Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
2018
Преузимање 🢃
Аутори
Milošević, IvanRadovanović, Anita
Danilović-Luković, Jelena
Lužajić Božinovski, Tijana
Sourice-Petit, Sophie
Beck-Cormier, Sarah
Guicheux, Jerome
Vejnović, Branislav
Kovačević-Filipović, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglyca...ns was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.
Кључне речи:
type 1 / 2 and 10 collagens / TRAP / DMP-1 / hypertrophic chondrocytes / offspring hypothyroidismИзвор:
Acta Veterinaria-Beograd, 2018, 68, 3, 301-320Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
- COST ActionEuropean Cooperation in Science and Technology (COST) [MP1005]
DOI: 10.2478/acve-2018-0026
ISSN: 0567-8315
WoS: 000446384500006
Scopus: 2-s2.0-85056206025
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milošević, Ivan AU - Radovanović, Anita AU - Danilović-Luković, Jelena AU - Lužajić Božinovski, Tijana AU - Sourice-Petit, Sophie AU - Beck-Cormier, Sarah AU - Guicheux, Jerome AU - Vejnović, Branislav AU - Kovačević-Filipović, Milica PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1588 AB - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation VL - 68 IS - 3 SP - 301 EP - 320 DO - 10.2478/acve-2018-0026 ER -
@article{ author = "Milošević, Ivan and Radovanović, Anita and Danilović-Luković, Jelena and Lužajić Božinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Vejnović, Branislav and Kovačević-Filipović, Milica", year = "2018", abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein-1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation", volume = "68", number = "3", pages = "301-320", doi = "10.2478/acve-2018-0026" }
Milošević, I., Radovanović, A., Danilović-Luković, J., Lužajić Božinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J., Vejnović, B.,& Kovačević-Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 301-320. https://doi.org/10.2478/acve-2018-0026
Milošević I, Radovanović A, Danilović-Luković J, Lužajić Božinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Vejnović B, Kovačević-Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320. doi:10.2478/acve-2018-0026 .
Milošević, Ivan, Radovanović, Anita, Danilović-Luković, Jelena, Lužajić Božinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Vejnović, Branislav, Kovačević-Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320, https://doi.org/10.2478/acve-2018-0026 . .