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Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo
dc.creator | Bajić, Vladan | |
dc.creator | Stanimirović, Zoran | |
dc.creator | Stevanović, Jevrosima | |
dc.date.accessioned | 2020-06-03T12:52:52Z | |
dc.date.available | 2020-06-03T12:52:52Z | |
dc.date.issued | 2004 | |
dc.identifier.issn | 0188-4409 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/306 | |
dc.description.abstract | Background. Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3,5-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo. To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods. Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results. Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (4.88 +/- 0.35, 8.32 +/- 0.57, and 11.74 +/- 0.37) compared to negative control (2.04 +/- 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions. Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo. | en |
dc.publisher | Elsevier Science Inc, New York | |
dc.rights | restrictedAccess | |
dc.source | Archives of Medical Research | |
dc.subject | antitumor agent 8-Cl-cAMP | en |
dc.subject | genotoxicity | en |
dc.subject | micronucleus test | en |
dc.subject | numerical and structural chromosomal aberrations | en |
dc.title | Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Бајић, В; Станимировић, Зоран; Стевановић, Јевросима; | |
dc.citation.volume | 35 | |
dc.citation.issue | 3 | |
dc.citation.spage | 209 | |
dc.citation.epage | 214 | |
dc.citation.other | 35(3): 209-214 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000221906400004 | |
dc.identifier.doi | 10.1016/j.arcmed.2004.01.004 | |
dc.identifier.pmid | 15163461 | |
dc.identifier.scopus | 2-s2.0-2442421673 | |
dc.type.version | publishedVersion |