Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
Само за регистроване кориснике
2019
Аутори
Živković, LadaBajić, Vladan
Bruić, Marija
Borozan, Sunčica
Popić, Kristina
Topalović, Dijana
Santibanez, Juan Francisco
Spremo-Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage... after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cells repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
Кључне речи:
Immune Assist (IA) / Antigenotoxicity / center dot OH Scavenging propertiesИзвор:
Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2019, 845, UNSP 403078-Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-173034)
- hCOMET COST actionEuropean Cooperation in Science and Technology (COST) [15132]
DOI: 10.1016/j.mrgentox.2019.06.008
ISSN: 1383-5718
PubMed: 31561902
WoS: 000489193400018
Scopus: 2-s2.0-85070315567
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Živković, Lada AU - Bajić, Vladan AU - Bruić, Marija AU - Borozan, Sunčica AU - Popić, Kristina AU - Topalović, Dijana AU - Santibanez, Juan Francisco AU - Spremo-Potparević, Biljana PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1790 AB - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cells repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed. PB - Elsevier, Amsterdam T2 - Mutation Research-Genetic Toxicology and Environmental Mutagenesis T1 - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study VL - 845 SP - UNSP 403078 DO - 10.1016/j.mrgentox.2019.06.008 ER -
@article{ author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan Francisco and Spremo-Potparević, Biljana", year = "2019", abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 mu g/mL, while in posttreatment it was the concentration of 250 mu g/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15-60 min), reaching the greatest reduction after 15 and 45 min. Remarkable center dot OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cells repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.", publisher = "Elsevier, Amsterdam", journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis", title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study", volume = "845", pages = "UNSP 403078", doi = "10.1016/j.mrgentox.2019.06.008" }
Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J. F.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis Elsevier, Amsterdam., 845, UNSP 403078. https://doi.org/10.1016/j.mrgentox.2019.06.008
Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez JF, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 403078. doi:10.1016/j.mrgentox.2019.06.008 .
Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan Francisco, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 403078, https://doi.org/10.1016/j.mrgentox.2019.06.008 . .