increasing concentrations of diazinon (3, 10 and 30 nM), EFS-induced ileal contractions were increased significantly. In the same preparation, diazinon 3 nM, significantly increased contractions induced by EFS, but did not affect the contractions caused by 5MFI. Contrarily, 1 mu M of diazinon significantly and reversibly inhibited the EFS-induced ileal contractions. Diazinon exhibited competitive and non-competitive inhibitions of 5MFI induced contractions. The control EC50 of 5MFI was 2.48 mu M with R-max = 1.88 g. In the presence of diazinon, EC50 was 12.45 mu M, while R-max was reduced to 0.43 g. After washing, the EC50 and R-max values were again closer to the control level (3.80 mu M and 1.04 g). Diazinon 1 mu M did not inhibit Ascaris suum contractions caused by ACh, but it increased the R-max. Diazinon in our study exhibits two opposite effects on the motor activity of the ileum. In low nanomolar concentrations the dominat is its effect on AChE and the stimulation of contractions. Furthermore, in concentrations that approach micromolar values diazinon has a direct inhibitory effect on muscarinic receptors. The direct inhibitory effect of diazinon on A. suum contractions was not found.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Research in Veterinary Science", title = "Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum", volume = "112", pages = "18-25", doi = "10.1016/j.rvsc.2017.01.002" }
Trailović, S., Marjanović, Đ., Vidonja-Uzelac, T., Milovanović, M.,& Nedeljković-Trailović, J.. (2017). Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum. in Research in Veterinary Science Elsevier Sci Ltd, Oxford., 112, 18-25. https://doi.org/10.1016/j.rvsc.2017.01.002
Trailović S, Marjanović Đ, Vidonja-Uzelac T, Milovanović M, Nedeljković-Trailović J. Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum. in Research in Veterinary Science. 2017;112:18-25. doi:10.1016/j.rvsc.2017.01.002 .
Trailović, Saša, Marjanović, Đorđe, Vidonja-Uzelac, Teodora, Milovanović, Mirjana, Nedeljković-Trailović, Jelena, "Two opposite dose-dependent effects of diazinon on the motor activity of the rat ileum" in Research in Veterinary Science, 112 (2017):18-25, https://doi.org/10.1016/j.rvsc.2017.01.002 . .