Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1
Само за регистроване кориснике
2018
Аутори
Bartolić, DraganaStanković, Mira
Mutavdžić, Dragosav
Stanković, Slavica
Jovanović, Dragoljub
Radotić, Ksenija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks positions were red-shifted. We found that the ratio ...of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.
Кључне речи:
Steady state fluorescence spectroscopy / Excitation-emission matrix / MCR-ALS / Aflatoxin B1 / Maize flourИзвор:
Journal of Fluorescence, 2018, 28, 3, 729-733Издавач:
- Springer/Plenum Publishers, New York
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.1007/s10895-018-2246-z
ISSN: 1053-0509
PubMed: 29934744
WoS: 000440111700002
Scopus: 2-s2.0-85048897572
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Bartolić, Dragana AU - Stanković, Mira AU - Mutavdžić, Dragosav AU - Stanković, Slavica AU - Jovanović, Dragoljub AU - Radotić, Ksenija PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1655 AB - In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour. PB - Springer/Plenum Publishers, New York T2 - Journal of Fluorescence T1 - Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1 VL - 28 IS - 3 SP - 729 EP - 733 DO - 10.1007/s10895-018-2246-z ER -
@article{ author = "Bartolić, Dragana and Stanković, Mira and Mutavdžić, Dragosav and Stanković, Slavica and Jovanović, Dragoljub and Radotić, Ksenija", year = "2018", abstract = "In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.", publisher = "Springer/Plenum Publishers, New York", journal = "Journal of Fluorescence", title = "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1", volume = "28", number = "3", pages = "729-733", doi = "10.1007/s10895-018-2246-z" }
Bartolić, D., Stanković, M., Mutavdžić, D., Stanković, S., Jovanović, D.,& Radotić, K.. (2018). Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1. in Journal of Fluorescence Springer/Plenum Publishers, New York., 28(3), 729-733. https://doi.org/10.1007/s10895-018-2246-z
Bartolić D, Stanković M, Mutavdžić D, Stanković S, Jovanović D, Radotić K. Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1. in Journal of Fluorescence. 2018;28(3):729-733. doi:10.1007/s10895-018-2246-z .
Bartolić, Dragana, Stanković, Mira, Mutavdžić, Dragosav, Stanković, Slavica, Jovanović, Dragoljub, Radotić, Ksenija, "Multivariate Curve Resolution - Alternate Least Square Analysis of Excitation-Emission Matrices for Maize Flour Contaminated with Aflatoxin B1" in Journal of Fluorescence, 28, no. 3 (2018):729-733, https://doi.org/10.1007/s10895-018-2246-z . .