Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity
Аутори
Ristanić, MarkoVučković, Jelena
Dominiković, Nina
Vučićević, Miloš
Rajković, Milan
Bogunović, Danica
Özvegy, József
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Protozoa of the genus Giardia cause the disease giardiosis in a large number of animal
species, but also in humans. This disease can be either subclinical or followed by diarrhea,
malabsorption, weight loss and stunted growth. The aim of this study was to examine the
presence of Giardia sp. in different species of animals in Belgrade Zoo (asymptomatic
infection) and to compare the sensitivity of parasitological and molecular-genetic
methods. Examination of the presence of Giardia sp. protozoa was performed in 52
fecal samples, using fecal fl otation and molecular-genetic PCR method. No positive
samples were detected when deploying the parasitological method, while PRC analysis
revealed the presence of Giardia sp. in one animal - ring-tailed lemur (Lemur catta),
which indicates the advantage of the molecular-genetic method over the parasitological
one. The absence of Giardia sp. in other samples can be explained by adequate housing
conditions, successful implementation of hyg...ienic and sanitary measures, as well as
good veterinary practice. Use of PCR method for testing the presence of Giardia sp.
showed a higher sensitivity when compared to the fecal fl otation method.
Кључне речи:
Flotation / Giardia / PCR / Zoo animalsИзвор:
Acta Veterinaria, 2023, 73, 2, 195-204Издавач:
- Beograd : Fakultet veterinarske medicine
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Ristanić, Marko AU - Vučković, Jelena AU - Dominiković, Nina AU - Vučićević, Miloš AU - Rajković, Milan AU - Bogunović, Danica AU - Özvegy, József PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3140 AB - Protozoa of the genus Giardia cause the disease giardiosis in a large number of animal species, but also in humans. This disease can be either subclinical or followed by diarrhea, malabsorption, weight loss and stunted growth. The aim of this study was to examine the presence of Giardia sp. in different species of animals in Belgrade Zoo (asymptomatic infection) and to compare the sensitivity of parasitological and molecular-genetic methods. Examination of the presence of Giardia sp. protozoa was performed in 52 fecal samples, using fecal fl otation and molecular-genetic PCR method. No positive samples were detected when deploying the parasitological method, while PRC analysis revealed the presence of Giardia sp. in one animal - ring-tailed lemur (Lemur catta), which indicates the advantage of the molecular-genetic method over the parasitological one. The absence of Giardia sp. in other samples can be explained by adequate housing conditions, successful implementation of hygienic and sanitary measures, as well as good veterinary practice. Use of PCR method for testing the presence of Giardia sp. showed a higher sensitivity when compared to the fecal fl otation method. PB - Beograd : Fakultet veterinarske medicine T2 - Acta Veterinaria T1 - Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity VL - 73 IS - 2 SP - 195 EP - 204 DO - 10.2478/acve-2023-0015 ER -
@article{ author = "Ristanić, Marko and Vučković, Jelena and Dominiković, Nina and Vučićević, Miloš and Rajković, Milan and Bogunović, Danica and Özvegy, József", year = "2023", abstract = "Protozoa of the genus Giardia cause the disease giardiosis in a large number of animal species, but also in humans. This disease can be either subclinical or followed by diarrhea, malabsorption, weight loss and stunted growth. The aim of this study was to examine the presence of Giardia sp. in different species of animals in Belgrade Zoo (asymptomatic infection) and to compare the sensitivity of parasitological and molecular-genetic methods. Examination of the presence of Giardia sp. protozoa was performed in 52 fecal samples, using fecal fl otation and molecular-genetic PCR method. No positive samples were detected when deploying the parasitological method, while PRC analysis revealed the presence of Giardia sp. in one animal - ring-tailed lemur (Lemur catta), which indicates the advantage of the molecular-genetic method over the parasitological one. The absence of Giardia sp. in other samples can be explained by adequate housing conditions, successful implementation of hygienic and sanitary measures, as well as good veterinary practice. Use of PCR method for testing the presence of Giardia sp. showed a higher sensitivity when compared to the fecal fl otation method.", publisher = "Beograd : Fakultet veterinarske medicine", journal = "Acta Veterinaria", title = "Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity", volume = "73", number = "2", pages = "195-204", doi = "10.2478/acve-2023-0015" }
Ristanić, M., Vučković, J., Dominiković, N., Vučićević, M., Rajković, M., Bogunović, D.,& Özvegy, J.. (2023). Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity. in Acta Veterinaria Beograd : Fakultet veterinarske medicine., 73(2), 195-204. https://doi.org/10.2478/acve-2023-0015
Ristanić M, Vučković J, Dominiković N, Vučićević M, Rajković M, Bogunović D, Özvegy J. Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity. in Acta Veterinaria. 2023;73(2):195-204. doi:10.2478/acve-2023-0015 .
Ristanić, Marko, Vučković, Jelena, Dominiković, Nina, Vučićević, Miloš, Rajković, Milan, Bogunović, Danica, Özvegy, József, "Use of parasitological and molecular methods in Giardia sp. detection in animals held in captivity" in Acta Veterinaria, 73, no. 2 (2023):195-204, https://doi.org/10.2478/acve-2023-0015 . .