Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis
2019
Аутори
Stevanović, IvanaMancić, Bojana
Ilić, Tihomir
Milosavljević, Petar
Lavrnja, Irena
Stojanović, Ivana
Ninković, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freunds adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After ...decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rats spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions.
Кључне речи:
experimental autoimmune encephalomyelitis (EAE) / spinal cord / TMS / BDNFИзвор:
Folia Neuropathologica, 2019, 57, 2, 129-145Издавач:
- Termedia Publishing House Ltd, Poznan
Финансирање / пројекти:
- University of Defence [MFVMA/04/19-21]
- Превентивни, терапијски и етички приступ преклиничким и клиничким истраживањима гена и модулатора редокс ћелијске сигнализације у имунском, инфламаторном и пролиферативном одговору ћелије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41018)
DOI: 10.5114/fn.2019.86294
ISSN: 1641-4640
PubMed: 31556573
WoS: 000475371600004
Scopus: 2-s2.0-85072664097
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stevanović, Ivana AU - Mancić, Bojana AU - Ilić, Tihomir AU - Milosavljević, Petar AU - Lavrnja, Irena AU - Stojanović, Ivana AU - Ninković, Milica PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1750 AB - Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freunds adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rats spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions. PB - Termedia Publishing House Ltd, Poznan T2 - Folia Neuropathologica T1 - Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis VL - 57 IS - 2 SP - 129 EP - 145 DO - 10.5114/fn.2019.86294 ER -
@article{ author = "Stevanović, Ivana and Mancić, Bojana and Ilić, Tihomir and Milosavljević, Petar and Lavrnja, Irena and Stojanović, Ivana and Ninković, Milica", year = "2019", abstract = "Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in the activity of excitatory and inhibitory systems, restores these functions and suppresses the progression of disability in experimental autoimmune encephalitis (EAE). The structural type of TMS, the arrangement as theta burst stimulation (TBS) has been applied as intermittent TBS (iTBS) and continuous TBS (cTBS) protocols to female adult DA rats. The animals were randomly divided into experimental groups: control group (C), group treated with complete Freunds adjuvant (CFA), experimental autoimmune encephalomyelitis (EAE) group, group treated with iTBS post EAE immunization (EAE + iTBS), group treated with cTBS post EAE immunization (EAE + cTBS), group of healthy animals treated with iTBS or cTBS. Therapeutic protocols of iTBS or cTBS in all EAE groups of animals were performed starting from 14 days post immunization (dpi), for 10 days with time point decapitation at 24 dpi. After decapitation, spinal cords were analysed for BDNF and Ki67 expression. The results revealed reduced BDNF expression in the rats spinal cord of EAE animals in the stage of remission, which was associated with increased Ki67 and GFAP expressions. Decreased Iba 1 and BDNF expression, contrary to increased Iba 1 and Ki67 expression, suggests clustered microglia in the resolution phase of EAE. Enhanced GABA expression in spinal cord sections indicates higher GABA metabolic turnover, and also GAD activity in astrocytes, or prominent activity of GABAergic neurons. Both TBS protocols induced advance BDNF expression; amongst iTBS application provoked elevating of BDNF and stabilizing of GFAP and Ki67 expressions.", publisher = "Termedia Publishing House Ltd, Poznan", journal = "Folia Neuropathologica", title = "Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis", volume = "57", number = "2", pages = "129-145", doi = "10.5114/fn.2019.86294" }
Stevanović, I., Mancić, B., Ilić, T., Milosavljević, P., Lavrnja, I., Stojanović, I.,& Ninković, M.. (2019). Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis. in Folia Neuropathologica Termedia Publishing House Ltd, Poznan., 57(2), 129-145. https://doi.org/10.5114/fn.2019.86294
Stevanović I, Mancić B, Ilić T, Milosavljević P, Lavrnja I, Stojanović I, Ninković M. Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis. in Folia Neuropathologica. 2019;57(2):129-145. doi:10.5114/fn.2019.86294 .
Stevanović, Ivana, Mancić, Bojana, Ilić, Tihomir, Milosavljević, Petar, Lavrnja, Irena, Stojanović, Ivana, Ninković, Milica, "Theta burst stimulation influence the expression of BDNF in the spinal cord on the experimental autoimmune encephalomyelitis" in Folia Neuropathologica, 57, no. 2 (2019):129-145, https://doi.org/10.5114/fn.2019.86294 . .