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Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
dc.creator | Danilović-Luković, Jelena | |
dc.creator | Korac, Aleksandra | |
dc.creator | Milošević, Ivan | |
dc.creator | Lužajić, Tijana | |
dc.creator | Milanović, Zorana | |
dc.creator | Kovačević-Filipović, Milica | |
dc.creator | Radovanović, Anita | |
dc.date.accessioned | 2020-06-03T14:14:52Z | |
dc.date.available | 2020-06-03T14:14:52Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0567-8315 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1477 | |
dc.description.abstract | Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy. | en |
dc.publisher | Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS// | |
dc.rights | openAccess | |
dc.source | Acta Veterinaria-Beograd | |
dc.subject | ATG7 | en |
dc.subject | caspase-3 | en |
dc.subject | LC3 | en |
dc.subject | meiosis prophase I | en |
dc.subject | secondary necrosis | en |
dc.title | Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Радовановић, Aнита; Милошевић, Иван; Лужајић, Тијана; Милановић, Зорана; Даниловић-Луковић, Јелена; Ковачевић-Филиповић, Милица; Корац, Aлександра; | |
dc.citation.volume | 67 | |
dc.citation.issue | 1 | |
dc.citation.spage | 92 | |
dc.citation.epage | 106 | |
dc.citation.other | 67(1): 92-106 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000401672900009 | |
dc.identifier.doi | 10.1515/acve-2017-0009 | |
dc.identifier.scopus | 2-s2.0-85016747573 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/437/1476.pdf | |
dc.type.version | publishedVersion |