Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
2019
Аутори
Topalović, DijanaDekanski, Dragana
Spremo-Potparević, Biljana
Pirković, Andrea
Borozan, Sunčica
Bajić, Vladan
Stojanović, Danilo
Giampieri, Francesca
Gasparrini, Massimiliano
Živković, Lada
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17 beta-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 mu M (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenoto...xic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells antioxidant capacity.
Кључне речи:
DOLE / 17 beta-estradiol / DES / Antigenotoxicity / Comet assayИзвор:
Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2019, 845, UNSP 402993-Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- hCOMET COST actionEuropean Cooperation in Science and Technology (COST) [15132]
DOI: 10.1016/j.mrgentox.2018.12.001
ISSN: 1383-5718
PubMed: 31561897
WoS: 000489193400019
Scopus: 2-s2.0-85059148840
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Topalović, Dijana AU - Dekanski, Dragana AU - Spremo-Potparević, Biljana AU - Pirković, Andrea AU - Borozan, Sunčica AU - Bajić, Vladan AU - Stojanović, Danilo AU - Giampieri, Francesca AU - Gasparrini, Massimiliano AU - Živković, Lada PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1791 AB - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17 beta-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 mu M (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells antioxidant capacity. PB - Elsevier, Amsterdam T2 - Mutation Research-Genetic Toxicology and Environmental Mutagenesis T1 - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro VL - 845 SP - UNSP 402993 DO - 10.1016/j.mrgentox.2018.12.001 ER -
@article{ author = "Topalović, Dijana and Dekanski, Dragana and Spremo-Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada", year = "2019", abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17 beta-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 mu M (P < 0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P < 0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells antioxidant capacity.", publisher = "Elsevier, Amsterdam", journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis", title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro", volume = "845", pages = "UNSP 402993", doi = "10.1016/j.mrgentox.2018.12.001" }
Topalović, D., Dekanski, D., Spremo-Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis Elsevier, Amsterdam., 845, UNSP 402993. https://doi.org/10.1016/j.mrgentox.2018.12.001
Topalović D, Dekanski D, Spremo-Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 402993. doi:10.1016/j.mrgentox.2018.12.001 .
Topalović, Dijana, Dekanski, Dragana, Spremo-Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 402993, https://doi.org/10.1016/j.mrgentox.2018.12.001 . .