Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom
Antinflammatory effect of thiamine in the brain of Japanese quails (Coturnix japonica) treated with chlorpyrifos
Authors
Ćupić Miladinović, DejanaBorozan, Sunčica
Đorđević, Milena
Blagojević, Miloš
Nešić, Ivana
Cukić, Nikola
Ivanović, Saša
Ćupić, Vitomir
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Cilj ovog istraživanja bio je da se ispita uticaj vitamina B1 (tiamina) na inflamatorne promene u mozgu japanskih prepelica tretiranih hlorpirifosom. Ispitivani su sledeći inflamatorni parametri: koncentracija vodonik-peroksida–H2O2, aktivnost mijeloperoksidaze–MPO, aktivnost ciklooksigenaze–COX i interleukina–1 (IL–1) i interleukina–6 (IL–6).
Istraživanje je sprovedeno na osamdeset mužjaka japanskih prepelica (2 kontrolne grupe i 6 eksperimentalnih grupa, n= 10), starosti 3-4 nedelje. Jedna kontrolna grupa je dobijala samo vitamin B1, dok je druga primala čisto kukuruzno ulje. CPF rastvoren u kukuruznom ulju davan je tri grupe prepelica putem sonde tokom 7 uzastopnih dana u dozama od 1,5 mg/kg TM, 3 mg/kg TM i 6 mg/kg TM. Druge tri grupe su tretirane sa 10 mg/kg TM vitamina B1 i.m. 30 min nakon primene CPF-a (u gore navedenim dozama) tokom 7 uzastopnih dana.
Aktivnost MPO i koncentracija H2O2 merene su spektrofotometrijski korišćenjem Cecil CE 2021 UV/VIS spektrofotometra. Za dete...kciju COX koristili smo Western blot analizu, dok su IL-1 i IL-6 određeni komercijalnim ELISA testom (enzimski imunosorbentni test).
Naša studija je pokazala da je CPF doveo do povećanja koncentracije H2O2, aktivnosti MPO, ali je nakon tretmana tiaminom došlo do smanjenja ovih parametara. Povećanje COX nakon tretmana CPF pokazuje povećanje inflamatorne reakcije i ranjivost ćelija izloženih CPF-u, dok je vitamin B1 uzrokovao smanjenje njihove ekspresije. Takođe CPF stimuliše odgovor imunog sistema povećanjem lučenja IL-1 i IL-6, dok B1 smanjuje proizvodnju ovih proinflamatornih citokina.
Sve u svemu, ovi rezultati daju nove uvide u istraživanje tiamina kao „protivupalnog vitamina“, tako da su dalja istraživanja tiamina kao potencijalnog terapeutikapotrebna i opravdana.
The aim of this study was to investigate the influence of vitamin B1 (thiamine) on inflammatory changes in the brain of Japanese quail treated with chlorpyrifos. The following inflammatoryparameterswere examined: concentration of hydrogen peroxide¬–H2O2,activity of myeloperoxidase–MPO, activity of cyclooxygenase–COX and interleukins–1 (IL–1) and interleukins–6(IL–6).
The study was conducted on eighty male Japanese quails (2 control groups and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil.CPFdissolved in corn oil was administeredto three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days.
Activity of MPO and concentration of H2O2was measured spectrophotometricallyusing a Cecil CE 2021 UV/V...IS spectrophotometer. For detection of COX we used Western blot analysis, while IL-1 and IL-6 were determined with a commercial ELISA test (Enzyme linked Immunosorbent Assay).
Our study have shown thatCPF has led to increase in the concentration of H2O2, activity of MPO, but after thiamine treatment there has been a decrease of these parameters.Increase of COXafter CPF treatment demonstrates an increase of inflammatory reaction and vulnerability of cells exposed to CPF, while vitamin B1 caused a decrease in their expression.Also CPF stimulates the responseof the immune system by increasing secretion of IL-1 and IL-6, while B1 lowers the production of these proinflamatory cytokines.
Overall these results are giving new insights intothiamine research as an "anti-inflammatory vitamin", so further investigations of thiamine as a potential therapeutic are well deserved and justified.
Keywords:
hlorpirifos / tiamin / inflamatorna reakcija / chlorpyrifos / thiamine / inflammatory reactionSource:
28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023, 2023, 195-197Publisher:
- Banja Luka : Veterinarska komora Republike Srpske
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
Note:
- Zbornik radova i kratkih sadržaja Proceedings and short contents
Collections
Institution/Community
Fakultet veterinarske medicineTY - CONF AU - Ćupić Miladinović, Dejana AU - Borozan, Sunčica AU - Đorđević, Milena AU - Blagojević, Miloš AU - Nešić, Ivana AU - Cukić, Nikola AU - Ivanović, Saša AU - Ćupić, Vitomir PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2969 AB - Cilj ovog istraživanja bio je da se ispita uticaj vitamina B1 (tiamina) na inflamatorne promene u mozgu japanskih prepelica tretiranih hlorpirifosom. Ispitivani su sledeći inflamatorni parametri: koncentracija vodonik-peroksida–H2O2, aktivnost mijeloperoksidaze–MPO, aktivnost ciklooksigenaze–COX i interleukina–1 (IL–1) i interleukina–6 (IL–6). Istraživanje je sprovedeno na osamdeset mužjaka japanskih prepelica (2 kontrolne grupe i 6 eksperimentalnih grupa, n= 10), starosti 3-4 nedelje. Jedna kontrolna grupa je dobijala samo vitamin B1, dok je druga primala čisto kukuruzno ulje. CPF rastvoren u kukuruznom ulju davan je tri grupe prepelica putem sonde tokom 7 uzastopnih dana u dozama od 1,5 mg/kg TM, 3 mg/kg TM i 6 mg/kg TM. Druge tri grupe su tretirane sa 10 mg/kg TM vitamina B1 i.m. 30 min nakon primene CPF-a (u gore navedenim dozama) tokom 7 uzastopnih dana. Aktivnost MPO i koncentracija H2O2 merene su spektrofotometrijski korišćenjem Cecil CE 2021 UV/VIS spektrofotometra. Za detekciju COX koristili smo Western blot analizu, dok su IL-1 i IL-6 određeni komercijalnim ELISA testom (enzimski imunosorbentni test). Naša studija je pokazala da je CPF doveo do povećanja koncentracije H2O2, aktivnosti MPO, ali je nakon tretmana tiaminom došlo do smanjenja ovih parametara. Povećanje COX nakon tretmana CPF pokazuje povećanje inflamatorne reakcije i ranjivost ćelija izloženih CPF-u, dok je vitamin B1 uzrokovao smanjenje njihove ekspresije. Takođe CPF stimuliše odgovor imunog sistema povećanjem lučenja IL-1 i IL-6, dok B1 smanjuje proizvodnju ovih proinflamatornih citokina. Sve u svemu, ovi rezultati daju nove uvide u istraživanje tiamina kao „protivupalnog vitamina“, tako da su dalja istraživanja tiamina kao potencijalnog terapeutikapotrebna i opravdana. AB - The aim of this study was to investigate the influence of vitamin B1 (thiamine) on inflammatory changes in the brain of Japanese quail treated with chlorpyrifos. The following inflammatoryparameterswere examined: concentration of hydrogen peroxide¬–H2O2,activity of myeloperoxidase–MPO, activity of cyclooxygenase–COX and interleukins–1 (IL–1) and interleukins–6(IL–6). The study was conducted on eighty male Japanese quails (2 control groups and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil.CPFdissolved in corn oil was administeredto three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Activity of MPO and concentration of H2O2was measured spectrophotometricallyusing a Cecil CE 2021 UV/VIS spectrophotometer. For detection of COX we used Western blot analysis, while IL-1 and IL-6 were determined with a commercial ELISA test (Enzyme linked Immunosorbent Assay). Our study have shown thatCPF has led to increase in the concentration of H2O2, activity of MPO, but after thiamine treatment there has been a decrease of these parameters.Increase of COXafter CPF treatment demonstrates an increase of inflammatory reaction and vulnerability of cells exposed to CPF, while vitamin B1 caused a decrease in their expression.Also CPF stimulates the responseof the immune system by increasing secretion of IL-1 and IL-6, while B1 lowers the production of these proinflamatory cytokines. Overall these results are giving new insights intothiamine research as an "anti-inflammatory vitamin", so further investigations of thiamine as a potential therapeutic are well deserved and justified. PB - Banja Luka : Veterinarska komora Republike Srpske C3 - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 T1 - Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom T1 - Antinflammatory effect of thiamine in the brain of Japanese quails (Coturnix japonica) treated with chlorpyrifos SP - 195 EP - 197 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2969 ER -
@conference{ author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Đorđević, Milena and Blagojević, Miloš and Nešić, Ivana and Cukić, Nikola and Ivanović, Saša and Ćupić, Vitomir", year = "2023", abstract = "Cilj ovog istraživanja bio je da se ispita uticaj vitamina B1 (tiamina) na inflamatorne promene u mozgu japanskih prepelica tretiranih hlorpirifosom. Ispitivani su sledeći inflamatorni parametri: koncentracija vodonik-peroksida–H2O2, aktivnost mijeloperoksidaze–MPO, aktivnost ciklooksigenaze–COX i interleukina–1 (IL–1) i interleukina–6 (IL–6). Istraživanje je sprovedeno na osamdeset mužjaka japanskih prepelica (2 kontrolne grupe i 6 eksperimentalnih grupa, n= 10), starosti 3-4 nedelje. Jedna kontrolna grupa je dobijala samo vitamin B1, dok je druga primala čisto kukuruzno ulje. CPF rastvoren u kukuruznom ulju davan je tri grupe prepelica putem sonde tokom 7 uzastopnih dana u dozama od 1,5 mg/kg TM, 3 mg/kg TM i 6 mg/kg TM. Druge tri grupe su tretirane sa 10 mg/kg TM vitamina B1 i.m. 30 min nakon primene CPF-a (u gore navedenim dozama) tokom 7 uzastopnih dana. Aktivnost MPO i koncentracija H2O2 merene su spektrofotometrijski korišćenjem Cecil CE 2021 UV/VIS spektrofotometra. Za detekciju COX koristili smo Western blot analizu, dok su IL-1 i IL-6 određeni komercijalnim ELISA testom (enzimski imunosorbentni test). Naša studija je pokazala da je CPF doveo do povećanja koncentracije H2O2, aktivnosti MPO, ali je nakon tretmana tiaminom došlo do smanjenja ovih parametara. Povećanje COX nakon tretmana CPF pokazuje povećanje inflamatorne reakcije i ranjivost ćelija izloženih CPF-u, dok je vitamin B1 uzrokovao smanjenje njihove ekspresije. Takođe CPF stimuliše odgovor imunog sistema povećanjem lučenja IL-1 i IL-6, dok B1 smanjuje proizvodnju ovih proinflamatornih citokina. Sve u svemu, ovi rezultati daju nove uvide u istraživanje tiamina kao „protivupalnog vitamina“, tako da su dalja istraživanja tiamina kao potencijalnog terapeutikapotrebna i opravdana., The aim of this study was to investigate the influence of vitamin B1 (thiamine) on inflammatory changes in the brain of Japanese quail treated with chlorpyrifos. The following inflammatoryparameterswere examined: concentration of hydrogen peroxide¬–H2O2,activity of myeloperoxidase–MPO, activity of cyclooxygenase–COX and interleukins–1 (IL–1) and interleukins–6(IL–6). The study was conducted on eighty male Japanese quails (2 control groups and 6 experimental groups, n= 10), 3-4 weeks old. One control group was treated only with vitamin B1, while the second one received pure corn oil.CPFdissolved in corn oil was administeredto three groups of quails by gavage for 7 consecutive days at doses of 1.5 mg/kg BW, 3 mg/kg BW and 6 mg/kg BW. Another three groups were treated with 10 mg/kg BWof vitamin B1 i.m. 30 min after CPF administration (in above mentioned doses) for 7 consecutive days. Activity of MPO and concentration of H2O2was measured spectrophotometricallyusing a Cecil CE 2021 UV/VIS spectrophotometer. For detection of COX we used Western blot analysis, while IL-1 and IL-6 were determined with a commercial ELISA test (Enzyme linked Immunosorbent Assay). Our study have shown thatCPF has led to increase in the concentration of H2O2, activity of MPO, but after thiamine treatment there has been a decrease of these parameters.Increase of COXafter CPF treatment demonstrates an increase of inflammatory reaction and vulnerability of cells exposed to CPF, while vitamin B1 caused a decrease in their expression.Also CPF stimulates the responseof the immune system by increasing secretion of IL-1 and IL-6, while B1 lowers the production of these proinflamatory cytokines. Overall these results are giving new insights intothiamine research as an "anti-inflammatory vitamin", so further investigations of thiamine as a potential therapeutic are well deserved and justified.", publisher = "Banja Luka : Veterinarska komora Republike Srpske", journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023", title = "Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom, Antinflammatory effect of thiamine in the brain of Japanese quails (Coturnix japonica) treated with chlorpyrifos", pages = "195-197", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2969" }
Ćupić Miladinović, D., Borozan, S., Đorđević, M., Blagojević, M., Nešić, I., Cukić, N., Ivanović, S.,& Ćupić, V.. (2023). Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 Banja Luka : Veterinarska komora Republike Srpske., 195-197. https://hdl.handle.net/21.15107/rcub_veterinar_2969
Ćupić Miladinović D, Borozan S, Đorđević M, Blagojević M, Nešić I, Cukić N, Ivanović S, Ćupić V. Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:195-197. https://hdl.handle.net/21.15107/rcub_veterinar_2969 .
Ćupić Miladinović, Dejana, Borozan, Sunčica, Đorđević, Milena, Blagojević, Miloš, Nešić, Ivana, Cukić, Nikola, Ivanović, Saša, Ćupić, Vitomir, "Antinflamatorni efekat tiamina u tkivu mozga kod Japanskih prepelica (Coturnix japonica) tretiranih hlorpirifosom" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):195-197, https://hdl.handle.net/21.15107/rcub_veterinar_2969 .