“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis
Samo za registrovane korisnike
2023
Autori
Rajković, MilanGlavinić, Uroš
Bogunović, Danica
Vejnović, Branislav
Davitkov, Darko
Đelić, Ninoslav
Stanimirović, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various
molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim
of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of
sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole
blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure
and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote,
Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used
in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating
microfilari...ae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA
damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe
clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all
groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that
leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the
modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from
the dogs’ blood.
Ključne reči:
Heartworm disease / Microfilariae / DNA damage / Modified Knott’s test / PCRIzvor:
Veterinary Parasitology, 2023, 322, 110008-Izdavač:
- Elsevier
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200143 (Univerzitet u Beogradu, Fakultet veterinarske medicine) (RS-MESTD-inst-2020-200143)
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Rajković, Milan AU - Glavinić, Uroš AU - Bogunović, Danica AU - Vejnović, Branislav AU - Davitkov, Darko AU - Đelić, Ninoslav AU - Stanimirović, Zoran PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3191 AB - Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote, Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from the dogs’ blood. PB - Elsevier T2 - Veterinary Parasitology T1 - “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis VL - 322 SP - 110008 DO - 10.1016/j.vetpar.2023.110008 ER -
@article{ author = "Rajković, Milan and Glavinić, Uroš and Bogunović, Danica and Vejnović, Branislav and Davitkov, Darko and Đelić, Ninoslav and Stanimirović, Zoran", year = "2023", abstract = "Parasitic diseases are considered to be a cause of oxidative stress which leads to oxidative damage of various molecules including DNA. This can result in mutations, replication errors, and genome instability. Therefore, aim of this study was to measure DNA damage induced by Dirofilaria immitis in the single cells such as dogs’ leukocytes using the comet assay. Also, we monitored the effects of antiparasitic treatment on mitigation of sensitivity to DNA damage in leukocytes treated with H2O2 using the in vivo and ex vivo comet assay. The whole blood samples from 34 dogs from Serbia were used, both males and females, from one to 13 years old, both pure and mixed-breeds. A rapid immunochromatographic test (Antigen Rapid Heartworm Ag 2.0 Test Kit, Bionote, Minnesota, USA) was used for the detection of D. immitis antigens. The modified Knott’s test and PCR were used in the aim of detecting D. immitis microfilariae in dogs’ blood, and evaluating the number of circulating microfilariae during the treatment. The genotoxicity evaluation showed that D. immitis infection resulted in DNA damage in naturally infected dogs, with the highest DNA damage occurring in the group of dogs with severe clinical signs. Treatment with ivermectin and doxycycline decreased DNA damage in leukocytes of dogs in all groups, as the intensity of infection decreased due to applied therapy. Ex vivo comet assay results showed that leukocytes exhibited decreased sensitivity to H2O2-induced DNA damage during treatment. The results of the modified Knott’s test and PCR in our study showed that treatment with ivermectin and doxycycline was successful in decreasing the average number of microfilariae during the time and at the end eliminating them from the dogs’ blood.", publisher = "Elsevier", journal = "Veterinary Parasitology", title = "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis", volume = "322", pages = "110008", doi = "10.1016/j.vetpar.2023.110008" }
Rajković, M., Glavinić, U., Bogunović, D., Vejnović, B., Davitkov, D., Đelić, N.,& Stanimirović, Z.. (2023). “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology Elsevier., 322, 110008. https://doi.org/10.1016/j.vetpar.2023.110008
Rajković M, Glavinić U, Bogunović D, Vejnović B, Davitkov D, Đelić N, Stanimirović Z. “Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis. in Veterinary Parasitology. 2023;322:110008. doi:10.1016/j.vetpar.2023.110008 .
Rajković, Milan, Glavinić, Uroš, Bogunović, Danica, Vejnović, Branislav, Davitkov, Darko, Đelić, Ninoslav, Stanimirović, Zoran, "“Slow kill” treatment reduces DNA damage in leukocytes of dogs naturally infected with Dirofilaria immitis" in Veterinary Parasitology, 322 (2023):110008, https://doi.org/10.1016/j.vetpar.2023.110008 . .